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QAM Sampling Recommendations

Recommendation for random and aimed samplingregimes for low acid products.

Random sampling of low acid products

Minimum 50-100 samples from each filler and batch2 consecutive samples should be drawn each time

85-90% of the samples 10-15% of the samples

These samples to be incubatedin 30-35C for min. 5 days

These samples to be incubatedin 50-55C for 3-5 days

EvaluationH, visual, sensoric

Evaluation pH, visual, sensoric

Defect No Defect No DefectDefect

Production put on hold.Plate the defect samples (streak on agar).Incubate the plated samples in itsrespective incubation temperature rangefor minimum 3-5 days

Rough identification ofSpoilage organism/sTrouble shootingCorrective actionTest run

Product can be released.Plate (Streak on agar)5-10% of above samplesas verification andincubate acc. abovemention temperature for5 days.

Product can be released.Plate (Streak on agar)5-10% of above samplesas verification andincubate acc. abovemention temperature for3-5 days.

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QAM Sampling Recommendations

Aimed sampling of low acid productsTwo (2) samples should be drawn directly after every event;

Short stop re-start

Reel splice

Strip splice

End of production

These samples should be incubated in 30-35C for min. 5 days.

Evaluation: Visual, pH and Sensoric. If defect or deviations is found the sample/s should be plated (streak on agar) for RID.

If deviation is detected should investigations on filling machine condition and operation, personal hygiene and operators health condition depending on result of the RID

Start up samples of low acid productsIf a filler has been idle for longer time like a weekend or similar is it strongly recommendable todraw 15 samples directly at start-up time and to be incubated in 30-35C for min. 5 days.

Evaluation: Visual, pH and sensoric. All the samples should be plated and incubated in 30-35Cfor 3-5days for verification of present of bacteria.

If any deviation or growth is detected should investigations on Hygiene, CIP and pre-sterilisation

and corrective actions shall take place.

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QAM Sampling Recommendations

Recommendation for random and aimed samplingregime for high acid products.

Random sampling regime of high acid productsMinimum 50 100 samples is drawn for each filler and batch

All samples to be incubated in 20 25C for 5 7 days.

Evaluation: Visual and sensoric

Blown packages commonly due to Yeast, sometimes/rare other like Lactobacillus.

Sensoric deviation commonly due to Mould (fungi).

Aimed samples regime of high acid productsTwo (2) samples should be drawn directly every event;

Short stop re-start

Reel splice

Strip splice

End of production

These samples should be incubated in 20-25C for min. 5 days.

Evaluation: Visual and Sensoric.

If defect/s is/are found same investigation as for low acid

Start up samples of high acid productsIf filler has been idle for longer time like a weekend or similar is it strongly recommendable todraw 15 samples directly at start-up and to be incubated in 30-35C for min. 5 days.

Evaluation: Visual, pH and sensoric. All the samples should be plated (pour plate) in OrangeSerum Agar and incubated in 30-35C for 3-5days for verification of present of Lactobacillus.

If any deviation or growth is detected should investigations on Hygiene, CIP and pre-sterilisationand corrective actions shall take place.

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