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QIAgility ─ Pure Precision

Andrea TesorieroApplication Specialist

QIAGEN 2009

Sample & Assay Technologies

First we automated manual spin column

preps…

You Have More Important Things To Do….

Now we are able to automate all manual

pipetting in the lab…

20092007

Now QIAGEN takes the next step and enables another convenience shiftNow QIAGEN takes the next step and enables another convenience shift

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From Manual to Automation

Higher Precision and more time every day for…

Preparing & designing new experiments

Data analysis

Literature search

Writing scientific articles

And much more…

Eliminating tedious pipetting in the lab!

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PrecisionResults achieved exceed the best hand pipetting

VersatilitySupports all different cycler formats in the market

Ease-of-useWhat-you-see-is-what-you-get software interface

Speed

Streamlined workflow by

Developed For PCR SetupQIAgility Main Advantages

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QIAgility performs all “tricks” an experienced technician uses

while pipetting

Dynamic liquid level sensing limiting tip immersion

Tracking the meniscus in each tube to minimize tip

retention

Retracting the tip while dispensing

Minimizing Tip/Liquid contact

Slowing down pipetting speed for viscous liquids

Avoiding formation of bubbles in tip

Selecting automatically which tip (50µl or 200µl) to select

maximizing precision

High Precision Meets Pipetting SkillsPrecision Aspects

Precision

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No matter what real-time instrument you use, QIAgility is

compatible

The system is pre-configured to work with all PCR tube

types and all plate formats, including varieties of 96-

well, 384-well and Rotor-Disc

Beside any PCR setup the system can perform

Set-up of quantification standards (dilution series)

Normalization of sample concentration

Sample pooling

Transfer of samples from one tube format to

another

Automatic set up for multiple variables

Post PCR setup

The Hallmark Of QIAgility Is Its VersatilityVersatility Aspects

Versatility

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VersatilityQIAgility supports all real-time PCR formats

LightCycler™ CapillariesRotor-Gene™ 0.2 mL, 0.1 mL and

Gene-Disc™ formats

SmartCycler™ Tubes

Roche COBAS™

96-well and 384-well(Incl. all ABI, Bio-Rad, Stratagene, Eppendorf etc. instruments)

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Versatility96 and 384-well support

Compatible with plates, tubes, tube racks, cooling blocks etc

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VersatilityUnusual formats are also supported

Including: MALDI plates, ELISA plates, reservoir plates, etc, etc

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The Intuitive Software Is Easy To UseSoftware Features

The software mirrors the actual deck setup

Intuitive layout—simple to learn

Calculation of reagent needs according to the

number of reactions and replicates specified

Tip re-use options

Import/Export of sample names

Pre- and Post-run reports including worktable

setup guide

Virtual mode aids new protocol testing

Wizards for speedy PCR setup and optimization

Ease of Use

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You Have More Important Things To Do….QIAgility Means Time Savings

“If you worked once with the QIAgility, you don’t

remember how you have managed without…”

Staff can be attending to other duties while the

QIAgility is running

Simple Transfer Actions 1:1

– 96 samples

• 17 minutes

• 6 minutes with tip reuse

Transfer Actions with pre-MM

– 96 samples PCR set-up

• ~25 minutes

Transfer Actions with pre-MM

– 192 samples PCR set-up

• ~46 minutes

Speed

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Unrivaled Pipetting PrecisionRotorGene Probe RT PCR Kit

Data presented in brochure table 1

Precision

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Exceptional Reproducibility and Accurate ResultsRotorGene Probe PCR – Template dilutions

Setup Details

Target: IL1 R2

Sample: Hs gDNA

10 twofold dilutions

(30ng – 60pg)

5 replicates

Rotorgene 6000

Data presented in brochure figure 1

Precision

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Automated Workflow for Accurate ResultsRotorGene Probe RT PCR Kit

Setup Details

Target: IL1 2RB1

Sample: Leuko RNA

Standard row in triplicates

5 tenfold dilutions (10 pg – 100 ng)

Rotorgene 6000

Data presented in brochure fig 3

Precision

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Worktable setup example

Worktable Positions

1. Master Mix Block

2. Reagent Block

3. Tips

4. Tips

5. Tips or Rotor-Gene Q Loading Block

6. Tips

7. Sample Plate or Rotor-Gene Q Loading

Block

8. Reaction Plate

1

2

3 4

5 6

7 8

1

2

3 4

56

7 8

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Guides you through creating a

run file with master mix, standard,

Sample and final reaction!

Life Can Be So Simple….QIAgility PCR Setup Wizard

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Just a few simple steps

Define Plate/Tube Format Sample Bank (number of

samples) Reagents (primer, buffer, taq,

etc.) Master Mix (pre-mix, setup) Standards Reactions

Add Controls Add Standard Add Samples

GO!

Set Up Your Own RunSoftware Explanation

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N View the software