ReversedPhaseColumnScreener

Critical Pair Characteristics

Application

USP Classification

Recommended Alternative

EaSily SElECt by

and gEt a guaRantEEd Column Solution.

ReversedPhaseColumnScreener

Critical Pair Characteristics

Application

USP Classification

Recommended Alternative

EaSily SElECt by

and gEt a guaRantEEd Column Solution.

Now there’s an online column screening application that allows you to instantly narrow down to the perfect reversed phase column for your method. Find your ideal column match based on:

Phenomenex l WEB: www.phenomenex.com

At the Forefront of Separation Science Technology

Special Edition

Phenomenex

Phenomenex Creates Reversed Phase Column Screener and Online Application

Phenomenex, Inc. introduces the Reversed Phase Column Screener, a resource that helps chromatographers select the most effective HPLC or UHPLC column for their methods from the company’s extensive offering. Based on decades of customer support and research, the Reversed Phase Column Screener allows users to search by compound characteristics, pharmacopeia classification, column used, or application.

When the chromatographer enters compound characteristics, the screener and web tool will provide primary and secondary recommendations for fully porous particle columns with complementary selectivities, and a core-shell particle option for ultra-high performance. Recommended mobile phase starting conditions are also provided. Users may also search by USP classification to determine which columns they can use to stay within method guidelines.

ColumnMatch.com was developed as a web application tool in correlation to the handheld screener. This web application instantly sorts through available selectivities and finds the right column for your methods. The comprehensive tool provides a convenient way to reference application notes on the Phenomenex website. Hard copies of the RP Column Screener can also be accessed online at www.ColumnMatch.com.

Separation Science Singapore 2011

“As the only manufacturer to provide fully porous, core-shell, and monolithic reversed phase columns, our offering has grown to the point where users may need help in determining which option will give them the very best results. We have used our experience to create an effective tool that allows chromatographers to conveniently and easily find the ideal reversed phase column for their method.”

USP Classification

Application

Recommended Alternative

Compound

Separation Science Singapore 2011

Page 2 of 4 www.phenomenex.com

Polymeric SPE Sorbent Technology for Higher Recovery

Most traditional solid phase extraction sorbents on the market are just variations of the same technology, and often result in poor analyte recoveries, insufficient cleanup, or irreproducibility from extraction to extraction. By contrast, Strata-X sorbents have been developed using revolutionary polymeric technology and are designed to eliminate the common bottlenecks associated with traditional SPE sorbents.

With Strata-X polymeric sorbents the guesswork that leads to lengthy method development and complications of screening multiple SPE sorbents is eliminated. Five unique selectivities, each available in 33 µm or 100 µm particle sizes, as well as 1, 3,

and 6 mL tubes, Giga™ tubes, 96-well plates, and online cartridges, have been developed to cover a diverse spectrum of analytes and simplify the method development process for fast and efficient sample preparation.

A carefully controlled manufacturing process combined with deconditioning resistant sorbent beds and strong retention mechanisms ensure that Strata-X sorbents provide better reproducibility from extraction to extraction.

“To date we have already developed more than 700 methods using this tool.”

New Online SPE Method Development Tool

www.phenomenex.com/info/MDTool

Phenomenex recently introduced a new SPE Method Development tool. This new online resource can create customized SPE methods in under 1 minute for all types of sample matrices, analytes, and volumes.

Lux® Chiral Columns Make It Easy

SIMPLIFIED CHIRAL SEPARATIONS

Lux columns offer a wide range of functionalized polysaccharide-based supports that provide enantioselectivity for even the most difficult chiral separations. Lux columns offer stability within normal phase, polar organic, SFC, and reversed phase conditions, and are offered in 3 μm and 5 μm packed columns and 20 μm bulk media for scale-up. With pressure stability up to 300 bar, Lux maintains high analytical and preparative efficiencies and loading capacity. With five complementary and orthogonal chiral selectivities and exceptional in-house technical expertise, you are sure to find enantiomeric separation success in your lab.

Phenomenex offers FREE Chiral Screening services worldwide through PhenoLogixSM, our in-house analytical support laboratory. Our team provides full support for labs needing method development and optimization, scale-up and purification, and on-site training & consulting.

“We’re now using half the reagents, half the time, and half the price—with triple the results! We’re getting chromatography that is sharp and clean, with improved resolution and recovery. This is a revolutionary product for analysis.” Meredith Wilson, Dynacare Labs, AL, USA.

www.phenomenex.com/phenologix

Separation Science Singapore 2011

Page 3 of 4www.phenomenex.com

Phenomenex Adds Two New Phases to Kinetex® Core-Shell HPLC/UHPLC Column Line

Kinetex XB-C18 and Kinetex C8 bring the advantages of ultra-high liquid chromatography performance (UHPLC) within standard HPLC

operating pressures to a growing list of applications. Kinetex

core-shell technology meets researchers’ need to improve results and increase productivity without large capital expenditures.

The new Kinetex XB-C18 provides a unique selectivity incorporating two iso-butyl side chains at the base of the C18 ligand to reduce secondary interactions with residual silanols. These columns deliver enhanced separation and peak shape of basic compounds under neutral and acidic pH conditions. In comparison studies with other C18 columns, this new Kinetex phase has produced differences in peak elution

order and improved separation of co-eluting compounds.

The new Kinetex C8 phase meets the United States Pharmacopeia (USP) specifications for Classification Code L7, enabling laboratories using L7 columns to realize the benefits of Kinetex core-shell technology while staying compliant with USP-allowable adjustments.

The new Kinetex core-shell columns are avai lable in two particle sizes 2 .6- and 1 .7- micron – and in a variety of column dimensions.

“We introduced our revolutionary Kinetex Core-Shell technology two years ago, and the market response and adoption rate have exceeded our expectations. We’re pleased to add these two new phases to the three we initially offered. Our product development team will continue to bring even more core-shell resources to the chromatography field.”

Gemini®-NX was engineered to be the column of choice for pharmaceutical scientists who work with difficult sample mixtures and harsh mobile phase conditions that demand a rugged column and long lifetime.

Many of today’s pharmaceutical compounds possess one or more ionizable functional groups, so it is essential to understand the impact ionized compounds may have on chromatographic results. Ionized compounds will display different solubility and hydrophobicity characteristics when compared to their neutral state, often resulting in poor chromatographic results in the most common reversed phase HPLC conditions.

Separation scientists can expect Gemini-NX to overcome pH limitations previously encountered with silica and hybrid particles. This technology allows you to control ionization states of solutes by altering mobile phase pH. The result is improved performance for analytical and preparative-scale separations from pH 1-12.

www.phenomenex.com, keyword: Kinetex

E x t e n d e d C o l u m n Lifetime Under Extreme pH Coditions (pH 1-12)

www.phenomenex.com, keyword: Gemini

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www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com.

Trademarks Kinetex and Lux are registered trademark of Phenomenex in the United States, European Union, and other jurisdictions. Zebron, Strata-X, and Gemini-NX, Giga, ColumnMatch.com, Ph-LC, and Inferno are trade-marks of Phenomenex. Apple and iPad are trademarks of Apple Inc., registered in the U.S. and other countries.

Strata-X is patented by Phenomenex, Inc. U.S. Patent No. 7,119,145.Gemini-NX is patented by Phenomenex, Inc. U.S. Patent No. 7, 563, 367

Disclaimers Phenomenex, Inc. and Omega, are in no way affiliated with Apple. Apple is not a sponsor of this promotion.

© 2011 Phenomenex, Inc. All rights reserved.

Come Visit the Phenomenex Booth for a Chance to WIN

an Apple® iPad®!

Zebron™ Inferno™ GC Columns Bring High Temperature Stability to Mid-Polarity Analyses

Zebron has added two new mid-polarity phases to its Inferno line of high temperature GC columns. The ZB-XLB-HT and ZB-35HT are the first mid-polarity columns able to withstand temperatures up to 400 °C, enabling analyses of compounds with high molecular weights and boiling points. These fused-silica phases are stable at high temperatures due to proprietary coating and binding technologies, providing long lifetime, low bleed, and low activity. They are ideal for environmental, food and beverage, pharmaceutical, toxicology, and biofuels applications.

Standard fused-silica columns are not engineered to withstand temperatures above 380 °C and their polyimide coating begins to degrade, eventually becoming brittle and inflexible. Zebron Inferno columns are specially designed for high temperature analysis, with more rugged coating and stationary phases.

The ZB-XLB-HT Inferno column is stable up to 400 °C and provides unique selectivity for conformational analyses. This MS-certified column offers low activity and good peak shape for acidic and basic samples. This proprietary phase is ideal for polychlorinated biphenyls (PCBs), pesticides, herbicides, and many EPA methods.

The ZB-35HT is a 35 %-phenyl-65 %-dimethylpolysiloxane intermediate-polarity column that is stable up to 400 °C, enabling high molecular weight analysis. It is particularly well suited for trace analysis with bleed-sensitive detectors. Applications include semi-volatile compounds, pesticides, drugs of abuse, pharmaceutical, steroids, and many EPA methods.

“Our successful Zebron columns were the first

non-metal columns to provide stability at very high

temperatures. The increased column stability can be beneficial even for routine temperature ramps above 320 °C, which can cause some GC columns to become brittle and produce high bleed.”

AUTHORIZED DISTRIBUTOR

OMEGA SCIENTIFIC PTE LTD1 Kaki Bukit View #05m-15/16 Techview Singapore 415941Tel: +65-6744-6645 • Fax: +65-6744-9958 Email: sales@omegascientific.com.sg

Need Applications? We have thousands.www.phenomenex.com/application