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Electronic supplementary material (table S1 and figures S1-S7)
Table S1. Primers for making ISH probes.
Gene name Forward primer (5' → 3') Reverse primer (5' → 3') Product length (bp)
Bat_Hoxd9 TACGAGGTGGCAAGGATT TTCCAGGTCTCAGTCAAGG 629
Bat_Hoxd10 AGTAGATGTCGCTGTTGTCCGC AAGGGACCTCGGCAGAAATG 602
Bat_Hoxd11 TTCTTCCCACGGTCAACT GCAGCACCAGGATCACAT 607
Bat_Hoxd12 TTTCGTCCAATCGTCTGA CCTTGGAGTCGTCCTTGA 587
Bat_Hoxd13 ACATGGTGTCCACCTTCG AAAGTCAGGTTGGGTAGCA 590
Bat_Tbx3 TCCTCAACCTGAACACCAT AGATCCCGGATACAGAAACC 543
Bat_Fam5c GCGGAACCTGGGCTACAT AAGCATTGCCACGGAACA 630
Mouse_Hoxd9 GCCAGGATTCTGAACCTTACA GAGGCTGGATAGGGTGAGAC 511
Mouse_Hoxd10 CCGAGTCTTGTCCTGTTG GGTTTGTTTCCGCCTTGC 705
Mouse_Hoxd11 CTCGGATGCTCAACCTCAC ACAGTGACTCATGCCCAAAG 556
Mouse_Hoxd12 TGAGCCCGTCCTCCTATT CCACTTGCACTGCCATGTTC 599
Mouse_Hoxd13 CACTTCGGCAACGGTTACTA CCTTCACCCTTCGATTCTG 607
Mouse_Tbx3 GAAGTCAGGAAGGCGAATG TGGGCAAAGCAGTTGAAGG 609
Mouse_Fam5c CTGGGAAACAAATGGAAGA CAAGATTTTGGGTTGTGCT 493
Figure S1. Alcian blue staining of bat and mouse autopods. Orientation of limbs as in figure 1. F:
forelimb; H: hindlimb; S16E: early stage 16; S17M: middle stage 17; S19L: late stage 19. Scale
bars, 500 μm.
Figure S2. Hoxd10 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
Figure S3. Hoxd11 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
Figure S4. Hoxd12 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
Figure S5. Hoxd13 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 500 μm.
Figure S6. Tbx3 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
Figure S7. Fam5c expression in the fore- (F) and hindlimbs (H) of embryonic bats visualized by
ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-H) and 500 μm in (I-P).