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WELCOME TO

A PRESENTATION ON CLINICAL PATHOLOGY OF VESICULAR

EXANTHEMA

SUBITTED BY ROLL: 10/49

REG. NO: 527GROUP: B

SUBMITTED TODR. SHAFIQUL ISLAM MAMUN

LECTURERDEPARTMENT OF PATHOLOGY

AND PARASITOLOGY

Chittagong Veterinary & Animal Sciences University,

Khulshi - 4225, Chittagong.

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MVPK cell cultures was done. Cytopathic effect was seen in cell line.1. Cell Culture

2. Animal Inoculation

Rectal temperatures increases within 24 hours of inoculation. Vesicular Lesions appeared at inoculation sites by 48 h PI or if the first vesicle on an animal Appeared at a primary site by 72 hPI and a febrile response preceded the Lesion by no more than 24h.

Cytopathic effect

Reff:Veterinary Medicine by Douglas C. Blood et al.

CLINICAL PATHOLOGY OF VESICULAR EXANTHEMA

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Serological test

Complement Fixation test

A standard CF test (15) was done, round-bottom, plastic, microtiter plates. Four hemolytic units of complement were used in the test and antigen titrations were conducted in triplicate. Antigen was reacted for 30 min with diluted test sera at 370 C. Sensitized sheep red blood cells were incubated with the reactants for 45 min at 37°C with agitation. Plates were centrifuged at 600 x g for 5 min prior to reading the amount of hemolysis.

Serum neutralization tests were conducted using the 12 convalescent antisera to VES virus. Virus neutralization indices (VNI) were calculated by using the system Used for SN but with a constant1/10 Serum dilution and varying tenfold Dilutions of virus. Neutralization indices for the sera were expressed as logio of virus neutralized.

Serum neutralization test

Reff: Advances in veterinary science. New York: Academic

Reff:MADIN SH. Pathogenesis of vesicular exanthema of swine. PhD Thesis, University of California,

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RT- PCR

Agar gel precipitation test

Reff:Bankowski, R. A. 1965. Vesicular exanthema. Adv. Vet. Sci. 10: 23-64.

REFERENCES

Veterinary Medicine by DOUGLAS C. BLOOD et. al.Principles of bacteriology, virology and immunity by sir graham S wilson and sir ashley.Introduction of modern virology by n.J.DimmockVeterinary bacteriology and virology by I.A. Merchant and R.A. Packer. Bankowski ra. Vesicular exanthema. In: brandly ca, jungherr el, eds. Advances In veterinary science. New york: academic Press, 1965; 10:23-66. Madin sh. Vesicular exanthema. In: Dunne HW, leman AD, eds. Diseases of Swine, 4th ed. Ames, iowa: the iowa state University press, 1975: 286-307.

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THANKS TO ALL