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Session 2: New methods/developments in

seed sampling ISTA Sampling Seminar

June 16, 2014 Edinburgh, Scotland, UK

Steve Jones Unit Head, CFIA

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Sampling Seminar: Session 2

Canadian Food Agence canadienne

Inspection Agency d’inspection des aliments

Seed Science & Technology Section, Saskatoon

Bonjour

Welcome to Scotland & Session 2

Saskatoon landscape courtesy and property of Tourism Saskatoon (see www.tourismsaskatoon.com)

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Session 2: Content

Chair: Steve Jones

• Validation of new methods and equipment for sampling purposes: Steve Jones

• Optimizing sampling plans for seed transmitted pathogens: Roy MacArthur and

Valerie Cockerell

• Automatic seed sampling: Lotta Claesson

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Session 2: Content

Validation of new methods and equipment for sampling purposes

Validation or standardisation?

• Standardisation since 1869, Nobbe • Validation has been worked on since the 1900’s • ISTA validated methods since formation in 1924 • First edition of the ISTA Rules in 1931

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Method validation

ISTA Rules first edition 1931

Now electronic since 2014

http://www.ingentaconnect.com/content/ista/rules

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Method validation

Five W’s and one H

I keep six honest serving-men (They taught me all I knew); Their names are What and Why and When And How and Where and Who. ………. Rudyard Kipling, “Just So Stories”, 1902

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Method validation

• Why validate new methods or equipment? • What to validate? • How to validate? • Who does the work? • When is it done? • Where is it presented?

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Method validation

Why validate new methods or equipment?

The need for:

• Consistency • Uniformity • Reproducibility • Repeatability • Trueness

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Method validation

What needs to be validated? Any new methods to be used as a standard method in the ISTA Rules

Approved equipment referenced in the ISTA Rules

For sampling for example: Nobbe triers are approved

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Method validation

How to get a method or a piece of equipment approved for use in the ISTA Rules? Method validation (70 page document) Since 2002 there has been a formal validation requirement for all new methods & equipment

http://www.seedtest.org/en/method-validation-programme-_content---1--1254--465.html

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Method validation

Process covered in detail in the document • Multi-laboratory validation/comparison • Peer laboratory validation/comparison • Sponsor • Test organiser • Approved test plan • Statistical analysis • Scientific reviewers

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Method validation

In the last 10 years there has been one validation report for new sampling equipment (2009) The new equipment was voted on in 2010 and came into effect in the 2011 Edition of the ISTA Rules

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Method validation

This leads onto the Who, When and Where?

Who does the work? Interested parties and/or ISTA Technical Committee

For sampling: Bulking & Sampling TCOM But before the work starts a validation project plan with an approved design and analysis is required.

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Method validation

Who? • An important part of who does the work are the

interested parties • Proposing lab needs to be involved in the study • Do not underestimate the workload • More than one laboratory? • Different seed lots and samples? • Data analysis and co-ordination roles

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Method validation

When all the validation work has been done and the report completed:

• Reviewed by the ISTA Statistics TCOM • Reviewed by members of TCOM or other experts • Rules proposal along with supporting validation

report submitted to the ISTA Rules TCOM Chair

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Method validation

Where? Once a Rules proposal supported by a validation report has been submitted presented at the annual voting meeting

When? This year’s proposals are being voted on in a few days time at ISTA’s annual voting meeting 2014

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Method validation

Past example from the 2009 validation report: Spiral sampler Cargo sampler • One laboratory • Two seed plants • Different seed lots

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Method validation

Report structure

• Overview of objectives • Results • Statistical analysis • Discussion • Recommendation: progress or not? • Rules proposal

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Method validation

Validation report gives: • Statistical analysis • Transparency • Scientific based decisions • Peer reviewed

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Method validation

Past example from the 2009 validation report: Spiral sampler Cargo sampler

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Method validation

Past example from the 2009 validation report: Spiral sampler & Cargo sampler, compared to:

the approved ISTA double sleeved sampler with compartments

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Method validation

Results: For seeds smaller than Triticum all three sampling devices were within acceptable limits of variation

Conclusion: The spiral sampler and cargo sampler were added to the ISTA Rules

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Method validation

What could be new methods or equipment for sampling?

• New auto samplers • New designs of riffles • Use existing sampling devices in new ways • Different dividers • Artificial materials for verification/calibration • Orobanche spp. and mixture sampling methods Something else ???

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Method validation

This completes the overview • More specifics on new developments in sampling

from the next presenters • Summary at the end of the session

Thank you for now and any questions?

Image courtesy of JoJ Images © 2013