SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY.
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SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
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Transcript of SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY.
SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING
ACTIVITY
MICROTUBULES
HeLa
10-15 pf
FUNCTIONS OF TUBULINMICROTUBULEMT-ROLES
INTRACELLULARTRANSPORT
CELL SHAPEAND MIGRATION
MITOSIS CILIA amp FLAGELLA
TUBULIN - TARGET
MICROTUBULE
HETERODIMER
DESTABILIZING AGENTSINHIBIT POLYMERIZATION
DISASSEMBLE MICROTUBULES
STABILIZING AGENTSALTER MICROTUBULE
DYNAMICS
bull MITOTIC ARREST ANTI-PROLIFERATIVE ACTIVITYbull SHUTDOWN OF TUMOR BLOOD SUPPLY
DESTABSTRUCTURES
S
O
N
NH
NH OCH3
O
N
NHO
O
N
Cl
NH
N
OH
N
H3CO
O
H3CO N
OH
O
O
O OCH3
H
H3CO
H3CO
H3COOH
H3CO
S
N
NH
O
OCH3
NH
OH3CO
NHNH
O
O
N
H3CO
H3CO
H3CO
OCH3
O
NHCH3
O
O
O
O
O
OH
OCH3
OCH3H3CO
CH3
H
H
H
OH
H3CO
OH
NH
OO
OH
COLCHICINEPODOFYLLOTOXIN COMBRETASTATIN
D-24851
NOCODAZOLE
DOLASTATIN VINBLASTINE
NOSTODIONE
2-METHOXY-ESTRADIOL
STABILIZERSNH
O
OH
O
OH
AcO O OH
O
AcOOO
H
OO
OH
H
OH
OH
OH O
O
NH2
+
+
Diaz et al J Biol Chem 2000
S
NO
O OH
OOHO
TAXOL
DISCODERMOLIDE
EPOTHILONES
NEW ANTI-TUB DRUGS
COMPANY NAME MOLECULE BINDING SITE CLINICAL TRIALS
Asta Medica AG D-24851 New Phase II
Astra Zeneca ZD-6186 Colchicine Phase II
Aventis AVE-8062 Colchicine Phase II
OxiGene Combretastatine 4P Colchicine Phase II
Abbott ABT-751 Colchicine Phase I
Wyeth HTI-286 Colchicine Phase I
EntrMed 2-Methoxyestradiol Colchicine Phase III
Aventis LIT-976 taxoid Taxol Phase III
Bayer Taxane Bay-59-8862 Taxol Phase III
BMS Taxanes BMS247550 Taxol Phase III
Wyeth Taxane Mac 321 Taxol Phase III
BMS Epothilones Taxol Phase III
KosanRoche Epothilone KOS862 Taxol Phase III
SheringMetagen Epothilones Taxol Phase III
Novartis Epothilone EPO-906 Taxol Phase III
TUBULIN DESTABILIZERS (7)
TUBULIN STABILIZERS (8)
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
MICROTUBULES
HeLa
10-15 pf
FUNCTIONS OF TUBULINMICROTUBULEMT-ROLES
INTRACELLULARTRANSPORT
CELL SHAPEAND MIGRATION
MITOSIS CILIA amp FLAGELLA
TUBULIN - TARGET
MICROTUBULE
HETERODIMER
DESTABILIZING AGENTSINHIBIT POLYMERIZATION
DISASSEMBLE MICROTUBULES
STABILIZING AGENTSALTER MICROTUBULE
DYNAMICS
bull MITOTIC ARREST ANTI-PROLIFERATIVE ACTIVITYbull SHUTDOWN OF TUMOR BLOOD SUPPLY
DESTABSTRUCTURES
S
O
N
NH
NH OCH3
O
N
NHO
O
N
Cl
NH
N
OH
N
H3CO
O
H3CO N
OH
O
O
O OCH3
H
H3CO
H3CO
H3COOH
H3CO
S
N
NH
O
OCH3
NH
OH3CO
NHNH
O
O
N
H3CO
H3CO
H3CO
OCH3
O
NHCH3
O
O
O
O
O
OH
OCH3
OCH3H3CO
CH3
H
H
H
OH
H3CO
OH
NH
OO
OH
COLCHICINEPODOFYLLOTOXIN COMBRETASTATIN
D-24851
NOCODAZOLE
DOLASTATIN VINBLASTINE
NOSTODIONE
2-METHOXY-ESTRADIOL
STABILIZERSNH
O
OH
O
OH
AcO O OH
O
AcOOO
H
OO
OH
H
OH
OH
OH O
O
NH2
+
+
Diaz et al J Biol Chem 2000
S
NO
O OH
OOHO
TAXOL
DISCODERMOLIDE
EPOTHILONES
NEW ANTI-TUB DRUGS
COMPANY NAME MOLECULE BINDING SITE CLINICAL TRIALS
Asta Medica AG D-24851 New Phase II
Astra Zeneca ZD-6186 Colchicine Phase II
Aventis AVE-8062 Colchicine Phase II
OxiGene Combretastatine 4P Colchicine Phase II
Abbott ABT-751 Colchicine Phase I
Wyeth HTI-286 Colchicine Phase I
EntrMed 2-Methoxyestradiol Colchicine Phase III
Aventis LIT-976 taxoid Taxol Phase III
Bayer Taxane Bay-59-8862 Taxol Phase III
BMS Taxanes BMS247550 Taxol Phase III
Wyeth Taxane Mac 321 Taxol Phase III
BMS Epothilones Taxol Phase III
KosanRoche Epothilone KOS862 Taxol Phase III
SheringMetagen Epothilones Taxol Phase III
Novartis Epothilone EPO-906 Taxol Phase III
TUBULIN DESTABILIZERS (7)
TUBULIN STABILIZERS (8)
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
FUNCTIONS OF TUBULINMICROTUBULEMT-ROLES
INTRACELLULARTRANSPORT
CELL SHAPEAND MIGRATION
MITOSIS CILIA amp FLAGELLA
TUBULIN - TARGET
MICROTUBULE
HETERODIMER
DESTABILIZING AGENTSINHIBIT POLYMERIZATION
DISASSEMBLE MICROTUBULES
STABILIZING AGENTSALTER MICROTUBULE
DYNAMICS
bull MITOTIC ARREST ANTI-PROLIFERATIVE ACTIVITYbull SHUTDOWN OF TUMOR BLOOD SUPPLY
DESTABSTRUCTURES
S
O
N
NH
NH OCH3
O
N
NHO
O
N
Cl
NH
N
OH
N
H3CO
O
H3CO N
OH
O
O
O OCH3
H
H3CO
H3CO
H3COOH
H3CO
S
N
NH
O
OCH3
NH
OH3CO
NHNH
O
O
N
H3CO
H3CO
H3CO
OCH3
O
NHCH3
O
O
O
O
O
OH
OCH3
OCH3H3CO
CH3
H
H
H
OH
H3CO
OH
NH
OO
OH
COLCHICINEPODOFYLLOTOXIN COMBRETASTATIN
D-24851
NOCODAZOLE
DOLASTATIN VINBLASTINE
NOSTODIONE
2-METHOXY-ESTRADIOL
STABILIZERSNH
O
OH
O
OH
AcO O OH
O
AcOOO
H
OO
OH
H
OH
OH
OH O
O
NH2
+
+
Diaz et al J Biol Chem 2000
S
NO
O OH
OOHO
TAXOL
DISCODERMOLIDE
EPOTHILONES
NEW ANTI-TUB DRUGS
COMPANY NAME MOLECULE BINDING SITE CLINICAL TRIALS
Asta Medica AG D-24851 New Phase II
Astra Zeneca ZD-6186 Colchicine Phase II
Aventis AVE-8062 Colchicine Phase II
OxiGene Combretastatine 4P Colchicine Phase II
Abbott ABT-751 Colchicine Phase I
Wyeth HTI-286 Colchicine Phase I
EntrMed 2-Methoxyestradiol Colchicine Phase III
Aventis LIT-976 taxoid Taxol Phase III
Bayer Taxane Bay-59-8862 Taxol Phase III
BMS Taxanes BMS247550 Taxol Phase III
Wyeth Taxane Mac 321 Taxol Phase III
BMS Epothilones Taxol Phase III
KosanRoche Epothilone KOS862 Taxol Phase III
SheringMetagen Epothilones Taxol Phase III
Novartis Epothilone EPO-906 Taxol Phase III
TUBULIN DESTABILIZERS (7)
TUBULIN STABILIZERS (8)
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
TUBULIN - TARGET
MICROTUBULE
HETERODIMER
DESTABILIZING AGENTSINHIBIT POLYMERIZATION
DISASSEMBLE MICROTUBULES
STABILIZING AGENTSALTER MICROTUBULE
DYNAMICS
bull MITOTIC ARREST ANTI-PROLIFERATIVE ACTIVITYbull SHUTDOWN OF TUMOR BLOOD SUPPLY
DESTABSTRUCTURES
S
O
N
NH
NH OCH3
O
N
NHO
O
N
Cl
NH
N
OH
N
H3CO
O
H3CO N
OH
O
O
O OCH3
H
H3CO
H3CO
H3COOH
H3CO
S
N
NH
O
OCH3
NH
OH3CO
NHNH
O
O
N
H3CO
H3CO
H3CO
OCH3
O
NHCH3
O
O
O
O
O
OH
OCH3
OCH3H3CO
CH3
H
H
H
OH
H3CO
OH
NH
OO
OH
COLCHICINEPODOFYLLOTOXIN COMBRETASTATIN
D-24851
NOCODAZOLE
DOLASTATIN VINBLASTINE
NOSTODIONE
2-METHOXY-ESTRADIOL
STABILIZERSNH
O
OH
O
OH
AcO O OH
O
AcOOO
H
OO
OH
H
OH
OH
OH O
O
NH2
+
+
Diaz et al J Biol Chem 2000
S
NO
O OH
OOHO
TAXOL
DISCODERMOLIDE
EPOTHILONES
NEW ANTI-TUB DRUGS
COMPANY NAME MOLECULE BINDING SITE CLINICAL TRIALS
Asta Medica AG D-24851 New Phase II
Astra Zeneca ZD-6186 Colchicine Phase II
Aventis AVE-8062 Colchicine Phase II
OxiGene Combretastatine 4P Colchicine Phase II
Abbott ABT-751 Colchicine Phase I
Wyeth HTI-286 Colchicine Phase I
EntrMed 2-Methoxyestradiol Colchicine Phase III
Aventis LIT-976 taxoid Taxol Phase III
Bayer Taxane Bay-59-8862 Taxol Phase III
BMS Taxanes BMS247550 Taxol Phase III
Wyeth Taxane Mac 321 Taxol Phase III
BMS Epothilones Taxol Phase III
KosanRoche Epothilone KOS862 Taxol Phase III
SheringMetagen Epothilones Taxol Phase III
Novartis Epothilone EPO-906 Taxol Phase III
TUBULIN DESTABILIZERS (7)
TUBULIN STABILIZERS (8)
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
DESTABSTRUCTURES
S
O
N
NH
NH OCH3
O
N
NHO
O
N
Cl
NH
N
OH
N
H3CO
O
H3CO N
OH
O
O
O OCH3
H
H3CO
H3CO
H3COOH
H3CO
S
N
NH
O
OCH3
NH
OH3CO
NHNH
O
O
N
H3CO
H3CO
H3CO
OCH3
O
NHCH3
O
O
O
O
O
OH
OCH3
OCH3H3CO
CH3
H
H
H
OH
H3CO
OH
NH
OO
OH
COLCHICINEPODOFYLLOTOXIN COMBRETASTATIN
D-24851
NOCODAZOLE
DOLASTATIN VINBLASTINE
NOSTODIONE
2-METHOXY-ESTRADIOL
STABILIZERSNH
O
OH
O
OH
AcO O OH
O
AcOOO
H
OO
OH
H
OH
OH
OH O
O
NH2
+
+
Diaz et al J Biol Chem 2000
S
NO
O OH
OOHO
TAXOL
DISCODERMOLIDE
EPOTHILONES
NEW ANTI-TUB DRUGS
COMPANY NAME MOLECULE BINDING SITE CLINICAL TRIALS
Asta Medica AG D-24851 New Phase II
Astra Zeneca ZD-6186 Colchicine Phase II
Aventis AVE-8062 Colchicine Phase II
OxiGene Combretastatine 4P Colchicine Phase II
Abbott ABT-751 Colchicine Phase I
Wyeth HTI-286 Colchicine Phase I
EntrMed 2-Methoxyestradiol Colchicine Phase III
Aventis LIT-976 taxoid Taxol Phase III
Bayer Taxane Bay-59-8862 Taxol Phase III
BMS Taxanes BMS247550 Taxol Phase III
Wyeth Taxane Mac 321 Taxol Phase III
BMS Epothilones Taxol Phase III
KosanRoche Epothilone KOS862 Taxol Phase III
SheringMetagen Epothilones Taxol Phase III
Novartis Epothilone EPO-906 Taxol Phase III
TUBULIN DESTABILIZERS (7)
TUBULIN STABILIZERS (8)
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
STABILIZERSNH
O
OH
O
OH
AcO O OH
O
AcOOO
H
OO
OH
H
OH
OH
OH O
O
NH2
+
+
Diaz et al J Biol Chem 2000
S
NO
O OH
OOHO
TAXOL
DISCODERMOLIDE
EPOTHILONES
NEW ANTI-TUB DRUGS
COMPANY NAME MOLECULE BINDING SITE CLINICAL TRIALS
Asta Medica AG D-24851 New Phase II
Astra Zeneca ZD-6186 Colchicine Phase II
Aventis AVE-8062 Colchicine Phase II
OxiGene Combretastatine 4P Colchicine Phase II
Abbott ABT-751 Colchicine Phase I
Wyeth HTI-286 Colchicine Phase I
EntrMed 2-Methoxyestradiol Colchicine Phase III
Aventis LIT-976 taxoid Taxol Phase III
Bayer Taxane Bay-59-8862 Taxol Phase III
BMS Taxanes BMS247550 Taxol Phase III
Wyeth Taxane Mac 321 Taxol Phase III
BMS Epothilones Taxol Phase III
KosanRoche Epothilone KOS862 Taxol Phase III
SheringMetagen Epothilones Taxol Phase III
Novartis Epothilone EPO-906 Taxol Phase III
TUBULIN DESTABILIZERS (7)
TUBULIN STABILIZERS (8)
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
NEW ANTI-TUB DRUGS
COMPANY NAME MOLECULE BINDING SITE CLINICAL TRIALS
Asta Medica AG D-24851 New Phase II
Astra Zeneca ZD-6186 Colchicine Phase II
Aventis AVE-8062 Colchicine Phase II
OxiGene Combretastatine 4P Colchicine Phase II
Abbott ABT-751 Colchicine Phase I
Wyeth HTI-286 Colchicine Phase I
EntrMed 2-Methoxyestradiol Colchicine Phase III
Aventis LIT-976 taxoid Taxol Phase III
Bayer Taxane Bay-59-8862 Taxol Phase III
BMS Taxanes BMS247550 Taxol Phase III
Wyeth Taxane Mac 321 Taxol Phase III
BMS Epothilones Taxol Phase III
KosanRoche Epothilone KOS862 Taxol Phase III
SheringMetagen Epothilones Taxol Phase III
Novartis Epothilone EPO-906 Taxol Phase III
TUBULIN DESTABILIZERS (7)
TUBULIN STABILIZERS (8)
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
05 M KCl
SPAWNING amp FERT
Paracentrotus lividus
FERTILIZATION
~ 1 000 000 EGGSFEMALE
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
STAGES
PRISM EARLY PLUTEUS
ACTIVEFEEDING
CLEAVAGE MESENCHYMEBLASTULA
MID-PLUTEUS2-3 DAYS
450 - 500
LATE GASTRULA
~ 140
~ 115 FERTILIZED EGG
HATCHING
9-16 h
BLASTULA
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
0 10 20 30 40 50 60 70
Age h
0
1
2
3
4
5
6
7
8
9V relative units
SU_EMBR TUBULIN
Gong amp Brandhorst Dev Biol 1988 (Lytechinus pictus)
I
II
III
IV
HATCHING
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
TUBULIN SU amp VERT
HIGHLY CONSERVATIVE POLYPEPTIDE BACKBONE~ 450 АMINO ACIDS 2-17 VARIABILITY
VARIABLEDOMAIN
~ 15-17 АA
COOHNH2
CROSS-PRECIPITATION WITH ANTIBODIES TO VERTEBRATE BRAIN TUBULIN(CHICKEN RAT PIG)
FITC - LABELEDPIG BRAIN TUBULIN
INCLUDES INTOMITOTIC SPINDLE
YHamagushi et al J Cell Biol 1985 (Clypeaster japonicus)
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
EFFECTTUB DESTAB
COLCHICINENOCODAZOLEVINBLASTINE
DOLASTATIN 15D-24851
Control
6-11 h
1-30 h
TAXOL
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
EFFECTANTIPROLIFERTARGET - DNA
AZIDOTHYMIDINEАPIGENIN
HYDROXYUREAMITOXANTRONE
MITOMYCIN СRUBOXYL
4-PHENYLBUTYRIC ACID5-FLUOROURACYL
APHIDICOLIN5-AZACYTIDINEDOXORUBICIN
CHLORAMBUCIL
TARGET - CYTOSKELETON
ACRYLAMIDECYTOCHALASIN D
ABNORMALCLEAVAGE
NO SPINNING
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
EFFECTIVE CONC
EC minimum M
SEA URCHIN
EMBRYOS CLEAVAGE
ALTERATION
TUMOR CELL
CULTURES
CYTOTOXICITY
PURIFIED TUBULIN
POLYMERIZATION
VINBLASTINE 02 023 15
D-24851 001 0036 - 54 03
DOLASTATIN 15 0005 0003 - 0005 23
COLCHICINE 50 0005 - 08 2 - 8
2-METOXYESTRADIOL 02 008 - 18 19 - 47
NOCODAZOLE 0005 005 - 03 001
IC 50 M (LITERATURE DATA)
REAGENT
SEE TUBULIN DESTABILIZERS DATABASE ON PUBCHEM gtgtgt
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
SCREENING METHOD
aFERTILIZED EGG
CLEAVAGE ARREST
N
NH
O
ON
Cl
SWIMMINGBLASTULA
SPINNING
CLEAVAGE
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
TREATMENT PROCEDURE
EXPOSED STAGES
HATCHED BLASTULA
50 MControl 10 M
005 M02 M1 M
5 ml SUSPENSION IN SEA WATER 700-3000 EMBRYOSMLONE FEMALE EMBRYOS ~ 30 PLATES
9 h (20-23о)
16 h (14-17о)
FERTILIZED EGG
5 мM 100 M 10 M
STOCK SOLUTIONS (IN DMSO ETHANOL)
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
OGT-TEST 2005
LATE BLASTULA
CONTROL COLCHICINE NOCODAZOLE D-248513 NEW COMPOUNDS
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
EXPENSES
350 - 400 $IN VITRO TUBULIN POLYMERIZATION
2500 - 10 000 $IN VIVO TEST
~ 200 $SEA URCHIN EMBRYO TEST
750 $
CELL CULTURE TESTIC50 FACS D10
- SEA URCHIN EMBRYO - A PROMISING MODEL IN SCREENING FOR TUBULIN DESTABILIZING ACTIVITY
- MICROTUBULES
- MT-ROLES
- TUBULIN - TARGET
- DESTABSTRUCTURES
- STABILIZERS
- NEW ANTI-TUB DRUGS
- SPAWNING amp FERT
- STAGES
- SU_EMBR TUBULIN
- TUBULIN SU amp VERT
- EFFECTTUB DESTAB
- EFFECTANTIPROLIFER
- EFFECTIVE CONC
- SCREENING METHOD
- TREATMENT PROCEDURE
- OGT-TEST 2005
- EXPENSES
-
