Selected Case Studies
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Transcript of Selected Case Studies
Selected Case Studies
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MIT 2006: Engineering bacteria to smell good
BSMTwintergreen
C. breweri S. cerevisia
e
ATF1banana
Slides borrowed from the 2006 MIT Team
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Engineering the scent of bacteria
What is the desired output?
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Components of engineered cell
What is needed to create the desired output?
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Components of engineered cell
What is needed to create the desired output?
Creating devices with BioBricks
Regulatory Part/Promoter
Protein Coding Part
TerminatorRibosome Binding Site
Protein
Components of scent-producing devices
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Components of scent-producing devices
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Components of upstream devices
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How do you regulate when the scents are expressed?
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Goal: Sense the growth state of culture
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Regulating the timing of expression
osmY: active in stationary phase & under high osmotic pressure conditions
osmY
osmY + inverter
Regulating the timing of expression
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Design principles
• What is the desired output? What is needed to create this output?
• What do you want to sense?
• Use of constitutive promoters, inducible promoters, and inverters
Berkeley 2006Bacterial Networks
Need: To transfer DNA messages from one bacterial cell to another
Means: Bacterial Conjugation
Need: To specifically control who can read the DNA message
Means: Riboregulation
Some slides borrowed from the 2006 Berkeley Team
Regulatory Part/Promoter
Terminator
Protein
Protein Coding PartRibosome Binding
Site
Ribosome Binding Site
Protein
DNA
RNARibosome Binding
Site
DNA to RNA to Protein
Protein synthesis from RNA
Protein
RNARibosome Binding
Site
Ribosome
Locked structure prevents ribosome binding
Protein
RNARibosome Binding
Site
Ribosome
RNA “Key” unlocks lock
Protein
RNARibosome Binding
Site
Ribosome
Goal: Create network of cell-cell communication
RNA as a regulatory mechanism
• Can activate some “riboswitches” with small molecules
• Advantage: fast response - transcript is already made
• Alternative method: block translation by adding antisense RNA to RBD