Sequence Tracking
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Sequence Tracking Deanna M. Church Staff Scientist, NCBI @deannachurch Short Course in Medical Genetics nderstanding your sequence context
description
Sequence Tracking. Understanding your sequence context. Deanna M. Church Staff Scientist, NCBI. Short Course in Medical Genetics 2013. @ deannachurch. What’s in a name?. Bob. Bob. Bob. Bob. What’s in a name?. Bob. *. 123-45-6789. *http://howmanyofme.com. What’s in a name?. Bob. - PowerPoint PPT Presentation
Transcript of Sequence Tracking
Sequence TrackingDeanna M. Church Staff Scientist, NCBI
@deannachurch Short Course in Medical Genetics 2013
Understanding your sequence context
What’s in a name?Bob Bob
BobBob
Bob
*
*http://howmanyofme.com
What’s in a name?
123-45-6789
Bob
MirandaLydia
Samantha
What’s in a name?
Need more than unique identifiertrack updates/improvements
chr1Chr11Chrom1
Mouse chrX: 34,800,000-34,890,000
NC_000086.123456 CM001013.17 2
Mouse chrX: 35,000,000-36,000000
X
MGSCv3 MGSCv36
GenBank
Data Archives
Data in a common formatData in a single location (and mirrored)Most quality checked prior to depositionRobust data tracking mechanism (accession.version)Data owned by submitter
Data tracking ABC14-1065514J1
GapsPhase LengthDate
FP565796.1 1 121-Oct-2009
FP565796.2 1 014-Oct-2010
FP565796.3 3 007-Nov-2010
Data Archives
Initial versions of human and mouse reference assemblies not in INSDC!!*
First human version in INSDC: GRCh37First mouse version in INSDC: NCBI36
* But were tracked by RefSeq
Data ArchivesINSDC archives track INDIVIDUAL sequences
An assembly is a COLLECTION of sequences
hg19GRCh37
mm8MGSCv37
NCBIM37
danRer5Zv7
More naming issues
chr21:8,913,216-9,246,964
Zv7
Zv7 chr21:8,913,216-9,246,964 vs MGSCv36 chrX
http://www.ncbi.nlm.nih.gov/genome/assembly
GRCh37hg19
Genome Browser AgreementSubmitter deposits
assembly to GenBank/EMBL/DDBJ
Assembly QA
Submitter updates assembly based on QA
results
Browsers pick up assembly from
GenBank/EMBL/DDBJ Assemblies must be in GenBank/EMBL/DDBJ
GenBank RefSeq vs
Submitter Owned RefSeq OwnedRedundancy Non-Redundant
Updated rarely CuratedINSDC Not INSDC
BRCA183 genomic records31 mRNA records27 protein records
3 genomic records 5 mRNA records1 RNA record5 protein records
RefSeq for Assemblies
Typical assembly edits
Addition of non-nuclear (e.g. MT) assembly units
Removal of contamination
Drop unlocalized/unplaced scaffoldsMask contamination that is placed on chromosome(while preserving coordinate space)
http://www.ncbi.nlm.nih.gov/assembly/organism/9606/
Human assemblies in assembly database
Take home messages
Assemblies can (and do) update!Know what assembly your are working on
Track by accession.version, not just nameData in INSDC databases are mirroredRefSeq is NCBI specific
![Motion Segmentation & Multiple Object Tracking by ......Left: Frames 100, 110, and 120 of the sequence MOT16-08 [50]. Right: Segmentation and tracking result are depicted as color-labeled](https://static.fdocuments.net/doc/165x107/5f537d93b93fe8613a385b8c/motion-segmentation-multiple-object-tracking-by-left-frames-100.jpg){kind=tracking}
