Serial Dilutions
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![Page 1: Serial Dilutions](https://reader036.fdocuments.net/reader036/viewer/2022062321/5681351e550346895d9c7d42/html5/thumbnails/1.jpg)
Serial DilutionsSerial Dilutions
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Get 2 x 3 inch jewelry
bags jewelry supply store. See
product # 52-002 on second table @
http://www.aajewelry.com/templates/product_list_dropdown_pix.aspx?GroupGuid=5523
Cut piece of paper towel and insert into bag
Staple across the bag such that there is
space for roots to grow
Add 10 to 15 seeds to each
bag
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0.2 g/mL NaCl0.2 g/mL NaCl
Stock SolutionStock Solution
Make a 0.2-g/mL NaClNaCl STOCKSTOCK solutionsolution
- Add 2 g of sodium chloride to beaker and add water to 10 mL
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Get as many test tubes (minus two controls) as you have plastic
jewelry bags for your experiment
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Add 5 mL of water to each of you test tubes
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Add 5 mL of Stock Solution to
first test tube.
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Mix the first Tube
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Take 5 mL of first tube and add to second
tube
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Mix
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Repeat and remove 5 ml
from last tube and discard
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Using a 5mL disposable pipette 1.Add 3 mL from stock to a bag
2.Add 3 mL of water to another bag 3.Add the 3 mL from each test tube to
its respective bag
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Stock SolutionStock Solution
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Stoc
k
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WaterWater
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Wat
er
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0.1 g/mL 0.1 g/mL SolutionSolution
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0.1
g/m
L
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0.05 g/mL 0.05 g/mL SolutionSolution
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0.5
g/m
L
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EtcEtc..
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Let Grow in Dark for ~ 1 week
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