Salvatore Valenti
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![Page 1: Salvatore Valenti](https://reader036.fdocuments.net/reader036/viewer/2022062307/554e9b83b4c90573338b53fe/html5/thumbnails/1.jpg)
Isolation and Transmission Electron Microscopic Characterization of
Bacteriophages in Sewage Specific for E.coli O157:H7 and Citrobacter Freundii
Salvatore Valenti-MicrobiologyDepartment of Biological Sciences
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![Page 3: Salvatore Valenti](https://reader036.fdocuments.net/reader036/viewer/2022062307/554e9b83b4c90573338b53fe/html5/thumbnails/3.jpg)
My Question?
• Can I prove that phages are present in the sample collected from a sewage plant by visualizing them via Transmission Electron Microscopy?
• By answering this question I will help future generations of Wagner microbiology students perform further analyses of bacteriophages.
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Philips CM 100 Transmission Electron Microscope
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E.Coli O157:H7 phage from my sample
A B
C D
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Citrobacterphage from my sample
A B
C D
![Page 7: Salvatore Valenti](https://reader036.fdocuments.net/reader036/viewer/2022062307/554e9b83b4c90573338b53fe/html5/thumbnails/7.jpg)
Conclusion
• Water samples from sewage plants are rich in phages for E. coli O157:H7 and Citrobacter freundii as it was proven with electron microscopy.
• Having learned the techniques to isolate bacteriophages and visualize them with the electron microscope makes me more competitive in the job market.
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Acknowledgements
• Dr. Kathleen Bobbitt• Prof. Christopher Corbo• Zulmaire Franco• Dr. Roy Mosher• Dr. Adam Houlihan