Several Factors Regulate Glycosylation of Glycoproteins
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Transcript of Several Factors Regulate Glycosylation of Glycoproteins
Glycosyltransferase Family Donor Substrate
Fucosyltransferase GDP-Fucose
Sialyltransferase CMP-Neuraminic acid
Galactosyltransferase UDP-Galactose
N-Acetyl glucosaminyl transferase UDP- N-Acetyl glucosamin
N-Acetyl galactosaminyl transferase UDP- N-Acetyl galactosamin
Mannosyltransferase GDP-Mannose
Glucosyltransferase UDP-Glucose
Suitable acceptor sites in proteins.
The tissue level of Dol-P.
The activity of the oligosaccharide:protein transferase.
Different cells give different enzymes.
Certain glycosyltransferases act only on the oligosaccaride chain if it has already been acted
upon by another previous enzyme.
EX: action of GlcNAc is important for Golgi alpha-mannosaidase.
During which some genes are turned on & others are turned off.
Each enzyme has its own destination for insertion.
Ex:HMG.CoA reductase ER membrane it may never encounter Golgi-located processing enzyme.
Differences in proteins conformation may hinder access of proccessing
enzymes.
•The same cells(ex:fibroblast) of different species exhibit
different patterns of processing enzymes.
•Species variation is an important factor which is used for production of Glycoproteins
of Therapeutic use by Recombinant DNA tech.
EX:synthesizedErythropoietin (EPO)
1. Synthesized EPO is administered to patients with chronic anemia in order to stimulate Erythropoiesis.
2. The half-life of EPO in plasma is influenced by the nature of its glycosylation pattern.
3. Some species give patterns with short HL, others give them with normal HL.
1• By lysosomal acid hydrolases.
2• They are Exo-enzymes.
3
• If anyone is missing ,degradation can’t
complete>>>>Leading to diseases!