SALMONELLA SALMONELLA TYPHIMURIUM- GETTING TYPHIMURIUM- GETTING TO THE BOTTOM OF ITTO THE BOTTOM OF IT

G. Doran , N. DeLappe,

C. O Hare, G. Corbett – Feeney,

M. Cormican

National Salmonella Reference Laboratory, Galway

INTRODUCTIONINTRODUCTIONS. TYPHIMURIUM COMMON IN

EUROPE, USA

POULTRY, CATTLE & PIGS MANY FOODS

MULTIDRUG RESISTANT ISOLATES

INTRODUCTIONINTRODUCTION -2 -2 MULTI DRUG RESISTANT

DT104

INCIDENCE INCREASING ACSSuT – CHROMOSOMALLY

CARRIED TRIMETHOPRIM, NALIDIXIC

ACID RESISTANCE SUSCEPTIBILITY TO

CIPROFLOXACIN

TYPING METHODSTYPING METHODSPHAGE TYPING

ANTIMICROBIAL SUSCEPTIBILITY

PLASMID PROFILING

PFGE

PRINCIPLES OF PFGEPRINCIPLES OF PFGE

CATTTTACGTAAAATG

PRINCIPLES OF PFGEPRINCIPLES OF PFGE

CATGTTACGTACAATG

NO CUT

PULSENETPULSENETSTANDARDISED PFGE

CDC USA

E. coli, Salmonella, Listeria, Shigella

CENTRAL DATABASE

PFGEPFGE

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

PLASMIDPLASMID PROFILE PROFILE1 2 3 4 5 C C C C 6 7 8 9 10

Size (MDa)

125

26

10

2

SS. TYPHIMURIUM 2001. TYPHIMURIUM 2001

303 ISOLATESANIMAL AND HUMAN ORIGIN

PHAGE TYPING (N = 303)ANTIMICROBIAL SUSCEPTIBILITY

(N = 303)PGFE TYPING (N = 163)PLASMID PROFILING (N = 36)

S. Typhimurium Phage Types

DT104

DT104b

DT193

U310

U302

DT132

Other

ANTIMICROBIAL ANTIMICROBIAL SUSCEPTIBILITYSUSCEPTIBILITY

0

20

40

60

80

100

1 2

Resistance Pattern

% R

esis

tanc

e

Non MDR

Other MDR

ASSuT

ACSSuTSp +

ACSSuTSp

PLASMID PROFILINGPLASMID PROFILING36 ISOLATESMODIFIED METHOD OF KADO

AND LIU

PLASMIDS 1.5 - 125 MDA60MDA PLASMID IN DT104, MDR

DT120 AND DT12

A

B

C

D

E

F

G

H

MDRPATTERN PHAGE TYPE

DT104, DT104b, MDR DT12 &12088% MDR

32 ISOLATESFULLY SENS

DT12

DT120

PHAGE TYPE PHAGE TYPE U310U310

EMERGED IN 2001 PIGS, HUMANS15 ISOLATES MINOR DIFFERENCES

ON PFGE

DISCUSSIONDISCUSSION S. TYPHIMURIUM –

HUMANS & ANIMALS MDR - INCREASED

DT104 & DT104b MAJORITY MDR, CLONAL

DISCUSSION - 2DISCUSSION - 2 DT104, DT104b

PFGE U302, RDNC

P11 MDR DT12, DT120

P3, P5,P7, P14 DT193P18, P20P23, P27

CONCLUSIONSCONCLUSIONSPHENOTYPIC & GENOTYPIC

METHODS NECESSARY

HIGH INCIDENCE OF MDR IN IRELAND

CONTINUOUS MONITORING OF S. TYPHIMURIUM NECESSARY

OTHER RESEARCHOTHER RESEARCH

FLUROQUINOLONE RESISTANCE IN S. ENTERITIDIS– DONNA KILMARTIN