S2 Brochure-100-067-001 RevA WebViewing€¦ · S2 Genomics provides a selection of automated...
Transcript of S2 Brochure-100-067-001 RevA WebViewing€¦ · S2 Genomics provides a selection of automated...
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Tissue to single cells or nuclei in minutes.Singulator™ system.
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Single-Cell Tissue Prep Automated
Singulator™Single-useCartridge
Mouse small intestinenuclei in ~ 7 minutes
Mouse kidney cellsin ~30 minutes
Cells Nuclei
Fresh, Frozen, OCTFreshTissue Type
20,000 - > 600,000/mgYield*
7 minutes20-60 minutesProcess Time
N/A70 - 95%Viability
* Varies depending on tissue types
Lung Kidney Liver Spleen Intestine
High Cell Viability
94%78%
91% 95%
76%
(1:10)
Lung Kidney Liver Spleen Intestine
High Cell Yield (Titer/mg x1000)
6576
28
121
29
(1:10)
The bench-top Singulator System and its single-use cartridge enable rapid and hands-off tissue dissociations into singulated cells or nuclei suspensions. Researchers can now easily and reproducibly obtain suspensions of high viability cells or nuclei, for a wide range of single-cell analyses.
10,000 - > 500,000/mg
A merged view of DAPI and brightfield images of nuclei extraction from flash frozen mouse tissues using the Singulator 100. Image: Courtesy of Dr. Minoda’s Lab, Epigenome Technology Exploration Unit, RIKEN, Japan.
High Speed. High Yield. High Viability.
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Minimal Variability. Multiple Applications.
OnePlatform
Gen
om
ics
Cel
l Bio
logy
scRNA-Seq
snRNA-Seq
ATAC-Seq
CITE-Seq
Immuno-oncology
Isolate& Purify
Cells
FACS
• Single nuclei in 7 minutes
• Single cells in ~ 30 minutes
Fast Processing
• 70 - 95% viability
• 20,000 - 600,000 nuclei/mg(tissue dependent)
High Yields &Viability
• Load tissue and press RUN in < 1 minute
• Intuitive touch-screen menu and control
• Minimal operator training
Simple Setup.Walk-awayOperation
• Consistent results, from researcher to researcher and lab to lab
• Ensure success with precious samples
• Minimize transcriptome changes
• Customize protocols and use your reagents for your tissues of interest
ReproducibleResults
TissueCollection
TissuePrep
Single CellProcessing
LibraryPrep
Next-genSequencing
Analysis
Reduce hours of challenging manual steps into minutes of work on the Singulator System
Ideal for genomics, cell biology and other ‘omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-seq, FACS, and immuno-oncology.S2 Genomics provides a selection of automated pre-set protocols andpre-formulated reagents for cell isolations from an expanding set of mouse, rat, and human tissues and tumors – and for nuclei isolations from mostmammalian tissues, and some insects and plants. Create your own protocols and use your reagents of choice for your tissues of interest.
T ypical yield from mousetissue:
• 10,000 - > 500,000 cells/mg
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Complete, flexible, and standardizablesolution for your single cell research.Intuitive Software with FlexibilityChoose from standard protocols or customize processing parameters, including enzyme incubation time, temperature,mixing profile and mechanical disruption profile.
1 2
3 4Input user notes orpress RUN
The progress monitor indicatesremaining time of the run
SINGULATOR SYSTEM PRODUCTS
Singulator™ 100 System
Singulator™ Nuclei Isolation Kit (25-samples pack)
Singulator™ Cell Isolation Kit (25-samples pack)
Singulator™ Chilled Reagent Station
Part No. 100-067-001 RevA
100-058-656
100-060-817
Inquire for your specific tissue requirements
100-060-926
CATALOG NUMBER
S2 Genomics • 6712 Preston Avenue, Ste D • Livermore, CA 94551
Tissueto singlecells ornuclei inminutes.
www.S2Genomics.com
For research use only. Not for use in diagnostic procedures. S2 Genomics makes no representation or warranty as to the usefulness, completeness, or accuracy of the information contained in this brochure. The products, services, and specifications set forth in this brochure are subject to change without notice and S2 Genomics disclaims any and all liability for such changes. The information contained herein is provided without warranties of any kind, either express or implied, and S2 Genomics disclaims any and all liability for typographical, printing, or production errors or changes affecting the products, services, and/or the specifications contained herein. It is the responsibility of the customer to thoroughly analyze all aspects of the customer’s proposed application for the products and services. Singulator™ is a trademark of S2 Genomics.
Select your tissue prep process from the list of Standard, Favorites or Recently Run protocols
Step-by-step instructions and videos guide you throughthe setup