Sartorius Laboratory catalogue Products for research and ...

SartoriusLaboratory catalogueProducts for researchand quality control

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Ultrapure Water Systems

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Shakers HomogenizersCentrifugesFermenters

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For every purpose the right filter.Sartorius offers a wide selectionof filter types, filter holders andready-to-use filtration units.There are certainly reasons foryou to use these products if youare engaged in analysis, research,quality control or production. But which are the right ones foryou?

The choice naturally depends onyour particular application. Areyou interested in the particles,cells, microoorganisms, colloidsor proteins, which are retainedon the filter surface? Do youneed a visibly clear, particle-free,sterile or pyrogen-free filtrate? Must cells be harvested, or pro-teins concentrated or purified? Or will you use the filter as asupport or a porous interface.

On the next pages you will find a brief introduction to six majorapplication areas (listed topright). Should you have questionson these, or require advice onother filtration or separationneeds, our highly capable fieldstaff, our product specialists orour application engineers inGoettingen are always at yourdisposal. Addresses, telephoneand telefax numbers are given onthe back page.

We wish you every success,Sartorius Biotechnology

Application areas. Product description.

5

New!31 Sterile, single-use fun-

nels and monitors forquicker and simplervacuum filtration ofsamples for colonycounts

19 Minisart high flowsyringe filter holderswith polyethersulfonemembranes

23 Vacuum filtration unitsfor a direct, safe sterilefiltration of cell culturesolutions

67 Vivascience leading inultrafiltration

75 Sartocon Slice Cross-flow filtration systemfor scaleable resultsfrom 1 to 100 litres

80 Polyethersulfonemembranes with poresizes from 0.1 µm to0.45 µm

66 Sartopore 2 Capsulesfaster pressure filtra-tion and broaderchemical compatibility

Major applications:Concentration | PurificationAir | Gas filtrationMicrobiological quality controlSample cleaningSterile filtration(Pages 9 to 13)

Product descriptions:Syringe filter hold-ers and vacuum filtraton units(Pages 15 to 37)

Filtration unitsand devices forpressure filtrationof liquids(Pages 39 to 57)

Ready to connectventing units, pres-sure holders, minicartridge housingsand mini cartridges(Pages 59 to 66)

Ultrafiltrationunits, concentra-tion, purificationand removal ofproteins. Systemsfor cell culture (Pages 67–75)

Membrane filtersand Nutrient PadSets for use inanalysis, researchAnd control (Pages 78–92)

Instruments for use in laborato-ries (Pages 93–106)

Chemical compatibility(Pages 113–115)

Sartobind Membrane Adsorbers(Pages 107–112)

Training seminars for the phar-maceutical industry and for thefood and beverage industry(Pages 116–117)

7

Contents

Samples for HPLCMembrane filters have estab-lished themselves as the filters ofchoice for particle removingpreparation of the small volumesamples used for HPLC. The mostused pore size for this applicationis 0.45 µm, with 0.2 µm beingpreferred when the sample con-tains very fine particles.

Ready to use Minisart RC andMinisart SRP syringe filtrationunits are available in both poresizes, and in 4 mm, 15 mm and26 mm diameters. The small4 mm units are packed for easeof removal from the box, aspictured below.

The combination of PTFE filterholders and PTFE membranefilters is particularly suitable forthe filtration of samples for theNMR spectrometry and also forthe filtration of extremelyhydrolysisprone or oxygensensitive samples (see page 17).

Water samplesLarger sample volumes arerequired for analytical methodsfor various substances in water.Particle removal using 0.45 µmmembrane filters can be seen inthe respective instructions, andusing 50 mm membrane filters inglass or stainless steel vacuumfilter holders. Difficult-to-filtersamples, such as sludge samples,have to be filtrated with pressure(see page 49).

Aqueous solutions and solventsA 47 mm pressure holder,page 47, allows particle removalfrom litre volumes of solutionsfor cell counters. The all-glassfilter holder plus 0.45 µm mem-brane filter, type 184, is perfectlysuitable for the particle removalfrom solvents for HPLC.

Colloid and protein removalAs shown in the guide below,smaller filters have to be insertedfor the removal of colloids, e.g.pore sizes of 0.1 µm or smallerfor samples in nephelometry.Proteins require ultrafilters,which tend to rapid blockage due to their fineness. The Centri-sart I centrifugal units avoid this blockage by filtering in theopposite direction to the cen-trifugal force. The easy accessi-bility of the filtrate makesCentrisart I units extremely prac-tical for the preparation ofclinical samples and for the lipo-some separations.

Minisart RC andMinisart SRPSyringe unitsPages 16–19

All-glass filterholderPage 27–29

Centrisart I andVivaspincentrifugal unitsPage 67–71

Membrane filterPages 78–89

Samples for LAL testsThe samples are normally cleansolutions free of ultrafilter block-ing substances. Ultrasart D20units are designed for theremoval of interfering, low-molecular substances from thesesamples by simple pressure filtra-tion (see page 76).

Sample cleaning. Particle and protein removal.

9

Applications

Direction ofultrafiltration

Particles etc.

Centrifugalforce

Sample

Ultrafilter

Ultrafiltrate

Overview: membrane filters and ultrafilters, separation ranges

Coarse particles Fine particles Colloids LiposomesYeasts | moulds Bacteria Viruses Pyrogens

Cells Proteins Peptides Salts

8 3 0.8 0.65 0.45 0.2 0.1 300,000 D 20,000 D 5,000 Dµm µm µm µm µm µm µm MWCO MWCO MWCO|————————–––––––––—– Membrane Filters ———–––––––——–––––—–|——––––—––––— Ultra Filters —–––––—–––—–|

Concentration and Purification of proteins.

The Sartorius separation tech-niques for biomolecules includeultrafiltration and membraneadsorption.

Vivaspin centrifugal units orVivacell pressure filtration unitsare commonly used for smallvolume concentration by ultra-filtration. Centrisart I, because ofits patented separation concept,is particularly advantageouswhen the ultrafiltrate is of interest.

Crossflow filtration has estab-lished itself as an economicalmean of processing medium tolarge volumes. Vivaflow units fillsperfectly the gap between thecentrifugal units and the cassettesystems. These ready-to-connectunits are a time- and cost-savingpossibility to treat samples in a laboratory standard and to giveat the same time forecasts con-cerning the scale-up to largervolumes.

All of the above products utilizeultrafiltration membranes whichseparate on the basis of size, and which exhibit low proteinadsorption characteristics.Membrane Adsorbers (membranefilters with built-in ion exchang-er groups) offer exciting newpossibilities for rapid isolationand purification.

New kits for protein purificationVivapure spin columns are cen-trifugal filter units with Sartoriusmembrane adsorbers for proteinpurification in biomedicalresearch and other biologicaldisciplines. The new Vivapure spincolumns are for the separation ofpure biological molecules fromcomplex mixtures and use thebasic principle of ion exchange.These centrifugal units are avail-able with strong and weak anionor cation charged membranematrixes.

With this new generation of ionexchange membranes proteinbinding, elution and consequentpurification and concentrationwill be much faster, more cost-effective, and eliminate addition-al steps associated with othermethodologies.

The main advantage of Vivapureis, that several samples can betreated simultaneously by usingsimple centrifugal units. Thismakes extensive isolatingendeavours with high samplefigures possible also in laborato-ries without expensive HPLC andFPLC with automatic samplehandling.

Please ask for more information!

Scale-Up. Which unit for which volume?Filtration unit Filter area Typical sample volume

Vivaspin 500 0.50 cm2 0.5 ml

Vivaspin 2 1.20 cm2 2.0 ml

Centrisart I 0.79 cm2 2.5 ml

Vivaspin 6 2.50 cm2 6 ml

Vivaspin 20 6.0 cm2 20 ml

Vivacell 70 20.0 cm2 70 ml

Vivacell 250 40.0 cm2 250 ml

Vivaflow 50 50.0 cm2 1 l

Vivaflow 200 200.0 cm2 5 l

Sartocon Slice 0.1 – 0.5 m2 50 l

10

Sterile venting and air |gas filtrationThe naturally hydrophobic PTFEmembranes are the filters ofchoice for these applications.They are heat-resistant and canbe repeatedly autoclaved orsteam-sterilized, whether theyare used as disc filters in stainlesssteel holders or ready-to-connectunits, or as pleated filters inCapsules and Mini cartridges.

Midisart 2000 units can beautoclaved. The larger packs ofthese units contain MemoryDiscs, which slip over the hosenipple connector and allow trackto be kept of the number of ster-ilizing cycles.

Midisart 2000 units are designedfor the sterile venting of smallfermenters and culture vessels(6–120 litres) as well as for theventing of filling vessels for ster-ile, distilled water and culturemedia, and for the sterilization ofair fed into small fermenters.

Minisart HY (page 60), has only afourth of the filtration area andis used for sterile venting offlasks and for air sterilization intube systems.

The 47 mm stainless-steel device,type 16254 (page 61) is designedfor the installation in lines. Itfeatures a valve on the inlet sidefor draining the condensation.The air flow rate corresponds tothis of Midisart 2000.

Higher flow rates require propor-tionately more filter area.

PTFE membranefilterPage 84

Sartofluor capsulesPage 62

Midisart 2000Page 60

Sartofluor MinicartridgesPage 64

Sartofluor Capsules are ready-to-connect units, includingvalves, for the sterile venting oftanks and vessels. They are indi-vidually pretested and integritytestable at any time. The Capsuleis available in a choice of threedifferent sizes, with the followingpleated filtration ares: 0.05 m3,0.1 m3 and 0.2 m3. Flow rates ofair are shown in the diagram onpage 62.

Sartofluor Mini cartridges arerecommended when the ventila-tion filter has to be sterilizedsimultaneously with the cor-responding vessel via in-linesteam-sterilization. They aremounted in one of the stainlesssteel housings described onpage 63.

Clean room monitoringThe effectiveness with which thegelatine membrane filters(page 104) can collect airbornemicroorganisms, and simultane-ously maintain their viability, isdemonstrated in the comparisonwith a slit sampler. The water-soluble gelatine membranes arealso becoming increasinglyimportant for the collection ofviruses.

Sterile venting. Air | Gas filtration. Clean room monitoring.

11

Applications

80

Minisart-HY Midisart 2000142 mm device + PTFE membrane filterSartofluor capsules 0 05 m2

Sterilizing filtration of aqueous solutions.

How ready-to-connect unitsfrom Sartorius fulfil yourrequirements

1. Cost-savingsThe combination of large filterarea and optimized geometry ofthe filter supports of Minisartsand Sartolab P20 units ensurehigh flow rates at low pressuresand optimal throughputs. Often,the filterable volume can even bedoubled by using Minisart-plus or Sartolab P20 plus units withintegrated prefilter. Sartobran150 and 300 and Sartobran PCapsules contain a pleateddouble membrane for maximumeconomy.

2. Reliability and TestabilityAll units comprise practice-proven cellulose acetate or poly-ethersulfone membrane filters,tested according to HIMA. Eachsingle unit is tested for housingand membrane integrity prior to packaging, and statisticallyvalid numbers of units from eachbatch are subjected to theBacteria Challenge Test. All typescan be integrity tested by theuser before and after filtration.

3. No change in the solutionAll materials pass the USP PlasticsTest classe VI. Sartorius celluloseacetate membrane filters haveproven particularly low adsorp-tion and assure minimal loss ofproteins and preservatives (seeresults on the right).

Which unit for which volume?Filtration unit Filter area Typical sample volume

Minisart 5.3 cm2 100 ml

Minisart-plus 5.3 cm2 200 ml

Sartolab P20 20 cm2 5 l

Sartolab P20 plus 20 cm2 + 10 l

Sartobran 150 150 cm2 ++ 25 l

Sartobran 300 300 cm2 ++ 50 l

Sartobran P up to 0.45 m2 ++ 200 l

+ = with integrated glass fibre prefilter++ = with heterogeneous double membrane

12

Standard Minisart and Minisart-plusSyringe unitsPage 18

Sartobran 150 and300 CapsulesPage 41

Sartolab P20 and Sartolab P20 plusunitsPage 40

Sartobran PCapsulesPage 42

Comparison results of the adsorption of various filter materials

0

10

20

30

BSA (µm/cm ) IgG (µm/cm )2 2

40

139.6

1 2 3 4 1 2 3 4

1 = Nylon 3 = PVDF2 = SFCA 4 = PES

Colony countsThe requirements for a practicalmicrobiological test method arethat it permits quantitative andreproducible detection of tracecontamination, and that it can be performed efficiently andeconomically under routineconditions. These requirementsare optimally fulfilled by themembrane filter method.

The sample is filtered through amembrane filter, which is thenrinsed, placed on a culture medi-um and incubated. The resultingcolonies are counted and relatedto the filtered volume.

Individually packed, griddedmembrane filters are manufac-tured specifically for this applica-tion. They are ready-for-use,quality controlled for colonygrowth and packed in easilyopened envelopes, each clearlymarked with product identifica-tion and lot number.

Nutrient Pad SetsPage 90, 91

Vacuum filtrationunitsPage 30, 31, 32

Individuallypacked, griddedmembrane filtersPage 88, 89

Gelatin membranefiltersPage 102, 103, 104

Nutrient Pads are a further sim-plification. They are culturemedia in dry form, sterile packedin Petri dishes, easy to stock andof consistent high quality, readyfor use after simply wetting withwater. Typical colonies grown onthe various types are shown onpage 91. The wide range of mediaincludes types for food, beverageand pharmaceutical industries.

Other water testings142 mm diameter polyamidemembranes of 0.2 µm or 0.45 µmpore size are specified in amethod for the collection oflegionella organisms (seepage 83).

A method using a SartoconCrossflow system with a 100,000cut-off polyethersulfone cas-settes for the recovery of bacte-riophages from water has shownvery good results.

Airborne bacteria and virusesGelatin membrane filters areroutinely used to quantitativelycollect airborne microorganismsfor clean room and isolatormonitoring. Their effectivenessfor the collecton of viruses hasalso been demonstrated. Thegelatin appears to have a protec-tive effect on the captured virus-es and can be dissolved in bufferor medium for subsequent virusdetection.

A recent publication describestheir use for the routine monitor-ing of bacteriophages in theambient air of milk processingfacilities.

For quicker and simpler samplefiltration

Sterile, single use Funnels andMonitors to replace stainless steelfunnels of vacuum holders.

Biosart 250® Funnels250 ml funnels (above left) whicheliminate the need for time-con-suming sterilization betweensamples. Their large inner basediameter ensures shortest filtra-tion times.

Biosart 100® Monitors100 ml capacity units (aboveright) with filters available indifferent pore sizes, filter coloursand diameters. The completelysterile units have to be used inconnection with various culturemedia. Lid and base form a Petridish after the culture mediumhas been added.

Further information on request.

Microbiological quality control.

13

Applications

100100

8080

6060

4040

2020

Funnel

Filter

Pad

Stopper

Lid New!

Syringe filter holdersand vacuum filtration units.

Minisart® RC units with hydrophilic, solvent-resistant RC-membranes.

Ready-to-use syringe filter unitsfor simple, rapid and reliableultracleaning of small-volumesamples for the HPLC or GCanalysis.

Minisart RC4 is recommended forsample volumes of up to about5 ml and Minisart RC25 forsample volumes of up to about100 ml.

Minisart RC units outperform competitive hydrophilic units interms of compatibility withaqueous solutions and solventmixtures.

They are compatible with thefollowing substances:Acetone HexaneAcetonitrile IsobutanolGasoline Isopropanoln-Butanol MethanolCellosolve (ethyl) Methylene-

chlorideChloroform Methyl-

ethylketoneDiethyl acetamide PentaneDimethyl sulfoxide Tetrahydro-

furanDioxane TolueneAcetic acid (96%) Trichloro-

aceticEthanol acid (25%)Ethyl acetate Trichlor-

ethaneEthylene glycol WaterFreon TF Xylene

Specifications for Minisart RC4, RC15 and RC25Connectors, Female luer lock inlet. Luer slip outletBubble point with water, > 2.0 bar (0.45 µm), > 3.4 bar (0.2 µm)

Flow rate for hexane at ∆p = 1 bar (100 kPa),a) Minisart RC4: 10 ml/min (0.45 µm), 3.5 ml/min (0.2 µm)b) Minisart RC15: 280 ml/min (0.45 µm), 140 ml/min (0.2 µm)c) Minisart RC25: 430 ml/min (0.45 µm), 230 ml/min (0.2 µm)

Flow rate for methanol at ∆p = 1 bar (100 kPa),a) Minisart RC4: 3.0 ml/min (0.45 µm), 1.5 ml/min (0.2 µm)b) Minisart RC15: 105 ml/min (0.45 µm), 55 ml/min (0.2 µm)c) Minisart RC25: 325 ml/min (0.45 µm), 160 ml/min (0.2 µm)

Flow rate for water at ∆p = 1 bar (100 kPa),a) Minisart RC4: 1.5 ml/min (0.45 µm), 0.5 ml/min (0.2 µm)/3 barb) Minisart RC15: 30 ml/min (0.45 µm), 10 ml/min (0.2 µm)c) Minisart RC25: 100 ml/min (0.45 µm), 60 ml/min (0.2 µm)

Filter diameter, 4 mm (RC4), 15 mm (RC15), 25 mm (RC25)Filter area, 0.07 cm2 (RC4), 1.7 cm2 (RC15), 4.8 cm2 (RC25)Filling volume, 0.17 ml (RC4), 0.2 ml (RC15), ca. 0.95 ml (RC25)

Housing burst pressure, 6 bar (600 kPa) and higherMaterials, polypropylene (housing), cellulose (membrane filter)Max. temperature, 121°C, 30 min (autoclave)Pore size, 0.45 µm or 0.2 µmHold-up volume, 5 µl (RC4), 10 µl (RC15), ca.150 µl (RC25)

Order Numbers for Minisart RC417821 K with 0.2 µm membrane, pack of 5017821 Q with 0.2 µm membrane, pack of 50017822 K with 0.45 µm membrane, pack of 5017822 Q with 0.45 µm membrane, pack of 500

Order Numbers for Minisart RC1517761 K with 0.2 µm membrane, pack of 5017761 ACK with 0.2 µm membrane, pack of 50, sterile,individually packed17761 Q with 0.2 µm membrane, pack of 50017762 K with 0.45 µm membrane, pack of 5017762 ACK with 0.45 µm membrane, pack of 50, sterile,individually packed17762 Q with 0.45 µm membrane, pack of 500

Order Numbers for Minisart RC2517764 K with 0.2 µm membrane, pack of 5017764 ACK with 0.2 µm membrane, pack of 50, sterile,individually packed17764 Q with 0.2 µm membrane, pack of 50017765 K with 0.45 µm membrane, pack of 5017765 ACK with 0.45 µmmembrane, pack of 50, sterile,individually packed17765 Q with 0.45 µm membrane, pack of 500

16

Ready-to-use units for simple,rapid and reliable ultracleaningof small-volume samples for theHPLC or GC analysis, whichrequire an even more chemicalresistant unit than Minisart RC,e.g. for solvents such as acetone,dimethylformamide and DMSO,or for aggressive aqueous liquids.

Minisart SRP4 is recommendedfor sample volumes of up toabout 1 ml, Minisart SRP15 forup to about 5 ml and MinisartSRP 25 for up to 100 ml.

Specifications for Minisart SRP4, SRP15 and SRP25Connectors, female luer lock inlet, luer slip outlet (Minisart-SRP15 is also available with a small spike outlet) Bubble point with isopropanol, 0.9 bar (0.45 µm) or 1.4 bar (0.2 µm)

Flow rate for ethanol at ∆p = 1 bar (100 kPa),a) Minisart SRP4: 2.0 ml/min (0.45 µm)/3 barb) Minisart SRP15: 45 ml/min (0.45 µm), 20 ml/min (0.2 µm)c) Minisart SRP25: 130 ml/min (0.45 µm), 70 ml/min (0.2 µm)

Flow rate for methanol at ∆p = 1 bar (100 kPa),a) Minisart SRP4: 4.5 ml/min (0.45 µm)b) Minisart SRP15: 150 ml/min (0.45 µm), 55 ml/min (0.2 µm)c) Minisart SRP25: 260 ml/min (0.45 µm), 160 ml/min (0.2 µm)

Flow rate for air at ∆p = 1 bar (100 kPa),a) Minisart SRP4: 0.06 l/min (0.45 µm)b) Minisart SRP15: 1.1 l/min (0.45 µm), 0.5 l/min (0.2 µm)c) Minisart SRP25: 1.8 l/min (0.45 µm), 1.2 l/min (0.2 µm)

Filter diameter, 4 mm (SRP4),15 mm (SRP15), 25 mm (SRP25)Filter area, 0.07 cm2 (SRP4), 1.7 cm2 (SRP15), 4.8 cm2 (SRP25)

Housing burst pressure, 6 bar (600 kPa) and higherMaterials, polypropylene (housing), polypropylene-reinforced PTFE(membrane filter)Max. temperature,121°C, 30 min (autoclave)Pore size, 0.45 µm or 0.2 µm (Minisart-SRP4, only 0.45 µm)Hold-up volume,1 µl (SRP4), 10 µl (SRP15),100 µl (SRP25)Water penetration point, 3.0 bar (0.45 µm) or 4.0 bar (0.2 µm)

Order Numbers for Minisart SRP417820 K with 0.45 µm membrane, pack of 5017820 Q with 0.45 µm membrane, pack of 500

Order Numbers for Minisart SRP15 with spike outlet17558 K with 0.2 µm membrane, pack of 5017558 Q with 0.2 µm membrane, pack of 50017559 K with 0.45 µm membrane, pack of 5017559 Q with 0.45 µm membrane, pack of 500

Order Numbers for Minisart SRP15 with luer outlet17573 K with 0.2 µm membrane, pack of 5017573 ACK with 0.2 µm membrane, pack of 50, sterile,individually packed17573 Q with 0.2 µm membrane, pack of 50017574 K with 0.45 µm membrane, pack of 5017574 Q with 0.45 µm membrane, pack of 500

Order Numbers for Minisart SRP2517575 K with 0.2 µm membrane, pack of 5017575 ACK with 0.2 µm membrane, pack of 50, sterile,individually packed17575 Q with 0.2 µm membrane, pack of 50017576 K with 0.45 µm membrane, pack of 5017576 Q with 0.45 µm membrane, pack of 500

Minisart® SRP units with a clean and chemically inert PTFE membrane.

17

Syringe filter holders

Minisart® 0.2 µm syringe filter holders for rapid small volume sterilization with maximum user comfort.

Ready-to-use units, which offerhigh flow rates at low inlet pres-sures, make an correspondinglyrapid sterile filtration possible.Fitted on a standard syringe, theyenable a less hand-tiring sterili-zation of up to 100 ml of liquid.A Minisart fitted on a standarddosing syringe makes up a veryconvenient system for simultane-ous dosing and sterilization.

The combination of a large filtra-tion area and a optimised geo-metry of the filter support, whichare responsible for the high flowrates, also ensures high totalthroughputs.

Minisart plus units are of advan-tage for the sterilization ofdifficult to filter liquids. Theyinclude a fine glass fibre prefilteron the filter membrane, a combi-nation which is so effective that throughputs can often bedoubled. Minisarts and theirpackaging are environmentallyfriendly, free of PVC!

Minisart High flow are syringefilter holders with a polyether-sulfone membrane for the sterilefiltration at higher flow rates and a higher filtration speed.

Specifications for 0.2 µm Minisarts and Minisart plusAdsorption Values determined for the cellulose acetate

membrane, 0.8–3 µg/cm2 with RSA, 8–12 µg/cm2

with gamma globulin.Bubble point with water, minimum value 3.4 bar (340 kPa)Colour coding blueConnectors Female luer lock inlet, male luer lock outlet.

Alternatively only for standard Minisarts male luer lock outlet

Cytotoxicity No inhibition with MRC-5 or L-929 cellsEndotoxins Endotoxin-output below the detection limit of

the tests (0.06 EU/ml)Filter diameter 26 mmFilter area 5.3 cm2

Flow rate Typical values for water at p = 1 bar (100 kPa)60 ml/min

Hold-up volume 0.1 ml for standard Minisarts, 0.23 ml for Minisart plus

Application Max. recommended operational pressure, 4.5 bar (450 kPa) limits housing burst pressure, 6 bar (600 kPa) and higherMax. temperature, 50°C

Materials Cellulose acetate membrane filters, glass fibreprefilters (only for Minisart plus), MBSpolymerisate

Order Numbers for 0.2 µm Minisarta) pack of 50, sterile, individually packed:

16534 K with luer lock outlet17597 K with male luer lock outlet

b) pack of 500, not sterile bulk packed:16534 Q with luer lock outlet17597 Q with male luer slip outlet

Order Numbers for 0.2 µm Minisart plusa) pack of 50,sterile, individually packed:

17823 K with luer lock outlet

b) pack of 500, not sterile bulk packed:17823 Q with luer lock outlet

Order Numbers for Minisart high flowa) 0.1 µm, pack of 50, sterile, individually packed:

16553 K with luer lock outlet

b) 0.2 µm, pack of 50, sterile, individually packed:16532 K with luer lock outlet16541 K with luer slip outlet

c) 0.45 µm, pack of 50, sterile, individually packed:16533 K with luer lock outlet16537 K with luer slip outlet

18

Ready-to-use filter units with0.45 µm, 0.8 µm,1.2 µm or 5 µmpore size membrane filters. Theseindependent filters fulfill yourfiltration requirements whenevervolumes of up to 100 ml must beclarified or ultracleaned. Theycan also be used as prefilter incombination with a 0.2 µmMinisart, increasing the totalfilterable volume.

The high flow rates of these unitsresult from the large filter areaand the very low flow resistanceof the filter support, which isdemonstrated by the relativeconstant increase in the flow ratewith increasing pore size.

These flow rates contribute touser comfort by lowering thepressure required for filtration.Minisart GF contains a glass fibrefilter with a retention efficiencyof 98% for 0.7 µm spherical par-ticles. It is very useful when rela-tively dirty solutions are to beclarified, or as a prefilter on an0.2 µm or 0.45 µm Minisart.Minisart plus units also containthis glass fibre filter, but as pre-filter on an 0.45 µm or 1.2 µmmembrane, for higher totalthroughputs.

Specifications for Minisarts, Minisart plus and Minisart GFConnectors Female luer lock inlet, male luer lock outlet

(the 0.45 µm unit is also available with a maleluer lock outlet)

Application limits Max. recommended operating pressure 4.5 bar(450 kPa).Housing burst pressure 6 bar (600 kPa) and higherMax. temperature 50°C

Bubble point Min. value with water, 2.0 bar (0.45 µm) 0.8 bar(0.8 µm), 0.7 bar (1.2 µm), 0.4 bar (5 µm), 0.5 bar(Minisart-GF)

Flow rate Typical values for water at ∆ = 1 bar (100 kPa),160 ml/min (0.45 µm), 350 ml/min (0.8 µm), 400 ml/min (1.2 µm), 500 ml/min (5 µm), 450 ml/min (Minisart-GF)

Colour coding yellow (0.45 µm), green (0.8 µm), red (1.2 µm), brown (5 µm), opaque (Minisart GF)

Filter diameter 26 mmFilter area 5.3 cm2

Materials Cellulose acetate membrane (except Minisart GF). Glass fibre filter (Minisart GF and Minisart plus). MBS polymerisate).

Hold-up volume 0.1 mlCytotoxicity Detectably no inhibitoin with MRC-5 (human

lung cells)

Order Numbers for standard 0.45 µm to 5 µm Minisartsa) pack of 50, sterile, individually packed:

17598 K 0.45 µm Minisart with male luer lock outlet16555 K 0.45 µm Minisart with luer lock outlet16592 K 0.8 µm Minisart with luer lock outlet17593 K 1.2 µm Minisart with luer lock outlet17594 K 5 µm Minisart with luer lock outlet

b) pack of 500, not sterile bulk packed:17598 Q 0.45 µm Minisart with male luer lock outlet16555 Q 0.45 µm Minisart with luer lock outlet16592 Q 0.8 µm Minisart with luer lock outlet17593 Q 1.2 µm Minisart with luer lock outlet17594 Q 5 µm Minisart with luer lock outlet

Order Numbers for Minisart plus unitsa) pack of 50, sterile, individually packed:

17829 K 0.45 µm with luer lock outlet

b) pack of 500, not sterile bulk packed:17829 Q 0.45 µm with luer lock outlet17825 Q 1.2 µm with luer lock outlet

Order Numbers for Minisart GF unitsnot sterile bulk packed: 17824 K luer lock outlet (pack of 50)17824 Q luer lock outlet (pack of 500)

Recommended accessories see page 22

High flow rate Minisart® Syringe filters for particle removal, ultracleaning and prefiltration.

19


200

300

400400400400400400

Re-usable, 13 mm Syringe Filter Holders for the ultracleaning of small volumes (up to about 10 ml).

PTFE Holder for solvents and chemicalsMade completely of PTFE, thisholder is unaffected by chemicalsand contains no trace elementswhich could be released into theliquid being filtered. It is there-fore extremely well suited forparticle removal from samplesand reagents for analyticalmethods, such as NMR samples.Another benefit of this applica-tion is the low hold-up volume,the easy cleaning and the dryingat a temperature of 180°C.

The construction of the holderensures leak proof sealing with-out a sealing ring, and avoidstwisting of the membrane filterwhen the top is tightened ontothe base.

Polycarbonate Holder for aqueous solutions This inexpensive filter holder ismade of clear, autoclavable poly-carbonate and contains a siliconegasket for leak proof sealing. Itcan be used at pressures of up to7 bar by simple, manual screwingtogether.

Filter supports in the top andbottom parts allow filtration ineither direction.

Specifications for the 13 mm PTFE syringe filter holderConnectors, female luer lock inlet, luer slip outletChemical compatibility, as for PTFEFlow rate for water at ∆p = 1 bar (100 kPa),a) with 0.2 µm membrane filter, ca. 10 ml/minb) with 0.45 µm membrane filter, 18 ml/minFiltration area 0.5 cm2

Weight 13 gMaterials PTFE top and bottom partMax. operating pressure 5 bar (500 kPa)Membrane filter diameter 13 mmSterilization by autoclaving (max. 134°C) or by dry heat

(max. 180°C)Hold-up volume less than 0.03 ml after overcoming the

bubble point (0.3 ml before)

Order number for the 13 mm PTFE filter holder: 16574

Specifications for the 13 mm polycarbonate syringe filter holderConnectors, female luer lock inlet, luer slip outletChemical compatibility, as for polycarbonate and siliconeFlow rate for water at ∆p = 1 bar (100 kPa), ca. 18 ml/min with 0.2 µmMembrane filter 35 ml/min with 0.45 µm membrane filterFiltration area 0.5 cm2

Materials polycarbonate top and bottom part, silicone gasket 10 + 14.9 mm(replacement part no. 6980569 for a packof 10)

Max. operating pressure 7 bar (700 kPa)Membrane filter diameter 13 mmSterilization by autoclaving at 121°CHold-up volume less than 0.2 ml after overcoming the


Order number for the 13 mm polycarbonate syringefilter holder:16514E (pack of 12)

Recommended accessories are described on page 22.

20

Stainless Steel Holder forsolvents and chemicalsThe PTFE-coated surface on thetop part is an important propertyof the filter holder and ensuresleak proof sealing without a seal-ing ring. As a result, the heat-resistance is extremely good, andthe chemical compatibilitydepends only on the insertedfilter type.

The top part can easily be mount-ed on the bottom part using theenclosed tightening tool.

Filter supports in the top andbottom bottom parts allow filtra-tion in either direction.

Polycarbonate Holder foraqueous solutionsThis inexpensive filter holder ismade of clear, autoclavable poly-carbonate and offers a filtrationarea six times the amount of thatof the 13 mm holder describedon page 20. The silicone gasketenables a leak free filtration atpressures of up to 7 bar by sim-ple, manual screwing together.

Filter supports in the top andbottom parts allow filtration ineither direction.

Specifications for the 25 mm Stainless Steel HolderConnectors, female luer lock inlet, luer slip outletChemical compatibility, as for stainless steel and PTFEFlow rate for water at ∆p = 1 bar (100 kPa), ca. 45 ml/min with 0.2 µmMembrane filter, 80 ml/min with 0.45 µm membrane filterFiltration area 3 cm2

Materials stainless steel (1.4305) top and bottomparts. PTFE-coated sealing area in top part.Luran 368R tightening tool (replacementpart no. 6980595)

Max. operating pressure 7 bar (700 kPa)Membrane filter diameter 25 mmSterilization by autoclaving (max. 134°C) or by dry heat

(max. 180°C)Hold-up volume less than 0.1 ml after overcoming the


Order number for the 25 mm stainless steel holder:16214


Specifications for the 25 mm Polycarbonate syringe filter holderConnectors, female luer lock inlet, luer slip outletChemical compatibility, as for polycarbonate and siliconeFlow rate for water at ∆p = 1 bar (100 kPa), ca. 70 ml/min with 0.2 µmmembrane filter, 110 ml/min with 0.45 µm membrane filterFiltration area 3 cm2

Materials polycarbonate top and bottom parts, silicone flat gasket 20.5 + 26.5 mm(replacement part no. 6980570 for a packof 10)

Max. operating pressure 7 bar (700 kPa)Membrane filter diameter 25 mmSterilization by autoclaving at 121°CHold-up volume less than 0.3 ml after overcoming the


Order number for the 25 mm polycarbonate syringefilter holder: 16517E (pack of 12)


Re-usable 25 mm Syringe Filter Holders for the ultracleaning and sterilizing filtration of volumes of up to about 100 ml.

21


Accessories for ready-to-use Minisarts and re-usable Syringe Filter Holders.

2. Descriptions of Accessories

2a. Dosing Syringe Order number: 16685-2 The dosing syringe is perfectlysuitable in connection with afilter holder for a rapid and sim-ple filtration. The new dosingsyringe in combination with ourMinisart filter holders are idealfor the wetting of our nutientpad sets with sterile water.

The volume of the dosing syringecan be infinitely adjusted from0.5 to 5.0 ml by turning the screwat the handle. The syringe is user-and maintenance-friendly. More-over, it is very easy to handle andavoids so fatigue signs of thehand after longer use.

The dosing syringe can bedisinfected by boiling out. It isnot recommended to autoclavethe syringes. If it is exceptionallynecessary, the plastic handle hasto be removed first.

2b. 3-way valve Order number: 16639Allows continuous filtration to becarried out, connected to asyringe and fitted on the outletside with a filter holder.

Autoclavable (121°C).Replacement parts:6986070 Sealing (4+)6986071 Pressure spring (2+)6986072 Fixing spring (2+)6986073 Perbunan valve (2+)

2c. Disposable syringes Order numbers: 16644E 5 ml volume, pack of 1216645E 10 ml volume, pack of 1216646E 20 ml volume, pack of 1216647E 50 ml volume, pack of 12

They can be used with the 3-wayvalve and the filter holders withfemale luer lock inlet connection.One packet contains 12 individu-ally packed needles and 12 dis-posable needles.

2d. Needles Order numbers: 01324 Stainless steel needle 01325 Disposable needleFit on the luer slip outlets of thesyringe filter holders. Allow sili-cone caps or rubber bungs to bepierced and the filtrate to beinduced selectively into a tube oran other vessel. The stainless steelneedle is autoclavable.

2e. Minicheck BPOrder number: 17799Pressure gauge for the determi-nation of the bubble point ofMinisart syringe filter holderswith luer lock connectors. For thedetermination of the bubblepoint of Sartolab P20 or SartolabP20 plus units, the integrity testholder 18099 is required.

22

54

Sartolab RF and BT.New possibilities in vacuum filtration.

Sartorius SartolabVacuum filtration units.

Sartorius RF and BT units areoptimised for the application incell culture. The built-in mem-brane made of polyethersulfoneguarantees extremely high flowrates and low protein binding,and is therefore ideal for thefiltration of protein containingsolutions.

The receiver flask is deliveredwith tube adapter and closure lid.

The Sartolab RF units are sterilecomplete units with drainagevessel, the Sartolab BT holderscan be adapted on usual in trade,vacuum resistant bottles with ascrew connector 45. Attention:Only use bottles which arelicensed for subpressure methods.

Available in different sizes.

Sartorius Sartolab 150 VFiltration units with pleated0.2 µm PES membrane forvacuum filtration/ sterile filtra-tion of up to several litres.

By opening the drain valve pro-tected by a 0.2 µm PTFE mem-brane, the created vacuum canbe interrupted for replacing thefilled receiver flask through anew one. Filtration restarts, whenthe drain valve is getting closed.This procedure of the “continu-ous” filtration can be repeatedseveral times.

The 0.2 µm pleated PES mem-brane with an area of 150 cm2

guarantees a save sterile filtra-tion of media, buffer and a lot ofother solutions. They can be uni-versally applied for bottles with a diameter of up to 58 mm.

Order numbers for Sartolab RF Vacuum filtration units18081-E volume 150 ml, incl. receiver flask, pack of 1218082-E volume 500 ml, incl. receiver flask, pack of 1218083-E volume 1000 ml, incl. receiver flask, pack of 12

Order numbers for Sartolab BT Vacuum filtration units18084-E volume 150 ml, pack of 1218085-E volume 500 ml, pack of 1218086-E volume 1000 ml, pack of 12

Order numbers for Sartolab 150 V18080-M Sterile flask holders, pack of 3

The illustration shows the flow rate of the Sartorius PES membranewith RPMI cell culture media with and without additional FCScompared with polyethersulfone membranes of other manufacturers.

24

13

12

11

10

9

8

7

6

PES I PES II PES Sartorius

RPMI-1640 RPMI + 20%FCS

flow

spe

ed [

ml/c

m ·

min

]22

Type 16510 is complete withreceiver flask, and can be operat-ed with sub-pressure as well aswith slight over-pressure (0.5 baris recommended for higheststanding times). It is, togetherwith a vacuum hand pump, apractical, cost-effective systemfor the filtration in the labora-tory or outside.

For sterile filtrations, the filterholder, comprised in the delivery,is equipped with a glass fibrefilter 13400-0013 and enables asterile venting for pressure com-pensation in order to avoid con-tamination of the sterile filtrate.The funnel fits onto the centralopening of the lid and simplifiesthe pouring-in of liquid in thetop part.

Type 16511 is like 16510, butwithout receiver flask. It is usedon a suction flask or a vacuummanifold, e.g. Combisart,page 32.

Specifications for 47 mm polycarbonat holdersParts supplied type 16510, top part complete with lid,

stopper for lid, plug and funnel, base partwith hose nipple and filter holder, receiver flask with lid, all made of poly-carbonate: silicone O-rings for lid(80+3 mm), filter support (40+5 mm) andopening (14+2 mm). Polypropylene filtersupport

Components type 16511, like 16510 but withoutreceiver flask

Chemical compatibility as for polycarbonate, polypropylene and silicone

Flow rate for water at 90% vacuum, 200 ml/minwith 0.2 µm, 700 ml/min with 0.45 µm,2 l/min with 0.8 µm membrane filter

Capacity top part and receiver flask, 250 ml Filtration area 12.5 cm2

Max. operating pressure vacuum or max. 2 bar (200 kPa) over-pressure Suitable membrane filter dia-meter, 47 mm (prefilter, 37 mm)

Sterilization by autoclaving at 121°C. The polycarbon-ate material withstands numerous cycles,provided aggressive cleaning agents arecompletely washed off and that the steamdoes not contain anticorrosive, anti-scal-ing boiler water additives.

Order Numbers for 47 mm polycarbonate holders16510 Polycarbonate holder for 47 mm mem-

brane filter, with 250 ml top part andreceiver flask, for vacuum or pressurefiltration

16511 Polycarbonate holder for 47 mm mem-brane filter, with 250 ml top part, forvacuum filtration

Recommended Accessories are described under 1d/1e on page 33.

Polycarbonate Holders for the clarification or sterile filtration of up to about 200 ml volumes of aqueous solutions.

25

Vacuum filtration units

Replacement Parts16514E 13 mm filter holder

(pack of 12)6980110 Silicone O-ring,

40+5 mm6980225 Plug (pack of 10)6980226 Funnel6980227 Stopper for lid6980228 Lid6980229 Silicone O-ring,

80+3 mm (pack of 2)6980230 Top part, 250 ml6980232 Filter support

(pack of 2)

6980233 Base part6980234 Hose nipple6980235 Silicone O-ring,

14+2 mm (pack of 3)6980236 Silicone cap

(pack of 10)6981090 Receiver flask

School-Kit for microbiological experiments.

Complete KitsFor specific applications inmicrobiological testing, werecommend our practical,complete kit.

The school kit for microbiologicalexperiments is an ideal teachingaid for instruction in microbiolo-gy and environmental protectionin schools and other educationalinstitutes. The rugged aluminumcase contains the entire equip-ment for microbiological testing.The handbook included in thecase provides general instructionsand detailed descriptions ofmethods for 7 experiments:detection of microorganisms inwater, in air, in soil; the effects of antibiotics; detection of yeastson substrates in nature; produc-tion of gas through alcoholic fer-mentation; and bacterial growthat different temperatures.

The School-Kit for the science subjects.A complete equipment in astable-lockable aluminium casefor performing microbiologicalresearch. The brochure suppliedwith the kit contains a generalintroduction and a detaileddescription of the methods forseven experiments (detection ofmicroorganisms in water, air andsoil; effects of antibiotics; detec-tion of yeasts in natural sources;gas formation during alcoholicfermentation; growth of bacteriaat different temperatures).

The vacuum, which is necessaryfor the filtration, is created withhelp of a syringe and a 3-wayvalve.

Parts SuppliedAluminium caseStainless steel forceps (16625)Filtration system for samples(device 16510. 3-way valve16639. adapter 17108D. syringe 16647. glass fibre filter 13400-013S)Filtration system for sterile water(filter holder 16517E.syringe 16647. membrane filter11307-025N)Inoculation loop (17109)Culture media (nutrient broth)14132K. Endo nutrient pad sets,14053. Wort nutrient padsets14058.Standard nutrient pad sets,14055)

Order Number24002 School-Kit for microbio-

logical experiments, in alockable aluminium case

26

25 mm Glass Holder for hybridisation tests, particle testing and clarificationThe two devices differ only in thefilter support, the glass frit or thePTFE-coated screen support. Thedevice with glass frit ensuresuniform distribution of particlesand is therefore recommended,when the retained particles onthe filter surface are of interest.The device with a PTFE-coatedscreen support is, as it is easy toclean, more suitable, when thefiltrate is required, or for radio-chemical work.

The PTFE ring which holds theglass frit or the screen supportallows autoclaving the deviceswith a filter in position, withoutstickage of the filter to the sup-port, and also protects the rim ofthe glass frit from breakage andfrom potential leakage.

It has a rim around the upperedge, which simplifies the posi-tioning of the membrane filterwhen inserted, and a silicone ringon the underside for a reliableseal on the filtrate side. The fun-nel-shaped top part simplifiesfilling in the sample.

SpecificationsOutlet spout, 12 mm dParts and materials, borosilicateglass funnel and base.PTFE/glass filter support (type16306) and PTFE/stainless steel,coated with Teflon (type 16315).Silicone O-ring, 25 + 3 mm. Aluminium clamp.Chemical compatibility, as forglass, PTFE and silicone.The silicone O-ring can bereplaced by a Viton O-ring (orderno. 00118)

Flow rate for water at 90%vacuum, 50 ml/min with 0.2 µm,150 ml/min with 0.45 µm,500 ml/min with 0.8 µmmembrane filterFunnel capacity, 30 ml

Filtration area, 3 cm2

Suitable membrane filterdiameter, 25 mm (or 24 mm)Sterilization, by autoclaving(max. 134°C) or by dry heat(max. 180°C)

Order numbers16306 Glass device for 25 mm

membrane filter, withglass frit

16315 Glass device for 25 mmmembrane filter, with PTFE-coated screensupport

Recommended accessories are described under 1a on page 33

Replacement parts see diagram

25 mm Glass Holder for the filtration of small volumes.

27


6980115glas funnel 30 ml

6983008small clamp

17149screen support and PTEE-ring

17148glass frit and PTEE-ring

6980117siliconeO-ring

6980116glass base

50 mm Glass Holder with protective PTFE ring, for particle testing or clarification and sterile filtration.

This filter holder is available intwo versions differing from eachother only in the type of thefilter support. The filter withglass frit ensures uniform distri-bution of retained particles and istherefore recommended, whenthe residue on the filter surface isof interest. The device with PTFE-coated screen support, on theother hand, is due to its ease ofclean more preferable, when thefiltrate is required or when liq-uids difficult to remove fromglass frits have to be examined.

The PTFE ring which holds theglass frit and the screen supportallows autoclaving the deviceswith a filter in position, and pro-tects the edge of the glass fritfrom breakage and potentialleakage. It has a rim around theupper edge to simplify the posi-tioning of the membrane filterwhen inserted and a silicone O-ring in the underside for leak-proof seal on the filtrate side.

Specifications for the 50 mm glass holderOutlet spouts 15 mm outside diameterParts and materials borosilicate glass funnel and base.

Anodised aluminium clamp. Siliconecaoutchouc lid. PTFE/glass filter support(type 16307) and PTFE/stainless steel filtersupport, coated with Teflon (type 16316).

Silicone O-ring 45 + 3 mmChemical compatibility as for glass, PTFE and silicone (see

page 109). If required, the silicone O-ringcan be replaced by a Viton O-ring (orderno. 00124).

Flow rate for water at 90% vacuum, 200 ml/minwith 0.2 µm, 600 ml/min with 0.45 µm,2.2 l/min with 0.8 µm membrane filter

Funnel capacity 250 mlFiltration area, 12.5 cm2

Max. operating pressure only for subpressureSuitable membrane filter diameter 50 mm (or 47 mm)Sterilization by autoclaving (121°C or 134°C)

or by dry heat (max. 180°C)

Order number for the 50 mm glass holders16307 Glass vacuum filtration holder for 50 mm (or 47 mm)

membrane filter, with glass frit filter support16316 Glass vacuum filtration holder for 50 mm (or 47 mm)

membrane filter, with PTFE-coated screen filter support

Recommended accessories are described under 1b on page 33


28

6980121lid

6980119glass funnel, 250 ml

6983009large clamp

6980120glass base,50 mm

17146glass frit and PTFE ring with centre edge

6983006glass frit

17147screen support andPTFE ring with centre edge

6983010drain screen

6980123silicone O-ring

All areas, where liquid and devicecan get directly in contact, aremade of glass or PTFE. The device,in combination with solvent-resistant, hydrophilic RC-mem-branes (type 184, page 84), istherefore ideal for ultracleaningand degassing solvents andsolvent mixtures for HPLC, GCand AA.

Convenience of handling isensured by several beneficial fea-tures. A 6 mm wide non-groundrim above the ground glass neckof the suction flask prevents thefiltrate from contacting greaseon the ground glass surface andso avoids it becoming contami-nated while being poured out ofthe flask.

The hose nipple connector ismade of polypropylene for safeconnection of vacuum hose. Thefiltrate outlet spout ends wellbelow the entrance to this hosenipple.

Specifications for the all-glass holderParts and materials borosilicate glass funnel, base and flask.Filter support PTFE ring holding a glass frit, with Viton

O-ring(45 + 3 mm). Anodized aluminiumclamp.

Chemical compatibility as for glass and PTFEFlow rate for water at 90% vacuum, 200 ml/min

with 0.2 µm, 600 ml/min with 0.45 µm,2.2 l/min with 0.8 µm membrane filter

Funnel capacity 250 mlCapacity of the filtrate flask 1 litreFiltration area 12.5 cm2

Max. operating pressure only for subpressure Suitable membrane filter diameter, 50 mm(or 47 mm)

Sterilization (without connector) by autoclaving (121°C or 134°C)

or dry heat (max. 180°C)

Order number for the 50 mm glass holder16309 All-glass vacuum filtration unit for 50 mm (or 47 mm)

membrane filter, with vacuum-resistant flask, capacity 1 litre

Recommended accessories are described under 1c on page 33


All-glass holder for particle removal from solvents for analytical determinations.

29


6980119glass funnel

6983004filter support with glass frit and O-ring

6983001vauumresistant glass flask

6983002glass base

6983005Viton sealing for connector

6983003connector with sealing

00124Viton O-ring

6983009large clamp

Stainless steel holders with vacuum control for colony counts and particle collection.

The three holder types differ onlyin the funnel capacity (either40 ml, 100 ml or 500 ml). Theyhave been designed specificallyfor applications in which the par-ticles or microorganisms retainedon the membrane filter surfaceare of interest. The stainless steelfrit filter support ensures a uni-form distribution of the residues.

Regarding routine examinations,a simple handling is very impor-tant. Stainless steel taps in thebase part allowing turning on oroff the vacuum and the specialclosure clamps, which simplifyputting on or removing the fun-nels, enable this easy handling.

Specifications for the 50 mm stainless steel filter holdersOutlet spouts 10 mm outside diameterParts and materials lid (16220 without), funnel, base part,

filter support, clamp and tap made ofstainless steel. Silicone flat gasket. Silicone lid seal

Chemical compatibility as for stainless steel and PTFEFlow rate for water at 90% vacuum, 200 ml/min

with 0.2 µm, 600 ml/min with 0.45 µmmembrane filter

Filtration area 12.5 cmMax. operating pressure only for subpressureSuitable membrane filter diameter 50 mm/47 mmSterilization by autoclaving (max. 134°C), by dry heat

(max. 180°C) or by flaming

Order numbers for the 50 mm stainless steel filter holders16201 Stainless steel vacuum filtration unit for 50 mm membrane

filter, with 500 ml funnel capacity16219 Stainless steel vacuum filtration unit for 50 mm membrane

filter, with 100 ml funnel capacity16220 Stainless steel vacuum filtration unit for 50 mm membrane

filter, with 40 ml funnel capacity

Recommended accessories are described under 1 g on page 33

Replacement parts are shown in the diagram

30

6981063lid

6981064silicone lid seal

6981065funnel with clamp

6980102stainless steel frit

6980124silicone flat gasket

6982119spare parts(leaf spring,disk, safetydisk)

6980101base

6980101base

6982119spare parts(leaf spring, disk,safety disk)


6980102 stainless steel frit


6981001lid

6981003silicone lid seal


The Sartorius CombiSart systemenables you to select the optimalhardware and consumables foryour needs in quality assurance.CombiSart features a modulardesign and field-proven standardaccessories to make your choiceeasier.

At the heart of the CombiSartsystem is a stainless steel mani-fold designed to accommodateall types of filter holders andfunnels such as:

• Ready-to-use units, includingBioSart 100 Monitors andBioSart 250 Funnels

• Flamable units such as stainlesssteel funnels for colonycounting

• Autoclavable polycarbonateand glass filter holders

If required, we can send youbrochures with a detaileddescription of the Combisart sys-tems and the according nutrientmedia for the Biosart 100 moni-tors in the Combisart brochure.Biosart 100 monitors andBiosart 250 funnels can be com-bined with stainless steel single-and multi-manifolds.

CombiSart Multi-branch System.

31


Special brochure available on request.

BioSart® 100 Monitor, 100 ml.For colony counting.

Order Order information: number:0.2 µm*, white | black 16401-47-07-ACK

0.45 µm*, 16401-47-06-Kwhite | black 16401-47-06-ACK

0.45 µm*, green | 16402-47-06-Kdark green 16402-47-06-ACK

0.45 µm*, 16403-47-06-Kgrey | white 16403-47-06-ACK

* Diameter = 47 mm

BioSart® 250 Funnel, 250 ml.For colony and particle counting.

Order Order information: number:250 ml, pack of 50 16407-25-ALK250 ml,pack of 50 16407-25-ACKsingle packed

Nutrient Media Broth

Order information & Order numberTotal Count Broth16400-02-TC-KCaso (acc. to USP) Broth16400-02-CA-KCetrimide (acc. to USP) Broth16400-02-CE-KRZA Broth16400-02-RA-KOrange Serum Broth16400-02-OS-KSabouraud Broth16400-02-SB-KM-Endo Broth16400-02-EN-KM-Green Broth16400-02-MG-KM-FC Broth16400-02-MF-KKF Streph. Broth16400-02-KF-K

Order numbers16842 3-branch manifold16843 6-branch manifold16840 Single base for stainless

and disposable funnel16835 adapter base for Biosart16836, adapter for 16837 glass holders

3- and 6-Branch Manifold Systems for routine colony and particle counting.

The manifold systems are avail-able with 100 ml or 500 mlcapacity funnels. The three or sixseparate filtration holders allowtime-saving when mass examina-tions of 100 ml volulme sampleshave to be carried out. Due to thestainless steel taps on the mani-fold ports, the subpressure foreach holder can be turned on andoff individually.

The stainless steel filter supportallows a homogenous distribu-tion of the residues on the mem-brane filter surface. Funnel andfilter support can be disinfectedby flaming.

Specifications for 3- and 6-branch manifold systemsCompatibility Chemical resistance as for stainless steel

and silicone, see page 109Filtration area 12.5 cm2

Flow rate Typical values for water at 90% vacuum,600 ml/min with 0.45 µm, 200 ml/minwith 0.2 µm filter pore size

Parts and materials Stainless steel manifold, funnel, lid, frits.Silicone flat gasket. Silicone lid seal, capand hose connector

Membrane filter Diameter 50 mm (or 47 mm; but forregular use of this diameter, the fritssupplied have to be replaced by frits for47 mm filter, order no. 6980103)

Max. pressure only vacuumSterilization by autoclaving (121°C or 134°C), by dry

heat (180°C), with hot water or byflaming.

Order numbers for 3- and 6-branch manifold systems16824 3-Branch Manifold System 3+100 ml funnels16828 3-Branch Manifold System 3+500 ml funnels16831 6-Branch Manifold System 6+500 ml funnels16832 6-Branch Manifold System 6+100 ml funnels

32

6981001lid

6981003silicone seal


6980264clamp

6981063lid

6981064 silicone seal6981065funnel withclamp


6982119spare parts kit for tap(leaf-spring, washer and lock washer)

6980102 support frit


6980272 connector

6980271 silicone O-ring

System with suction flaskSartolab RF complete units, theall-glass filter holder type 16309and the 47 mm polycarbonateholder type 16510 are suppliedwith a filtrate receiver flask andcan be connected directly to thesource of vacuum with a vacuumhose.

All other holders must be fittedonto either a suction flask or amanifold. Additionally to thevacuum hose and source, a suc-tion flask and a suitable boredstopper is necessary.

The vacuum source can either bean electrically driven pump, awater jet pump or a hand operat-ed pump. It has to be avoidedthat filterable liquid flows intothe electric pump. This can beachieved by using a Vacusart unitwith hydrophobic PTFE filterwhen the suction flask is filled orfoamed over. When a waterpump is used, this combinationavoids that water flows back intoa suction flask. Alternatively, theuse of a Woulff’s bottle makes avisual control possible. Such abottle is also useful for a simpleadjustment of vacuum with filterholders without tap for vacuumcontrol.

Systems with manifoldsPolycarbonate holders type16511 can be fitted directly onto3- or 6-branch manifolds. Glassholders require adapters.

Also stainless steel holders can bemounted by using adapters andmulti-manifolds. The completemanifold systems described onthe previous page are far lessexpensive and more convenientto use.

Additionally, a vacuum hose anda source are required. When anelectrical pump is used, a vacuumresistant container must beinserted between manifold andpump. The insertion of a Vacusartunit in the vacuum line betweenthe container and the pump isrecommended.

Further useful accessoriesForceps are very important forthe use of the membrane filters.

A prefiltration attachment(16807) is useful when colonycounts are required from parti-cle-rich samples. It clips inbetween the filter support andthe funnel of stainless steel hold-ers to allow the simultaneous useof a particle-removing prefilterabove the bacteria-retentive fil-ter, so that resulting colonies arenot hidden by a layer of particles.

The 47 mm glass holders forsterility testing are part of theSartorius Sterility Testing System,a re-usable system which isdescribed in detail in a separatebrochure (SL-3008-e, free avail-able on request).

An anaerobic container, a ball-point pen type colony counterand a laboratory incubator com-plete the accessories offered.

1. Recommended Accessoriesfor complete filtration systems:

1a. For 25 mm glass holders16306 and 16315System with suction flask: 16672 suction flask17174 stopper16623 vacuum hose16610 Woulff’s bottle16692 or 16695 vacuum pumpManifold system:16826 3-branch manifold3+16836 adapter16623 vacuum hose17804M Vacusart16692 or16695 vacuum pumpand a vacuum-resistant container.

1b. For 50 mm glass holders16307 and16316System with suction flask:16672 suction flask17175 stopper16623 vacuum hose16610 Woulff’s bottle16692 or 16695 vacuum pumpManifold system:16826 3-branch manifold3+16837 adapter16623 vacuum hose17804M Vacusart16692 or 16695 vacuum pumpand a vacuum-resistant container.

1c. For all-glass holder 1651016623 vacuum hose16610 Woulff’s bottle16692 or 16695 vacuum pump

1d. For 47 mm polycarbonateholder 1630916673 hand-operated pump

Accessories for vacuum filter holders and manifold systems.

33

Accessories | Vacuum filtration

1e. For 47 mm polycarbonateholder 16511System with suction flask:suction flask and fitting stopperwith 20 mm hole (outer dia-meter) for the outlet spout. 16623 vacuum hose16610 Woulff’s bottle16692 or 16695 vacuum pumpManifold system:16826 3-branch manifold16623 vacuum hose17804M Vacusart16692 or 16695 vacuum pumpand a vacuum-resistantcontainer.

1f. For Sartolab RF completeunits16673 hand-operated pump

1g. For stainless steel holders16201, 16219 and 16220System with suction flask: 16672 suction flask, 2 l17173 bored stopper16623 vacuum hose17804M Vacusart16692 or 16695 vacuum pump

1h. For manifold systems16824, 16828, 16831 and1683216623 Vacuum hose17804M Vacusart16612 or16615 vacuum pump16672-1 suction flask, 5 l

including stopper andadapter

Colony counterOrder number: 17649This compact, battery operatedcolony counter is as simple to useas a ball-point pen, and has a 4-digit LCD-display. The counteris supplied with an additionalmarker refill.Order number for replacementpart: 6981540 black marker refill

Container for anaerobic incu-bation Order number: 16671Stainless steel container with11.8 cm inner diameter and10.7 cm depth, with metal insertfor convenient insertion andremoval of Petri dishes.

Transparent plastic lid holds twotaps (for the vacuum exhaust andfor cleaning with inert gas), with6 mm hose nipples (for 16623),vacuum gauge and sealing ring.For up to fourteen 60 mm, or upto six 90 mm Petri dishes.

Laboratory incubator (230 V, 50/60 Hz) Order number: 18113Compact, space saving incubatorfor the incubation of membranefilters on nutrient pads or othernutrient media, with room for6 stacks of 6 Petri dishes. Theswing-up cover and removableinsertion plate simplify loadingand unloading. The cover isopaque avoiding light penetra-tion into the chamber.

Dimensions (W+H+D): Inner 270+205+288 mm Outer 340+270+431 mm Max. load for insertion plate: 5 kgRated power: 0.2 kWTemperature range: 20°C (or 5°C above room temperature)to 50°CTemperature deviation over lessthan ±0.2°C (at 37°C and RT20°C). Spacial temperature devia-tion less than ±0.8°CVolume: ca.15 litersWeight: 5.5 kg

Rubber vacuum hose (1 meter)Order number: 16623Thick walled rubber hose forconnecting suction flasks, vacu-um pumps, etc. When ordering,please state length required inmetres.

34

2. Description of accessoriesSuction flask, 2 litre capacityOrder number: 16672Vacuum-resistant flask made ofDuran 50 glass with plastic safetyhose nipple according to theGerman Industrial Standard no.12476. Outer diameter of thehose nipple, 9 mm. Inner dia-meter of the opening, 60 mm.Stoppers are not enclosed. Ordernumbers for bored stoppers forthe outlet spouts of the variousvacuum filtration units: 17173 for 50 mm stainless steelholders17174 for 25 mm glass holders17175 for 50 mm glass holders

A 1 liter capacity flask is availablefor countries which do not havesafety restrictions on glass hosenipples. Order number: 16606

Order numbers for stoppers forthis flask:17004 for stainless steel holders,17005 for 25 mm glass holders,17005 for 47/50 mm glassholders.

Woulff’s bottle, 500 ml Order number: 16610Used between suction flask andvacuum source. Allows simplecontrol of the vacuum with glassunits without a separate tap andprevents furthermore the filtratefrom overflowing from thesuction flask.

Hand operated vacuum pumpwith gaugeOrder number: 16673Practical vacuum source, alsooutside of a laboratory. Up to80% vacuum can be obtained.The body is of PVC. Suppliedcompletely with gauge, vacuumrelease lever and a 60 cm lengthof clear plastic tubing.

Stainless steel manifoldsOrder numbers:16826 3-branch manifold16829 6-branch manifold3 or 6 polycarbonate holders ofthe type 16511 can be screweddirectly. 50 mm glass units(16307 or 16316) can be fitted byusing corresponding adapter-/stopper-combinations, ordernumber 16837 (pack of 1).

Each manifold arm has a 3-waytap for turning on and off thevacuum, and for venting theattached filter holder separately. Autoclavable at 121°C.

Replacement parts see diagram.

Water jet pump with G3/4female thread Order number: 16611Simple vacuum source. For con-nection to a water tap with G3/4male thread.

35


6980271silicone O-ring16+2 mm

6980235silicone O-ring14+2 mm (pack of 3)

6980267stainless stell tap

6980268knurled nut

6980271silicone O-ring16+2 mm

6980272hose nipple

Laboratory vacuum pump, 90%Order numbers: 16692 (220 V, 50 Hz)16695 (110 V, 60 Hz)Compact, reliable and oil-freemembrane pump with low noiselevel. Max. vacuum: 90%(100 mbar, 76 torr) Max. flow rate: 20 l/min Wattage: 80 W Weight: 4.5 kg Dimensions: 203+145+187 mm Max. ambient temperature: 40°C Replacement part: Order number6986105 (set of one neoprenemembrane, two valve springs andone neoprene head seal).

Laboratory vacuum pump, 98%Order numbers: 16612 (220 V, 50 Hz) 16615 (110 V, 60 Hz)Membrane pump with highperformance, reliable vacuumsource, oil-free. Max. vacuum: 13 mbar (10 torr) Max. flow rate: 26 l/min Wattage: 120 W Current: 1.8 Amp Weight: 9.8 kg Dimensions: 338+250+225 mmMax. ambient temperature: 40°CReplacement part: order number6986017 (set of two neoprenemembranes, four valve springsand two neoprene head seals).

Stainless steel forceps Order number: 16625Membrane filters should only behandled with suitable forceps in order to avoid contaminationwhich can result from handcontact. Sartorius stainless steelforceps can be flamed and areautoclavable. They have smooth,flat tips for a careful, firm hold ofthe membrane filter.

Water trap, Vacusart Order number: 17804 M (pack of 3)Vacusart is a ready-to-connectfiltration unit, consisting of apolypropylene housing and awater-repellent, but porous PTFEmembrane with a pore size of0.45 µm. Vacusart is perfectlysuitable for the protection ofvacuum pumps.

Positioned between suction flaskand vacuum source, it stops asudden overflow of filtrate andavoids that filtrate drops into thepump and is sprayed into thewaste air as aerosol. Connectedwith a water jet pump, it pre-vents water from flowing backinto the suction flask.

On longer use, condensed watercan restrict the porosity in thepores of the PTFE membranefilter, and reduces the filtrationspeed. The water can be remediedby blowing the Vacusart dry fromthe reverse direction, or the unitcan be dried at 105°C in a dryingcabinet overnight.

Stainless steel prefilter holder Order number: 16807Allows the removal of coarse,solid particles from samples forbacteriological testing before and during the actual bacteriaretentive filtration. The device isclipped between funnel and baseof the stainless steel vacuumfilter holders.

A bacteriological prefilter(11301-050) placed on the holderretains coarse particles from thesample, which flows through themembrane filter down into thetest filter of the filter supportbelow. After completed filtration,the test filter is incubated, andthe colonies can grow on thefilter surface without disturbancefrom, or being hidden by, anexcess of particles.

Can be autoclaved and flamed.Replacement part: 6981139support plateOrder number for cellulosenitrate membranes with 50 mmdiameter and 8 µm pore size forthe prefilter holder: 11301-050 ACN (pack of 100,sterile and individually packed)

Peristaltic pump16697---00

SpecificationMaximum rotor speeds:50 rpm and 400 rpmOperating voltages and frequencies:110–240 V 50/60 HzSpeed control ratio:20:1Power rating:100 VAOperating temperature:5°C to 40°CStorage temperature range:–40°C to 70°CWeight:5.35 kg, 12 lbNoise:<70 dBA at 1 mStandards:IEC 335-1, EN 60529 (IP31)Machinery Directive: 98/37/EC EN 60204-1Low Voltage Directive: 73/23/EEC EN 61010-1EMC Directive: 89/336/EEC EN 50081-1/EN 50082-1

36

1. Sterisart NFSterisart is a completely closedsystem for the sterility testing of pharmaceutical products. It isbased on the membrane filtermethod, however it eliminatesthe procedure of manipulatingthe filters. By this the main riskof a secondary contaminationand false positive results is alsoeliminated.

A peristaltic pump transfers thesample into the filtration units,and after rinsing the filtrationunits are filled with media andused for the incubation of thefilters without any contact to theenvironment.

2. Re-usable sterility test systemRe-usable sterility test system forthe sterility testing of injectionand infusion solutions. The filterholders are easy to clean, dish-watersave and autoclavable. Thesystem can be composed accord-ing to the needs of the user, and the membrane filter can bechosen according to require-ments.

Please ask for more infor-mations! Special brochureavailable on request.

Technical SpecificationsPumping performance: 10–700 ml/min (dependent on the tubing)Noise level at 1 m distance: < 45 dB (A)Voltage: 90–230 VAC (a. c. voltage)Tolerance range acc. to VDE: –15/+10%Frequency: 47–63 HzPower consumption: 100 WFuse: T 1,6 AAmbient temperature: +10°C – +40°CProtection class: IP 20Cooling: Self-convection and fanDimensions: 420+220+120 (W+H+D)Weight: approx. 11 kg

Ordering InformationOrder No. Description16413 Sterisart Universal-pump

Additional accessories are available on request, such as a pump cover for Millipore sterility test units, order number 1ZG 0004.

Recommended disposable sterility test units for use with pump.Order No. Description 16466 ACD Sterisart®NF alpha, dual-needle metal

spike for closed containers (box of 10,individually sterilized with ETO; single-packed)

16467 ACD Sterisart®NF alpha, 6-cm metal needle foropen containers (box of 10, individuallysterilized with ETO; single-packed)

16466 GBD Sterisart®NF gamma, dual-needle metalspike for closed containers (box of 10,individually sterilized, double-packed, optimal for use in isolators)

16467 GBD Sterisart®NF gamma, 6-cm metal needlefor open containers (box of 10, individually sterilized, double-packed,optimal for use in isolators)

Further units on request: 16464ACD, 16464GBD

Specifications of the Filter HoldersMaterial: Glass cylinder; polypropylene base and

sealing plug; anodized aluminum closingcap

Sealing: Silicone gasket, 36/47 mm (6980573)Silicone O-ring, 40.5+3.5 mm (6980574)

Filter diameter: 47 mmFiltration area: 12.5 cm2

Capacity: 16522: 85 ml (60 ml up to the mark foranaerobic incubation at a filling level of 70 mm)16523: 130 ml (56 ml up to the mark for aerobic incubation at a level of 60 mm,110 ml up to the mark at the 115-mmlevel)

Operating pressure: vacuum onlySterilization: Autoclaving at 121°C

Order No. Description 16522 filter holder with 85 ml capacity16523 filter holder with 130 ml capacity16826 stainless steel manifold16696 peristaltic pump

Sterility test systems.

37


Filtration units and devices for the pressure filtration of liquids.

Sartolab® P20 and Sartolab® P20plus for reliable sterile filtration of tissue culture solutions.

Ready-to-use units which,attached to a membrane pump ortube pump, quickly and reliablysterilize 100 ml to 5 litre ofmedia and aqueous solutions.

The combination of a large filtra-tion area (20 cm2) and an auto-matic de-aeration ensures highflow rates and optimal totalthroughputs. Any trapped airvents through a water-repellentPTFE membrane, ensuring thatthe whole filter surface is utilizedduring filtration.

Sartolab P20 units have an extra-ordinarily favourable price-per-formance ratio. Often, the totalfilterable volume can even bedoubled due to an integratedbinder-free glass fibre prefilter.

Top part: results with conta-minated medium (DMEM + 10%FCS), at 1 bar differentialpressure.

Specifications for Sartolab P20 unitsConnectors inlet, luer lock inner cone or 6–12 mm

stepped hose nipple. Outlet, 6–12 mmstepped hose nipple

Biosafety pass the USP Plastics-Class VI-TestBubble point with water, minimum value 3.2 bar

(320 kPa)Flow rate for water 250 ml/min at ∆p = 1 bar

(100 kPa)Filtration area 20 cm2

Filling volume 6 mlHousing burst pressure > 5 bar (500 kPa)Materials cellulose acetate membrane filter (0.2 µm).

PTFE-Luftfilter. Polycarbonate housing Max. recommended inlet pressure 3 bar (300 kPa)Protein adsorption less than 10 µg γ-Globulin/cm2

Hold-up volume 0.3 ml after (1.3 ml before) bubble pointToxicity non-toxic as confirmed with L929 fibrolast

cells of mice and with MRC-5 lung cells ofhuman embryonic origin

Accessories: integrity holder 18099

Specifications for Sartolab P20plus unitsAs for P20, except: Filling volume 5.5 mlMaterials supplemented with a binder-free glass

fibre prefilterProtein adsorption varies due to the prefilter Hold-up volume 0.9 ml after (1.8 ml before) bubble point

Order numbers for Sartolab P20 units18052 D with hose nipple inlet connection,

pack of 1018053 D with luer lock inlet connection, pack of 10

Order numbers for Sartolab P20plus units18056 D with hose nipple inlet connection,

pack of 1018058 D with luer lock inlet connection, pack of 10

Recommended accessories are described under 1a on page 53

40

100

150

100

50

0 10 50 150 200 time (secs)

V (ml)Sartolab-P20 plus

Sartolab-P20

Competitive mixed ester

Competitive PVDF

Newly developed, ready-to-usepressure filtration units offeringmaximum convenience for thesterile filtration of 0.1 to 50 litresof buffers, infusion solutions,tissue culture solutions, sera andother solutions containing pro-teins. No more problems with airbubbles in the liquid. A hydro-phobic PTFE membrane validatedfor sterile air filtration allows aneffective air bubble collection atthe highest point upstream.

At the beginning of the filtrationthe threaded closure can beopened, so that air bubbles canvent away and full use of thecomplete filter area is guaran-teed.

During this venting, the PTFEmembrane prevents liquid fromemerging, so protecting the fil-trate from non-sterile drops, andthe environment and user frompossible contamination. For thesubsequent integrity test, theoutlet spout has to be closedwith the closure.

Sartobran 150 and Sartobran 300filter capsules contain the sameheterogeneous surfactant-freecellulose acetate double mem-brane with low adsorption asused in larger Sartobran capsulesand Sartobran cartridges. Theydemonstrate the same superiorhigh flow rates and largethroughputs per filtration area.Furthermore, a scale-up to thelarger is only a matter of simplemultiplications, allowing you toreduce validation costs.

Specifications for Sartobran 150 and Sartobran 300 capsulesConnectors Sartobran 300: hose nipple inlet and out-

let (6–12 mm) Sartobran 150: stepped hose nipple inletand outlet (6–12 mm) or 1/2” triclamp

Biosafety pass the USP Plastics-Class VI-TestBubble point with water, minimum value 3.2 bar

(320 kPa)Chemical compatibility for aqueous solutions of ph 4–8 as well as

most alcohols and hydrocarbonsFiltration area 150 cm2 and 300 cm2

Materials Cellulose acetate membrane filter(0.45 µm or 0.2 µm pore size). PTFE airfilter (0.2 µm). Polypropylene housing andfilter support. Polycarbonate filling bell

Max. differential pressure 4 bar (400 kPa) at 20°C, 2 bar (200 kPa) at 80°C

Sterilization Supplied steam sterilized. Can be re-sterilized by autoclaving at 121°C

Cytotoxicity Non-toxic as confirmed with L-929 fibro-last cells of mice and with MRC-5 lungcells of human, embryonic origin.

Sartobran 300 Sartobran 150 (type SS)

Order numbers for Sartobran 150 capsulesSterile, individually packed:

5231307H4 OO B Hose nipple inlet and outlet, pack of 55231307H4 SS B 1/2” triclamp inlet and outlet, pack of 55231307H4 SO B 1/2” triclamp inlet, hose nipple outlet,

pack of 5

Order numbers for Sartobran 300 capsulesSterile, individually packed:

5231307H5 OO V Hose nipple inlet and outlet, pack of 25231307H5 OO B Hose nipple inlet and outlet, pack of 5

Recommended accessories are described under 1b on page 53

Sartobran® 150 and Sartobran® 300 Capsules. Optimum convenience for up to 50 litres. Cost-saving scale-up to larger volumes.

41

Pressure filtration units

Sartobran-300 Competitive product, 0.22 µm PVDFCompetitive product, 0.2 µm polysulfone

ml

0

200

400

600

800

1000

Comparison of filtered volumes(5 + 107 Ps. diminuta/ml)in 2 min. at 1 bar

Sartobran P® Filter elements for the filtration of protein containing solutions.

Sartobran P capsules aredesigned for maximum conven-ience and performance. They arecomplete filter units, ready toconnect and to use needing nopreceding cleaning. Althoughintended for single use, they canbe autoclaved several times andare therefore reusable if theapplication allows. The mem-branes are reinforced to increasetheir mechanical strength, forguaranteeing greatest reliabilityduring filtration and sterilization.Just as in the smaller Sartobran-300 capsules, the pleating of themembranes allows large filterareas to be sealed in small, handyunits.

The polypropylene housingcontains two membrane filters.The first, coarser membrane actsas prefilter, relieving the nextfiner membrane,which guaran-tees a reliable retain according topore size. This fractionated reten-tion of particles and microorgan-isms has a very favourable effecton the total throughput, asshown below. A solution of rela-tively high colloid content wasfiltered.

Specifications for Sartobran P filter unitsBiosafety All materials pass the USP Plastics-Class

VI-Tests.Chemical with aqueous solutions of pH 4–8 and Compatibility with most alcohols and hydrocarbons (see

page 111) Filtration area 0.05 m2, 0.1 m2, 0.2 m2 or 0.45 m2

Integrity test data All Sartobran P Capsules are integrity test-ed. Details on minimal bubble points andmaximal diffusional values are given in the“directions for use” supplied with them.

Materials Double layer cellulose acetate membrane,fleece-reinforced. Polypropylene housingand support

Max. differential pressure 4 bar at 20°C, 2 bar at 80°C Sterilization By autoclaving at 121°C, 30 min. Cytotoxicity All materials are non-toxic, as determined

with L-929-cells and with MRC-5-cells.

Order numbers for Sartobran P filter units1. With 0.2 µm final filter (and 0.45 µm prefilter): a) Type OO with hose nipple inlet and outlet: 5231307H7-OO-B 0.05 m2 filter area (pack of 5)5231307H8-OO-B 0.1 m2 filter area (pack of 5)5231307H9-OO-A 0.2 m2 filter area (pack of 4)

b) Type SS with Sanitary flange inlet and outlet: 5231307H7-SS-B 0.05 m2 filter area (pack of 5)5231307H8-SS-B 0.1 m2 filter area (pack of 5)5231307H9-SS-A 0.2 m2 filter area (pack of 4)

c) Type SO with Sanitary flange inlet and hose nipple outlet: 5231307H7-SO-B 0.05 m2 filter area (pack of 5)5231307H8-SO-B 0.1 m2 filter area (pack of 5)5231307H9-SO-A 0.2 m2 filter area (pack of 4)

d) Type RO G3/8 male thread inlet/hose nipple outlet: 5231307H8-RO-B 0.1 m2 filter area (pack of 5)5231307H9-RO-A 0.2 m2 filter area (pack of 4)

2. With 0.45 µm final filter (and 0.65 µm prefilter): a) Type OO with hose nipple inlet and outlet: 5231306D7-OO-B 0.05 m2 filter area (pack of 5)5231306D8-OO-B 0.1 m2 filter area (pack of 5)5231306D9-OO-A 0.2 m2 filter area (pack of 4)

b) Type SS Sanitary flange inlet and outlet: 5231306D7-SS-B 0.05 m2 filter area (pack of 5)5231306D8-SS-B 0.1 m2 filter area (pack of 5)5231306D9-SS-A 0.2 m2 filter area (pack of 4)

c) Type SO with Sanitary flange and hose nipple outlet: 5231306D7-SO-B 0,05 m2 filter area (pack of 5)5231306D8-SO-B 0,1 m2 filter area (pack of 5)5231306D9-SO-A 0,2 m2 filter area (pack of 4)

* Also available as Mini cartridges with the same pore sizes and areas.Order numbers for packs of 5:

areapore size: 0.05 m2 0.1 m2 0.2 m2

0.2 µm 5231507H7B 5231507H8B 5231507H9B

42

25

50

75

100

125

Filter combination 0.2 µm only 0.2 µm + 0.2 µm 0.2 µm + 0.45 µm

Total volume filtered

Type 00(hose nippleinlet and outlet)

Type SS(Sanitaryflange inlet and outlet)

Type RO(G3/8 malethread inlet andhose nippleoutlet)

Type SO(Sanitaryflange inlet andhose nippleoutlet)

Each of these ready-to-connectunits contains a multi-step com-bination of filters for effectiveand economical particle removal.These filters are either used aloneor as a prefilter in combinationwith a Sartobran P or Sartofluorcapsule. There is a choice of fourdifferent types, differing only inthe filters they contain. All otherparts are the same and made ofpolypropylene.

1. Sartopure PP2 CapsulesDepth-type filters containingprogressively finer polypropylenefleeces for the retention of parti-cles by fractionated depth filtra-tion. Six retention efficiencies of20, 8, 5, 3,1.2 and 0.65 µm. Majorapplications: particle removingfiltration out of deionized water,pharmaceutical solutions,reagents, chemicals, acids, sol-vents, air and other gases. 2. Sartopure GF2 CapsulesThese contain progressively finerglass fibre and polypropylenefleeces for the retention of parti-cles and some colloids by thecombination of fractionateddepth filtration and adsorption.Three retention efficiencies of 3,1.2 and 0.65 µm.Major applications: prefiltrationof biological liquids of relativelyhigh colloid content (such assera), particle removal out ofdeionized water and weaklyaggressive chemicals.3. Sartoclean CA CapsulesAvailable with 3.0 on 0.8 µm and0.8 on 0.65 µm cellulose acetatedouble membrane for the reten-tion of particles and largermicroorganisms by fractionatedmembrane filtration, and assingle layer capsules with a poresize of 0.2 and 0.45 µm.Major application: prefiltration in combination with a subse-quent Sartobran P capsule forhigher filterable volumes in thesterile filtration of serum withminimal adsorption.4. Sartoclean GF CapsulesTwo types, like Sartoclean CAcapsules, but additionally with aglass fibre prefilter for the reten-tion of particles, larger micro-organisms and colloids, by a com-bination of depth filtration andfractionated membrane filtration.Major applications: prefiltrationof biological liquids with rela-tively high colloid content.Clarification of turbid solutions.

Specifications for Sartopure PP2 and Sartoclean Capsules

Biosafety All materials pass the USP Plastics-Class VI-Test.

Filter area 0.03, 0.05, 0.1, 0.2 or 0.3 m2, as listed under order numbers.

Order numbers for Sartopure PP2 and Sartoclean Capsules*1. Sartopure PP2 depth filter capsules:1a. with 10 mm hose nipple inlet and outlet: 5591305P7-OO-B 0.65 µm, 0.05 m2 (pack of 5) 5591305P8-OO-B 0.65 µm, 0.1 m2 (pack of 5) 5591305P9-OO-A 0.65 µm, 0.2 m2 (pack of 4) 5591303P7-OO-B 1.2 µm, 0.05 m2 (pack of 5) 5591303P8-OO-B 1.2 µm, 0.1 m2 (pack of 5) 5591303P9-OO-A 1.2 µm, 0.2 m2 (pack of 4) 5591302P9-OO-A 3 µm, 0.2 m2 (pack of 4) 5591342P9-OO-A 5 µm, 0.2 m2 (pack of 4) 1b. with Sanitary flange inlet and outlet:5591305P8-SS-B 0.65 µm, 0.1 m2 (pack of 5) 5591303P9-SS-A 1.2 µm, 0.2 m2 (pack of 4) 1c. with Sanitary flange inlet and hose nipple outlet: 5591303P8-SO-B 1.2 µm, 0.1 m2 (pack of 5) 1d. with 1/4” male NPT inlet and outlet:5591301P7-NN-B 8 µm, 0.5 m2 (pack of 5)1e. with G3/8 male thread inlet and outlet: 5591302P8-RR-B 3 µm, 0.1 m2 (pack of 5)

2. Sartopure GF2 depth filter capsules with 10 mmhose nipple inlet and outlet:5571305P9-OO-A 0.65 µm, 0.2 m2 (pack of 4)5571303P9-OO-A 1.2 µm, 0.2 m2 (pack of 4)

3. Sartoclean CA membrane filter capsules3a. with 10 mm hose nipple inlet and outlet: 5621307A9-OO-A 0.2 µm, 0.3 m2 (pack of 4)5621306A9-OO-A 0.45 µm, 0.3 m2 (pack of 4)5621305G9-OO-A 0.8 | 0.65 µm, 0.2 m2 (pack of 4)5621304E9-OO-A 3.0 | 0.8 µm, 0.2 m2 (pack of 4)3b. with Sanitary flange inlet and outlet:5621307A9-SS-A 0.2 µm, 0.3 m2 (pack of 4)5621306A9-SS-A 0.45 µm, 0.3 m2 (pack of 4)5621305G9-SS-A 0.8 | 0.65 µm, 0.2 m2 (pack of 4)5621304E9-SS-A 3.0 | 0.8 µm, 0.2 m2 (pack of 4)

4. Sartoclean GF Membranfilter capsules4a. with 10 mm hose nipple inlet and outlet: 5601305G9-OO-A 0.8 | 0.65 µm, 0.2 m2 (pack of 4)5601304E9-OO-A 3.0 | 0.8 µm, 0.2 m2 (pack of 4)4b. with 10 mm Sanitary flange inlet and outlet: 5601305G9-SS-A 0.8 | 0.65 µm, 0.2 m2 (pack of 4)5601304E9-SS-A 3.0 | 0.8 µm, 0.2 m2 (pack of 4)

* Further information on Mini Cartridges and Capsulesin the brochure S--0024-d | S--0024-e.

Capsules for the particle removing filtration or prefiltration of 100 litres and more.

43

Capsule filters

See 1a, 2, 3a, 4a.

See 1b, 3b, 4b.

See 1e.

See 1c.

Easy to handle, ready to connect complete units for the wash water filtration in hospitals.

It is a well-known fact that manyinfections occuring in hospitalsare caused by tap water used forthe patients’ personal hygiene(e.g. washing, showering) or toclean instruments (e.g. rinsing ofendoscopes). For hospital areaswhere high standards of hygieneare required, sterilizing filtrationof drinking and service water atthe point of use is recommended.

The successful use of SartoriusCapsules in actual day-to-day useis well documented by reports of hygiene specialists. The cap-sules are re-usable completeunits without expensive stainlesssteel housings. The compact form of the units with smoothexternal surfaces meet thehygiene requirements. They arelight in weight and thereforevery user convenient, as thesnap-on connectors enable aneasy and rapid installation ontaps or directly in front of showerheads. The double-layered mem-branes are validated for steriliz-ing filtration, and have bacteriaretention ratings that exceedstandard requirements to ensurea high margin of safety.

Biosafety All components pass the USP Plastics–Class VI-Test.

Bubble point With water, min. value 3.2 bar (320 kPa)

Flow rate For water at ∆p = 3 bar (300 kPa), ca. 12 l/min

Final pressure Max. ∆p = 4 bar (400 kPa) at 20°C, 2 bar (200 kPa) at 80°C

Filtration area 0.1 m2 (size 8) | 0.05 m2 (size 7)

Materials Cellulose acetate membrane filter (double-layered,0.45 µm on 0.2 µm pore size), polypropylenesupport and housing

Sterilization By autoclaving (121°C, 1 bar, 30 min or 134°C, 2 bar, 15 min)

Order numbers for wash water capsules5 capsules in a pack, sterile, individually packed:

5231307H8-PQ-B inlet: 6mm quick connect coupling; outlet: integrated PP-showerhead

5231307H8-PO-B inlet: 6mm quick connect coupling; outlet: hose barb

5231307H8-VQ-B inlet: 6mm quick connect coupling; outlet: integrated PP-showerhead

5231307H8-VO-B inlet: 6mm quick connect coupling; outlet: hose barb

5231307H8-VZ-B inlet: 6mm quick connect coupling; outlet: G1/2 male thread for installation of a separate autoclavable showerhead

5231307H7-PQ-B inlet: 6mm quick connect coupling; outlet: integrated PP-showerhead

5231307H7-PO-B inlet: 6mm quick connect coupling; outlet: hose barb

5231307H7-VQ-B inlet: 6mm quick connect coupling; outlet: integrated PP-showerhead

5231307H7-VO-B inlet: 6mm quick connect coupling; outlet: hose barb

5231307H7-VZ-B inlet: 6mm quick connect coupling; outlet: G1/2 male thread for installation of a separate autoclavable showerhead

AccessoriesCouplings 17712 – 8 mm quick-connect coupling

without water stop17713 – 8 mm quick-connect coupling

with water stop

Separate 17771 – autoclavable showerhead showerhead G 1 female thread

Adapters to attach 17747 – G 3/8-female threadthe quick-connect 17748 – G 1-female threadcouplings to taps 17749 – M 22 x 1-female threador fittings of 17750 – G 1-female threaddifferent 17766 – M 24 x 1 male threadthread sizes

Integrity testing 16296––05 – fully automated integrity test unit Sartocheck-Junior

17751 – Adapter Sartocheck|8 mm quick-connect coupling

44

This holder is made of stable, autoclavable polycarbonate. Thispractical holder is suitable formany simple laboratory filtra-tions. It can be connected to aperistaltic pump or a pressurecontainer. The bell-shaped baseprotects the filtrate from repeat-ed contamination while flowingin a receiver.

The holder distinguishes itself byan excellent resistance to pres-sure, achieving density by meansof simple hand-tightening. Thetransparent top part allows thevisual control of the correct seat-ing of the O-ring.

The hose nipples can be replacedby luer connectors to use it as alarge area syringe filter holder.

Specifications for the 50 mm polycarbonate filter holderChemical compatibility as for polycarbonate, polypropylene

and siliconeFlow rate for water at ∆p = 1 bar (100 kPa),

150 ml/min with 0.2 µm, 320 ml/min with0.45 µm pore size

Filtration area 12.5 cm2

Weight 83 gThreads for connectors M 12+1 female threadMaterials polycarbonate top part, base part and

hose nipple. Polypropylene filter support.Silicone O-ring (40+5 mm)

Max. operating pressure 7 bar (700 kPa)Suitable membrane filter diameter 50 mm (prefilter, 40 mm)Sterilization by autoclaving at 121°C (the material

withstands repeated cycles, providedaggressive cleaning agents are completelywashed off and that the boiler water doesnot contain anti-corrosive or anti-scalingadditives)

Order number for the 50 mm polycarbonate filter holder16508B Polycarbonate in-line pressure filter

holder, for 50 mm membrane filter (packof 5)

Recommended accessories are described under 1d on page 53


Low-cost polycarbonate holder for the filtration of litre volumes of aqueous solutions.

45

Pressure filter holders

6985004connector

6980232filter support



6980383base part

6985004connector

Chemical-resistant PTFE holders for the filtration of aggressive liquids.

47 mm holder with 200 mlcapacity

The holder does not release traceelements into the filtrate and isresistant to almost all chemicals.The Viton O-ring in the top partallows easy hand tightening, butcan be replaced by a PTFE O-ring,order no.17039). The 6 mm outletnipple is an integral part of thebase, the 10 mm inlet hose nipplecan be replaced by a G3/8 con-nector 17051.

142 mm in-line PTFE holderThis filter holder is made com-pletely of PTFE. It is clampedbetween the two metal plates ofthe holding frame. An alternativeinlet connector for the 13 mmhose nipple is the G3/8 connector(order no. 17105).

Specifications for the 47 mm, 200 ml PTFE filter holderChemical compatibility as for PTFE and VitonFlow rate for water at ∆p = 1 bar (100 kPa),

170 ml/min with 0.2 µm, 500 ml/min with0.45 µm, 1.4 l/min with 0.8 µm pore size


Thread for inlet connector M 14+1.5 male threadMaterials top part, barrel, base part, corrugated iron,

hose nipples and filter support with40+3.5 mm PTFE O-ring. Aluminiumlocking rings. 39+3.5 mm Viton O-ring(top part)

Max. operating pressure 5 bar (500 kPa)Suitable membrane filter diameter 47 mmSterilization by autoclaving (max 134°C) or by dry heat

(180°C)

Order number for the 47 mm, 200 ml PTFE filter holder16579 PTFE pressure filter holder, 47 mm, with

200 ml capacity.

Replacement parts6985000 PTFE O-ring 6985002 connector 6985001 filter support 6985011 Viton O-ring

Specifications for the 142 mm PTFE pressure filter holderChemical compatibility as for PTFEFlow rate with 0.2 µm membrane filter at

∆p = 0.5 bar (50 kPa), 1 l/min for water,1.6 l/min for ethanol

Filtration area 130 cm2

Weight 6 kgMaterials top part, base, back pressure screen,

filter support with 131+4 mm O-ring,vent valve and PTFE hose nipples.Chromium plated holding frame plates.Aluminium legs

Max. operating pressure 5 bar (500 kPa)Suitable membrane filter diameter 142 mm (prefilter, 130 mm)Sterilization by autoclaving (max 134°C) or by dry heat

(180°C)

Order number for the 142 mm PTFE pressure filter holder16540 In-line 142 mm PTFE pressure filter holder

Replacement parts6980700 back pressure 6980705 PTFE O-ring

screen 6980706 connector6980701 filter support 6980712 screw for clamp6980703 base part 6980713 aluminium legs6980704 vent valve 6985010 clamp

46

A practical holder for a lot oflaboratory filtrations. It can beattached to a tripod with thehelp of a steel rod which can bescrewed in. The hose nipple isscrewed into the side of the toppart in order to leave room for alarge filling opening. This simpli-fies the pouring-in of the sampleand the sample can be refilledwithout removing the tube con-nection to the pressure source.Leak-proof sealing is achieved by hand-tightening of theclosing ring.

For the filtration of smallvolumes (up to about 200 ml soilsamples or viscous liquids, such as oils), the holder is connecteddirectly to a pressure source. For the filtration of up to 5 litrevolumes of relatively easily filter-able liquids (e.g. buffer solutions,solutions for cell counters andtissue culture solutions), it is usedin combination with a pressuretank.

Specifications for the 47 mm, 200 ml stainless steel pressure holderChemical compatibility as for stainless steel, PTFE and silicone.

If required, the silicone O-ring in the filtersupport can be replaced by a Viton O-ring00179 or a PTFE O-ring 17038 (reduces themax. operating pressure to 4 bar!); thesilicone O-ring in the top part can bereplaced by a Viton O-ring 17145

Flow rate for water at ∆p = 1 bar (100 kPa),200 ml/min with 0.2 µm, 600 ml/min with0.45 µm, 1.3 l/min with 0.8 µm pore size


Weight 960 gThreads for the connectors M 12+1 female threadsMaterials top part, barrel, base part, corrugated iron,

closing ring, closure cap, back pressurescreen and stainless steel hose nipples1.4401 (AISI 316). PTFE-coated stainlesssteel filter support. Silicone O-rings,41+2 mm (top part) and 42+3 mm (filtersupport). PTFE-sealing (cap)

Max. operating pressure 10 bar (1,000 kPa)Suitable membrane filter diameter 47 mm (prefilter, 42 mm)Sterilization by autoclaving (max 134°C) or by dry heat

(180°C)

Order number for the 47 mm, 200 ml stainless steel pressure holder16249 Stainless steel pressure holder for 47 mm

membrane filter, with 200 ml capacity.

Recommended accessories are described under 1e on page 53


Stainless steel holder with 200 ml capacity, for the filtration of up to 5 litre volumes.

47


6980151 port closure

6986055PTFE insert

6986084top part


6982005closing ring

6986083barrel


6980737underdrain screen

6982006base part

6981288holding rod

6980801connector

6980180screen support

142 mm stainless steel holder for the filtration of up to about 50 litre volumes.

This holder is very often used inlaboratories for particle removaland for sterile filtration of several litre volumes. It has astable construction and is easy tooperate. The large filtration areaof 130 cm2 ensures high flow rate for the total filter volume.The supplied unscrewable hosenipples can be replaced by G3/8connectors, if systems withparticularly practical handling is required.

The holder is designed foreffective sterilization by auto-claving. The arrangement of theair venting valve in the top plateand the test valve in the baseplate ensures the necessaryvapour penetration. The backpressure screen has a smooth sur-face in order to avoid damages of the membrane filters, alsowhen a glass fibre prefilter is used.

The swing-out locking clampsensure a firm sealing with simplehand-tightening.

Specifications for the 142 mm holder with hose nipplesChemical compatibility as for stainless steel, PTFE, silicone and

Viton. If required, the silicone O-rings canbe replaced by EPDM O-rings (order no.6982071), Viton O-rings (6982070) or PTFEO-rings (6982072, reduce the max. operat-ing pressure to 4 bar!), and the Viton valveO-rings by EPDM O-rings (6985184) orsilicone O-rings (6985183).

Flow rate for water at ∆p = 1 bar (100 kPa), 2 l/minwith 0.2 µm, 4,5 l/min with 0.45 µm,11 l/min with 0.8 µm pore size


Weight 6 kgThreads for connectors M 12+1 female threadsMaterials top part, base, corrugated iron, locking

clamps, stainless steel legs and valvebodies 1.4401 (AISI 316). PTFE-coatedstainless steel filter support and back pres-sure screen. Silicone O-rings (130+4 mm)in the top part and filter support. Vitonvalve O-rings (3+1.5 mm). PTFE flat gasketon valves


(180°C)

Order number for the 142 mm holder with hose nipples16275 142 mm in-line stainless steel filter holder16660 laboratory tripod with special socket

(100 cm, ca. 33 mm d)

Recommended accessories are described under 1f on page 53


48

6980801 connector

6982012 top part

6980717 Viton O-ring6980656 PTFE flat gasket6980722 valve

6982001 Silicone O-ring

6980707 back pressure screen

6980708 filter support


6980702 corrugated iron

6980711 locking clamp

6980801 connector

6982043 stainless steel leg


6980712 screw

6980717 Viton O-ring6980656 PTFE flat gasket

6980722 valve


This device is perfectly suitablefor the removal of insoluble com-ponents out of samples for thedetermination of that parts ofsludge, which can be eluted withwater. Due to the 2 litre capacity,the total sample volume can befilled in with a large filling portallowing simple pouring in of theliquid. The pressure filtrationavoids the loss of volatile compo-nents. The filter are of 130 cm2

guarantees short filtration times.

The holder is also used for thesterile filtration of difficult-to-filter liquids, such as serum. Up to three membrane filterswith pore sizes getting finer indirection of the filtration areinstalled into the holder. Thefractionated retention of sus-pended matters enlarges the fil-terable volume. The swing-outlocking clamps ensure firm seal-ing with simple hand-tightening.

Specifications for the 142 mm, 2000 ml stainless steel pressure holderChemical compatibility as for stainless steel, PTFE, silicone and

Viton. If required, the silicone O-rings canbe replaced by EPDM O-rings (order no.6982071), Viton O-rings (6982070) or PTFE O-rings (6982072, reduce the max.operating pressure to 4 bar!), and theViton valve O-rings by EPDM O-rings(6985184) or silicone O-rings (6985183).

Flow rate for water at ∆p = 1 bar (100 kPa), 2 l/minwith 0.2 µm, 4.5 l/min with 0.45 µm,11 l/min with 0.8 µm pore size


Weight 12 kgThreads for connectors M 12+1 female threadsMaterials top part, base, corrugated iron, locking

clamps, legs, locking cap and valve bodymade of stainless steel 1.4401 (AISI 316).PTFE-coated stainless steel filter supportand back pressure screen. Silicone O-rings(130+4 mm) in the top part and the filtersupport. Viton valve O-rings (3+1.5 mm).PTFE sealing (valve and cap)


(180°C)

Order number for the 142 mm, 2000 ml stainless steel pressure holder16274 Stainless steel pressure filter holders for

142 mm membrane filter, with 2 litrecapacity.

Recommended accessories are described under 1e on page 53


Stainless steel holders with 2 litre capacity, for sample preparatoin and sterile filtration of serum.

49


6986055 PTFE disc 6980151 port closure

6982002 barrel

6982036 screw





6980801 connector


6980801 connector


6982036 screw


6980656 PTFE flat gasket6980717 Viton O-ring

6980722 valve


GMP-complying 142 mm stainless steel holders with sanitary flanges.

The inlet and outlet connectorsare Sanitary flanges, which areintegral parts of the top andbottom plates. They assist inmaking the holder easy to cleanand simplify the in-line installa-tion. A suitable clamp allows,with the legs removed, to adjustthe outlet at any height.

The arrangement of the airventing valve in the top part andthe sample removal/test valve in the base guarantees a safesterilization of the device withmounted filter, as well by auto-claving as by in-line vapourdeposition. The swing-out clampsensure leak-proof installation bysimple hand-tightening. The backpressure screen, very easilymounted has a smooth surface inorder to avoid damages of themembrane filter when beingautoclaved, even when no glassfibre prefilter is used.

Specifications for the 142 mm Sanitary flange holderDimensions max. height 404 mm, width 231 mm (in

height of the clamps) or 293 mm (at theend of the legs)

Chemical compatibility as for stainless steel, PTFE, silicone andViton. If required, the silicone O-rings canbe replaced by EPDM O-rings (order no.6982071), Viton O-rings (6982070) or PTFEO-rings (6982072, reduce the max. operat-ing pressure to 4 bar!), and the Viton valveO-rings by EPDM O-rings (6985184) or sili-cone O-rings (6985183).

Flow rate for water at ∆p = 1 bar (100 kPa), 2 l/minwith 0.2 µm, 4.5 l/min with 0.45 µm,11 l/min with 0.8 µm pore size


Weight 6 kgMaterials top part, base, corrugated iron, locking

clamps, stainless steel legs and valve body1.4401 (AISI 316). PTFE-coated stainlesssteel filter support and back pressurescreen. Silicone O-rings (130+4 mm) inthe top part and filter support. Viton valveO-rings (3+1.5 mm). PTFE flat gasket on valves.

Max. operating pressure at 7 bar (700 kPa)Suitable membrane filter diameter, 142 mm (prefilter, 130 mm)Sterilization by autoclaving (max 134°C) or by dry heat

(180°C)

Order number for the 142 mm Sanitary flange holder16276 142 mm stainless steel pressure filter

holder for the in-line installation, GMP-complying, with Sanitary flanges

Recommended accessories are described under 1g on page 53


50

6980740 top part




6980717 Viton O-ring

6980656 PTFE flat gasket

6980722 valve


6980656 PTFE flat gasket

6980722 valve





6980712 screw for clamp


The construction of this holder isthe same as this of the 142 mmholder described on page 50,except the legs and the numberof locking clamps. The three legsare made of stainless steel, too, in order to avoid corrosion prob-lems, as it is sometimes the casewith aluminium legs. They areshorter and screwed in verticallyto give a very stable footing tothe holder with this larger dia-meter. The swing-out mechanismof the locking clamps is verypractical, as there are 6 clamps.

The holder offers the sameadvantages for the user as the142 mm holder, but it has a fourtimes larger filtration area andcorrespondingly higher flow ratesand standing time. The filter sup-port is designed for the maxi-mum exploitation of the filterarea and the minimum flowresistance, as it is confirmed bythe steady increase of flow rateswith increasing pore sizes (seediagram).

Specifications for the 293 mm Sanitary flange holderDimensions max. height 331 mm, width 416.5 mmChemical compatibility as for stainless steel, PTFE, silicone and

Viton. If required, the silicone O-rings canbe replaced by EPDM O-rings (order no.6982077), Viton O-rings (6982078) or PTFE O-rings (6982079, reduce the max.operating pressure to 4 bar!), and theViton valve O-rings by EPDM O-rings(6985184) or silicone O-rings (6985183).

Flow rate for water at ∆p = 1 bar (100 kPa), 8 l/minwith 0.2 µm, 14 l/min with 0.45 µm,32 l/min with 0.8 µm pore size


Weight 20 kgMaterials top part, base, corrugated iron, locking

clamps, stainless steel legs and valve body1.4401 (AISI 316). PTFE-coated stainlesssteel filter support and back pressurescreen. Silicone O-rings (280+4 mm) inthe top part and filter support. Viton valveO-rings (3+1.5 mm). PTFE valve flat gasket


(180°C)

Order number for the 293 mm Sanitary flange filter holder16277 293 mm stainless steel pressure filter

holder for in-line installation, GMP-com-plying, with Sanitary flange inlet and outlet

Recommended accessories are described under 1g on page 53


GMP-complying 293 mm stainless steel holder with Sanitary flanges.

51



6980656 PTFE flat gasket6980722 valve6982022 silicone O-ring

6980714back pressure screen



6980716corrugated iron

6980718locking clamp


6980719 screw for clamp



6980656PTFE flat gasket

6980722 valve


Modular assembly system for stainless steel filter housings.

The Sartorius modular assemblysystem for filter housings com-bines highest flexibility withshort delivery periods andfavourable prices. With the helpof a special software, the mini-,standard-single- and multi-systems can be constructed byour field service locally. There is achoice of different constructionheights, different de-aerationsand tubing according to GermanIndustrial Standards DIN, the ISOand the BSOD. Furthermore, tri-clamp, Flange or tube joint con-nectors are available according tothe usual standards.

Please ask for more information.Special brochure available on request.

Stainless steel T-type for0.05 m2, 0.1 m2 and 0.2 m2

mini cartridgesStainless steel housings forliquids for the particle or sterilefiltration.

The housing features an air vent-ing valve on the inlet side. Thechange of the mini cartridge withbayonet catch.

Suitable filter cartridges onpage 65–66 and 42.

Quality standards for themodular assembly system:Material: AISI 316 LSurfaces: interior:

Ra < 0,5 µmexterior: Ra <1.6 µm

Temperature range: –10…+150°CPressure range: –1…+10 bar

(1,000 kPa)Adapter: mini: 15;

standard: 25

SpecificationsConnectors, triclamp 50.5 mm(Sanitary flange)Width, ca. 172.5 mmSurface roughness,product touching areas < 0.5 µmMaterials, stainless steel AISI 316L, silicone O-ringMax. operating pressure, 10 bar(1,000 kPa)Max. temperature, 150°C

Order number7M19LSB0021Stainless steel mini cartridgehousing for liquid filtration T-type

52

The accessories required dependon the type of the pressurefiltration unit.

Re-usable units with barrels tohold the liquid to be filtered canbe connected to a pressuresource (pressure pump or nitro-gen bottle) after insertion of themembrane filter and prefilter,and if necessary, after steriliza-tion and pouring in of the liquid.

Concerning ready-to-connectunits, devices for the conductioninstallation and mini cartridgehousings, the filterable liquid hasto be fed in on the inlet side,eigher out of an “open” containerthrough a peristaltic or impellerpump, or out of pressurized con-duction system or a pressurizedcontainer. Various systems with pressurized containers aredescribed on the next page.

1. Recommended accessories1a. For Sartolab-P20 unitsUnits with luer lock inlet connec-tor require only the pump18059,which is supplied complete withsuitable tubing.

Units with hose nipple inlet canbe connected to a peristalticpump or a pressurized containerusing commercially availabletubing.

1b. For Sartobran-300 capsulesThe hose nipple inlet can be con-nected to a peristaltic pump or apressurized container using com-mercially available tubing.

1c. For Sartobran-P capsulesConnection to a pressurized con-tainer: capsule with G3/8 malethread with inlet hose nippleusing a PTFE-tube 16999, capsulewith inlet hose nipples with com-mercially available tubing.

Connectors for capsules withinlet Sanitary flange aredescribed under 1g.

1d. For polycarbonate holder16508The inlet hose nipple can be con-nected to a peristaltic pump or apressurized container using acommercially available tubing.The hose nipple can be replacedby a connector with G3/8 malethread (order no. 17089) in orderto connect the device to a pres-surized container using a PTFEpressure hose 16999.

The hose nipple can be replacedby a luer lock connector (orderno. 16881), in order to use thedevice as syringe filter holder. A luer slip connector (order no.16880) can replace the outlethose nipple.

1e. For stainless steel holders16249 and 16274The inlet hose nipple can be con-nected to a pressure source(pump or nitrogen bottle) with acommercially available hose.Alternatively, the hose nipple canbe replaced by a connector withG3/8 male thread (order no.17089), in order to connect thedevice to the pressure sourcewith a flexible pressure hose17091, or a PTFE pressure hose16999.

For the filtration of easy to filter,large-volume liquids, the 47 mmholder can be connected to a 5 lpressurized container using aconnector with G3/8 male threadand a PTFE pressure hose.

1f. For stainless steel holder16275The inlet hose nipple can be con-nected to a peristaltic pump or apressurized container with acommercially available hose, butit is far more practical to replacethe hose nipple by a connectorwith G3/8 male thread (order no.17089), for connecting the unitto a pressurized container with aPTFE hose 16999.

However it is connected, furtheraccessories simplify the use ofthe holder, when the filtrate is tobe filled into bottles. A hand-operated valve (16656) on theoutlet side allows the control ofthe filtrate flow. A clamp (17036)replaces the three legs allowingto adjust the height of the outletto that of the bottles.

1g. For holders, mini cartridgehousings and capsules withSanitary flange inletsThe Sanitary flange at the inletand outlet require one clamp(17033) and one connector.

The outlet connector is usually a19 mm (17017) or a 25 mm(17016) hose nipple, or anadapter 17150 for the hand-operated valve (16656), withwhich the flow of the filtrate canbe regulated.

The inlet connector depends onthe system:

Connector 17019 with G3/8 malethread allows the connectionwith a PTFE pressure hose 16999to a pressurized container.

Accessories for pressure filtration units.

53

Accessories | Pressure filtration

Filtration systems with pressure tanks and three different connection possibilities.

a) With G3/8 connectorsThe pressure tank is connected to the pressure source and the filtration unit by means of stainless steelreinforced PTFE hoses. These hoses can be autoclaved and are easy to clean. Due to the density barrel in the connections, a slight tightening with a 19 mm spanner for a leak-proof sealing. No seals and Teflontapes are required.

Main advantage: easy cleaning.

b) With quick connectorsThe pressure tank is connected to the pressure source and the filtration unit by means of stainless steelreinforced PTFE pressure hoses and quick connect couplings. Hoses and couplings can be autoclaved. Thevalve in the quick connect coupling closes automatically, when the coupling is removed from the quickconnect nipple.

Main advantage: connection and removal of the coupling is quick and simple.

c) With commercially available hosesThe pressure tank is connected to the pressure source and the filtration unit by means of available pressurehoses. The hoses are to be clamped to the hose nipples.

Main advantage: hoses are usually available.

54

Pressure pump:has a suitabledensity barrelconnection.

Pressuretank:has suitabledensity barrelconnections.

Pressure hoses: have suitable density barrel connections.

Pressuretank:G3/8 connectorsare to be replacedby quickconnect nipples(17170).

Pressure tank:G3/8 connectorshave to bereplaced by hosenipples (16863).

Pressure hoses: require an additional adapter (6985128) on thenuts to the pressure tanks inlet and outlet, and to the filter units.

Pressure hoses: require tube clamps.

Pressure pump:has a suitabledensity barrelconnection.

Pressure pump:connectors must be simply reversed.

Sartobran P capsules type RO have a suitable density barrel connection.The inlet hose nipple of the holders16249, 16275 and 16508 have to be replaced by connector 17089Capsules, holders and holding withinlet Sanitary flange requireconnector 17019.

Not recommended for capsules, housings or devices with Sanitary flanges. The inlet hose nipple of the holders 16249, 16275, and 16508 have to be replaced by a connector 17090.

Sartolab P20 units, Sartobran 300 capsule, Sartobran P capsule type OO as well as the filter holders 16249, 16275 and 16508 feature hose nipples as standard.

2. Description of accessories

Membrane pump Order number: 18059

A self-priming diaphragm pumpwith electronic speed regulator,complete with connector tubingset. It pumps aqueous solutionsquietly without stressing theproduct, and is equipped with anoverpressure limiter which can beset at 1–3 bar. Pump, stainlesssteel sinker and tubing are resist-ant to 1N NaOH.

Input wattage, 15 WElectrical supply, 220 V, 50 HzDiaphragm, max. 3,000/minMaterials, polypropylene housing,PTFE membrane, EPDM seals and valvesMax. operating pressure, 3 bar,preset to 2.5 barRated output for water,650 ml/min without pressure,300 ml/min with Sartolab-P20Self-priming, up to 3 m watercolumn Power consumption,0.76 A

Replacement part: 6988094 Tubing set, sonsisting of2.5 m silicone hose (4 mm innerdiameter, 1.5 mm wall thickness,60 Shore A hadness), 5 multifunctional adapters, 1 stainlesssteel sinker

Membrane pump for pressure Order numbers:16617 (220 V, 50 Hz)16662 (110 V, 60 Hz)

Supply oil-free compressed air atup to 7 bar (700 kPa).

Connectors: G3/8 male thread inthe pressure outlet side, 9 mmhose nipple in the air inlet side.The connectors are interchange-able.

Weight, ca. 15 kgThreads for connectors, G1/4female threadDimensions, 35 + 25 + 26 cmMax. performance, 55 l/minMax. ambient temp., 40°CPower, 250 WProtection, IP 44

Replacement part:6986006 Spare parts kit,consisting of 2 membranes,4 valve springs and 2 pump headgaskets

Pressure tankOrder numbers:17530 5 litre capacity17531 10 litre capacity17532 20 litre capacity17533 40 litre capacity17534 60 litre capacity17535 80 litre capacity17536 100 litre capacity

Pressure tanks serve as reservecontainer for pressure filtration,and is also used for the transport,storage and distribution ofliquids. Two handles simplify thehandling and the transport.Special trolleys are available forthe 40, 60, 80 and 100 litrepressure tanks.

The pressure tanks are made of1.4401 (AISI 316) stainless steel,and all surfaces are electro-polished. The tanks can be auto-claved at 121°C.

The screwed on G3/8 connectorsallow the connection of PTFEpressure hoses 16823 or 16999.They can be replaced by hosenipples, Sanitary flanges orconnectors for quick connectsystems (see accessories).

55


As standard, the lid is equippedwith a pressure gauge, a safetyvalve, and a clamp for leak-proof,pressure-resistant closure. A certificate concerning con-struction and pressure testingaccording to the German decreefor pressure tanks is enclosed to every tank (the tanks arespecifically designed for pressure,and are not usable as vacuumcontainers).

Specifically for the requirementsof the pharmaceutical industry,GMP-complying pressure tanksare on request available in vari-ous sizes. Benefits of the deviceare the ease of cleaning, theequipment with triclamp connec-tors on standard and the lowsurface roughness.

SpecificationsDimensions (height + diameter)/weight:17530 235 + 234 mm/3.9 kg17531 360 + 234 mm/5.4 kg17532 600 + 234 mm/8.2 kg17533 705 + 300 mm/11.8 kg17534 643 + 400 mm/15.2 kg17535 802 + 400 mm/18.4 kg17536 962 + 400 mm/21.7 kg(opening, for all types, oval,length 98 mm, width 82 mm)

Maximal operating pressure: 7 bar for 17530, 17531, 17532.5 bar for 17533.3 bar for 17534.2 bar for 17535, 17536

Max. operating temperature:95°C

Accessories6985093 Spanner, 17–19 mm (to fasten connectors)

17636 Trolley for 1753317635 Trolley for 17534,

17535 and 17536

The silicone O-rings supplied on standard can be replaced by the following Viton or EPDM O-rings:

6986110 Silicone O-ring (lid)6986132 Silicone O-ring (tubes)6986111 EPDM O-ring (lid)6986133 EPDM O-ring (tubes)

Other connectors:16863 Hose nipple, DN 10–1917070 1”–11” Sanitary flange17170 Quick connect nipple

Replacement partsa) for all pressure tanks:6980389 Viton O-ring (lid)6980395 Inlet tube6980396 Viton O-ring (tubes)

6980420 Connector, G3/86985131 PTFE cap (2 +)

b) for 17530, 17531,175326980390 Pressure gauge, 7 bar6986112 Outlet tube (17530)6986113 Outlet tube (17531)6986114 Outlet tube (17532)6986130 Lid with valve

c) for 175336980415 Pressure gauge, 5 bar6986115 Outlet tube (17533)6986129 Lid with valve

d) for 175346986116 Outlet tube (17534)6986137 Pressure gauge, 3 bar6986138 Lid with valve

e) for 17535, 175366986117 Outlet tube (17535)6986118 Outlet tube (17536)6986119 Pressure gauge, 2 bar6986131 Lid with valve

56

PTFE pressure hoseOrder numbers:16999 1.5 m long16823 80 cm long

Stainless steel reinforced PTFEpressure hoses with G3/8 nuts oneach side. The hoses are solventresistant and easy to clean. Theycan be sterilized by autoclaving(121°C or 134°C) or by dry heat(180°C).

The nuts fit onthe G3/8 malethreads, andensure a leak-proof connection

without the need for sealingrings or Teflon tapes.

The nuts also fiton a function-piece with quickconnect coupling(order no.

6985128) for a quick and simpleconnection to holders fitted withquick connect nipples. The valvein the coupling opens, when it isfitted on a quick connect nipple,and closes when removed fromthe nipple.

Accessories for 6985128:

6980407 Trigger valve forcleaning

Replacement part for6985128:6985216 Seal set (Viton O-ring,

flat gasket)

Flexible pressure hoseOrder number: 170911 m long. G3/8 nuts on each side.It is very flexible and especiallypractical as pressure hose forpressure holders with capacitybarrel. Can be sterilized by auto-claving or by dry heat. Not foruse with liquids.

Plastic pressure hoseOrder number: 16931Flexible gas pressure hose withquick connect coupling for directconnection to pressure holderswith capacity barrel, fitted withquick connect nipple and withG3/8 nut for connection to thepressure source. Not for use withliquids.

Hand-operated valveOrder number: 16656

This valve is fitted on the outletside of a filter holder type 16275,and allows a steady regulation ofthe filtrate or a selective dosagewhen filling up litre volumes.

An adapter (order no. 17150)allows the attachment of a cap-sule and a mini cartridge housingwith Sanitary flange.

Fitted to the filter holder, thevalve can be sterilized, whenopen, with all the usual methods.For cleaning purpose, it can bequickly taken apart withoutproblems.

Materials: ball and housing,stainless steel (material no.1.4401, AISI 316). Seat and nipplefor 13 mm hose, PTFE.

Replacement parts:6981314 Stainless steel bell6986090 Valve body6986091 Connector, M12+16986092 PTFE hose nipple6988093 PTFE sealing, (pack of 2)

Clamp for Sanitary flangesOrder number: 17033

Two 1–11”Sanitary flangesare pressedagainst the sup-plied gasket andare attached withthe clamp.

Replacement silicone gaskets areavailable under the order number6982029 (pack of 2). Ethylenepolypropylene gaskets (order no.6982060) and PTFE reinforcedbuna (6982061) are also avail-able.

57


Ready to connectventing units, Pressure holders, Mini cartridge housingsand Mini cartridges.

Midisart® 2000 sterile venting units, light in weight and easy to connect.

Re-usable complete filtrationunits with naturally hydrophobicPTFE membrane for reliablesterile venting of small fer-menters and of containers forculture media.

Midisart 2000 units have beendesigned for maximum handlingease and reliability. Tapered hosenipples ensure a simple, securehold for tubing with an innerdiameter of 6–12 mm. Due to thelow weight of only 20 g, theconnected tubing is not snappedoff. The membrane is reinforcedwith polypropylene gauze forstability to pressures of up to3 bar. The 20 cm2 large filter areaallows high flow rates at lowdifferential pressures.

Each unit is printed with lotnumber and individual piecenumber on the housing for totalsecurity and traceability.

Specifications for Midisart 2000 unitsConnectors Choice of conical hose nipples for tubing

with 6–12 mm inner diameter (with slip-fit for luer syringes), or 1/8” male NPT

Biosafety All materials pass the USP Plastics Test Class VI.

Bubble point Min. value with isopropanol for 0.2 µm unit = 1.4 bar (140 kPa) (1.1 bar after autoclaving) and 0.9 bar (90 kPa) for 0.45 µm unit

Air flow rate Typical values for 0.2 µm pore size: 1.1 l/min at 0.02 bar (1.8 l/min for 0.45 µm)2.0 l/min at 0.05 bar (4.6 l/min for 0.45 µm)5.0 l/min at 0.1 bar (8.5 l/min for 0.45 µm)

Filter area 20 cm2

Filling volume Ca. 3 mlHousing diameter 62 mmMaterials PTFE membrane filter aus PTFE, reinforced with

polypropylene gauze, polypropylene housingMax. recommendedoperating pressure 3 bar (300 kPa) Max. temperature 134°CSterilization method By autoclaving at 121°C (at least 20 times) or

134°C. E and G packs are presterilized with eth-ylene oxide.

Hold-up volume Ca. 0.5 ml after (1 ml before) bubble pointWater penetration point 4.0 bar (0.2 µm) and 3.0 bar (0.45 µm)

Order numbers for Midisart 2000 unitsa) with hose nipple connector:17804 E 0.45 µm, sterile, individually packed (pack of 12)17804 G 0.45 µm, sterile, individually packed (pack of 25)17805 E 0.2 µm, sterile, individually packed (pack of 12)17805 G 0.2 µm, sterile, individually packed (pack of 25)17805 UPN 0.2 µm non sterile, bulk packed (pack of 100)

b) with 1/8” male NPT connectors:17804 NPE 0.45 µm, sterile, individually packed (pack of 12)17804 NPG 0.45 µm, sterile, individually packed (pack of 25)17805 NPE 0.2 µm, sterile, individually packed (pack of 12)17805 NPG 0.2 µm, sterile, individually packed (pack of 25)

Minisart® HY ready to connect units for the sterile venting of small containers and bottlesThese 26 mm units consist of a polyester-strengthened 0.2 µm PTFE mem-brane in a cyrolite housing with luer lock connectors (female top, malebottom).

Specifications for Minisart HYBubble point Min. value with isopropanol

1.2 barAir flow rate Ca. 1.4 l/min at ∆ = 0.1 barFilter area 5.3 cm2

Housing burst pressure Min. value 6.0 bar (600 kPa)Water penetration point Min. 4.0 bar (400 kPa)

Order numbers for Minisart HY16596 HYK sterile, individually packed (pack of 50)16596 HYQ non-sterile (pack of 500)

60

a) The 25 mm filter holder

The G1/4 connection threadswith density barrel guaranteeleak-proof sealing of the hosenipple and the holder withoutsealing rings. Other connectors,available as accessories, fit theholder onto reducing valves orpumps with G1/4 female thread(order no. 01030) or G3/8 femalethread (01029), or onto pressuretanks with G3/8 male thread(00177).

SpecificationsConnectors, hose nipples DN10Filtration area, 3 cm2

Flow rate, for air at ∆p = 1 bar:0.5 l/min with 0.2 µm,1.0 l/min with 0.45 µm pore sizeWeight, ca. 170 gMaterials, stainless steel, except silicone O-ring (21+2 mm) and alumini-um closing ringMax. opperating pressure, 5 bar (500 kPa)Suitable membrane filter, 25 mm , type 118Sterilization, by autoclaving (max. 134°C) or by dry heat (max. 180°C).


Order number16251 Stainless steel holder

for 25 mm d membranefilter

25 mm and 47 mm stainless steel filter holders for in-line filtration.

61

Air | Gas filtration

6980176Connector

6981031Top part

6981033Silicone O-ring

6981032Base part

6981034Closing ring

6980176Connector

b) The 47mm filter holder

Tolerates pressure of up to20 bar. The inlet side valve isconvenient for the intermittentrun-off of waste water. Otherconnectors, available as acces-sories, fit the holder onto redu-cing valves or pumps with G3/8female thread (order no. 17089),or onto pressure tanks with G3/8male thread (17069) or on tapswith G3/4 male thread (17068).

SpecificationsConnectors, hose nipples DN10Connection thread, M12+1Filtration area, 13 cm2

Flow rate, for air at ∆p = 0.3 bar: 0.5 l/min with 0.2 µm, 1.0 l/minwith 0.45 µm pore sizeWeight, ca. 490 gMaterials, stainless steel, exceptsilicone O-ring (42+ 3 mm), PTFEand Viton valve sealsMax. operating pressure, 20 bar (2,000 kPa)Suitable membrane filter, 47 mm,type 118Sterilization, by autoclaving (max. 134°C) or by dry heat (max. 180°C).


Order number16254 Stainless steel holder

for 47 mm d membranefilter

6982005closing ring

6982003top part

6980721back pressure screen


6980180screen support

6980737support plate

6982006base plate

6980801connector

6980722valve

6980656PTFE flat gasket

6980717Viton O-ring

A B C A = 5181307T7-00B = 5181307T8-00C = 5181307T9-00

Sartofluor® Capsules with PTFE membrane for maximum security in sterile venting.

Sartofluor Capsules are ready-to-connect, pre-tested, completefilter units. The PTFE membrane is pleated to obtain the largestpossible usable filtration area inthe small polypropylene housing.The two valves on the upstreamside of the housing ensure goodsteam passage and correspondingcertainty of sterilization of thecapsules by autoclaving.

The extreme hydrophobicity of the PTFE membrane ensuresmaximum filtration security,even when filtering moist air. Thehigh air flow rate of the mem-branes and the large filter areaenable effective sterile filtrationeven at low differential pressures.

The excellent chemical compati-bility of the PTFE and polypropy-lene materials makes Sartofluorcapsules additionally useful forthe filtration of those acids, basesand non-aqueous solvents forwhich other capsule types cannotbe used.

Specifications for Sartofluor capsulesConnectors Hose nipple 10 mm or 1”–11”

Sanitary flangeBiosafety All materials pass the USP Plastics Test

Class VI.Bubble point Min. value with 60% isopropanol:

1.5 bar (150 kPa) for 0.1 µm pore size1.0 bar (100 kPa) for 0.2 µm pore size0.6 bar (60 kPa) for 0.45 µm pore size

Chemical compatibility See page 111Air flow rate For 0.2 µm capsules see diagram

bottom leftFilter area 0.015 m2, 0.03 m2, 0.05 m2, 0.1 m2 or 0.2 m2

Material PTFE membrane filter. Housing, polypropy-lene supporting and drainage layers

Max. differential pressure 4 bar at 20°C, 2 bar at 80°CMax. operating pressure 4 bar at 20°CSterilization By autoclaving (121°C or 134°C)Water penetration pressure Ca. 4.5 bar (450 kPa) for 0.2 µm pore size

Order numbers for Sartofluor Capsulesa) Sartofluor Capsules with hose nipple inlet and outlet:

5181358T7-OO-B 0.1 µm, 0.05 m2 (pack of 5)5181358T8-OO-B 0.1 µm, 0.1 m2 (pack of 5)5181358T9-OO-A 0.1 µm, 0.2 m2 (pack of 4)5181307T4-OO-B 0.2 µm, 0.015 m2 (pack of 5)5181307T5-OO-D 0.2 µm, 0.03 m2 (pack of 10)5181307T7-OO-B 0.2 µm, 0.05 m2 (pack of 5)5181307T8-OO-B 0.2 µm, 0.1 m2 (pack of 5)5181307T9-OO-A 0.2 µm, 0.2 m2 (pack of 4)5181306T9-OO-A 0.45 µm, 0.2 m2 (pack of 4)

b) Sartofluor Capsules with Sanitary flange inlet and outlet:5181307T4-SS-B (pack of 5)5181307T7-SS-B 0.2 µm, 0.05 m2 (pack of 5)5181307T8-SS-B 0.2 µm, 0.1 m2 (pack of 5)5181307T9-SS-A 0.2 µm, 0.2 m2 (pack of 4)

Capsules with other connector combinations may be availableaccording to requirements. Details on request.

Air Flow Rates at Atmosperic PressureSartofluor®-Capsules 0.2 µm, Type 5181307T4, T5 (00-connectors)

Air Flow Rates at Atmosperic PressureSartofluor®-Capsules 0.2 µm, Type 5181307T7, T8, T9 (00-connectors)

62

A B A = 5181307T4-00B = 5181307T5-00

a) Housing for sterile venting

The cut-outs in the top part ofthe housing for free air guaran-tee a good air circulation andensure the drying-out of the sys-tem after vapour deposition(avoidance of condensate forma-tion). The base has a plug for the inner O-ring and a bayonet-lock for a firm hold of the insert-ed mini cartridges.

SpecificationsConnector DN 25 tube jointWeight ca. 700 gHeight ca. 186 mmMaterial stainless steel 1.4571 (= AISI 316)Max. operating pressure 10 bar (1,000 kPa)Max. temperature 180°C

Order number350019V15-K025A Stainless steel mini cartridge housing for

the sterile venting of housings and tanks,with DN 25 tube joint and bayonet-lockfor the inserted mini cartridge.

Housings for sterile air venting and for air | gas filtration.

63

Mini cartridge housings

Housing for pressure gas filtrationThe base serves as receiver for the condensate and has also apharma-valve. The mini cartridgeholder prevents the mini car-tridge from contacting condensewater and ensures best vapourdeposition conditions. Attach-ment of the mini cartridges likefor the liquid housing, T-type(page 52).

Suitable filter cartridges onpage 64.

SpecificationsConnectors,clamp 50.5 mm (Sanitary flange)Width, ca. 164 mmSurface roughness,product touching areas <0.5 µmMaterials, stainless steel AISI 316L, silicone O-ringMax. operating pressure, 10 bar(1,000 kPa)Max. temperature, 150°C

Order number7M19LSB0028Stainless steel mini cartridgehousings for air | pressure gas filtration

A B C D A = 5181407T7B = 5181507T7C = 5181507T8D = 5181507T9

Sartofluor® Mini cartridges for highest safety for sterile venting and compressed air | gas filtration.

Sartofluor Mini cartridges aredesigned particularly for cases ofsterile venting when the cGMPdirectives have to be adhered to.

The sealing system, an inner O-ring plus bayonet twist lock,guarantees safe attachment in mini cartridge housings and a firm hold for back pressurepushes.

The inserted, specially developedPTFE membranes are extremelywater-repellent, which is shownimpressively by the very highwater penetration pressure. Thediagram shows values for various0.2 µm filter materials. Due tothe optimal hydrophobicity,steem sterilized Sartofluor minicartridges re-reach their maximalflow rates in shortest time.

Specifications for Sartofluor Mini CartridgesConnector inner silicone O-ring (replacement part no.

6985150) and bayonet lockBiosafety pass USP Plastic Class VI TestBubble point minimum value, wetted with 60%

isopropanol, 1.5 bar (150 kPa) for 0.1 µm,1.0 bar (100 kPa) for 0.2 µm, 0.6 bar(60 kPa) for 0.45 µm pore size

Chemical compatibility as for polypropylene, PTFE and silicone(silicone O-ring can be replaced by anEPDM O-ring, order no. 6985149, or aViton O-ring, order no. 6985151)

Flow rate for air for 0.2 µmMini cartridges see diagrammeFiltration area 0.05 m2, 0.1 m2 or 0.2 m2

Materials PTFE membrane filter. Polypropylenehousing protective fleece and drainagefleece. Silicone O-ring

Max. differential pressure 5 bar (500 kPa) at 20°C, 2 bar (200 kPa) at 80°C

Sterilization fitted in a mini cartridge housing, autoclaving or in-line steaming (121°C or 134°C). In-line steaming, max. ∆p = 0.5 bar

Water penetration pressure ca. 4.5 bar (450 kPa) for 0.2 µm pore size

Order numbers for Sartofluor Mini CartridgesWith 0.1 µm filter:5181558T7 B 0.05 m2 filter area, pack of 55181558T8 B 0.1 m2 filter area, pack of 55181558T9 B 0.2 m2 filter area, pack of 5With 0.2 µm filter:5181507T7 B 0.05 m2 filter area, pack of 55181507T8 B 0.1 m2 filter area, pack of 55181507T9 B 0.2 m2 filter area, pack of 5Sartofluor Junior:5181407T7 B 0.05 m2 filter area, pack of 5With 0.45 µm filter:5181506T9 B 0.2 m2 filter area, pack of 5Sartofluor Capsulen see page 62

Air Flow Rates at Atmosperic PressureSartofluor®-Mini 0.2 µm, Type 5181507T7, T8, T9, 5181407T7

64

6

Water penetration pressure (bar)

5

4

3

2

1

0

Sartofluor PVDF PTFE PTFE PVDF

Alternative products

Each of these mini cartridgescontains a series of filters withincreasing fineness for effectiveand economic particle removal,either as independent filter or asprefilter in combination with aSartobran P or Sartofluor minicartridge. The four differenttypes differ only in the filtercombinations. All other parts arethe same, made of polypropylene(support framing) or silicone(sealing ring).

1. Sartopure PP2 mini cartridgesThey contain polypropylenefleeces of increasing fineness byfractionated depth filtration.Retention efficiency: 20 µm,8 µm, 5 µm, 3 µm, 1.2 µm and0.65 µm. Main applications: particle removing filtration ofdeionised water, pharmaceuticalsolutions, chemicals and solventsand other gases.

2. Sartopure GF2 minicartridgesThey contain glass fibre andpolypropylene fleeces of increas-ing fineness for the removal ofparticles and some colloidsthrough a combination of frac-tionated depth filtration andadsorption. Retention efficiency: 3 µm,1.2 µm and 0.65 µm. Main appli-cations: prefiltration of biologicliquids with relatively high col-loid content (e.g. serum) as wellas particle removing filtration of deionised water and weaklyaggressive chemicals.

3. Sartoclean CA minicartridgesAvailable with 3.0 µm/0.8 µm and 0.8 µm/0.65 µm celluloseacetate double membranes, forthe retention of particles andlarger microorganisms by meansof a fractionated membrane fil-tration, as well as simple mem-brane mini cartridge with 0.2 and0.45 µm pore size. Main applica-tion: prefiltration in combinationwith a subsequent Sartobran Pmini cartridge (e.g. for lager fil-terable volumes in the sterile fil-tration of serum) at minimaladsorption.

4. Sartoclean GF minicartridgesSame as Sartoclean CA minicartridges, but complemented by a glass fibre prefilter for theretention of particles, largermicroorganisms and colloidsthrough the the combination of Sartopure PP2

Mini Cartridges 0.2 m2, 0.65 µm, 1.2 µm, 3 µm

Sartopure GF2Mini Cartridges 0.2 m2, 0.65 µm, 1.2 µm, 3 µm

Water Flow Rates for 0.2 m2 Sartoclean® CA andSartoclean® GF 0.8|0.65 µm

Mini filter cartridges for the particle-removing filtration or prefiltration of 100 litres and more.

65

Arbeitstechniken

Specifications for Sartopure and Sartoclean Mini cartridgesConnectors Inner Silicone O-ring and bayonet lock (twist

lock) for safe hold on the base (also refer todescriptions on p. 63 and p. 64)

Flow rate Typical values for 0.2 m2 mini cartridges forwater at 0.5 bar (50 kPa) pressure:

Sartopure PP2 39 l/min. (1.2 µm), 24 l/min. (0.65 µm)Sartopure GF2 58 l/min. (1.2 µm), 25 l/min. (0.65 µm)Sartoclean CA 41 l/min. (0.8 µm), 32 l/min. (0.65 µm)Sartoclean GF 25 l/min. (0.8 µm), 17 l/min. (0.65 µm)Filter area 0.05 m2, 0.1 m2, 0.2 m2 or 0.3 m2, as listed under

order numbersSee also diagram on the right with following classification: Filter materials Sartopure PP 2, Polypropylene filter

Sartopure GF 2, Glass fibre filterSartoclean CA, Cellulose acetate membranesSartoclean GF, Glass fibre prefilter, celluloseacetate membranes

Order numbers for Sartopure and Sartoclean Mini Cartridges*1. Sartopure PP2 Depth Filter Mini Cartridges5591505P7-B 0.65 µm, 0.05 m2 (pack of 5)5591505P8-B 0.65 µm, 0.1 m2 (pack of 5)5591505P9-B 0.65 µm, 0.2 m2 (pack of 5)5591503P9-B 1.2 µm, 0.2 m2 (pack of 5)5591502P9-B 3 µm, 0.2 m2 (pack of 5)5591542P9-B 5 µm, 0.2 m2 (pack of 5)5591501P9-B 8 µm, 0.2 m2 (pack of 5)5591520P9-B 20 µm, 0.2 m2 (pack of 5)

2. Sartopure GF2 Depth Filter Mini Cartridges5571505 P7-B 0.05 m (pack of 5) 0.65 µm5571503P7-B 0.05 m (pack of 5) 1.2 µm5571502P7-B 0.05 m (pack of 5) 3 µm5571505P8-B 0.1 m (pack of 5) 0.65 µm5571503P8-B 0.1 m (pack of 5) 1.2 µm5571502P8-B 0.1 µm (pack of 5) 3 µm5571505P9-B 0.8 m (pack of 5) 0.65 µm5571503P9-B 0.2 m (pack of 5) 1.2 µm5571502P9-B 0. 2 m (pack of 5) 3 µm

3. Sartoclean CA Membrane Filter Mini Cartridges5621507A9-B 0.2 µm, 0.2 m2 (pack of 5)5621506A9-B 0.45 µm, 0.2 m2 (pack of 5)5621505G9-B 0.8 | 0.65 µm, 0.2 m2 (pack of 5)5621504E9-B 3.0 | 0.8 µm, 0.2 m2 (pack of 5)

4. Sartoclean GF Membrane Filter Mini Cartridges5601505G9-B 0.8 | 0.65 µm, 0.2 m2 (pack of 5)5601504E9-B 3.0 | 0.8 µm, 0.2 m2 (pack of 5)

* For more information about mini cartridges and capsules, see brochure S-0024-d02025

depth and fractionated mem-brane filtration.Main applications: prefiltrationof biological liquids with arelatively high colloid content as well as clarification of turbidsolutions.

Sartopore 2 Filter elements for best flow rates and standing timesover the whole pH-range.

The new Sartopore 2 filterelements ideally supplement theSartobran P filters described onpage 42. Whereas Sartobran Pfilters are mainly used for pre-filtration and sterile filtration ofprotein-containing solutions inthe pH-range of 4–8, the broadchemical compatibility of thepolyethersulfone membranesfrom pH1 to pH 14 of theSartopore 2 filter elements allowsalso the filtratin of aggressiveliquids of high or low pH.

Sartopore 2 filter elements areavailable with 3 different poresizes. For the prefiltration ofdifficult to filter solutions,Sartopore 2 filter elements with0.45 µm final membranes areused, whereas filter elementswith 0.2 µm final membrane isused for the sterile filtration ofmedia. Sartopore 2 filter ele-ments with 0.1 µm final mem-brane are perfectly suitable forcombined sterile filtration andretention of mycoplasma in seraand serum-containing culturemedia.

The asymmetric structure of themembrane and the double-layerconstruction with build-in pre-filter allow exceptionally highstanding times and flow rates.The filter elements are thereforeused especially for the filtrationof difficult to filter, highlyviscous solutions or when shortfiltration times are required.

The graph below shows thecomparison of the total filtrationvolume of Sartopore 2 polyether-sulfone membranes, PVDF, nylon-66 membranes as well as two different PES membranes, also in the 10”-cartridge format, eachin kg filtrate at 90% blocking.

Specifications for Sartopore 2 CapsulesBiosafety All materials pass the USP Plastics Test

Class VlChemical compatibility to aqueous solutions in the pH-range 1–14Connectors see order numbersCytotoxicity All materials are detectably non-toxic

concerning L929-cells and MRC-5-cells.Filter area 0.015 m2, 0.03 m2, 0.05 m2, 0.1 m2, 0.2 m2

or 0.45 m2

Integrity test data All Sartopore 2 Capsules are integritytestable. You find detailed informationabout minimal bubble points and maximalair diffusion values in the instructions for use, enclosed to every pack.

Materials Asymmetric, double-layered polyether-sulfone membrane filter, polypropylenehousing parts and support framing |drainage devices

Max. differential pressure ∆p = 4 bar at 20°C, 2 bar at 80°C

Order numbers for Sartopore 2 Capsules*1. Sartopore 2 Capsules with 0.45 µm final filter5441306G5-00-B 0.03 m2 filter area5441306G7-**-B 0.05 m2 filter area5441306G8-**-B 0.1 m2 filter area5441306G9-**A 0.2 m2 filter area5441306G0-** 0.45 m2 filter area

2. Sartopore 2 Capsules with 0.2 µm final filter5441307H4-**-B 0.015 m2 filter area5441307H5-00-B 0.03 m2 filter area5441307H7-**-B 0.05 m2 filter area5441307H8-**-B 0.1 m2 filter area5441307H9-**-A 0.2 m2 filter area5441307H0-** 0.45 m2 filter area

3. Sartopore 2 Capsules with 0.1 µm final filter5441358K4-**-B 0.015 µm filter area5441358K5-00-B 0.03 m2 filter area5441358K7-**-B 0.05 m2 filter area5441358K8-**-B 0.1 m2 filter area5441358K9-**-A 0.2 m2 filter area5441358K0-** 0.45 m2 filter area

* Also available as mini cartridges with the same pore sizes and areas.Order numbers for packs of 5:

areapore size: 0.05 m2 0.1 m2 0.2 m2

0.1 µm 5441558K7B 5441558K8B 5441558K9B0.2 µm 5441507H7B 5441507H8B 5441507H9B0.45 µm 5441506D7B 5441506D8B 5441506D9B

**: available with -SS, -SO, -00 connector

66

0

10

20

30

40

50

60

Sartopore 2 PVDF N66 PES

Tota

l filt

rati

on v

olum

e(k

g fi

ltra

te a

t 90

% b

lock

ing)

Total filtration volume in comparison

10" cartridge formatPES

Type 00 (hose nipple inletand outlet)

Type SS (Sanitary flangeinlet and outlet

Products for ultrafiltration, protein purification and cell culture.

Centrisart® centrifugal units for rapid sample preparation.

Centrisart® I for preparation ofprotein-free ultrafiltrates.Ready-to-use units for proteinbinding studies and for removalof proteins from biological sam-ples, e.g. for determinationof cretanine in human serum.

Centrisart I consists of a sampletube and a free-sliding inner tubewith an ultrafilter bottom. Theprinciple of construction turnsthe traditional way upside down:ultrafiltration takes place in theopposite direction of the cen-trifugal force. This preventsblocking of the ultrafilter andeven allows filtration of particle-containing samples. The ultra-filtrate is collected in the innertube and can be easily removed.

Centrisart® C4 microfiltrationunits for particle removal.Centrisart C4 0,2 µm units aredesigned for centrifugal smallvolume microfiltration. Majorapplications are the removal ofagarose particles from DNA elut-ed from gels and retention ofcells and bacteria.

Specifications for Centrisart IDimensions of the Centrifugal tube, max. outer

diameter, 14 mm. 93 mm long. Can beused in each laboratory centrifuge for14 mm or 17 mm + 100 mm tubes


Materials polystyrene centrifugal tubes. Celluloseproprionate inner tube. Cellulose triace-tate or polysulfone ultrafilter. Polyethylene cap

Max. centrifugal force 2,500 + g (swing bucket rotor) and 2,000 + g (fixed angle rotor)

Sample volume max. 2.5 ml. Min. final volume, 100 µlUltrafiltration rate depends on the protein content of the

sample, the temperature, and on theultrafilter cut-off used. In general, 2.5 mlsample volumes can be ultrafiltered in 30 to 90 minutes.

Order numbers for Centrisart I13209 E Trial pack, with 3 each units of 5,000,

10,000, 20,000 and 100,000 MWCO13229 E with 5,000 MWCO cellulose triacetate

ultrafilter, pack of 1213239 E with 10,000 MWCO cellulose triacetate

ultrafilter, pack of 1213249 E with 20,000 MWCO cellulose triacetate

ultrafilter, pack of 1213269 E with 100,000 MWCO polyethersulfone

ultrafilter, pack of 1213279 E with 300,000 MWCO polyethersulfone

ultrafilter, pack of 12

Specifications for Centrisart C4Maximal capacity 0.4 mlMaximal centrifugal force 10,000 + gFilter area 0.28 cm2

Hold-up volume less than 4 µlMaterials Polypropylene centrifuge tube, cap and

inner tube. Cellulose acetate membranefilter (0.2 µm pore size). Silicone O-ring

Filtration rate Particle removal with 0.2 µm units takes30 minutes at most.

Order number for Centrisart C413207 C4-G 0.2 µm (pack of 25)

68

Direction ofultrafiltration

Particles etc.

Centrifugalforce

Sample

Ultrafilter

Ultrafiltrate

Vivaspin 500Vivaspin 500 centrifugal filterunits offer a simple, one stepprocedure for sample prepara-tion. They can effectively be usedin either swing out or fixed angle rotors accepting 2.2 mlcentrifuge tubes.

The patented vertical membranedesign and thin channel filtrationchamber (US 5,647,990), mini-mises membrane fouling andprovides high speed concentra-tions, even with particle ladensolutions.

Vivaspin 2The Vivaspin 2 bridges the gapbetween the 500 µl and 6 mlcentrifugal concentrators. Thisdevice combines the speed of theclassic Vivaspin products withlow internal surface and mem-brane area for superior recoveriesfrom very dilute solutions.

Available with a choice of PES, Cellulose Triacetate and Regener-ated Cellulose membranes,Vivaspin 2 offers the highestflexibility for process optimisa-tion.

Vivaspin 6 and Vivaspin 20The Vivaspin 6 and 20 centrifugal concentrators have been devel-oped to offer increased volumeflexibility and performance.

Vivaspin 6 can process a record6 ml in either swing bucket orfixed angle rotors acceptingstandard 15 ml conical bottomtest tubes.

Vivaspin 20 handles up to 20 mlin swing bucket centrifuges,14 ml in 25° fixed angle rotorsaccepting 50 ml centrifuge tubes.

For further flexibility, theVivaspin 20 can be used as a gaspressurised device.

The Vivaspin range of centrifugal concentrators for volumes from 500 µl to 20 ml.

69

Concentration | Purification

Ordering informationVivaspin 500 (100–500 µl samples)Polyethersulfone Membrane, pack of 25

Prod. No.3,000 MWCO VS01915,000 MWCO VS011110,000 MWCO VS010130,000 MWCO VS012150,000 MWCO VS0131100,000 MWCO VS0141300,000 MWCO VS01510.2 µm VS0171

Vivaspin 2 (0.4–2 ml samples)Polyethersulfone Membrane, pack of 25

3,000 MWCO VS02915,000 MWCO VS021110,000 MWCO VS020130,000 MWCO VS022150,000 MWCO VS0231100,000 MWCO VS02410.2 µm VS0271Start pack (5 of each MWCO) VS0251

Vivaspin 2 (0.4–2 ml samples)Regenerated Cellulose Membrane, pack of 25

Prod. No.10,000 MWCO VS02K130,000 MWCO VS02L1100,000 MWCO VS02M1

Vivaspin 2 (0.4–2 ml samples)Cellulose Triacetate Membrane, pack of 25

5,000 MWCO VS02U110,000 MWCO VS02V120,000 MWCO VS02X1

Vivaspin 6 (2–6 ml samples)Polyethersulfone Membrane, pack of 25

5,000 MWCO VS061110,000 MWCO VS060130,000 MWCO VS062150,000 MWCO VS0631100,000 MWCO VS06410.2 µm VS0671Start Pack. VS06S1

Vivaspin 20 (5–20 ml samples)Polyethersulfone Membrane, pack of 12

Prod. No.3,000 MWCO VS20915,000 MWCO VS201110,000 MWCO VS200130,000 MWCO VS202150,000 MWCO VS2031100,000 MWCO VS2041300,000 MWCO VS20511,000,000 MWCO VS20610.2 µm VS2071Start Pack. VS20S1(2 of each 5 K, 10 K, 30 K, 50 K, 100 K, 0.2 µm)

Packs of 100 (VS500, VS2, VS6) and packs of 48 (VS20) are available (see special catalogue).


The Vivacell range of gas pressure concentrators for volumes from 10 ml to 250 ml.

Vivacell 70Vivacell 70 combines the ease ofuse of centrifugal devices withthe flexibility and control provid-ed by pressurised ultrafiltrationcells. Vivacell 70 is inexpensive,quick and easy to assemble,requires no tubing connectionsor stirring mechanisms and can be adapted to equipmentavailability or to specific userpreferences.

The longitudinal membraneinhibits fouling, whilst the built-in dead stop will hinder furtherconcentration when residualvolume drops below 150 µl.

Vivacell 100Vivacell 100 is the latest memberof the Vivacell family and bridgesthe volume range between theVivacell 70 and the Vivacell 250.

The patented vertical membranedesign allows highest perform-ance and unmatched flexibility.

Vivacell 100 is a unique andinnovative concentrator forvolumes from 20 ml to 100 ml,which utilizes Pressure, Centri-fuge, Shaking or Pressure-Shaketo rapidly concentrate evensamples with very high particleloading.

Vivacell 100 is designed forcentrifugal concentration ofsamples up to 100 ml whichmakes it the largest centrifugalunit available. At the same time,the new construction designallows for maximum centrifugalforce of 4.000 + g to be used foreven faster concentration.

Vivacell 250The Vivacell 250 is a totally newconcept for the concentration oflarger biological samples. Thisproduct offers numerous advan-tages when compared to stirredcells.

The Vivacell 250 handles a vol-ume range from under 50 ml to250 ml. Use free standing on abench top or in a refrigerator formaximum simplicity, or use onlaboratory shaker for fastestconcentrations.

The unique conical dead stopbuilt into the bottom of themembrane insert allows concen-trations to under 1 ml.

The gentle vortex action controlsmembrane polarisation whilstgreatly reducing the shear effectstypical of stirring mechanisms.

70

Ordering informationVivacell 70 (10–70 ml samples)Includes Polycarbonae filtrate bottles,pack of 2

Prod. No.5,000 MWCO PES VS601110,000 MWCO PES VS600130,000 MWCO PES VS602150,000 MWCO PES VS6031100,000 MWCO PES VS60410.2 µm PES VS6071

Vivacell 100 (10–100 ml samples)Includes Polycarbonae filtrate bottles,pack of 2

Prod. No.5,000 MWCO PES VC101110,000 MWCO PES VC100130,000 MWCO PES VC102150,000 MWCO PES VC1031100,000 MWCO PES VC1041300,000 MWCO PES VC10511,000,000 MWCO PES VC10610.2 µm VC1071

Vivacell 250 (50–250 ml samples)Prod. No.

Vivacell 250, complete with accessories VCA250Vivacell 250 Membrane Inserts, pack of 5

5,000 MWCO PES VC251110,000 MWCO PES VC250130,000 MWCO PES VC252150,000 MWCO PES VC2531100,000 MWCO PES VC2541Starter Kit (One of each membrane MWCO) VC2551

For larger packs and list of accessories see special cataloque.

Vivaflow 50The novel Vivaflow system(patents pending) provides astandard of ease of use, perform-ance, flexibility and economywhich is unrivalled by any labo-ratory or pilot scale filtrationsystem on the market.

Thin channel flip-flow recircula-tion path provides high crossflow velocities with minimumpump requirements. UniqueInterlocking modules with seriesconnectors for easy scale up.

A single 50 cm2 module will typi-cally reduce 500 ml to less than15 ml in under 50 minutes. Lessthan 500 µl non recoverable holdup volume.

Vivaflow 200Concentrate 250 ml to under20 ml in just a few minutes orconcentrate one litre 50 times inless than 30 minutes. Alternative-ly, use two Vivaflow 200 inparallel and concentrate 5 litresin under 75 minutes.

Near total sample recoveries canbe expected with most solutions. Desalting or buffer exchange isjust as quick when using theoptional recirculation assemblyavailable from Vivascience.

The economical standard packagecomes complete with tubing,pressure control gauge, flowrestrictor and high pressurepump tubing. All you need is a peristaltic pump capable of handling 6.4 mm OD (size 16)tubing.

The Vivaflow range of tangential flow concentrators for volumes from 500 ml to over 5 liters.

71

Concentration | Purification

Ordering informationVivaFlow 50 (pack of 2)Includes size 16 tygon peristaltic tubing,Luer fittings, filtrate tube and flow restrictor

Prod. No.5,000 MWCO PES VF05P110,000 MWCO PES VF05P030,000 MWCO PES VF05P250,000 MWCO PES VF05P3100,000 MWCO PES VF05P40.2 µm PES VF05P710,000 MWCO RC VF05C030,000 MWCO RC VF05C2100,000 MWCO RC VF05C4

For accessories see special catalogue.

VivaFlow 20 (1 unit)Includes pressure indicator, flow restrictor,size 16 tygon persitaltic tubing and fittings

Prod. No.5,000 MWCO PES VF20P110,000 MWCO PES VF20P030,000 MWCO PES VF20P250,000 MWCO PES VF20P3100,000 MWCO PES VF20P40.2 µm (PES) VF20P710,000 MWCO (RC) VF20C030,000 MWCO (RC) VF20C2100,000 MWCO (RC) VF20C410,000 MWCO (Hydrosart®) VF20H030,000 MWCO (Hydrosart®) VF20H2

miniPERM | Lab Scale Protein Production.

A step forward in cell cultureminiPERM is an economical easy-to-use bioreactor which simpli-fies high density cultivation ofhybridoma and other cell types.The main focus is laid on costefficient production of anti-bodies and other desired extra-cellular products in high concen-tration.

miniPERM is suitable for cultiva-tion of a variety of cell types.Suspension cells and anchoragedependent cells can be cultivatedin the miniPERM. Cells densitiesgreater than 107 cells/ml andproduct concentrations of severalmg/ml can be achieved.

ApplicationsminiPERM is appropriate for usein a wide spectrum of researchand laboratory applications:• Hybridoma• Transfected cells• Production of viruses• Insect cells• Plant cells• Production of biomass

miniPERM is available in twoversions: • single-use bioreactor,

completely assembled andsterilized

• reusable nutrient module andsterile single-use productionmodule

miniPERM constructionminiPERM consists of a produc-tion module (with culture vol-umes of 5, 35 or 50 ml) and anutrient module which serves asa medium reservoir (400 ml).

Basic principleWhen assembled, the modulesare separated by a semi-permeable dialysis membrane.Neither the cells, nor high molec-ular weight products released bythe cells are able to pass throughthe membrane. However, nutri-ents and dissolved gases can dif-fuse from the nutrient moduleinto the production module. Lowmolecular weight metabolitescan leave the production modulevia the dialysis membrane wherethey are diluted in the medium ofthe nutrient module.

The outer side of the productionmodule is sealed with a thin, gas-permeable silicone membrane for an optimal exchange of O2 andCO2.

CultivationIn the miniPERM the cell suspen-sion must be circulated constant-ly, preferably on the UniversalTurning Device. Then cells areoptimally supplied and theirmetabolic waste products areremoved.

72

Ordering Information

Kits Contents miniPERM classic miniPERM SM

miniPERM Kit 12 Production Modules, 4 Nutrient Modules, IV-76001052 IV-760776174 Stands

miniPERM SM Kit 4 miniPERM Bioreactors IV-76001059 IV-76077618Sterile

miniPERM Test Kit 4 Production Modules, 2 Nutrient Modules, IV-76001058 IV-760776191 Start-up Support Kit

miniPERM Test Kit 4 Bioreactors, 1 Start-up Support Kit IV-76077009 IV-76077609Sterile

Production module 1 Pack (12 units) IV-76001055 IV-76077616


Cell culture vessel for parallelinvestigations

The rectangular quadriPERM cellculture vessel with its four sub-divisions, offers space for slideswith universally recogniseddimensions (26 + 76 mm). Cellscan be grown simultaneously inthe four separate compartmentsunder identical conditions, eitheron coated or un-coated slides or without slides.

ApplicationsCells can be fixed, stained andimmunohistologically tagged.Cells grown on slides inquadriPERM can be used for allmolecular biological andimmunological detectionmethods.

• In-situ preparation of amniotic cells

• Chromosome analysis• Micronucleus assay • Replication studies• Cytoskeleton staining• Immunfluorescence tests

(FISH)

flexiPERM – The flexible cell culture chamber

flexiPERM, a reusable cell cultureinsert made of silicone, can becombined with standard micro-scope slides (26 + 76 mm) andpetri dishes, creating multiplegrowth chambers.

flexiPERM is heat and cold resist-ant, hydrophobic and non-toxicto cells. Because of its highlyadhesive underside, it sticks to allsmooth surfaces, e.g. glass, plastic and foil.

flexiPERM slide (1) with 8 and flexiPERM micro 12 (2) with12 subdivisions, are perfect forparallel cell culture studies onDIN standard microscope slides.flexiPERM disc (3) splits every50 mm diameter petri dish (e.g.petriPERM) into four separatecell culture chambers.

Cell culture dish with gas-permeable base

petriPERM is a cell culture dishwith a gas-permeable base madeof a 25 µm thick plastic foil(bioFOLIE). The cells grow directlyon the border between gas andliquid phase, where the culturemedium does not act as a diffu-sion barrier. Therefore an optimaltransfer of CO2 and O2 is guaran-teed.

Optimal growth conditionspetriPERM is available with adiameter of 50 and 35 mm. The user can choose between ahydrophilic or a hydrophobicculture surface. Therefore, bothadherent and suspension cellscan be cultivated on petriPERM.

For sensitive cells we offerpetriPERM 50 in tissue culture(TC) quality with a hydrophilicculture surface which guaranteeshigh adherence for cells.


Pack Size Cat. No.

quadriPERM 192, (16 + 12), sterile IV-76077308

flexiPERM micro 12 5, reusable IV-50011436

flexiPERM slide 5, reusable IV-50032039

flexiPERM disc 5, reusable IV-50034067

petriPERM 35 hydrophilic 50, sterile IV-76077331

petriPERM 35 hydrophobic 50, sterile IV-76077333

petriPERM 50 hydrophilic 50, sterile IV-76077303

petriPERM 50 hydrophobic 50, sterile IV-76077306

petriPERM 50 TC hydrophilic 50, sterile IV-76077410

quadriPERM, flexiPERM, petriPERM.

73

Cell Culture

(1)

(2)

(3)

Sartocon Slice cassettes have thesame materials and the sameconstruction and therefore alsothe identical flow path length asthe Sartocon cassettes, used forlarger production scale. Thescale-up and the scale-down isperfectly linear throughout therange of applications, from pro-tein purification and concentra-tion to cell harvesting and bloodfiltration. Validation require-ments, and their high costs, aregreatly reduced or entirely elimi-nated.

All cassettes have an excellentchemical compatibility, coveringa wide pH-range. Depending onthe MWCO, they are autoclavableor in-line steamable, so that theycan be easily and efficientlycleaned and sterilized prior to re-use.

The Sartocon Slice holdingsystem can accommodate up tothree Sartocon Slice cassettes. It is designed for maximum per-formance and ease of cleaning.The construction with all processconnectors on a stationary plateallows the fixed tubing of theholder and an effective cleaning.Sartoflow alpha is an optimisedultrafiltration system includingpump with optional data record-ing.

Specifications for Sartocon Slice Cassettes:Biosafety All materials pass the USP Plastics Test

Class VI.Chemical compatibility pH 2–14 (Hydrosart), pH 1–14

(polyethersulfone)Filter area 0.1 m2

Application limits Max. 4 bar inlet pressure. Max. 50°C operating temperature

Order numbers for the Sartocon Slice holding system:17521-001 Sartocon Slice holding device

(without accessories)17521-101 Sartocon Slice Set with accessories

for microfiltration17521-102 Sartocon Slice Set with accessories

for ultrafiltration

Order numbers for the Sartocon Slice Cassettes:3051860601W--SG Hydrosart, 0.45 µm pore size3051860701W--SG Hydrosart, 0.2 µm pore size3051860601O--SG Hydrosart, 0.45 µm open channel3051860701O--SG Hydrosart, 0.2 µm open channel

3051467901E--SG Polyethersulfone, 300,000 MWCO3051466801E--SG Polyethersulfone, 100,000 MWCO3051465001E--SG Polyethersulfone, 50,000 MWCO3051465901E--SG Polyethersulfone, 30,000 MWCO302146AL01K--SG PESU max. for albumin3051463401E--SG Polyethersulfone, 8,000 MWCO3051463901E--SG Polyethersulfone, 10,000 MWCO3051462901E--SG Polyethersulfone, 5,000 MWCO3051460901E--SG Polyethersulfone, 1,000 MWCO3051442901E--SG Hydrosart, 5,000 MWCO3051443901E--SG Hydrosart, 10,000 MWCO3051445901E--SG Hydrosart, 30,000 MWCO

Sartocon® Slice. The pilot-scale crossflow system for batches of 1 to 100 litres.

74

75

Crossflow Filtration

The Vivapure range of ion exchange spin columns for rapid protein separation and purification.

New Vivapure spin columnsemploy an innovative and power-ful ion exchange membranetechnology for purification ofproteins. Vivapure purificationprotocols allow the isolation ofultrapure proteins with high yieldin less than 30 minutes.

Separation of pure proteins fromcomplex mixtures is a key processin biomedical research and otherbiological disciplines. One of themost widely used techniques toseparate proteins is ion exchangechromatography, but this typi-cally involves lengthy proceduresand expensive equipment.

Vivapure centrifugal ionexchange membrane spincolumns will dramatically changethe way you isolate and purifyproteins.

RapidVivapure replaces time-consum-ing, tedious and expensivemethods such as HPLC and FPLC®

for many protein applications.The rapid purification protocolbased on membrane adsorbersallows the isolation of ultrapureprotein with high yield in lessthan 30 minutes. Ion exchangeseparation is the most widelyused technique for chromato-graphic separation of proteins.

User friendlyVivapure spin columns are avail-able with strong or weak cationor anion charged membranematrices. With these ionexchange membrane devices,protein binding, elution andconsequent purification andconcentration are made almostas simple as filtration.

High purity sampleThe protein sample purified usingthe Vivapure spin column can beused in downstream applicationsincluding SDS-PAGE gel elec-trophoresis, Western blotting,iso-electric focusing, X-ray crys-tallisation, NMR spectroscopy,MALDI-TOF mass spectrometryand related proteomics applica-tions.

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Vivapure Mini (up to 0.5 ml), pack of 24

VS-IX01CH24 Carboxyl, weak cation

VS-IX01DH24 Diethylamine, weak anion

VS-IX01QH24 Quaternary ammonium, strong anion

VS-IX01SH24 Sulfonic acid, strong cation

VS-IX01ST16 Starter kit (4 each)

Vivapure Maxi (up to 20 ml), pack of 8

VS-IX20CH08 Carboxyl, weak cation

VS-IX20DH08 Diethylamine, weak anion

VS-IX20QH08 Quaternary ammonium, strong anion

VS-IX20SH08 Sulfonic acid, strong cation

Vivapure Mini are available in high (H), medium (M) and low (L),Vivapure Maxi in high (H) and medium (M) protein binding capacity.For detailed information see special brochure.

Ultrasart D20 for undisturbed LAL tests.

Ultrasart D20The Limulus Amoebocyte Lysatetest is commonly used in thepharmaceutical quality control.The ready-to-use ultrafiltrationunits Ultrasart D20 allow theremoval of disturbing, low-molecular substances out of LAL-test samples within15–30 minutes, without reducingthe sensitivity of the test.

Accessories

Pressure system for Ultrasart D20Order number: 16506Consists of a pressure manifoldfor 3 Ultrasart D20 units, taps forindividual control of pressureand/or flow and air venting, a3 litre pressure tank and con-necting hoses. Additional pres-sure manifolds can be connectedby using an adapter 17152 or17153.

Depyrogenation, after removal of the pressure gauge, at up to200°C.

Max. operating pressure, 5 bar.

SpecificationsChemical compatibility resistant to aqueous solutions of pH 3–9,

and when contacting 1M amino acid up to2 hours


Flow rate for water at 1 bar, 2 ml/minMaterials Cellulose triacetate ultrafilter (20,000 D

MWCO, 100% endotoxin retention), SANand MBS-cyrolite housing

Max. sample volume 15 ml

Order numbers16520 C Ultrasart D20 ultrafiltration units, sterile

and pyrogen-free (pack of 6)

76

Membrane Filters andNutrient Pad Sets for use in analysis, researchand control.

Low adsorption Cellulose Acetate membranes, type 111, for the filtration of aqueous solutions.

Cellulose acetate membranescombine high flow rates andthermal stability with very lowadsorption characteristics, andare therefore excellently suitedfor use in pressure filtrationdevices.The membrane with 0.2 µm is the filter of choice forsterile filtration of aqueous solutions, such as nutrient media,buffers and sera.

The results of publications onadsorption are difficult to correlate, as mostly different testsubstances, conditions and detec-tion methods were used, and themembranes were tested withoutpreviously being sterilized. The graph shown below presentsresults of our application labora-tories. 0.2 membranes of differentmaterials were first autoclaved, in order to simulate normal usageconditions. The adsorption(µg/cm2) was then measured.

Specifications for Cellulose Acetate membrane filtersAdsorption see graph, bottom leftExtractables with water less than 1%Autoclaving at 121°C or 134°CBubble Point minimum value for acc. DIN 58355 0.2 µm = 3.3 bar (330 kPa),

for 0.45 µm = 1.9 bar (190 kPa), for 0.65 µm = 1.3 bar (130 kPa), for 0.8 µm = 0.8 bar (80 kPa)

Chemical compatibility resistant to aqueous solutions, pH 4–8,against most alcohols, hydrocarbons and oils

Thickness average value 120 µmFlow rate for water average value per cm2 area at ∆p = 1 bar

(100 kPa): 25 ml/min for 0.2 µm, 69 ml/min for 0.45 µm, 130 ml/min for0.65 µm, 200 ml/min for 0.8 µm pore size

Material cellulose acetateSterilizing filtration filters with 0.2 µm pore sizes are validated

by Bacteria Challenge Tests.Sterilization by autoclaving, with γ-radiation, dry heat

or ethylene oxideThermal stability max. 180°C

Order Numbers for Cellulose Acetate membrane filters

13 mm diameter:11104-013N 0.8 µm, pack of 10011106-013N 0.45 µm, pack of 10011107-013N 0.2 µm, pack of 100

25 mm diameter:11104-025N 0.8 µm, pack of 10011105-025N 0.65 µm, pack of 10011106-025N 0.45 µm, pack of 10011107-025N 0.2 µm, pack of 100

30 mm diameter:11106-030N 0.45 µm, pack of 10011107-030N 0.2 µm, pack of 100

47 mm diameter:11104-047N 0.8 µm, pack of 10011105-047N 0.65 µm, pack of 10011106-047N 0.45 µm, pack of 10011107-047N 0.2 µm, pack of 100

50 mm diameter:11104-050N 0.8 µm, pack of 10011105-050N 0.65 µm, pack of 10011106-050N 0.45 µm, pack of 10011107-050N 0.2 µm, pack of 10011107-050ACN 0.2 µm, pack of100individually, sterile packed

85 mm diameter:11106-085N 0.45 µm, pack of 100

90 mm diameter:11106-090G 0.45 µm, pack of 2511107-090G 0.2 µm, pack of 25

100 mm diameter:11106-100G 0.45 µm, pack of 2511106-100N 0.45 µm, pack of 10011107-100G 0.2 µm, pack of 2511107-100N 0.2 µm, pack of 100

78

0

10

20

30

BSA (µm/cm ) IgG (µm/cm )2 2

40

139.6

1 2 3 4 1 2 3 4

1 = Nylon 3 = PVDF2 = SFCA 4 = PES

142 mm diameter:11104-142 G 0.8 µm, pack of 2511104-142 N 0.8 µm, pack of 10011105-142 G 0.65 µm, pack of 2511106-142 G 0.45 µm, pack of 2511106-142 N 0.45 µm, pack of 10011107-142 G 0.2 µm, pack of 2511107-142 N 0.2 µm, pack of 100

293 mm diameter:11104-293 G 0.8 µm, pack of 2511104-293 N 0.8 µm, pack of 10011105-293 G 0.65 µm, pack of 2511106-293 G 0.45 µm, pack of 2511106-293 N 0.45 µm, pack of 10011107-293 G 0.2 µm, pack of 2511107-293 N 0.2 µm, pack of 100

These solvent-resistant,hydrophilic membrane filters areexcellently suited for their majorapplication, particle removalfrom solvents.

They are compatible with: Acetone HexaneAcetonitrile IsobutanolGasoline Isopropanoln-Butanol MethyleneCellosolve (ethyl) Methylene

chlorideChloroform Methyl ethyl

ketoneDiethyl acetamide PentaneDimethylsulfoxide Tetrahydro-

furanDioxane TolueneAcetic acid (96%) Trichloro-

aceticacid (25%)

Ethanol Trichlor-ethane

Ethyl acetate WaterEthylene glycol XyleneFreon TF

The 50 mm diameter, 0.45 µmpore size filter, for example, isstandardly used to ultraclean andde-gas solvents and mobile phas-es for HPLC, in combination withthe All-Glass holder described onpage 29.

Regenerated cellulose membranesalso feature low non-specificadsorption.

Specifications for Regenerated Cellulose membranesAdsorption Ca. 24 µg/cm2 for 0.2 µm pore size,

18 µg/cm2 for 0.45 µm pore sizeExtractables With water, less than 1%Bubble-Point Min. values, wetted with water, 4.4 bar acc. DIN 58355 (440 kPa) for 0.2 µm, 2.8 bar (280 kPa)

for 0.45 µmChemical compatibility Resistant to almost all solvents (see table

below left) and against aqueous solutionsin the pH-range 3–12. Further details on p. 107

Thickness Average value 180 µmFlow rate Average value per cm2 area for water at

1 bar (100 kPa) pressure, 15 ml/min for 0.2 µm, 28 ml/minfor 0.45 µm pore size

Material Regenerated cellulose, reinforced with non-woven cellulose

Sterilization By autoclaving (at 121°C or 134°C),Dry heat (180°C), and gamma radiation (25 kGy) or with ethylene oxide

Validation The correlation of the bubble point valuesof the membranes of 0.2 µm pore size tothe reliability of sterilizing filtration hasbeen validated by standard Bacteria Challenge Tests.

Order Numbers for regenerated cellulose membranes

13 mm diameter:18406-013N 0.45 µm (pack of 100)18407-013N 0.2 µm (pack of 100)

25 mm diameter:18407-025N 0.2 µm (pack of 100)

47 mm diameter:18406-047N 0.45 µm (pack of 100)18407-047N 0.2 µm (pack of 100)

50 mm diameter:18407-050N 0.2 µm (pack of 100)

100 mm diameter:18406-100G 0.45 µm (pack of 25)

142 mm diameter:18406-142G 0.45 µm (pack of 25)18407-142G 0.2 µm (pack of 25)18407-142N 0.2 µm (pack of 100)

293 mm diameter:18406-293G 0.45 µm (pack of 25)18407-293G 0.2 µm (pack of 25)

Chemical resistant RC-membranes, type 184, for the filtration of organic solvents.

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Membrane filters

Polyethersulfone membrane filter, type 154,for the filtration of aqueous and aggressive solutions.

The new polyethersulfone mem-brane filters have excellent flowspeeds and, connected to it, a high filterable volume.

Biologic and pharmaceutic solu-tions can be filtered, in the widepH-range of pH 2-12, because oftheir low protein adsorption.

Furthermore, the membranes arevery well suitable for samples ofthe environmental sector.

The filters with 0.1 µm are usedfor the ultracleaning of solutions,e.g. in case of nephelometry.

Specifications for Polyethersulfone Membrane FiltersAdsorption 10 µg/cm2 for IgG, 5 µm/cm2 for BSA,

1.9 µg/cm2 for insulinExtractables with water less than 0.2 %Autoclavable at 121°C or 134°CBubble point acc. 0.1 µm with Isopropanol/water (60/40) DIN 58355 >2.1 bar

0.2 µm = 3.2 bar (320 kPa)0.45 µm = 2.3 bar

Chemical compatibility resistant to some solutions and aggressive.aqueous solutions, pH 2–12

Thickness average value 140 µmFlow rate for water average value per cm2 area at

∆P = 1 bar (100 kPa): 0.1 µm – > 7 ml/min.0.2 µm – >28 ml/min.0.45 µm – >32 ml/min.

Material PolyethersulfoneSterilization capacity filters with 0.2 µm pore sizes have been

validated with the Bacteria Challenge TestSterilization by autoclaving, gamma radiation or with

ethylenoxide

Order Numbers for Polyethersulfone, Type 15425 mm diameter: 15458-025N 0.1 µm (pack of 100)15407-025MIN 0.2 µm (pack of 100)15406-025N 0.45 µm (pack of 100)

47 mm diameter:15458-047N 0.1 µm (pack of 100)15407-047MIN 0.2 µm (pack of 100)15406-047N 0.45 µm (pack of 100)

50 mm diameter:15458-050N 0.1 µm (pack of 100)15407-050MIN 0.2 µm (pack of 100)15406-050N 0.45 µm (pack of 100)

80

Cellulose nitrate is a standardmaterial for membrane filtersand offers a wide range of poresizes from 8 µm to 0.45 µm. The larger pore sizes (8 µm, 5 µm, 3 µm) find use for chemotaxisand cell retention, the 0.45 µmpore size for particle collection.

The high non-specific adsorptionof the cellulose nitrate mem-brane is very advantageous fordiagnostic kits. The adsorption decreases withincreasing pore size, as shown inthe diagram below.

Adsorption (µm g-Globulin/cm2)

Specifications for cellulose nitrate (ester) membrane filtersAdsorption see diagram, bottom leftExtractables with water less than 1%Sterilization by autoclaving, at 121°CBubble Point wetted with water, minimum values:acc. DIN 58355 0.3 bar (30 kPa) for 8 µm pore size, 11301

0.5 bar (50 kPa) for 5 µm pore size, 113420.6 bar (60 kPa) for 3 µm pore size, 113021.0 bar (100 kPa) for 1.2 µm pore size, 113031.4 bar (140 kPa) for 0.8 µm pore size, 113042.0 bar (200 kPa) for 0.65 µm pore size, 113052.4 bar (240 kPa) for 0.45 µm pore size, 11306

Chemical compatibility resistant to aqueous solutions in the pH-range 4-8 to hydrocarbons and to somesolvents

Thickness 130 µm Flow rate for water average values per cm2 area at ∆p = 1 bar

(100 kPa):750 ml/min for 8 µm pore size, 11301570 ml/min for 5 µm pore size, 11342430 ml/min for 3 µm pore size, 11302320 ml/min for 1.2 µm pore size, 11303200 ml/min for 0.8 µm pore size, 11304130 ml/min for 0.65 µm pore size , 1130569 ml/min for 0.45 µm pore size, 11306

Material Cellulose nitrateSterilization by autoclaving, γ-radiation (25 kGy) or with

ethylene oxideThermal stability max. temperature 130°C

Order numbers for cellulose nitrate membrane filters see next page

81

Membrane filters

Cellulose Nitrate membrane (ester) filters, type 113, for sample pretreatment, particle testing and chemotaxis.

Order numbers for cellulose nitrate membrane filters type 113

13 mm diameter:11301-013N 8 µm, pack of 10011342-013N 5 µm, pack of 10011302-013N 3 µm, pack of 10011304-013N 0.8 µm, pack of 10011306-013N 0.45 µm, pack of 100


25 mm diameter:11301-025N 8 µm, pack of 10011342-025N 5 µm, pack of 10011302-025N 3 µm, pack of 10011303-025N 1.2 µm, pack of 10011304-025N 0.8 µm, pack of 10011305-025N 0.65 µm, pack of 10011306-025N 0.45 µm, pack of 100


37 mm diameter:11301-037N 8 µm, pack of 10011304-037N 0.8 µm, pack of 10011306-037N 0.45 µm, pack of 100



80 mm diameter:11301-080ALN 8 µm, pack of 100sterile, non-individually packed


90 mm diameter:11342-090G 5 µm, pack of 2511303-090G 1.2 µm, pack of 2511304-090G 0.8 µm, pack of 2511306-090G 0.45 µm, pack of 2511306-090N 0.45 µm, pack of 100

142 mm diameter:11301-142G 8 µm, pack of 2511302-142G 3 µm, pack of 2511303-142G 1.2 µm, pack of 2511304-142G 0.8 µm, pack of 2511304-142N 0.8 µm, pack of 10011305-142G 0.65 µm, pack of 2511306-142G 0.45 µm, pack of 2511306-142N 0.45 µm, pack of 10011342-142G 5 µm, pack of 2511342-142N 5 µm, pack of 100

293 mm diameter:11301-293G 8 µm, pack of 2511303-293G 1.2 µm, pack of 2511304-293G 0.8 µm, pack of 2511304-293N 0.8 µm, pack of 10011306-293G 0.45 µm, pack of 2511306-293N 0.45 µm, pack of 10011342-293G 5 µm, pack of 25

47 mm and 50 mm filters are, insome pore sizes, sterile, individuallypacked, available in packs of 100.

Order numbers are the following:47 mm diameter:11301-047 ACN 8 µm11302-047 ACN 3 µm11303-047 ACN 1.2 µm11304-047 ACN 0.8 µm11305-047 ACN 0.65 µm11306-047 ACN 0.45 µm

50 mm diameter:11301-050 ACN 8 µm11302-050 ACN 3 µm11303-050 ACN 1.2 µm11304-050 ACN 0.8 µm11305-050 ACN 0.65 µm11306-050 ACN 0.45 µm

82

Polyamide membrane filters arehydrophilic and chemically resist-ant to alkaline solutions andorganic solvents. They are there-fore recommended for particle-removing filtration of water,aqueous solutions and solventsfor analytical determination suchas HPLC, as well as for the sterilefiltration of these liquids.

They are also highly recommend-ed for the isolation of legionella.

Their relatively high non-specificadsorption, which can cause lossof important substances, e.g.from tissue culture solutions,limit their application. For thesekind of solutions, the low adsorp-tion cellulose acetate membranefilters, type 111, described onpage 78, are preferred.

Specifications for polyamide membrane filtersAdsorption 100 µg/cm2 for bovine serum albumin

(0.2 µm pore size)Extractables with water less than 0.2 %Autoclaving at 121°C or 134°CBubble Point minimum value for 0.2 µm = 3.4 bar acc. DIN 58355 (340 kPa),

for 0.45 µm = 2.2 bar (220 kPa).Chemical compatibility resistant to many solvents and alkali-

solutions.pH-range 3–14Thickness average value 125 µmFlow rate for water average value per cm2 area at ∆p = 1 bar

(100 kPa):>12 ml/min for 0.2 µm, >26 ml/min for 0.45 µm pore size

Material PolyamideSterilizing filtration filters with 0.2 µm pore size are validated

by the Bacteria Challenge Test.Sterilization by autoclaving or with ethylene oxide

Order Numbers for polyamide membrane filters13 mm diameters:25006-013N 0.45 µm, pack of 10025007-013N 0.2 µm, pack of 100







Polyamide membrane filters, type 250,for the filtration of alkaline solutions and organic solvents.

83

Membrane filters

n

Hydrophobic PTFE Membranes,type 118, for the filtraton of air, gases or chemicals.

The main application of thismembrane filter types is theair/gas filtration.They are made purely of PTFE(polytetra-fluorethylene), and are therefore permanentlyhydrophobic. Unlike other(hydrophilic) filter types, they are not wetted by air humidity,allowing unhindered passage of air also at low differentialpressures.

PTFE membrane filters have anexcellent chemical compatibility,so that they are also used for thefiltration of solutions and acids,to which other filter types areresistant. Due to their hydro-phobic characteristics, they mustbe pre-wetted with ethanol or methanol before the filtrationof aqueous media.

Specifications for PTFE membrane filtersAdsorption 8 µg/cm2 for gamma-globulin

(0.2 µm pore size)Extractables with water none detectableAutoclaving at 121°C or 134°CBubble Point minimum value for acc. DIN 58355 0.2 µm = 1.2 bar (120 kPa),

for 0.45 µm = 0.8 bar (80 kPa). Average value for 1.2 µm = 0.45 bar (45 kPa), for 5 µm = 0.1 bar (10 kPa)

Chemical compatibility resistant to almost all chemicalsThickness average values, 65 µm for 0.2 µm and

100 µm for 5 µm pore sizeFlow rate for air average values per cm2 area at

Dp = 0.05 bar (5 kPa): 0.2 l/min for 0.2 µm, 0.3 l/min for 0.45 µm, 1.6 l/min for 1.2 µmand 4 l/min for 5 µm pore size

Material PolytetrafluorethyleneSterilization capacity filters with 0.2 µm pore size are validated

with the Bacteria Challenge Test.Sterilization by autoclaving or with ethylene oxide

Order Numbers for PTFE membrane filters13 mm diameter:11803-013N 1.2 µm, pack of 10011806-013N 0.45 µm, pack of 10011807-013N 0.2 µm, pack of 100

25 mm diameter:11842-025N 5 µm, pack of 10011803-025N 1.2 µm, pack of 10011806-025N 0.45 µm, pack of 10011807-025N 0.2 µm, pack of 100



100 mm diameter:11842-100G 5 µm, pack of 2511803-100G 1.2 µm, pack of 2511806-100G 0.45 µm, pack of 2511807-100G 0.2 µm, pack of 25

142 mm diameter:11842-142G 5 µm, pack of 2511803-142G 1.2 µm, pack of 2511806-142G 0.45 µm, pack of 2511807-142G 0.2 µm, pack of 25


84

n

Polycarbonate Track-Etch-Membranes are manufacturedfrom high grade polycarbonatefilm using track-etch technology.They retain particles on their surfaces. Their capillary porestructure is uniform and precise,with a narrow pore size distribu-tion. Track-etch membranes arean excellent choice for accuratefractionation of particulatesbecause of their precise pore size.In addition, their smooth, flatsurface results in high particulatevisibility.

Track-etch technology offers theuser distinct performance advan-tages when excellent surfacecapture and high sample visibilityare required. Applications: Partic-ulate analysis, epifluorescencemicroscopy, fluid clarification,cytology, cell biology, bioassays,water microbiology, environmen-tal analysis.

Specifications for polycarbonate membrane filtersLow extractablesAutoclaving, at 121° CThermal stability max. temperature 140° CBubble Point minimum value for 0.2 µm = 4.8 bar, acc. DIN 58355 for 0.4 µm 2.5 barChemical compatibility see tableThickness 6 –11 µmFlow rate for water 20 ml/min/cm2 for 0.2 µm,

70 ml/min/cm2 for 0.4 µmPorosity <15 %Material polycarbonateSterilization by autoclaving

Order numbersFor polycarbonate membrane filters

25 mm diameter:23007-25 N 0.2 µm, pack of 100, diameter 25 mm23006-25 N 0.4 µm, pack of 100, diameter 25 mm

47 mm diameter:23006-47 N 0.4 µm, pack of 100, diameter 47 mm23007-47 N 0.2 µm, pack of 100, diameter 47 mm

Polycarbonate Track-Etch-Membranes, type 230, for the analysis of particles.

85

Membrane filters

Glass fibre prefilters for larger totally filterable volumesin clarification and sterile filtration.

The major use of all three glassfibre filters is as a depth prefilter,placed directly on top of a mem-brane filter, whereby the prefilterdiameter specified for the holdermust be chosen. Larger diameterswould intrude under the sealingring of the holder and causeleakage.

The standard type 13400 con-tains an acrylic latex binder. It has a high particle loadingcapacity, but for very “dirty” liquids the thicker type 13430can be more effective. 13440 is afiner, binder-free type, and isrecommended for the prefiltra-tion of relative clean solutionssuch as tissue culture media.

Serial filtration may be necessaryfor difficult-to-filter liquids suchas serum. 2 or 3 membrane filters of different pore sizes are placedon each other, with a glass fibreprefilter on top and polyesterseparators 13420 between them(diameter as for prefilter) toassist liquid passage.

SpecificationsAutoclaving 121°C or 134°CThickness ca. 0.55 mm for 13400Flow rates for water at ∆p = 1 bar (100 kPa), 320 ml/min/cm2

for 13400Materials 13400, glass fibre with acrylic latex binder.Max. temperature 220°C for 13400Sterilization by dry heat, at 180°C

Order Numbers

a) Type 13400. Standard glass fibre filters13400-013S 13 mm (pack of 200)13400-042Q 42 mm (pack of 500)13400-044Q 44 mm (pack of 500)13400-047Q 47 mm (pack of 500)13400-050Q 50 mm (pack of 500)13400-100K 100 mm (pack of 50)13400-120K 120 mm (pack of 50)13400-124K 124 mm (pack of 50)13400-127K 127 mm (pack of 50)13400-130K 130 mm (pack of 50)13400-142K 142 mm (pack of 50)13400-150K 150 mm (pack of 50)13400-257K 257 mm (pack of 50)13400-260K 260 mm (pack of 50)13400-279K 279 mm (pack of 50)13400-293K 293 mm (pack of 50)

b) Type 13430. Extra thick glass fibre filters13430-127K 127 mm (pack of 50)13430-130K 130 mm (pack of 50)13430-142K 142 mm (pack of 50)13430-257K 257 mm (pack of 50)13430-279K 279 mm (pack of 50)13430-293K 293 mm (pack of 50)

c) Type 13440. Binder-free glass fibre filters13440-042Q 42 mm (pack of 500)13440-044Q 44 mm (pack of 500)13440-047Q 47 mm (pack of 500)13440-050Q 50 mm (pack of 500)13440-130K 130 mm (pack of 50)

d) Type 13420. Polyester separators13420-088K 88 mm (pack of 50)

86

For more glass fiber filters and for paper filters a detailed brochure is available.

87

Membrane filters

Gridded Membrane filters, acc. to ISO 7704, ISO 7899-2, ISO 8199, ISO 9108-1, ISO 9308-1 and EN 12780 sterile and individually packed, for colony counts.

Sterile, individually packed filtershave long become standard forroutine microbiological qualitycontrol because of the user bene-fits they offer.

They are pre-sterilized and ready-to-use and save preparatory time.As they are individually packed,they avoid the possibility of con-tamination of remaining filters inopened packs and are GLP con-form, having filter identificationand lot number printed on eachindividual envelope.

The increasing demand on thesefilters requires the constructionof a new packaging machine withultra-modern stamping. Eachmembrane is checked to ensure itis not dammaged in any way, ispositioned correctly with no slippage under the edge seal, hasperfect grid printing and is freefrom particles. Each envelope ischecked for readable lettering.Quality control par excellence!

The membrane filtersAll membranes are made of cellu-lose nitrate, a material whichassures effective retention withhigh flow rates and optimumcolony growth. The printed gridhas a size of 3.1+3.1makes thecounting easier, especially forhigher bacteria counts and formicro colonies, but does notinfluence the growth. The variousfilter colours allow the best con-trast to the colonies (see mem-brane filter types, chosen for thenutrient pad sets, page 89).

Hydrophobic edge membranesare used mainly in the sterilitytesting of solutions containingantibiotics.

Specifications for gridded filtertypes, as for filter type 113 (page 81).

Order Numbersfor sterile, individually packedmembrane filters

1. Type 114, white with blackgrid, for bacteriologic research

47 mm diameter,a) In packs of 100:11403-047ACN 1.20 µm11404-047ACN 0.80 µm11405-047ACN 0.65 µm11406-047ACN 0.45 µm11407-047ACN 0.20 µm

b) In packs of 1000:11406-050ACR 0.45 µm11403-047ACR 1.20 µm11404-047ACR 0.80 µm11406-047ACR 0.45 µm

50 mm diameter,a) In packs of 100:11403-050ACN 1.20 µm11404-050ACN 0.80 µm11405-050ACN 0.65 µm11406-050ACN 0.45 µm

b) In packs of 1000:11406-050ACR 0.45 µm11403-050ACR 1.20 µm

2. Type 130, grey (black afterwetting) with white grid, for the detection of yeastsand moulds

47 mm diameter,a) In packs of 100:13004-047ACN 0.80 µm13005-047ACN 0.65 µm13006-047ACN 0.45 µm

b) In packs of 1000:13004-047ACR 0.80 µm13006-047ACR 0.45 µm

50 mm diameter,a) In packs of 100:13004-050ACN 0.80 µm13005-050ACN 0.65 µm13006-050ACN 0.45 µm

b) In packs of 1000:11403-050ACR 1.20 µm13005-050ACR 0.65 µm13006-050ACR 0.45 µm

3. Type 131, white with blackgrid and pink-colouredhydrophobic edge, for sterility testing

3a. With 3 mm edge47 mm diameter,In packs of 100:13106-047ACN 0.45 µm13107-047ACN 0.20 µm

50 mm diameter,In packs of 100:13106-050ACN 0.45 µm13107-050ACN 0.20 µm

3b. With 6 mm edge47 mm diameter,In packs of 100:13106-047HEN 0.45 µm

4. Type 138, green with dark-green grid, for bacteriological research

47 mm diameter,a) In packs of 100:13806-047ACN 0.45 µm

b) In packs of 1000:13806-047ACR 0.45 µm



5. Type 139, white with greengrid, for the research onE.coli and coliform germs

47 mm diameter,a) In packs of 100:13903-047ACN 1.20 µm13906-047ACN 0.45 µm

b) In packs of 1000:13905-047ACN 0.65 µm13906-047ACR 0.45 µm



1. Type 114, white with black grid

25 mm diameter,In packs of 100:11403-025N 1.20 µm11404-025N 0.80 µm11406-025N 0.45 µm

37 mm diameter,In packs of 100:11404-037N 0.80 µm

47 mm diameter,In packs of 100:11403-047N 1.20 µm11404-047N 0.80 µm11405-047N 0.65 µm11406-047N 0.45 µm


2. Type 130, grey (black afterwetting) with white grid

25 mm grid,In packs of 100:13006-025N 0.45 µm13001-025N 8.00 µm



3. a) Type 131, white with blackgrid and 3 mm pink-coloured, hydrophobic edge

25 mm diameter,In packs of 100:13106-025N 0.45 µm13107-025N 0.20 µm



3. b) Type 131, with 6 mmpink-coloured, hydrophobic edge

47 mm diameter, In packs of 100:13106-047-HCN 0.45 µm

4. Type 138, green with dark-green grid

47 mm diameter,a) In packs of 100:13806-047N 0.45 µmb) In packs of 1000:13806-047R 0.45 µm


5. Type 139, white with green grid



6. Type 135, cellulose acetate,white, with hydrophobicedge

47 mm diameter,In packs of 100:13506-47ACN13506-47HCN13506-47N13507-47ACN13507-47N

50 mm diameter,In packs of 100:13506-50ACN

Gridded membrane filter, non-sterile, not individually packed, for particle testing and microcospy

88

6. Type 113, white, no grid, for prefilter holder 16807,page 36

47 mm diameter,In packs of 100:11301-047ACN 8 µm

50 mm diameter,In packs of 100:11301-050ACN 8 µm

7. Dispenserfilter … SCM

47 mm diameter, contains 3 boxes of 100 membraneswhite|grey 11406--47----SCM* 0.45 µmgrey|white13006--47----SCM 0.45 µmgreen|dark green13806--47----SCM 0.45 µm

* 50 mm are available on request

89

Membrane filters

Dessicated media in Petri dishes, with matching membrane filtersfor economic, time-saving microbiologic quality control.

Sartorius Nutrient Pad Sets havebeen used successfully in combi-nation with the membrane filtermethod for more than 20 yearsnow. They ease and simplify a lot of microbiologic researchprocedure, due to their practicalhandling.

One nutrient pad set consists of a50 mm pad, steadily impregnatedwith nutrient media, packed dryand sterile in a Petri dish, plus asuitable membrane filter, testedconcerning colony growth, sterileand individually packed.

Directly after wetting with sterile, demineralised water, thenutrient pad set is ready to use.

Benefits for the user:Economy.No time-consuming and labour-intensive preparation of thenutrient media (sterilization,cleaning, etc.).

Easy handling.Nutrient Pad Sets can also beused in laboratories without acomprising microbiologic equip-ment.

Consistent quality.During the production, eachNutrient Pad Set batch is com-pared with the according agarmedium, in order to guaranteeconsistent quality and repro-ducible results.

Trouble-free storage.Nutrient Pad Sets can be storedat room temperature in a dry,dark warehouse, between 9 and24 months depending on thetype.

Order Numbers for Nutrient Pad Sets in Petri dishesNutrient Pad Determinaton | OrderSet type Detection*: number**:Azide Enterococci (1) 14051-047NBismut sulfite Salmonellae (1) 14057-047NCaso Colony count (1) 14063-047NCetrimide Pseudomonas aeruginosa (2) 14075-047NChapman Staphylococci (2) 14074-047NChromocult E. coli and coliforms (8) 14087-047NECD E. coli (2) 14082-047NEndo E. coli and coliforms (2) 14053-047NGlucose-Tryptone mesophilic bacteria and 14066-047N

thermophilic spore formers (2)Lysine Wild yeasts (3) 14061-047NMalt extract Yeasts and moulds (7) 14086-047NMcConkey Enterobacteria (2) 14097-047NM-FC E. coli and coliforms (2) 14068-047NOrange serum Acid-tolerant microbes (1) 14062-047NOrange serum (pH 3.2) Acid-tolerant microbes (1) 14096-047NR2A Colony count (1) 14084-047NSabouraud Yeasts and moulds (4) 14069-047NSchaufus-Pottinger Yeasts and moulds (5) 14070-047NSchaufus-Pottinger Yeasts and moulds (6) 14072-047NSchaufus-Pottinger Yeasts and moulds (7) 14080-047NSchaufus-Pottinger Yeasts and moulds (4) 14083-047NStandard Colony count (1) 14064-047NStandard TTC Colony count (1) 14055-047NTeepol E. coli und coliforms (2) 14067-047NTergitol TTC E. coli und coliforms (2) 14056-047NTomato juice lactic acid bacteria, special

Leuconostoc oenos (1) 14079-047NVLB-S7-S beer spoiling bacteria (2) 14059-047NWeman Leuconostoc mesenteroides (1) 14065-047NWallerstein lactic acid bacteria and

other beverage spoilers 14089-047NWort Yeasts and moulds (4) 14058-047NWort (pH 4,4) Yeasts and moulds (4) 14078-047NYeast extract colony count (1) 14090-047N

* A pack contains 100 sterile Nutrient Pad Sets, each of them individually packed in a Petri dish, and 100 sterile, individually packed membrane filters.The membrane filters are selected for optimum growth in the interaction with the cor-responding nutrient media. The supplied membrane filter type is listed within brackets:

(1) = green with dark green grid, 0.45 µm pore size(2) = white with green grid, 0.45 µm pore size(3) = green with dark green grid, 0.65 µm pore size(4) = grey (black after wetting) with white grid, 0.65 µm pore size(5) = white with green grid, 0.65 µm pore size(6) = white with green grid, 1.2 µm pore size(7) = grey (black after wetting) with white grid, 0.8 µm pore size(8) = white with black grid, 0.45 µm pore size

** Diameter of the membrane filter, 47 mm. Order number for NutrientPad Set with 50 mm membrane filter as above, but -047N replacedby -050N.

Some Nutrient Pad Sets are available polyethylene bags, without Petri dishes. Each packcontaints 25 bags with 2 Nutrient Pad Sets and 2 membrane filters each, all sterile. Order numbers with 50 membrane filters: Endo, 14003-050K. Standard TTC, 14005-050K.Tergitol TTC, 14006-050K. Wort, 14008-050K. Order numbers with 50 mm membrane fil-ters as for 47 mm but with -050 instead of -047.

Detailed brochure available.

Nutrient Pad Set Poster.

90

The photo shows a poster, original size 70 cm + 50 cm, with growth patternsand typical applications for the Nutrient Pad Sets, described on theprevious page. On request, you can get the poster free of charge.

91

Nutrient Pad Sets

Culture media in tubes ancl bottles. Absorbent Pads.

Culture media for direct incuba-tion are available in two differentforms, in tubes and in bottles.

1. Culture media in tubesFor the casting of plates. The content of one tube is enoughfor two 90 mm or three 60 mmPetri dishes.

Order numbers (50 tubes with 20 ml each):14130K Orange serum agar14131K Standard agar 14135K Malt extract agar14137K Nutrient agar

according to DEV14138K Wort agar 14158K Endo agar14130K Orange serum agar

2. Culture media in bottles

2a. Agar culture media in bottles

Culture media for self-casting informs at very favourable prices.

Order numbers(4 bottles with 250 ml each):14144A Nutrient agar

according to EDV 14148A VLB-S7-S-Agar14156A Endo agar14157A Wort agar14166A Sabouraud agar

2b. Lactose bouillon in bottles

Order numbers 14155A Two times concentrated

(4 Bottles à 100 ml)14160 three times

concentrated (1 Bottle à 1000 ml)

Absorbent pads Our 1.4 mm thick absorbent padsare wetted with the appropriateliquid culture medium before a membrane filter is placed onthem. They come presterilized inplastic tubes, which fit onto theSartorius Manual DispensingDevice, and are available in twodiameters: 47 mm: approx. 3ml absorption

capacity 50 mm: approx. 3.5 ml

absorption capacitiy.

Order numbers (1,000/box, in 10 tubes, each with100 pads, and with manual dis-pensing device, all presterilized)

47 mm d: 15410-047 ALR50 mm d: 15410-050 ALR

Absorbent padsincluding membrane filters and 2 manual dispensing devices.ETO presterilized13906-47 APR

Plastic Petri dishes

Disposable Petri dishes for recep-tion of agar culture media.

Order numbers (pack of 100, sterile): 14311-60N 60 mm diameter14311-90N 90 mm diameter

Instruments for use in laboratories.

arium 613® Reverse Osmosis (RO) System for general laboratory water requirements.

Designed to meet the demands oftoday's laboratories for RO water.

With production volumes of upto 15 liters per hour, 99% rejection of monovalent andpolyvalent ions, bacteria andviruses, automated membraneback flushing, together with it'scompact design the arium® 613 is the ideal choice for generallaboratory applications such asdish washing water, humidifiersand as a pretreatment step priorto ultrapure laboratory watersystems.

Low energylhigh output thin filmcomposite membranes, dramati-cally reduce the volume of waterrejected to waste and increasethe % recovery.

Energy consumption is reducedby the incorporation of a unique-ly designed RO module housingwhich negates the need for solenoid valves to regulate andcontrol permeate flow. The absence of any solenoidvalves not only conserves power,it also reduces maintenance andincreases reliability.

Water storage is simplified by theuse of a closed pressure vessel.This dramatically reduces thecosts of maintenance due to air-borne contamination, while atthe same time reducing gas up-take by product water in storage.A pressurized storage tank pro-vides more flexibility as to itslocation, without the need of anexpensive distribution pump todeliver the water to point of use.

SpecificationsUnit Measurements: 43 cm (17") Width, 48 cm (19") High

33.4 cm (13.5") Depth

System Weight: 14 Kg (30 Ibs.)

Operation Weight. 20.5 Kg (45 Ibs.)

Storage tank [55 I] 67 cm (26.5") High, 41 cm (16") Wide6.8 Kg (15 Ib) Empty, 55 Kg (121 Ib) Full

Inlet Water Requirement: Potable Tap Water Feed Only Feed water to meet drinking water standards of US EPA, European Union orJapan. Max. Total Dissolved Solids (TDS) = 800.0ppm as CaC03Max. Total Hardness = 200.0 ppm as CaC03Iron Maximum 0.1 ppm Turbidity: <1 NTU pH Range 3-11 Temperature Range 2°C -37°C (38°F- 100° F) Minimum Inlet Pressure = 1 bar (15 psi)Maximum Inlet Pressure = 6.8 bar (100 psi)Silting Density Index (SDI) < 5% Langelier Saturation Index (LSI) negative

Operating Specification: Monovalent lon rejection 94-99% Polyvalentions 94-99% Microorganism rejection >99% Particle rejection >99% TOC <100 ppb or >99%for MW >300

Flow Rate: ProductlPermeate up to 15 l/hr @ 25°CRejectlconcentrate up to 12 l/hr Recovery approximately 55%

Electrical: 100–240 VAC, 50/60 HZ single phase

Ambient Operation: 5–28°C, 80% RH

Ambient Storage: 5–45°C, 80% RH

Order Numbers61315055F05M1A Complete RO system including 55 I storage

tank, RO membranes and 2 pretreatmentcartridges.

613CPF05———-V PretreatmentCartridge + 2

613CPM1————-V RO module + 2613CDS2 RO sanitizing syringe + 2611 CDS2 Storage Tank

sanitizing syringe + 2613APV55 55 I storage tank613APV75 75 I storage tank

94

Type 1* laboratory water purifi-cation system. Designed to meetthe requirements of reagentgrade water for routine analysis

* Water exceeds ASTM, NCCLS, ISO and USPreagent water standards.

Advanced Design FeaturesInclude: Carbon-resin Technology A unique carbon-resin combina-tion enables the 611 system toproduce ultrapure water with aresistivity up to 18.2 MO + cm,with Total Organic Carbon (TOC)levels as low as < 1 ppb. Four Line Alphanumeric VDUContinuously indicates waterquality in MO + cm or µS/cmwith compensated or non-com-pensated temperature. Removable Display & DispenseEnables high quality water to beprovided at point of use up totwo meters from the systemsinstallation point. Standby Mode Water recirculates 15 minutesevery hour of inactivity to main-tain purity. Timed (Volume) Dispense Automated dispensing option.Quality Inert materials used for all wetted parts . Security 0.2 µm PESU membrane filtercapsule ensures a bacterial freefiltrate.

SpecificationsUnit Measurements: 43 cm (17") Width, 48 cm (19") High

33.4 cm (13.5 ") Depth

Removable display: 30.2 cm (11.9") Width, 11.3 cm (4.4")High, 15.7 cm (6.2") Depth

System Weight: 14 Kg (30 Ibs.)

Operation Weight: 20.6 kg (45.5 Ib) 611 Dl& 611 UF,23.6 kg (52 Ib) 611 VF

Clearances: Side 17 cm (7") for handlingTop 5 cm (2") for ventilationFront 35 cm (14") for door opening

Inlet Water Requirements Specific ResistivityRO water 20 KO x cm (50µS/cm)Distilled water 250 KO + cm (4µS/cm)Deionized water: 50 KO + cm (20µS/cm)TOC <1000 ppbTurbidity <1 N.T.U.Silica <1000 ppbMinimum inlet pressure: GravityMaximum inlet pressure: 7 bar (103 psi)

Electrical: 100–240 VAC, 50/60 HZ single phase

Ambient Operation: 5–28°C, 80% RH

Ambient Storage: 5–45°C, 80% RH

Interface: RS232 Parallel Port

Product H2O 611DI 611 UV 611UF 611 VFQuality

Resistivity 18.2 18.2 18.2 18.2MO + cm

TQC < 4ppb < 1ppb < 4ppb < 1ppb

Particles < 1/ml < 1/ml < 1/ml < 1/ml@ 0.2 µm

Endotoxin NA NA < 0.001 < 0.001 EU/ml EU/ml

Bacteria <1 CFU/ml <1 CFU/ml <1 CFU/ml <1 CFU/ml

Flow rate up to 2 l/min 2 l/min 1.5 l/min 1.5 l/min

UV light No Yes No Yes

Ultrafilter No No Yes Yes

Order NumbersAs the model and cartridge type is application specific please contactus for a complete data sheet and quotation

arium 611® Laboratory Water Systems for critical reagent grade water requirements.

95

arium

BBI Laboratory Instruments

CERTOMAT® Benchtop Shakers.

CERTOMAT® benchtop shakersfrom B.Braun Biotech Interna-tional have proven their value forlong-term use in microbiology,cell biology, pharmacology andchemistry laboratories world-wide.

Instruments available range from the economicalCERTOMAT® MO ll with simplespeed and time control, over theCERTOMAT® S ll with analogueoutput for data documentationand memory function, to theCERTOMAT® RM with variablemass compensation allowingmaximum speed with maximumload. The CERTOMAT® R andCERTOMAT® U models in addi-tion are extremely silent runnersdue to their strong magneticdrive.

All benchtop shakers can be combined with our incubationhoods CERTOMAT® H or HK inorder to provide a temperaturecontrolled environment.

CERTOMAT® benchtop shakersare available with shaking amplitudes of 12,5 mm, 25 mmor 50 mm and can be run at up to 400 rpm which makes themvery efficient tools for cell culti-vation and general mixing appli-cations.

For more details see our specialbrochures available on requestfrom your local Sartorius office.

AccessoriesCERTOMAT® benchtop shakers are compatible with the full range of accessories:

• Stainless steel trays (type EU, 420 + 420 mm or type FU, 420 + 800 mm )

• Stainless steel or reinforced plastic clamps for Erlenmeyer or Fernbach flasks from 25 ml up to 5 L volume

• Hinged racks for test tubes or centrifuge tubes up to 30 mm diameter

• Universal mounting system with clamping rods for odd-shaped vessels

• Sticky tape or anti-skid layer for easy exchange of flasks of flat-bottomed containers such as microplates

• Holders for microtiter plates

Accessories are not part of the instruments and have to be orderedseparately.

Order NumbersCERTOMAT® MO ll 12.5 mm BBI-8860858

25 mm BBI-8860866CERTOMAT® S ll 25 mm BBI-8862524

50 mm BBI-8862621CERTOMAT® RM 25 mm BBI-8862320

50 mm BBI-8862427CERTOMAT® R 25 mm BBI-8863024CERTOMAT® U 25 mm BBI-8863121CERTOMAT® H heating BBI-8863202CERTOMAT® HK heat | cool BBI-8863245

98

Detailed brochure available.

CERTOMAT® incubation shakersfrom B.Braun Biotech Inter-national provide a temperature-controlled environment for cellcultivation in microbiology, cellbiology and other applicationfields.

Temperature ranges from 8°Cabove ambient up to +70°C, withthe optional integrated coolingbetween 10°C below ambient and+70°C can be achieved. All unitshave shaking orbits of 25 or 50 mm and can be run up to 400 rpm.

CERTOMAT® incubation shakersare fully programmable for allparameters: up to 5 programswith 4 steps each can be storedpassword protected. Safety features include the memoryfunction that stores the last setpoints and installs them afterpower failure, and a stainlesssteel spill tray. Continuousrecording of all parameters ispossible by analogue output.

The CERTOMAT® IS is a benchtopmodel with small footprint of540 + 680 mm and can be usedfor flasks up to 3 L volume.

The CERTOMAT® BS-1 is a largecapacity unit for up to six 5 Lflasks. Illumination units for pho-tosynthetic applications areoptional. Three CERTOMAT® BS-1units can be stacked withoutadditional equipment and runindependently.

The CERTOMAT® BS-T is a top-loading incubator-shaker withthe same features as describedabove for the BS-1, including aninterior made of polished stain-less steel (1.4301) for easy clean-ing and sanitizing.

For more details see our specialbrochures available on requestfrom your local Sartorius office.

AccessoriesCERTOMAT® benchtop shakers are compatible with the full range of accessories:

• Stainless steel trays (type EU, 420 + 420 mm or type FU, 420 + 800 mm )

• Stainless steel or reinforced plastic clamps forErlenmeyer or Fernbach flasks from 25 ml up to 5 L volume

• Hinged racks for test tubes or centrifuge tubes up to 30 mm diameter

• Universal mounting system with clamping rods for odd-shaped vessels

• Sticky tape or anti-skid layer for easy exchange of flasks or flat-bottomed containers such as microplates

• Holders for microtiter plates• Illumination units, 6 + 18 W fluorescent tubes

Accessories are not part of the instruments and have to be orderedseparately.

Order NumbersCERTOMAT® IS 25 mm heating BBI-8864829

heat|cool BBI-886484550 mm heating BBI-8864926

heat|cool BBI-8864942CERTOMAT® BS-1 25 mm heating BBI-8865027


heat|cool BBI-8865329CERTOMAT® BS-T 25 mm heating BBI-8865426


heat|cool BBI-8865728

CERTOMAT® Incubation Shakers.

99


Homogenizers.

Laboratory work often requiresthat a sample is prepared for subsequent analysis by homo-genization. This may involve justmixing of liquids, but more oftenit means destroying the structureof biological material so thatsubstances become accessible forisolation and analysis. Dependingon the sample material, therequired volume and the intend-ed analysis of the homogenates,samples have to be treated withdifferent homogenizers. For thisreason B. Braun Biotech Interna-tional offers a wide range ofhomogenizers for different appli-cations.

The ball mill Mikro-DismembratorU is very popular among scientistswho have to disrupt brittle orfrozen material. For operation ashaking flask and suitable grindingballs or glass beads are required.

The famous MSK Cell Homoge-nizer uses glass beads to disrupt acooled sample. This is the mostefficient way to disrupt yeastcells, bacteria, fungal spores orsmall algae – materials whichare difficult to homogenize withother techniques. Shaking flasks,glass beads and cooling capillaryare all available from B. BraunBiotech International.

The Potter S is well known amongscientist who want to disruptsoft tissues or cultured cells. In addition this unit can be usedto disaggregate bacterial colonies– this has been used for testingsurface disinfectants. Cylindersand pestles for volumes betweenless than 2 ml and up to 60 mlare available in 3 different ver-sions. An integrated cooling ves-sel allows to cool the sample dur-ing homogenization. Due to itsgentle action the Potter S caneven be used to isolate nucleifrom cells.

The LABSONIC® homogenizers areapplied for disintegrating cellsusing ultrasound. Of course,emulsions can also be generated.The LABSONIC®M is a small unitwith an output of up to 100W.The operating frequency of 30 kHz reduces cavitation noises.Due to its innovative designprobes as small as 0.5 ml can becombined with this instrument.Thus even small volumes can besonicated.

Ordering information:

Cat.-No. Description

BBI-8531722 Mikro-Dismembrator U, 230 V, 50 Hz

BBI-8530220 MSK Cell Homogenizer

BBI-8533024 Homogenizer Potter S, 230 V, 50 Hz

BBI-8535027 LABSONIC M, 100 W, 230 V, 50 Hz

BBI-8535108 LABSONIC P, 400 W, 230 V, 50 Hz

For accessories please refer to the brochures!

The LABSONIC®P has a power of 400 W thus enabling to treat muchlarger sample volumes. Both instruments allow to use flow-throughchambers for working under sterile conditions. For sonicated contami-nated samples, e. g. radioactively labeled material, the LABSONIC®Pcan be equipped with a cup probe. lf desired, these LABSONlCs can becontrolled via a special PC card.

Hand homogenizers are often used in the laboratory for simple samplepreparations, e. g. crushing tissue samples. You can choose from arange of Dounce type models from 1 ml to 60 ml with 2 differentgaps. Thus you can switch from L models (wide gap) to S models (narrow gap) for finer homogenization.

Altematively, choose an Eppendorf or conical type.

100

Sartorius AG offers a comprehen-sive line of centrifuges rangingfrom small centrifuges for reac-tion vials up to floor-standingmodels with a capacity of up to6 L. Of course all our centrifugescomply with the relevant ECregulations and are CE marked.All centrifuges feature a brush-less drive for reduced mainte-nance. Running speeds may beentered as rpm or g-force values.All refrigerated units are CFC-free.

The small centrifuges have ashort spin function: the unitcounts and displays the timespent for this run. This way, itbecomes very easy to treat sever-al samples the same way

The centrifuge 2-16K and alllarger models have an automaticrotor recognition to preventoverspeeding. In addition thesecentrifuges can calculate g-forces from rpm values and viceversa. As an option, free pro-gramming allows to define andstore individual centrifuge runs.

For models 3K30, 6-15|6K15 and8K10 free programming is inte-grated as a standard.

Depending on your exact appli-cation you can choose betweenrefrigerated and non-refrigeratedversions. Of course, PCR tubes orstrips may be spun in our cen-trifuges. For special applications,such as oil analysis, even heatedcentrifuges can be delivered.Please enquire with your localrepresentative for details.

To help you select the appropriate unit, please consult the guidelinebelow. Upon request, we will provide you with brochures giving detailsabout the individual units and their accessories

Guide on selection of centrifuge models –small (1-13) to large floor-standing model (8K10)

Model Refrig Max. Max. Suitable tubes (ml)speed speed Angle Swing rotor out rotor

1-13 no 13,000 11,800 0.2 to 2.2, hematocrit

1-15 no 14,000 12,000 0.2 to 2.2, hematocrit, PCR

1-15K yes 14,000 14,000 0.2 to 2.2, hematocrit, PCR

1-6 no 5,650 n.a. 4.5, 5, 7, 15

2-5 no n.a. 3,900 0.2 to 100

2-16 no 15,000 12,000 0.2, to 100, microtiter, PCR

2-16K yes 15,300 14,000 0.2 to 100, PCR

3-16 no 14,500 5,000 0.2 to 200, microtiter, cyto

3-16K yes 15,300 5,500 0.2 to 200, microtiter, cyto

3-18K yes 18,000 5,500 0.2 to 200, microtiter, cyto

3K30 yes 30,000 10,000 0.2 to 125

4-15 no 13,500 4,500 0.2 to 500, microtiter

4K15 yes 15,000 5,100 0.2 to 500, microtiter

6-15 no 12,500 4,500 0.2 to 750, microtiter,blood bags

6K15 yes 15,000 5,100 0.2 to 750, microtiter,blood bags

8K10 yes 10,500 5,130 0.2 to 1000, microtiter,blood bags

n.a. = not applicable

For accessories and order details please consult the brochures for theindividual centrifuge models.

Laboratory centrifuges.

101


AirPort MD8.Accu-power, portable air sampler.

AirPort MD8 is the new air sam-pler for the pharmaceuticalindustry, the biotechnology, thefood and beverage industry, forhospitals environmental care andfor works safety.

AirPort MD8 offers followingbenefits:

• Accu-powered and portable foruniversal use.

• Accu-control guarantees constant performance duringthe air sampling.

• Ergonomic design and easy toclean.

• Flexible adjustment possibilitiesof the volume flow and thesample volume.

• The device can be calibratedlocally.

AirPort MD8 uses the gelatinmembrane filter method guaran-teeing reliable and exact meas-urement results.

SpecificationsAirPort MD8Volume flow regulation by inte-grated anemometer. Volume flowadjustable in three steps: 30 l/min., 40 l/min and 50 l/min.Fixed given sample volumes:25, 50, 100, 250, 500, 750 and 1000 litres.In addition, the sample volumecan be chosen in 5-liter steps.

Operational life with one store-loading:approx. 4.5 hoursNoise development:For gelatin membrane filters 48 dB (A)Weight: approx. 2.5 kgDimensions (L + W + H):300 + 135 + 165 mm

Power supply:Store:NiMH 16.8 Volt/3800 mAhBattery charger inlet:100–240 V/47–63 Hz/600 mABattery charger outlet:24 V/1000 mALoading time:approx. 4.5 hours for emptystore.

Order informationAirPort MD8, complete withholder (17801) for gelatin dispos-able units and battery charger(1ZE-0006),Order no. 16757Accessories for AirPort MD8Adapter for disposable gelatinfilter units, order no.17801Battery charger, order no. 1ZE-0006Further accessories on request!

ConsumablesDisposable gelatin units (pack of10, each unit sterile packed in apolyethylene bag),Order no.17528-080-ACD

Disposable gelatin units (pack of10, each unit three times packed,sterile, in a polyethylene bag),Order no.17528-080-BZD

Further consumables on request!

102

The system consists of the MD8airscan air sampler and dispos-able gelatin filter units. The system is routinely used for thequantitative detection of air-borne organisms, mainly in sterilespaces of classe A and B (classifi-cation according to „EU-Guidefor GMP“) or isolators. The veryhigh air flow rate of 8 m3/henables isokinetic sampleremoval at flow speed usual inlaminar flow as well as for thefiltration of 1 m3 air very quickly(less than 8 minutes). The filterunit can be placed far way fromthe air sampler.

The MD8 airscan air samplerallows to adjust selectively andeasily air flow rate and sampleremoval speed. By means of aspecially developed calibrationunit (see accessories) theuser can calibrate the MD8airscan locally, e.g. within thescope of validation steps.

After removing the sample, thegelatin filter can be placeddirectly on the agar culturemedium for incubation andcolony growth.

Specifications for gelatin filtersGelatin filter Water-soluble, pore size 3 µm. Diameter

80 mm. Thickness approx. 250 µm. Max. temperature 60°C.Residual dampness content 46%–49%. Air flow rates approx. 2.7/min./cm2 at ∆p = 0.05 bar. Retention rates: a) Bac. subtilis niger 99.9995%

at 0.25 m/s inlet velocityb) Coli-Phages: phage T1, 99.9% at 0.3 m/s inlet velocity and 50% rel. airhumidity. Phage T3, 99.94% at 0.3 m/s inlet velocityand 80% rel. air humidity


Limiting conditions Room temperature, max. 30°C, of gelatin filter units rel. air humidity 85%Dimensions Diameter 93 mm, Height 16 mmSterilization supplied presterilized by gamma irradiation

Specifications for the MD8 airscan air samplerAir flow rate 2.0 m3/h – 8 m3/h adjustable in

100 litre stepsTime switch off 1–99 minutes, adjustable in 1 minute stepsMax. divergence ±5% in a temperature range of 15°–35°CNoise development for gelatin membrane filters, max. 62 dB (A)Weight ca. 6.5 kgDimensions 375 + 242 + 228 mm (L + W + H)Correction of the If the used air flow rate can not be air flow-adjustments achieved, the display shows the max.

achieved flow rate for a correspondingnew adjustment below this value

Order Numbersa) for the MD8 airscan air sampler16746 MD8 airscan air sampler, 230 V, 50 Hz 50/60 HZ16747 MD8 airscan air sampler, 115 V, 60 Hz 50/60 HZ16748 MD8 airscan air sampler, 100 V, 50–60 Hz 50/60 HZEach version can be switched from 50 to 60 Hz and back.17801 Holder for disposable gelatin filter units

b) for disposable gelatin filter unitsSterile, pack of 10:17528-080 ACD Individually packed in1 Polyethylene

bag each17258-080 BZD Individually packed in 3 Polyethylene

bags each

The detection of microorganisms and viruses out of air. Quantitatively and reproducible with the MD8 airscan.

103

Air sampler

Accessories for the MD8 air sampler.

a) Calibration unit Order number:16740

The user himself can calibrate theMD 8 airscan and AirPort MD8directly on the job by means ofthe calibration unit*. This is absolutely necessary above all within the scope of validation steps, for which it isimportant, that the shown airflow rate (desired value at theMD8) corresponds to the actualair amount (actual value at thecalibration device). On request, a calibration certificate of theGerman Calibration Service canbe available. The calibration unitis supplied complete with batterycharger| mains appliance (country-related), filter holder,connectors set and connectiontube (PVC, 2m).

Specifications:Dimensions,a) Lenght without filter holder,

295 mmb) Width, 174 mmc) Height with handling, 246 mm

Connectors, quick locks (bayonetprinciple)Operational life with full accu,ca. 70 hoursWeight, ca. 5.6 kgCharge time for empty accu,ca. 30 hoursMax. fault, to 3.2 m 3/h, ±3%Max. fault, 3.2 to 16 m 3/h, ±1.5%Measuring range, 1.6–16 m 3/hType of protection, IP 40

Ambient conditions:a) Rel. air humidity, 30%–80%b) Temperature,min. –10°C, max.

40°C

* Alternatively, a maintenance agreementcan be signed. Within the scope of thecontractual services, Sartorius technicianswill carry out a calibration of the MD8 atregular intervals.

b) Tubing and connectors set

Order numbers:17085 Plastic hose, 2 m17088 Plastic hose, 5 m17662 Silicone hose, 1 m

(25 + 3 mm)17657 Connectors set

If the disposable gelatin filterunit is not placed directly at theMD8, but in a distance from it, a flexible plastic hose (2m or 5m),a connectors set and, if not avail-able, a holder (tripod 16970, dou-ble socket 16976, clamp 17037)are necessary for the connectionbetween filter and device.

The autoclavable silicone hose isused instead of the flexible plastic hose, if the MD 8 airscanhas to be used in sterile rooms,operating rooms, isolators, etc.

With this hose attached to the airopenings, the waste air can be ledoff into an other room.

c) Case Order number: 17208Stable case for the transport andthe storage of a MD 8 airscanincl. accessories.

Gelatin membrane filter

Gelatin membrane filters are stillavailable as 80 mm filter discs,suitable for the filter holder sup-plied with the MD8 airscan.

Order numbers:12602-080 ALKGelatin membrane filter, 80 mm, sterile (pack of 50)

The filters are sterile supplied, butthe filter holders have to be ster-ilized by dry heat (180°C, 2 h) andthen equipped with the filtersunder sterile conditions. For performing routine check-ups, astack is recommended in thiscase.

Order numbers:17656 Aluminium stack

It consists of a middle part, 10numbered filter holders and 2end caps. The stack is first steril-ized (by 180°C dry heat,2 h), andthen equipped with the filtersunder sterile conditions (LF-workbench). The prepared filterholders are put on a side of themiddle part. After removing thesample, the inserted filter holdersare put on the other side of themiddle part, so that used andnot-used filter holders are sepa-rated from each other.

Replacement parts for the stack:17655 Individual filter holders17660 Middle part17661 End cap

104

This automatically working,micro-processor controledintegrity tester is used for deter-mining the bubble point of discfilter systems, as well as for diffusion and bubble point testsof capsules, mini and standardfilter cartridges.

It features several benefits: Continuously displays updates ofthe data measured and docu-mentation of the data measuredvia printer. Battery-operable (onecharge of the batteries is suffi-cient to run at least 20 tests).Special programme for measur-ing and recording the net inletvolume. 3 user programmes canbe stored. Can be calibrated. RS 232 interface.

Specifications for Sartocheck Junior BP plusDimensions (W + D + H) 260 + 330 + 85 mmLead-in voltage 220/240V 50Hz, 110/120V 60Hz(for the battery charger) Weight 4.8 kgMax. inlet pressure 8,000 mbarMax. transmission distance 15 m

Measuring rangeBubble point 0.5–6 barDiffusion 0 –999 ml/minPressure drop rate 0–200 mbar/minNet volume of the system 0.05–5 lTest pressure 100–6,000 mbar

Measuring accuracyBubble point ±0.1 barDiffusion determination ±6%Pressure regulation ±0.4% at 2.5 barVolume determination ±5%

Type of protection against splashes of water: IP 54Test record, on the left, a present example. In order to increase the test transparency and to ensure the detectability of the results, net inlet volumes, test pressure (actual value), pressure drop value andtest time are also documented.

Order number for Sartocheck Junior BP plus16296 Sartocheck Junior BP plus

Equipment supplied: Sartocheck Junior BP plus integrity tester. Battery charger.Pressure inlet tubing with pressure filter. Pressure outlet tubing. 1 litrereference tank. Colour ribbon cassette. Paper roll. Operating instruc-tions. Test certificate. Calibration certificate.

Sartocheck® Junior BP plus.The battery-operable diffusion and bubble point tester.

105

Integrity testers

The new standard in integrity test measuring: Sartocheck®3.

With the development of the firsttester for automatical testing ofthe integrity of membrane filtersworldwide, Sartorius came upwith an important pioneeringfeat in 1981. This increased significantly theprocess safety, particularly insterile filtration. As the Sartocheck II before, the Sartocheck® 3 sets again com-pletely new standards concerningobjective valuaton, easy handling, use under productionconditions - key word protectionagainst splashes of water, highreliability, exact measuring tech-niques, fast results and possibili-ties of integration in modern,automated process control.

Sartocheck 3 also meets all standards required for electronicmeasuring instruments for thevalidation of pharmaceuticalprocesses. An extensive validationset is available.

Specifications for Sartocheck 3Dimensions 460 + 360 + 65 +126 mm (W + D + H1+ H2) Lead-in voltage 85–240 V; 47–440 HzOperating pressure 3,000–9,999 mbarPressure sensor absolut. class 0.2Weight 11.2 kgOperational field, integrity test of disc filters (bubble point), capsules,mini cartridges and cartridge systems up to 50 litre net inlet volume.

Measuring range and accuracyTest pressure 100-6,000 mbar, 0.2 % of the max. valuePressure drop 0-999 mbar, ±1 mbarSystem net volume 50,000 ml, ±4%Programming code-word protectedProgramme number memory card system allows saving of 240

test programmesType of protection against splashes of water: IP 54Test documentation, on the left, a Sartocheck 3 test record.In the first part of the “test results“, a pressure drop diagram showsthe pressure curve during the test phase.In the second part, the really measured parameters and the test resultsare listed.The test is then evaluated by comparing the entered limit data.If the test is not passed, the concerned measurements and the evalua-tion are printed out in red.

Test methods,Bubble point test; Diffusion test; Multidiffusion test.Water intrusions test (Sartorius newly developed the WIT for “in place“testing of air filter cartridges and hydrophobic membrane filters)

Order Numbers for Sartocheck 316286 Sartocheck 3.Equipment supplied Sartocheck 3 with memory card. Pressure

inlet tubing (ø 6 mm) with air pressure fil-ter. Pressure outlet tubing (ø 4 mm).Colour ribbon cassette (black/red). Printerpaper. Test certificate. Calibration certificate. Outline instruc-tions. Operating instructions. Power con-necting lead (country-related).

106

Cellulose Cellulose Reg. PTFE Poly- Glass Polycar- Poly- Sartobran P Sartofluoracetate nitrate Cellulose amide fibre bonate ether- cartridge cartridge

sufone Solvents 111 113 184 118 250 134 230 154

Acetone – – ■ ■ – ■ ❏ – – E

Acetonitrile ? ? ■ ■ – ? ? ■ ? ?

Gasoline ■ ■ ■ ■ ■ ■ ■ ■ V –

Benzene ■ ■ ■ ■ ■ ■ ? ■ – –

Benzyl alcohol ❏ ❏ ■ ■ ■ ■ ? – ❏ ■

n-Butyl acetate ❏ – ■ ■ ■ ■ ■ ■ E ?

n-Butanol ■ ■ ■ ■ ■ ■ ■ ■ ■ ■

Cellosolve ■ – ■ ■ ? ■ – ■ – –

Chloroform – ■ ■ ■ ■ ■ – – – –

Cyclohexane ❏ ❏ ■ ■ ? ■ ■ – ❏ V

Cyclohexanone – – ■ ■ ■ ■ ? ? – –

Diethylacetamide – – ■ ■ ■ ■ ? ? – ?

Diethyl ether ■ – ■ ■ ■ ■ ■ ? – –

Dimethyl formamide – – ❏ ■ ❏ ■ – ? – ■

Dimethylsulfoxide – – ■ ■ ■ ■ – – – ■

Dioxane – – ■ ■ ■ ■ – ■ – ■

Ethanol, 98% ■ ❏ ■ ■ ■ ■ ■ ■ ■ ■

Ethyl acetate – – ■ ■ ■ ■ ? – – –

Ethylene glycol ■ ❏ ■ ■ ? ■ ■ ■ ■ ■

Formamide ? ? ? ■ ? ■ – ? – ■

Glycerin ■ ■ ■ ■ ■ ■ ■ ■ ■ ■

n-Heptane ■ ■ ■ ■ ? ■ ? ? ■ V

n-Hexane ■ ■ ■ ■ ■ ■ ■ ? V –

Isobutanol ❏ ❏ ■ ■ ■ ■ ■ ? – ■

Isopropanol ■ ❏ ■ ■ ■ ■ ■ ■ ■ ■

Isopropyl acetate ❏ – ■ ■ ? ■ ? ■ – ■

Methanol, 98% ■ – ■ ■ ? ■ ■ ■ ■ ■

Methyl acetate – – ■ ■ ■ ■ ? – – ■

Methylene chloride – ❏ ■ ■ ■ ■ – – – –

Methyl ethyl ketone – – ■ ■ ■ ■ ? – – ■

Methyl isobutyl ketone ■ – ■ ■ ■ ■ ? ? – –

Monochlorobenzene ■ ■ ■ ■ ■ ■ – ? V V

Nitrobenzene ■ ❏ ■ ■ ■ ■ – ? – –

n-Pentane ■ ■ ■ ■ ■ ■ ■ ? V V

Perchlorethylene ■ ■ ■ ■ ■ ■ ■ ? V V

Pyridine – – ■ ■ ■ ■ – – – –

Carbon tetrachloride ❏ ■ ■ ■ ■ ■ ? ■ – ?

Tetrahydrofuran – – ■ ■ ■ ■ – – – –

Toluene ■ ■ ■ ■ ■ ■ ? ■ – –

Chemical compatibilitya) Filter materials and Mini Cartridges.

107

Chemical compatibility

108

Cellulose Cellulose Reg. PTFE Poly- Glass Polycar- Poly- Sartobran P Sartofluoracetate nitrate Cellulose amide fibre bonate ether- cartridge cartridge

sufone Solvents 111 113 184 118 250 134 230 154

Trichlorethane ❏ ■ ■ ■ ? ■ ? ? – ?

Trichlorethylene ■ ■ ■ ■ ■ ■ – ■ – ?

Xylene ■ ■ ■ ■ ■ ■ ■ ■ – –

Acids

Acetic acid, 25% ■ ■ ■ ■ ❏ ? ❏ ■ ■ ?

Acetic acid, 96% – – ■ ■ – ? ? ■ – ■

Hydrofluoric acid, 25% ■ ❏ ❏ ■ – ? ■ ? – –

Hydrofluoric acid, 50% ■ ❏ – ■ – ? ■ ? – –

Perchloric acid, 25% – ❏ ❏ ■ – ? ? ? – ■

Phosphoric acid, 25% ■ ❏ ❏ ■ – ? ? ? ■ ■

Phosphoric acid, 85% ❏ ❏ ❏ ■ – ? – ? – V/E

Nitric acid, 25% – ❏ – ■ – ? ■ ■ – V

Nitric acid, 65% – – – ■ – ? ■ ■ – –

Hydrochloric acid, 25% – ❏ – ■ – ? ■ ■ – V/E

Hydrochloric acid, 37% – – – ■ – ? ■ ■ – V/E

Sulfuric acid, 25% – ❏ ❏ ■ – ■ ? ■ – ■

Sulfuric acid, 98% – – – ■ – ? – ? – –

Trichloroacetic acid, 25% – ❏ ■ ■ – ? ? ? – ■

Bases

Ammonium, 1N ■ ■ ❏ ■ ■ ■ – ■ E ■

Ammoniumhydroxide,25% – ❏ – ❏ ■ ❏ – ■ – ■

Potassium hydroxide,32% – – ❏ ■ ❏ ❏ – ■ – ■

Sodium hydroxide, 32% – – ❏ ■ ❏ ❏ – ■ – ■

Sodium, 1N ❏ – ❏ ■ ■ ■ – ■ – ■

Aqueous solutions

Formalin, 30% ❏ ■ ❏ ■ ❏ ■ ■ ■ – ■

Sodium hypochlorite, 5% ■ ❏ ■ ■ ❏ ■ ? ? – ■

Hydrogen peroxide, 35% ■ ■ ❏ ■ ❏ ? ? ? ■ ■

Key to symbols

■ = compatible ❏ = limited compatibility– = not compatible ? = not tested

E = compatible after replacing silicone O-ring with an EPDM O-ringV = compatible after replacing the silicone O-ring with a Viton O-ring

Contact time: 24 hours at 20°CChemical compatibilities can be influenced by various factors.Therefore, we recommend that you confirm compatibility with theliquid you wish to filter by performing a trial filtration run beforeyou begin with actual filtration.

a) Filter materials and Mini Cartridges.

b) Filter holder, Cartridge housing and O-ring Materials.

109

Chemical compatibilityGlass Poly- Poly- PTFE Stainless EPDM PTFE Silicone Vitroncarbonate propylene steel O-ring O-ring O-ring O-ring

Solvents

Acetone ■ ❏ ■ ■ ■ ■ ■ – –

Acetonitrile ■ ? ■ ■ ■ ❏ ■ – ■

Gasoline ■ ❏ ■ ■ ■ – ■ – ■

Benzene ■ – – ■ ■ – ■ – ■

Benzyl alcohol ■ – ■ ■ ■ ❏ ■ ■ ■

n-Butyl acetate ■ – ❏ ■ ■ ■ ■ – –

n-Butanol ■ ■ ■ ■ ■ ■ ■ ■ ■

Cellosolve ■ – – ■ ■ ❏ ■ – –

Chloroform ■ – – ■ ■ – ■ – ■

Cyclohexane ■ ❏ ■ ■ ■ – ■ – ■

Cyclohexanone ■ – ■ ■ ■ – ■ – –

Diethylacetamide ■ – ? ■ ■ ? ■ ■ –

Diethyl ether ■ – ❏ ■ ■ – ■ – –

Dimethyl formamide ■ – ■ ■ ■ ■ ■ ❏ –

Dimethylsulfoxide ■ ? ? ■ ■ ? ■ ❏ –

Dioxane ■ – ❏ ■ ■ ■ ■ – –

Ethanol, 98% ■ ■ ■ ■ ■ ■ ■ ■ ■

Ethyl acetate ■ – ■ ■ ■ ■ ■ – –

Ethylene glycol ■ ■ ■ ■ ■ ■ ■ ■ ■

Formamide ■ – ■ ■ ■ ■ ■ – ❏

Glycerin ■ ❏ ■ ■ ■ ■ ■ ■ ■

n-Heptane ■ ■ ■ ■ ■ – ■ ■ ■

n-Hexane ■ ■ ■ ■ ■ – ■ – ■

Isobutanol ■ ■ ■ ■ ■ ■ ■ ■ ■

Isopropanol ■ ❏ ■ ■ ■ ■ ■ ■ ■

Isopropyl acetate ■ ■ ■ ■ ■ ■ ■ – –

Methanol, 98% ■ – ■ ■ ■ ■ ■ ■ ■

Methyl acetate ■ ? ■ ■ ■ ■ ■ – –

Methylene chloride ■ – – ■ ■ – ■ – ❏

Methyl ethyl ketone ■ – ■ ■ ■ ■ ■ – –

Methyl isobutyl ketone ■ – ? ■ ■ – ■ – –

Monochlorobenzene ■ – ■ ■ ■ – ■ – ■

Nitrobenzene ■ – ❏ ■ ■ – ■ – –

n-Pentane ■ ■ ■ ■ ■ – ■ – ■

Perchlorethylene ■ – ❏ ■ ■ – ■ – ■

Pyridine ■ – ❏ ■ ■ – ■ – –

Carbon tetrachloride ■ – ❏ ■ ■ – ■ – ■

Tetrahydrofuran ■ – ❏ ■ ■ – ■ – –

Toluene ■ – ■ ■ ■ – ■ – ❏

b) Filter holder, Cartridge housing and O-ring Materials.

110

Key to symbols



Glass Poly- Poly- PTFE Stainless EPDM PTFE Silicone Vitroncarbonate propylene steel O-ring O-ring O-ring O-ring

Solvents

Trichlorethane ■ – ? ■ ■ – ■ – ■

Trichlorethylene ■ – – ■ ■ – ■ – ■

Xylene ■ – ❏ ■ ■ – ■ – ❏

Acids

Acetic acid, 25% ■ ■ ■ ■ ■ ■ ■ ■ –

Acetic acid, 96% ■ – ■ ■ ■ ■ ■ ? –

Hydrofluoric acid, 25% – – ■ ■ – ❏ ■ – ❏

Hydrofluoric acid, 50% – – ■ ■ – ❏ ■ – ❏

Perchloric acid, 25% ■ ❏ ■ ■ – ■ ■ – ■

Phosphoric acid, 25% ■ ❏ ■ ■ ❏ ■ ■ – ■

Phosphoric acid, 85% ■ ❏ ■ ■ ❏ ■ ■ – ■

Nitric acid, 25% ■ – ■ ■ – ❏ ■ – ■

Nitric acid, 65% ■ – – ■ – – ■ – ■

Hydrochloric acid, 25% ■ ❏ ■ ■ – ❏ ■ – ■

Hydrochloric acid, 37% ■ – ■ ■ – ■ ■ – ■

Sulfuric acid, 25% ■ ■ ■ ■ ❏ ■ ■ – ■

Sulfuric acid, 98% ■ – – ■ – – ■ – ■

Trichloroacetic acid, 25% ■ ❏ ■ ■ – ■ ■ – –

BasesAmmonium, 1N ■ – ■ ■ ■ ■ ■ – –

Ammonium hydroxide, 25% ■ – ■ ■ ■ ■ ■ ■ –

Potassium hydroxide, 32% ■ – ■ ■ ■ ■ ■ ❏ ❏

Sodium hydroxide, 32% ■ – ■ ■ ■ ■ ■ ❏ ■

Sodium, 1N ■ – ■ ■ ■ ■ ■ ■ ■

Aqueous solutionsFormalin, 30% ■ ■ ■ ■ ■ ■ ■ ❏ ■

Sodium hypochlorite, 5% ■ ■ ■ ■ ■ ■ ■ ■ ■

Hydrogen peroxide, 35% ■ ■ ■ ■ ■ ■ ■ ■ ■

c) Ready-to-connect Filtration Units.

111

Chemical compatibilityMidisart Minisart Minisart Minisart Minisart Sartobran Sartobran P Sartofluor Sartolab2000 HY RC SRP 300 Capsule Capsule P20

Solvents

Acetone ■ – – ■ – – – ■ –

Acetonitrile ■ – ? ■ ■ ? ? ? ?

Gasoline ■ ■ ■ ■ ■ ■ ■ ■ ❏

Benzene ■ – – ? ■ – – ❏ –

Benzyl alcohol ■ ? ? ? ■ ❏ ❏ ■ –

n-Butyl acetate ■ – – ? ■ ■ ■ ■ –

n-Butanol ■ ❏ ❏ ■ ■ ■ ■ ■ ■

Cellosolve ❏ – – ■ ❏ – – ❏ –

Chloroform ■ – – ■ ■ – – ■ –

Cyclohexane ■ – – ? ■ ❏ ❏ ■ ❏

Cyclohexanone ■ – – ? ■ – – ■ –

Diethylacetamide ■ – – ■ ■ – – ■ –

Diethyl ether ■ ? ? ? ■ ❏ ❏ ■ –

Dimethyl formamide ■ – – ? ■ – – ■ –

Dimethylsulfoxide ■ – – ■ ■ – – ■ –

Dioxane ■ – – ■ ■ – – ❏ –

Ethanol, 98% ■ – – ■ ■ ■ ■ ■ ■

Ethyl acetate ■ ❏ ❏ ■ ■ – – ❏ –

Ethylene glycol ■ ? ? ■ ■ ■ ■ ■ ■

Formamide ■ ? ? ? ■ ? ? ■ –

Glycerin ■ ■ ■ ? ■ ■ ■ ■ ❏

n-Heptane ■ ■ ■ ? ■ ■ ■ ■ ■

n-Hexane ■ ■ ■ ■ ■ ■ ■ ■ ■

Isobutanol ■ ❏ ❏ ■ ■ ❏ ❏ ■ ❏

Isopropanol ■ ❏ ❏ – ■ ■ ■ ■ ❏

Isopropyl acetate ■ ❏ ❏ ? ■ ❏ ❏ ■ ❏

Methanol, 98% ■ – – ■ ■ ■ ■ ■ –

Methyl acetate ■ – – ? ■ – – ■ –

Methylene chloride ■ – – ■ ■ – – ❏ –

Methyl ethyl ketone ■ – – ■ ■ – – ■ –

Methyl isobutyl ketone ■ ? ? ? ■ ? ? ■ –

Monochlorobenzene ■ ? ? ? ■ ■ ■ ■ –

Nitrobenzene ■ ? ? ? ■ ❏ ❏ ■ –

n-Pentane ■ ■ ■ ■ ■ ■ ■ ■ ■

Perchlorethylene ■ ❏ ❏ ? ■ ❏ ❏ ■ –

Pyridine ■ – – ? ■ – – ■ –

Carbon tetrachloride ■ ❏ ❏ ? ■ ❏ ❏ ■ –

Tetrahydrofuran ■ – – ■ ■ – – ❏ –

Toluene ■ – – ■ ■ ■ ■ ■ –

c) Ready-to-connect Filtration Units.

112

Key to symbols



Midisart Minisart Minisart Minisart Minisart Sarto- Sartobran P Sartofluor Sartolab2000 HY RC SRP bran 300 Capsule Capsule P20

Solvents

Trichlorethane ■ ❏ ❏ ■ ■ ? ? ■ –

Trichlorethylene ❏ ? ? ? ❏ – – – –

Xylene ■ – – ■ ■ ❏ ❏ ■ –

Acids

Acetic acid, 25% ■ ❏ ❏ ? ? ■ ■ ■ ■

Acetic acid, 96% ■ – – ? ■ – – ■ –

Hydrofluoric acid, 25% ■ ❏ ❏ ? ■ ■ ■ ■ –

Hydrofluoric acid, 50% ■ ❏ ❏ ? ■ – – ■ –

Perchloric acid, 25% ■ ? ? ? ■ – – ■ –

Phosphoric acid, 25% ■ ■ ■ ? ■ ■ ■ ■ ■

Phosphoric acid, 85% – ? ? ? – ❏ ❏ – ❏

Nitric acid, 25% ■ – – ? ■ – – ■ –

Nitric acid, 65% ■ – – ? ■ – – ❏ –

Hydrochloric acid, 25% ■ – – ? ■ – – ■ –

Hydrochloric acid, 37% ■ – – ? ■ – – ■ –

Sulfuric acid, 25% ■ – – ? ■ – – ■ –

Sulfuric acid, 98% ■ – – ? ■ – – ■ –

Trichloroacetic acid, 25% ■ – – ■ ■ – – ■ –

Bases

Ammonium, 1N ■ ■ ■ ? ■ ■ ■ ■ –

Ammonium hydroxide, 25% ■ ❏ ❏ ? ■ ❏ ❏ ■ –

Potassium hydroxide, 32% ■ – – ? ■ – – ■ –

Sodium hydroxide, 32% ■ – – ? ■ – – ■ –

Sodium, 1N ■ ❏ ❏ ? ■ ❏ ❏ ■ –

Aqueous solutions

Formalin, 30% ■ – – ? ■ ❏ ❏ ■ ❏

Sodium hypochlorite, 5% ■ ■ ■ ? ■ – – ■ ■

Hydrogen peroxide, 35% ■ ■ ■ ? ■ ■ ■ ■ ■

2

time(min)

A 28

0

13

100% B

1 2 3 4

Conditions:Flow rate: 50 ml/minBuffer A: 20 mM Tis pH 8,5Buffer B: A + 0.4 M KCLGradient: 0–100% B

Separation of 3 standard proteinson Q15 (1 = Transferrin, 2 = Oval-bumin, 3 = b-Lactoglobulin-A)

Different application notes aboutthe purification and concentra-tion of proteins and monoclonalantibodies, about the seperationof nucleotides and plasmids, andabout the removal of endotoxinsand DNA are available.

The membrane ion exchangers in syringe filter format are theultimate for simple, ultra-rapidconcentration of proteins fromhighly dilute solutions or proteinseparation from a mixture. Four different types of membraneion exchangers, S, Q, C and D areavailable, with the followingfunctional groups:

Functional groups Sulfonic acid (type S)R-CH2 -SO3

––

Strongly acidic cation exchanger

Quaternary ammonium (type Q)R-CH2-N+-(CH3)3Strongly basic anion exchanger

Carboxyl groups (type C)R-COOWeakly acidic cation exchanger

Diethylamine (type D)R-CH2-N+-(C2H5)2Weakly basic anion exchanger

They are offered as ready-to-usedisposable units with 5 cm2

adsorption area (MA5 units) andas reusable units with 15 cm2 and100 cm2 adsorption area (MA15and MA100 units).

The units are easy to handle andcompatible with simple syringes,peristaltic pumps or existingworkstations, so that no time islost in changing from a traditional chromatography col-umn to the new membrane ionexchangers. Procedures whichtake one hour or more can nowbe reduced to minutes.

Membrane Adsorbers can be usedwith an FPLC® system. The con-nection is very easy because ofthe Luer lock inlet/outlet connec-tors of the MA units. Worksta-tions with high performance flowrates, e.g. the BioPilot system,can take full advantage of thereproducible separations at highflow rates which are possiblewith MA units.

The figure shows the separationprofiles of reference proteins at3 different flow rates on a BioPi-lot system. No sacrifice in separa-tion performance or capacity wasfound under the applied condi-tions, even when the cycle time isreduced by a factor of 10.

The chemical stability (pH 2–13)of Membrane Adsorbers allowsregeneration by using aggressivecleaning agents. It is possible toregenerate the MA units with 1NNaOH for one hour. As Fig. 7shows, 100 cycles of protein sep-aration using an FPLC® systemwere done without any change inresolution (one cycle includes:equilibration, loading, washing,elution with KCl andregeneration with 0.2 N NaOH).

SartobindTM Membrane AdsorbersA separation technology based on microporous membrane ion exchangers.

113

Membrane Adsorbers

Specifications for MA5, MA15 and MA100 units.

MA5 disposable units MA15 reusable units MA100 reusable units

Membrane material crosslinked crosslinked crosslinkedregenerated cellulose regenerated cellulose regenerated cellulose

Adsorption area* 5 cm2 15 cm2 100 cm2

Housing material MBS-copolymer polysulfone polysulfone

Inlet connector female Luer lock female Luer lock female Luer lock

Outlet connector male Luer lock male Luer lock male Luer lock

Protein binding 4 mg | S5 unit 12 mg | S15 unit 80 mg | S100 unitcapacity** 4 mg | Q5 unit 12 mg | Q15 unit 80 mg | Q100 unit

3 mg | C5 unit 9 mg | C15 unit 60 mg | C100 unit3 mg | D5 unit 9 mg | D15 unit 60 mg | D100 unit

Flow rate at 1 bar > 100 ml/min > 50 ml/min > 75 ml/min

Hold-up volume 0.8 ml 1.0 ml 4.2 ml

Maximum pressure 6 bar 7 bar 6 bar

pH stability – pH 2–13 pH 2–13

Storage before use dry at room dry at room dry at room temperature temperature temperature

Regeneration not applicable 1 hr treatment with 1 N NaOH or 1 N HCl at room temperature. Back flushing is possible.

Storage after use not applicable wet in buffer or saline solution at 4–8°C in thepresence of anti-microbial agents. Do not expose to pure water or to organic solvents.

All MA types passed the USP 24 cytotoxity test with MRC-5 and USP 24 Plastics Class VI test.36.4 cm2 adsorption area ≅ 1 ml membrane volume** Reference proteins: Lysozyme for cation and BSA for anion exchangers using appropriate buffer system.

The binding capacity depends upon the MA type and conditions used.

Order numbers for MA5, MA15 and MA100 units

MA5 disposable units MA15 reusable units* MA100 reusable unitsType of exchanger (packs of 15) (packs of 2) (packs of 1)

Strong cation exchanger S5F S15X S100X

Strong anion exchanger Q5F Q15X Q100X

Weak cation exchanger C5F C15X C100X

Weak anion exchanger D5F D15X D100X

MA15 and MA45 packages include 1 Minisart 0.2 µm syringe filter for prefiltration.

114

115

ArbeitstechnikenTraining Seminar for thePharmaceutical Industry

# 862017 Microbiological Quality Controlof Sterile Products in CleanRooms and Isolators

Theoretical Aspects:• Basic principles and regulato-

ry requirements for workingunder clean room conditions

• Monitoring of microbiologicalair quality in clean rooms andisolators

• Basic principles of sterilitytesting according to EP

• Microbiological control ofambient conditions andquality management in drugmanufacturing

Practical Exercises:• Sampling of airborne micro-

organisms in laminar flowsystems and in a demo isolator

• Sterility test handling

Total course time: 2 days

Target Group:This training seminar is particu-larly suited for staff working inquality assurance and/or qualitycontrol in the pharmaceuticalindustry.

Prerequisites:The participants must be familiarwith the basic principles ofmicrobiology.

# 862008 The Use of Crossflow Filtrationin Pharmaceutics, Pharma-cology and Biotechnology

The participants will acquirestate-of-the-art knowledgeabout GMP-compliant processingusing crossflow filtration.

Theoretical Section:• Crossflow filtration theory• Membrane characterization |

membrane selection• Factors critical to perform-

ance• Scaling up• Operating conditions• Cleaning-in-place (CIP)• Steaming-in-place (SIP)• Integrity testing• Applications in biotechnology

Practical Section:• Operational set-up of the

systems• Determining the flux rate

for water• Demonstration of steaming in

place (optional)• Cell (particle) retention by

Microfiltration (model solu-tion)

• Concentration of proteinsolutions by ultrafiltration

• Removal of low-molecularweight constituents by diafiltration

• Cleaning• Discussion of the results


Target Group: Staff working in areas rangingfrom research to production. Themodular structure of the courseallows the participants to requesttheir own topics as well.

# 862024 Sterilization and IntegrityTesting of Membrane Filters

The participants in this trainingseminar on steaming-in-placeand in-place integrity testing willacquire theoretical knowledgeand practical experience in thehandling of filters used in sterilefiltration.

Theoretical Section:• Basic principles of filtration

(depth filters, membranefilters)

• Integrity testing of membranefilters (methods, testingequipment)

• Physical and theoreticalprinciples of the steamsterilization of filter lines

Practical Section:• Performing integrity tests• Practical tests with in-line

steam sterilization of filtercartridge lines


Target Group:Staff working in the areas ofproduction, quality assuranceand quality control

In-house Training at Your Company!

# 862026 Plant Hygiene in Pharmaceutical Industry

This seminar is specificallydesigned to be held in-house sothat as many employees aspossible can attend.

Contents:• Basic principles of microbio-

logy• General rules of hygiene• Critical factors• Personnel hygiene• Behavior in clean rooms• Microbiological limit values• Minimizing particle load and

germ counts• Hygiene monitoring

Total course time: 1 day

Target Group:Staff working in production and staff members involved withmicrobiological monitoring

# 862024 Sterilization and IntegrityTesting of Membrane Filters

The participants in this trainingseminar on steaming-in-placeand in-place integrity testing willacquire theoretical knowledgeand practical experience in thehandling of filters used in sterilefiltration.

Theoretical Section:• Basic principles of filtration

(depth filters, membranefilters)

• Integrity testing of membranefilters (methods, testingequipment)

• Physical and theoreticalprinciples of the steam sterili-zation of filter lines

Practical Section:• Performing integrity tests• Practical tests with in-line

steam sterilization of filtercartridge lines


Target Group:Staff working in the area ofproduction, quality assuranceand quality control

Training Seminar for the Food & Beverage andPharmaceutical Industry

# 862001 MicrobiologicalBasic Principles of ProductSafety and Plant Hygiene

Theoretical Aspects:

• Introduction to the generalmicrobiology and die mem-brane filtration technology

• Determination of germ countsin water (European DrinkingWater Regulation) and aqueous solutions

• Culture conditions• Personnel hygiene

Practical Exercises:• Introduction to microbiologi-

cal work with products withlow microbe counts

• Sample filtration runs withvarious media: water, parti-culate media, oil-containingmedia


Target Group:This training seminar is intendedfor staff members working in theareas of quality assurance and/orquality control in the pharma-ceutical industry and food andbeverage industry.

Expand your mind.Trainings and Seminars.

115

Expand your mind

116

Training Seminar for thePharmaceutical Industry

# 862023 Biotechnology Workshop Cultivation of high-cell densityEscherichia coli and InitialWork-upIn Part 1 (# 862021) of this work-shop, the participants will bedivided into small groups andwork with high cell density culti-vation in laboratory bioreactors.The pertinent subjects will bedealt with in theory and practiceIn Part 2 (#862022) the variousmethods for primary work-upwill be dealt with in theory andpractice. You can sign up forindividual sections or for alltogether under course number#862023.

Contents: The main focus of the first partwill be on the efficient high celldensity cultivation or HCDC, safeuse and operation of bioreactorsin the laboratory and how toavoid problems with sterility. Thesecond part offers an intensiveintroduction to primary work-upmethods. Topics of discussion will include ways of achievingadequate oxygen supply, geneticfundamentals of Escherichia coli,suitable vectors and the initialsteps in obtaining a target pro-tein, called product capturing.

• Laboratory bioreactors,handling methods startingwith processing up to cellharvesting

• Sterile techniques, steriliza-tion kinetics Media design forhigh cell density cultivation

• Cultivation strategies andanalysis

• Escherichia coli and vectors• Primary work-up: Centrifuga-

tion, Crossflow Filtration(micro- and ultrafiltration)

• Cell digestion• Qualification and validation

Total course time:# 802021 Part 1 High cell densitycultivation: 3 days# 802022 Part 2 Primary work-up: 2 days# 802023 Part 1 and Part 2: 5 days

Target Group:This course specifically targetsbiologists, biochemists, chemists,biotechnologists, engineers andtechnical staff who have a basicknowledge of microbiology, cul-tivation of microorganisms andmolecular biology and work onpractical applications with labo-ratory bioreactors or in the pro-cessing of fermentation productsor want to work in these areas.The most important fundamen-tals will be taught to decision-makers and laboratory managersin the fields of biotechnology,pharmaceutical biotechnologyand enzyme production.

# 862U25 Implementation of PDA TR 26The main focus of this course ison the practical implementationof the PDA Technical ReportNo. 26 according to which anyvalidation will stand up to everyaudit.

Contents:• The introduction will start by

explaining the basic differ-ences between depth filtersand membrane filters andabout the materials availablefor the production of therespective filters.

• The different integrity testswill be presented and theircorrelation to the destructivebacteria challenge test will beexplained.

• A brief historical review of thedevelopment of the GMP ideawith regard to handling sterilefilter elements will facilitateaccess to a more detailedinterpretation of the PDA TR 26.

• Using the example of theCONFIDENCE ValidationService, explanations will begiven about which tests andanalyses must be conductedand how they should be con-ducted in order to implementCGMP theory successfully.

• A concluding discussion givesthe participants the oppor-tunity to ask questions abouttheir daily work and identifyways of approaching thesolutions.

Total course time: 1 day

Target Group:Staff working in the field ofquality assurance | quality con-trol and production

Training Seminar for the Foodand Beverage Industry

# 862019 Sensory Analysis ofNon-alcoholic BeveragesThis basic and advanced trainingcourse on sensory science willteach you about current topics inthe theory and practice of sensoranalysis.

Theoretical Section:• The importance of sensory

analysis as a method and toolfor ensuring consistentproduct quality

• Current topics in the theoryand practice of sensoryanalysis

• How do I carry out propertests in my everyday routine?

• Basic theory

• Methods of sensor analysisaccording to DIN and ISOstandards

• Drafting a profilogram

Practical exercises:• Basic test (taste thresholds)• Taste testing (Paired compari-

son, Triangular test, Duo-triotest)

• Olfactory tests• The practical part will be con-

cluded with a test of yourpersonal performance in atasting and smelling


Target Group:Staff working in the fields ofquality assurance | qualitycontrol and test panel membersin the non-alcoholic beverageindustry

# 862016 MicrobiologicalQuality Assurance and PlantHygiene in the Food andBeverage Industry

This training course in advancedmicrobiology lasts two full dayswith theoretical and practicalsections.

Theoretical Section:• Basic principles and applica-

tion of the HACCP concept• Plant hygiene• Product spoiling

microorganisms• Methods of differentiation

Practical exercises:• Differentiation of bacteria• Practical exercises with the

API 20E and BBL Crystal• Microscopy of bacteria, yeasts

and fungi


Target Group:Staff working in fields of qualityassurance | quality control.

Prerequisites:The participants must be familiarwith the basic principles ofmicrobiology.

116

117

Order Number Index

Order Number Index.

00118 2700124 28, 2900177 6100179 4701029 6101030 6101324 2201325 2211104 7811105 7811106 7811107 7811301 81–8211302 81–8211303 81–8211304 81–8211305 81–8211306 81–8211342 81–8211403 87–8811404 87–8811405 87–8811406 87–8811803 8411806 8411807 8411842 8412602-080 ALK 10413001 8813004 87, 8813005 87, 8813006 87, 8813101 8813106 87, 8813207 C4G 6813209 E 6813229 E 6813239 E 6813249 E 6813269 E 6813279 E 6813400 8613420 8613430 8613440 8613806 8813906 8814003-.... 8914005-.... 8914006-.... 8914008-.... 8914051-.... 89–9014053-.... 89–9014055-.... 89–9014056-.... 89–9014057-.... 89–9014058-.... 89–9014059-.... 89–9014061-.... 89–9014062-.... 89–9014063-.... 89–9014064-.... 89–9014065-.... 89–9014066-.... 89–9014067-.... 89–9014068-.... 89–90

14069-.... 89–9014070-.... 89–9014072-.... 89–9014074-.... 89–9014075-.... 89–9014078-.... 89–9014079-.... 89–9014080-.... 89–9014082-.... 89–9014083-.... 89–9014084-.... 89–9014085-.... 89–9014086-.... 89–9014087-.... 89–9014089-.... 89–9014090-.... 89–9014096-.... 89–9014097-.... 89–90141... 26, 9115406... 8015407... 8015458... 8016201 3016214 2116219 3016220 3016249 47, 5316251 6116254 6116274 49, 5316275 48, 5316276 5016277 5116286 10616296 10516306 2716307 2816309 2916315 2716316 2816401-.... 3116402... 3116403-.... 3116407-.... 3116413 3716464 3716506 7616508 B 45, 5316510 2516511 2516514 E 20, 2516517 E 2116520 C 7616522 3716523 3716532 1816533 1816534 K 1816534 Q 1816537 1816540 4616541 1816553 1816555 K 1916555 Q 1916574 20

16579 4616592 K 1916592 Q 1916596 HYK 6016596 HYQ 6016606 3716610 3516611 3516612 3616615 3616617 5516623 3416625 3616633 5516634 5716639 2216644 E 2216645 E 2216646 E 2216647 E 2216656 5716660 4816662 5516671 3416672-1 3516673 3516685-2 2216692 3616695 3616696 3716746 10316747 10316748 10316757 10216807 3616823 5716824 3216826 35, 3716828 3216829 3516831 3216832 3216836 3316837 3316863 5416880 5316881 5316931 5516999 57, 5317004 3517005 3517006 3517016 5317017 5317019 5317033 5717036 5717038 4717039 4617051 4617068 6117069 6117070 5617085 10417088 104

118

17089 53, 54, 6117090 5417091 5717098 5317099 5317105 4617108 D 2617109 2617145 4717146 2817147 2817148 2717149 2717150 53, 5717152 7617153 7617170 5417173 3517174 3517175 3517208 10417258 10317521---001 7517521---101 7517521---102 7517528-080 ACD 102, 10317528-080 BZD 102, 10317530 5517531 5517532 5517533 5517534 5517535 5517536 5517558 K 1717558 Q 1717559 K 1717559 Q 1717573 K 1717573 Q 1717574 K 1717574 Q 1717575 K 1717575 Q 1717576 K 1717576 Q 1717593 K 1917593 Q 1917594 K 1917594 Q 1917597 K 1817597 Q 1817598 K 1917598 Q 1917635 5617636 5617649 3417655 10417656 10417657 10417660 10417661 10417662 10417712 4417747 4417748 44

17749 4417750 4417761 K 1617761 Q 1617762 K 1617762 Q 1617764 K 1617764 Q 1617765 K 1617765 Q 1617766 4417771 4417773 4417799 2217801 10317802 57, 6317804 E 6017804 G 6017804 M 3617804 NPE 6017804 NPG 6017805 E 6017805 G 6017805 UPN 6017805 NPE 6017805 NPG 6017820 K 1717820 Q 1717821 K 1617821 Q 1617822 K 1617822 Q 1617823 K 1817823 Q 1817824 K 1917824 Q 1917825 Q 1917829 K 1917829 Q 1918052 D 4018053 D 4018056 D 4018058 D 4018059 55, 531808... 23, 2418080M 2418081E 2418082E 2418083E 2418084E 2418085E 2418086E 2418099 40, 2218113 3418406 7918407 791ZE-0006 8523006 8523007 8524002 2625006 8325007 83305144... 75305146...... 75305186...... 75350019... 63

350019V15-K025A 635181306.... 625181307... 625181358.... 625181506.... 645181507.... 645181558.... 645231306...... 425231307...... 41, 42, 445231507 425441307... 665441507… 665571303… 435571305… 435571503… 655571505… 655591301… 435591302… 435591303… 435591305… 435591342… 435591501… 655591502… 655591503… 655591505… 655591520… 655591542… 655601304... 435601305... 435601504... 655601505... 655621304... 435621305... 435621306... 435621307... 435621504... 655621505... 655621506... 655621507... 65611... 94, 95613... 94, 956980101 306980102 306980103 30, 326980104 306980110 456980115 276980116 276980117 276980119 28, 296980120 286980121 286980123 286980151 47, 496980176 616980178 61, 476980180 61, 476980225 256980226 256980227 256980228 256980229 256980230 256980232 25, 37, 456980233 256980234 25

Order Number Index.

119

Order Number Index

Order Number Index.

6980235 25, 356980236 256980264 326980267 356980268 356980270 356980271 356980272 356980383 456980389 566980390 566980395 566980396 566980407 576980415 566980420 566980569 206980570 216980573 376980574 376980586 376980588 376980589 376980590 376980591 376980595 216980656 48–52, 616980700 466980701 466980702 49, 506980703 466980704 466980705 466980706 466980707 48–506980708 48–506980711 48–506980712 46, 48–506980713 466980714 516980715 516980716 516980717 48–50, 51, 616980718 516980719 516980721 616980722 48–52, 616980737 47, 616980740 506980801 47–49, 616981001 30, 326981002 30, 326981003 30, 326981004 30, 326981031 616981033 616981032 616981034 616981063 30, 326981064 30, 326981065 30, 326981090 256981139 366981288 476981314 576981320 22

6981321 226981322 226981323 226981540 376982001 48–506982002 496982003 616982005 47, 616982006 47, 616982012 486982020 476982022 516982029 576982036 496982043 48, 496982044 516982060 576982061 576982070 48–506982071 48–506982072 48–506982077 516982078 516982079 516982119 30, 326983001 296983002 296983003 296983004 296983005 296983006 286983008 276983009 28, 296983010 286985000 466985001 466985002 466985004 456985010 466985011 466985060 376985093 566985128 54, 576985131 566985149 646985150 646985151 646985183 48–516985184 48–516985216 576986006 556986017 366986055 47, 496986070 226986071 226986073 226986078 226986079 226986080 226986081 226986083 476986084 476986090 576986091 576986092 576986105 34

6986110 566986111 566986112 566986113 566986114 566986115 566986116 566986117 566986118 566986119 566986129 566986130 566986131 566986132 566986133 566986137 566986138 566988093 576988094 557M19LSB0021 527M19LSB0028 6376001052 7276001055 7276001058 7276001059 7276077009 7276077609 7276077616 7276077617 7276077618 7276077619 7276077308 7376077303 7376077306 7376077333 7376077410 7350011436 7350032039 7350034067 73BBI-8535108 100BBI-8530220 100BBI-8531722 100BBI-8533024 100BBI-8535027 100BBI-8864829 99BBI-8804845 99BBI-8864926 99BBI-8864942 99BBI-8865027 99BBI-8865221 99BBI-8865124 99BBI-8865329 99BBI-8865426 99BBI-8865620 99BBI-8865523 99BBI-8865728 99BBI-8860866 98BBI-8860858 98BBI-8862320 98BBI-8862427 98BBI-8862524 98BBI-8862621 98BBI-8863024 98BBI-8863121 98BBI-8863202 98BBI-8863245 98

121

Product Name Index.

Absorbent pads 91AC membranes 87–88Air sampler 102–104All-Glass holder 29Anaerobic container 34Arium 94–95

Biosart 250 Funnels 31Biosart 100 Monitors 31

Calibration unit 104Capsules 42–44,

62Cellulose acetate membranes 78Cellulose nitrate membranes 81–82,

87-89Cellulose triacetate cassettes 68–69Centrifugal units 68–70Centrifuges 101Centrisart-C4 68Centrisart-I 68Certomat shakers 98–99Chemical compatibility 107–112Colony counter 34Combisart 31Crossflow cassettes 75Crossflow systems 71, 75Crossflow units 71Culture media 91

Diaphragm pump 55Disc filters 78–85,

87–89Dosing syringe 22

Flexiperm 73Forceps 36

Gelatin filter units 102–104Glass fibre filters 86Glass holders 27, 28, 37Gray membranes 87–89Green membranes 88–89Gridded membranes 87–89

Hand vacuum pump 35Homogenizers 100Housings 52, 63Hydrophobic edge membranes 87–88Hydrophobic membranes 84Hydrosart cassettes 75

Incubator 34Integrity testers 22,

105–106

Manifolds 31–33, 35, 37

MD8 Air Sampler 103Membrane Adsorbers 113–114Membrane filters 78–85,

87–89Midisart 2000 60Mini Cartridges 64, 65Mini Cartridge housings 63Minicheck-BP 22Miniperm 72Minisart syringe units 16–19Minisart-GF 19Minisart-HY 60Minisart-plus 18–19Minisart-RC 16Minisart, standard 0.2 µm 18Minisart, standard, 0.45–5 µm 19Minisart-SRP 17

Needles 22Nitrocellulose membranes 81–82Nutrient Pad Sets 89–90

Peristaltic pump 37, 36Petriperm 73Polyamide membranes 83Polycarbonate holders 20–21,

25, 45Polycarbonate membranes 85Polyester separators 86Polyethersulfone cassettes 68–69Polyethersulfone membranes 80Prefilters 36, 86Prefilter attachment 36Prefilter Capsules 43Prefilter Mini Cartridges 65Pressure filter holders 45–52Pressure filtration units 40–44Pressure hose 57Pressure tanks 54–56PTFE holders 20, 46PTFE membranes 84Pumps 55, 37

Quadriperm 73Quick connectors 57

RC membrane filters 79

Product name Page Product name Page Product name Page Product Name Index

Product Name Index.

Sartobind discs, modules 113Sartobran 150 41Sartobran 300 Capsules 41Sartobran-P Capsules 42Sartocheck 3 106Sartocheck Junior BPplus 105Sartoclean-CA Capsules 43Sartoclean-CA Mini Cartridges 64Sartoclean-GF Capsules 43Sartoclean-GF Mini cartridges 64Sartocon Slice 75Sartofluor Capsules 62Sartofluor Mini Cartridges 64Sartolab-P20 40Sartolab-P20plus 40Sartolab-V units 24Sartopore Capsules 66Sartopore Mini Cartridges 66Sartopure-GF Capsules 43Sartopure-GF Mini Cartridges 64Sartopure-PP Capsules 43Sartopure-PP 2 Mini Cartridges 64School Kit 26Stainless steel holders 20,

30, 32,35,47–52,61

Sterile venting units 60, 62Sterility test system 37Sterisart 37Syringe filter holders 20, 21Syringe filtration units 16–19Syringes 22Suction flasks 35

Three-way valve 22Trainings, seminars 115–116Ultrafiltration units 68–74Ultrasart D20 76

Vacusart 36Vacuum filter holders 25–32Vacuum filtration units 24Vacuum hose 34Vacuum pumps 36Vent filters 62–64Vivacell 70Vivaflow 71Vivapure 74Vivaspin 69

Wash water Capsules 44Water jet pump 35Woulffe's bottle 35

For the purpose of productdevelopment we reserve the right to make changes without notice.

122

Product name Page Product name Page

Specifications subject to change without notice. Printed in Germany on paper that has been bleached without any use of chlorine.W/sart-111a · GPublication No.: SL-0001-e03038Order No.: 85030-500-72

Sartorius AGWeender Landstrasse 94–10837075 Goettingen, Germany

Phone +49.551.308.0 Fax +49.551.308.3289

www.sartorius.com

Sartorius Corporation131 Heartland Boulevard, Edgewood, New York 11717, USA

Phone +1.631.2544249Fax +1.631.2544253Toll-Free +1.800.3687178

Sartorius LimitedLongmead Business Centre Blenheim Road, Epsom, Surrey KT199QN, Great Britain

Phone +44.1372.737100Fax +44.1372.729972

Sartorius S.A.4, rue Emile Baudot91127 Palaiseau, France

Phone +33.1.69192100 Fax +33.1.69200922

Sartorius S.p.AVia dell’Antella, 76/A50011 Antella (FI), Italy

Phone +39.055.634041Fax +39.055.6340526

Sartorius K.K.No. 3 Hoya Building 8–17Kamitakaido 1-chome Suginami-ku Tokyo 168-0074, Japan

Phone +81.3.33295533Fax +81.3.33295543

Sartorius S.A.C/Isabel Colbrand 10-12Edificio Alfa IIIplanta 4, of. 12128050 Madrid, Spain

Phone +34.91.3586100Fax +34.91.3588804

Sartorius Laboratory catalogue Products for research and ...

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Transcript of Sartorius Laboratory catalogue Products for research and ...