Shift in Adaptive Immune Response Types in Dry-Eye Disease
description
Transcript of Shift in Adaptive Immune Response Types in Dry-Eye Disease
Shift in Adaptive Immune Response Types in Dry-Eye Disease
Yihe Chen, Sunil K Chauhan, Reza DanaSchepens Eye Research Institute
Harvard Medical SchoolBoston, MA
The authors have no financial interest in the subject matter of this poster
Background
• Dry eye disease (DED)– An inflammatory autoimmune disorder– T cell involvement in the pathogenesis
Characterization of adaptive immune response during DED course would be relevant to the development of more effective therapies
Purpose
Determine kinetics of generation of adaptive immunity in DED
Methods
[Controlled-environment chamber]
DED induction
2 or 7 days
Conjunctiva
Cervical lymph nodesTissue harvest
PCR FCM
Analysis of immune
response
ResultsIFN-IFN-γγ and IL-17 mRNA levels and IL-17 mRNA levels
Conjunctiva Draining lymph nodes
0
1
2
3
Day 2 Day7
Fo
ld c
ha
ng
e i
n m
RN
A e
xp
res
sio
n
IFN-γ
IL-17
0
1
2
3
Day 2 Day7
Fo
ld c
ha
ng
e i
n m
RN
A e
xp
res
sio
n
IFN-γ
IL-17
NL NL
NL: mRNA level in normal mice; * p < 0.05 compared with normal
* * *
*
Both the conjunctiva and draining lymph nodes of DED mice showed an enhanced expression of IFN-γ mRNA on day 2 while a substantially increased expression of IL-17 mRNA on day 7
ResultsTh1 and Th17 cellsTh1 and Th17 cells
CD4
IL-1
7
Th1/17 cell is defined as IFN-γ+ / IL-17+ CD4+ population; * p < 0.05 compared with normal
Day 2 Day 7
IFN
-γ
Th1
Th17
*
*
•Th1 cells: significantly increased on day 2, and then diminished to the baseline level on day 7
•Th17 cells: no significant change on day 2, but significantly increased on day 7
Conclusion
These findings suggest:
- the adaptive immune response in early DED is characterized by a Th1 phenotype, whereas,
-the progression of DED is characterized by the Th17 response