Rotary Microtome CUT 4060

microTec

microTec

GmbH - Company for Manufacture and Export of Laboratory Equipment 1

Rotary Microtome CUT 4060

Operating Manual

MADE IN GERMANY

microTec Laborgeräte GmbH Rudolf-Diesel-Str. 47 69190 Walldorf, Germany Phone: + 49 6227 8222-0 Fax: + 49 6227 8222-30 [email protected] www.micro-tec.de

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microTec


Table of Contents 1. General Notes ............................................................................................................. 4 1.1 Significance of the Operating Instructions .................................................................. 4 1.2 Usage According to its Specification ........................................................................... 5 1.3 Improper Use .............................................................................................................. 6 1.4 Warranty and Liability ................................................................................................. 6 1.5 Warranty ..................................................................................................................... 6 1.6 Brief Discription of Use, Function and Sequence ....................................................... 7 1.7 Edition, Date of Issue of the Operating Manual .......................................................... 8 1.8 Copyright .................................................................................................................... 8 1.9 Address of Manufacturer ............................................................................................ 8 2. Basic Safety Instructions ......................................................................................... 9 2.1 Heed the Notes and Instructions Given in this Operating Manual .............................. 9 2.2 Obligation on the Part of the Operator ........................................................................ 9 2.3 Obligations on the Part of the Operating Staff ............................................................ 9 2.4 Hazards and Dangers in Handling and Using the Instrument ................................... 10 2.5 Interpretation of Hazard Symbols ............................................................................. 11 2.6 Informal Instructions Issued by the Manufacturer ..................................................... 12 2.7 Particular Zones of Danger ....................................................................................... 12 2.8 Servicing and Fault Clearance .................................................................................. 13 2.9 Structural Modifications and Replacements to the Instrument .................................. 13 2.10 Cleaning and Associated Care ................................................................................ 14 3. Transportation and Storage ...................................................................................... 15 3.1 Dimensions and Weights .......................................................................................... 15 3.2 Instructions for Transportation .................................................................................. 15 3.3 Packaging ................................................................................................................. 15 3.4 Inspection upon Acceptance by the Recipient .......................................................... 16 3.5 Reporting and Documenting Transportation Damage ............................................... 16 3.6 Ambient Conditions ................................................................................................... 16 4. Data and Information Concerning the Instrument ............................................... 17 4.1 Designation ............................................................................................................... 17 4.2 Scope of Supply ....................................................................................................... 17 4.3 Certification of Conformity ......................................................................................... 18 4.4 Ambient Temperatures during Operation .................................................................. 19 4.5 Performance ............................................................................................................. 19 5. Installation, Assembly, Commissioning ............................................................... 20 5.1 Installation ................................................................................................................ 20 5.2 Transportation Locking Pieces and Securements .................................................... 20 5.3 Controls, Indicators and Displays ............................................................................. 21

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microTec


6. Operation ................................................................................................................ 22 6.1 Preparation, Orientation and Alignment of Specimen .............................................. 22 6.2 Commencing the Cutting Operation ......................................................................... 22 6.3 Instructions for Producing Sliced Microtomic Sections of the Specimen .................. 22 6.4 Cutting Action .......................................................................................................... 23 6.5 Handwheel ............................................................................................................... 23 6.6 Coarse Advanced Drive ........................................................................................... 23 6.7 Friction Clutch on the Coarse Advanced Drive Mechanism ..................................... 24 6.8 The Handwheel of the Coarse Advanced Drive Mechanism (11) ............................ 24 6.9 Collision Protection Device ...................................................................................... 24 7. Setting Up and Changing the Setup ..................................................................... 25 7.1 Specimen Fixture ..................................................................................................... 25 7.2 Standard Specimen Clamping Fixtures ................................................................... 25 7.3 Orientable Adapter ................................................................................................... 26 7.4 Fixed Adapter .......................................................................................................... 26 7.5 Cylindrical Specimen Insert and Prism .................................................................... 27 7.6 Film (Thin Material) Clamping Fixture ...................................................................... 28 7.7 Universal Cassette Clamping Fixture ....................................................................... 29 7.8 Specimen Orientation Device .................................................................................. 30 7.9 Exchanging the Specimen Clamping Systems ........................................................ 31 8. Setting up the Knife ............................................................................................... 32 8.1 Knife Holder ............................................................................................................. 32 8.2 Knife Holder Base .................................................................................................... 32 8.3 The “R“ type Knife Holder ........................................................................................ 33 8.4 The “RS“ type Knife Holder ...................................................................................... 34 8.5 Holder for Throw-away Blades ................................................................................ 35 8.6 Clearance Angle Adjustment ................................................................................... 36 9. Additional Functions ............................................................................................. 37 9.1 Additional Extras on Model CUT 4060 ..................................................................... 37 10. Emergency Service, Customer Service ............................................................... 37

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microTec


1. General Notes

1.1 Significance of the Operating Instructions Dear Customer, Please read through the operating instructions carefully before using the equipment and familiarize yourself with its functions and controls. This is imperative to ensure proper use of the equipment. Please check the production serial number against the number given on the microTec identification plate of your unit. It will facilitate faster service and response to queries by quoting the production serial number.

microTec Production Serial-Number: 60-_______

microTec Laborgeräte GmbH Rudolf-Diesel-Str. 47 69190 Walldorf, Germany Phone: + 49 6227 8222-0 Fax: + 49 6227 8222-30 [email protected] www.micro-tec.de

microTec

microTec


1.2 Usage According to its Specification The CUT 4050, CUT 4055 and CUT 4060 rotary microtomes are manually operated instruments for use in all work involving paraffin wax and solid cut microtomology within the disciplines of biology and medicine, and within industry at large.

The specimen can be moved back and forth by means of the coarse wheel mechanism so as to attain rapid advance of the specimen in relation to the knife during cutting. The micro-drive mechanism enables feed settings to be made within the range of 0,5 to 60 microns. The user friendly throw-away blades and universal knife holder with its integrated knife guard enables trouble-free clamping and setting of the knife blade. The integrated safety device to prevent any collisions occurring, which is incorporated in the micrometer mechanism, disables any adjustment to the feed setting being made upon reaching its maximum setting.

To prevent damage occurring to the knife or the specimen, the rotary microtome CUT 4055 and CUT4060 imparts an action of slight retraction of the specimen by approx. 80 microns during the motion of advancement. The CUT 4060 rotary microtome is equipped with an electronic cutting counter, which indicates the total number of cuts made. A three-position trimming assembly is also incorporated (10, 20 and 30 micron trimming).

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microTec


1.3 Improper Use

The rotary microtome is designed and manufactured to the latest state-of-the-art technology and to recognized principles of technical and engineering safety. Nevertheless, dangers can arise during its use to endanger life and limb of the operator or third persons or damage to the instrument or to other property. The instrument is only to be used

• for use according to its specification of intended use

• in a proper technical state of repair, free of any defects

1.4 Warranty and Liability

The company – microTec GmbH – certifies that this instrument has been carefully tested and inspected. Verification to ensure adherence to the technical specification data published has been carried out prior to shipping out the instrument from the factory. No liability is assumed for the correctness of technical data and other data or drawings. Reserve technical changes.

The instrument is assigned to Class 4 of the MedGV (legislation governing medical supplies). Observance of existing legislation and regulation is assured.

1.5 Warranty

Warranty is provided for this microTec product in the event of any deficiencies or defects in material or workmanship for a period of 12 months. Defective parts will be repaired or replaced free of charge during this period of time. Any additional claims made under the warranty of whatsoever nature are excluded. Neither does the warranty provide cover for any claims arising from services and supplies provided by third parties.

In principle, our “General Conditions of Sale and Supply“ shall apply. These conditions are made available to the prospective operator of the instrument, by, at the latest, the signing of the contract. Warranty and liability claims for personal injury and material damage are excluded if attributable to one or more of the following causes:

• Improper use of the instrument!

• Incorrect installation and assembly, commissioning, operation and servicing of the instrument!

• Operating the instrument with the safety devices defective or safety mechanisms and guards inappropriately fitted!

• Disregarding instructions given in the operating manual concerning transportation, storage, installation, commissioning, servicing and equipping of the instrument!

• Unauthorized tampering and structural modification to pneumatic, electrical and mechanical components!

• Ineffective or incorrect monitoring of machine parts which are subject to wear!

• Improperly effected repairs!

• Catastrophes caused by external influences and force majeure!

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microTec


1.6 Brief Description of Use, Function, and Sequence

Use

The CUT 4050, CUT4055 and CUT 4060 rotary microtomes are manually operated instruments for use in all work involving paraffin wax and solid cut microtomology within the disciplines of biology and medicine, and within industry at large.

Function and Sequence

Manually rotating the hand wheel on the rotary microtome translates into vertical movement of the fixture holding the specimen. To produce the cut, resharpenable knives or throw-away blades are used, which are adapted and attached to the various knife holders. A microtomic cut is made during the downward motion of the specimen (cutting action). By continuing to rotate the hand wheel further in a clockwise direction the specimen starts to rise again in its return motion. The cutting feed can also be set by means of the three-position trimming assembly (only available on the CUT 4060 model). The selected feed for the thickness of cut is instigated at top dead center. Rapid advance of the specimen towards the knife is achieved by means of the coarse feed mechanism. A universal clamping fixture for holding a variety of specimens is provided. As a result, simple alignment to the plane of cut is possible using the facility for orientating the specimen. The various knife holders enable trouble-free use of a variety of knife types and thereby provide facility for making adjustment to suit the application in question.

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microTec


1.7 Edition, Date of Issue of the Operating Manual Edition: Version 004.96 Date of issue: 13. February 1996

1.8 Copyright Copyright : Firma microTec GmbH Copyright to this operating manual remains with microTec GmbH. This operating manual is only intended for use by the operator and his staff. It contains specifications and instructions, which may not be

- copied, - distributed or - otherwise divulged to others in their entirety or in part.

Infringements can lead to legal proceedings being brought against the perpetrators.

1.9 Address of the Manufacturer microTec Laborgeräte GmbH Rudolf-Diesel-Straße 47 69190 Walldorf, Germany Phone: + 49 6227 82 22-0 Fax: + 49 6227 82 22-30 [email protected] www.micro-tec.de

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microTec


2. Basic Safety Instructions

2.1 Heed the Notes and Instructions Given in this Operating Manual!

A prime prerequisite for safe handling and malfunction-free operation of this instrument is the awareness and recognition of basic safety instructions and regulations. This operating manual contains the most important instructions for operating the instrument in a safe manner. This operating manual, especially the instructions on safety, are to be heeded by all personnel who operate the instrument. In addition, compliance with the regulations and specifications on accident prevention applicable for the site of installation and use is to be maintained.

2.2 Obligation on the Part of the Operator The operator is obliged to only allow personnel to operate the instrument, who

• are familiar with basic regulations on safety at work and accident prevention, and are trained in the use and operation of the instrument.

• have read the chapter on safety and the warning notes and instructions given in this operating manual.

• have been taught to have due regard to aspect of safety during the execution of their work.

2.3 Obligations on the Part of the Operating Staff All personnel who are delegated with the task of operating this instrument are obliged before starting work

• to heed the basic regulations on safety at work and accident prevention.

• to read and to comprehend the chapter on safety and the warning notes and instructions given in this operating manual.

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microTec


2.4 Hazards and Dangers in Handling and Using the Instrument

Important! The following precautionary measures must be undertaken during any operation carried out using the instrument. Non-compliance with these precautionary measures will infringe recognized principles and rules of technology and the intended and proper use of the instrument. The manufacturer – microTec GmbH – accepts no liability in the event of such infringement.

Risk of Injury

The finger guard must be used when setting the knife and specimen, in order to reduce the risk of injury on the knife/blade. Always insert the specimen first. Then insert the knife into the knife holder. Before exchanging the clamping device, always remove the knife or blade. Knives, which are not being used, must always be stored in the knife box. Never attempt to catch a falling knife. Take care when cutting or removing a sliced section.

Risk of Infection

Appropriate measures regarding protection and disinfection are to be taken when working with infected material.

Danger due to Radio Activity

The current applicable regulations for the protection against radiation must be observed at all times when working with radio active materials or specimens.

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microTec


2.5 Interpretation of Hazard Symbols

Interpretation

Symbol

• Danger

• Warning

• Caution

• Note

• Important !

• Information

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microTec


2.6 Informal Instructions Issued by the Manufacturer Preparation of the Specimen Due attention should be paid in the preparation of specimens with regard to making the correct choice of the medium used for embedding and encapsulation of the specimen and its curing and infiltration time.

Temperature of the Specimen

Microtomic sectioning is generally carried out at room temperature (except in the case of frozen specimens). Increases in temperature will soften the paraffin wax. The heating up of paraffin wax specimens due to direct exposure to sunlight or their proximity to any other source of heat is to be avoided.

Choosing the Knife

Depending upon the ground facet in question, a clearance setting at an angle of 18 to 20 degrees is to be selected. In the case of re-sharpened knives, use the knife height adjustment facility to set the height of the knife. Levers and Clamping Screws Securely tighten all levers and clamping screws on the knife holder and the specimen orientation device. -> Overtightening could result in damage of knife holder!!

Cutting Speed

Rule: “The harder the material, the slower the cutting speed“.

Starting the Cut

Take care when approaching the knife or the specimen.

2.7 Particular Zones of Danger

Important!

Knives are sharp!

Always set the knife guard in its proper position after completing the operation – even if it’s for a brief moment of time – and make sure it is properly seated.

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microTec


2.8 Servicing and Fault Clearance Servicing To secure the quality of the microtomic section and operational reliability of the rotary microtome, a routine service should be carried out once a year. Servicing should only be carried out by a trained service engineer.

Service Contract

microTec offers a service contract which guarantees that your instrument will remain in a perfect operational condition.

Fault Clearance

Malfunctions, which can adversely affect safety, are to be rectified immediately.

2.9 Structural Modifications and Replacements to the Instrument

• No modifications, conversions or enhancements may be made to the instrument without the approval being given by the manufacturer. • All conversions require written confirmation issued by microTec.

• Replace components which are not in proper working order immediately.

• Only use original replacement and consumable parts and items.

• It cannot be guaranteed that parts sourced from other suppliers are designed and manufactured to meet safety and reliability conditions.

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microTec


2.10. Cleaning and Associated Care Cleaning Depending upon the level of dirt and contamination and upon the frequency of use, regular cleaning and care of the rotary microtome is recommended. The instrument is best cleaned using a mild domestic cleaning agent. Do not use harsh solvents or cleaning compounds, because paintwork and plastic components can be damaged by their use. Always remove the knife and stow in the knife box prior to cleaning the instrument.

Important!

Never place a knife on a table or surface with the cutting edge facing upwards. Thoroughly clean the bearing surfaces and contact faces and the guide of the knife holder. Associated Care When the instrument is not in use, turn back the cylinder by means of the coarse drive mechanism and lock the hand wheel. Cover the instrument with the cover supplied.

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microTec


3. Transportation and Storage

3.1 Dimensions and Weights

Dimensions

Width.…….……………………. 460 mm Depth…….…………………….…460 mm Height..…………………………...280 mm

Weight

Weight (without accessories)……..36 kg

3.2 Instructions for Transportation Lock the handwheel. Remove all knives, knife holders, specimen fixtures and specimens from the rotary microtome.

3.3 Packaging The rotary microtome has been supplied in a special packaging designed for transportation. Do not commission (start-up) the instrument until at least 24 hours have elapsed following transportation. Please ensure the specified procedures are strictly observed!

Transportation crate unopened.

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microTec


3.4 Inspection upon Acceptance by the Recipient Firstly, check that all items ordered are supplied in the transportation crate. Inspect the transportation crate and the rotary microtome for any damage incurred during transit.

3.5 Reporting and Documenting Transportation Damage In the event of damage caused in transit, the manufacturer and the forwarding agent are to be advised immediately in writing.

3.6 Ambient Conditions Storage temperature range +5°C to + 55°C.

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microTec


4. Data and Information Concerning the Instrument

4.1 Designation Rotary Microtome, model CUT 4060

4.2 Scope of Supply, i.e. According to order confirmation.

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microTec


4.3 Certification of Conformity

Declaration of Conformity

Manufacturer: microTec Laborgeräte GmbH Rudolf-Diesel-Str. 47 69190 Walldorf, Germany Product: Serial-No.: 60-________

Person in the Community duly authorized to compile the relevant technical documentation: Walter Ganter - microTec Laborgeräte GmbH

Walldorf,

Place, Date Signature Walter Ganter, Managing Director

Rotary Microtome CUT 4060

We declare under our sole responsibility that our product meets all the requirements of the following directives that are applicable

98/79/EG In-vitro Diagnostic Directive

Classification acc. to IVD-RL 98/79/EWG Other devices

Applied harmonised standards, national DIN EN 292, safety of the machines, Standards or other normative documents equipment and systems

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microTec


4.4 Ambient Temperatures During Operation + 10°C to + 35°C

4.5 Performance Cut thickness range: ………………………………………………………0,5 - 60 microns

Progression: ………………………………………………… 0,5 microns of 0,5 - 2 microns 1 micron of 2 - 10 microns

2 microns of 10 - 20 microns 5 microns of 20 - 60 microns Horizontal specimen movement: ……………………………………………..max. 40 mm Vertical specimen movement: ……………………………............................max. 70 mm Specimen size: Standard ………………………………... 55 x 50 mm Specimen orientation: X-and Y- axes: ………………………………………8° Z-axis: ………………………………………up to 360° Cutter drive: …………………………………………………………manual via handwheel Coarse advance: ……………………………………………………manual via handwheel

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microTec


5. Installation, Assembly, Commissioning

5.1 Installation Protect the instrument from:

• sources of heat such as fan heaters or hot air outlets;

• extremely low temperatures;

• direct sunlight;

• excessive dust;

• moisture or rain;

• mechanical vibration or impacts;

• equipment which contain magnets or generate magnetic fields.

5.2 Transportation Locking Pieces and Securements Remove all locking pieces and adhesive tape used for securement during transportation. Place the instrument on a rigid, vibration-free table or bench. Align the instrument on the table. Lift up the instrument a little at the front.

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microTec


5.3 Controls, Indicators, and Displays Item Description

1 Handwheel for cutting operation

2 Handwheel handle for handwheel 1

3 Knob for releasing the handwheel locking device 4

4 Handwheel locking device

5 Rotary knob for setting the thickness of slice

6 Inspection window for setting and inspecting slice thickness

7 Locking lever for knife holder

8 Lever for trimming adjustment*

9 LCD display (cutting counter)*

10 Reset for LCD display*

11 Handwheel for coarse drive

12 Cylinder

13 Clamping mechanism for knife holder

14 Guide bars for knife holder

Fig. 1

13

7

14

11

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microTec


6. Operation

6.1 Preparation, Orientation and Alignment of the Specimen Prior to starting to cut the specimen, the specimen must be suitably prepared and embedded and encapsulated in an appropriate medium. Various molds and plastic cassettes are used for encapsulation purposes. By using the microTec adapter system, the specimen can be aligned to the plane of cut.

6.2 Commencing the Cutting Operation Macro positioning of the specimen in relation to the knife cutter is accomplished by moving the base of the knife holder, or by operating the handwheel of the coarse feed drive (Fig. 2.11). With continued operation, cutting of the specimen commences up to the required depth of slice. The residues of the cut specimen so produced must be wiped away from the knife in an upwards direction from underneath using a brush.

Important!

Do not touch the knife with your fingers!

6.3 Instructions for Producing Sliced Microtomic Sections of the Specimen The following aspects are to be heeded in order to produce effective sliced microtomic sections of the specimen:

• always use a sharp knife,

• as wear occurs at the point of cut, slide the knife to the left or to the right, in order to be able to continue working with a sharp section of the knife,

• always keep the front and rear face of the knife clean. It is important to thoroughly remove any traces of paraffin wax.

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microTec


6.4 Cutting Action The cutting action of the rotary microtome is generated by rotating the handwheel (Fig. 1.1) in a clockwise direction. The cut is accomplished on the down stroke and retraction on the upwards directed stroke by further rotation of the handwheel (Fig. 1.1) in a clockwise direction.

6.5 Handwheel (Fig. 1)

The hand wheel (1) is integrated in the casing of the microtome (Fig. 1). The position of the handle (2) corresponds to the vertical position of the specimen. The handwheel locking device is situated on the handwheel (1). Releasing the handwheel locking device Press down the knob (refer to illustration) Locking the handwheel (1) Press the handwheel locking device (refer to illustration). Handwheel (1) engages in one of the 24 locking positions.

Important! For personal safety, the handwheel should always be locked in the upper region of vertical travel when exchanging specimens.

6.6 Coarse Advanced Drive To rapidly alter the distance between the knife and the specimen, the rotary microtome is equipped with a coarse advancement drive (11). The specimen fixture can be moved forwards or backwards horizontally by the handwheel for the coarse advancement drive (11).

Button for releasing

Handwheel locking

Handwheel for cutting movement

Handwheel

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microTec


6.7 Friction Clutch on the Coarse Advanced Drive Mechanism On reaching the front and rear positions of the specimen feed, a friction clutch built into the coarse advanced drive prevents the blocking of this coarse advanced drive. As soon as an audible engagement is triggered by the coarse drive, the feeding operation of the specimen stops. Upon rotation of the handwheel of the coarse advanced drive (11) in the opposite direction, the feeding motion is restored. If the feeding motion is not restored by this procedure, the handwheel of the coarse advanced drive mechanism (11) must be pressed inwards against the casing. Only a short forward or backward movement is needed to ultimately activate the feeding motion.

Important! Avoid touching the specimen or the specimen holder containing the knife when operating the handwheel of the coarse advanced drive mechanism.

6.8 The Handwheel of the Coarse Advanced Drive Mechanism (11) The handwheel of the coarse advanced drive mechanism (11) is integrated in the casing (Fig. 2)

6.9 Collision Protection Device On the specimen feed system reaching its forward terminal position, a red mark is visible on the cylinder (12). From this mark there is a further distance of adjustment of max. 2500 microns available. A second red mark then becomes visible, and the cylinder must be rotated back by means of the handwheel of the coarse advanced drive (11), before further cuts can be made.

Important!

As soon as the remaining distance of adjustment of 2500 microns is exceeded, the preselected thickness of cut is disabled by the in-built collision protection device. Any automatic adjustment by rotating the handwheel is now inactivated.

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microTec


7. Setting Up and Changing the Setup

7.1 Specimen Fixture To hold the specimens during cutting, various adapters and clamping fixture systems are available, based on the microTec principle of modular construction. In conjunction with the specimen orientation device, it is possible to orientate and align the specimen to the plane of cut is simple manner. An adapter piece is inserted at the orientation point where operations do not require such orientation.

7.2 Standard Specimen Clamping Fixtures The standard specimen clamping fixture (Fig. 3) is used for paraffin wax or plastic molded blocks of square or rectangular form. When using, the specimen is placed against the top fixed jaw (Fig. 3 – item 1) and clamped by the clamping screw (Fig. 3 – item 2).

Important!

On the grounds of stability, the specimen should not be allowed to protrude too far beyond the clamping jaws.

Fig. 3

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microTec


7.3 Orientable Adapter The orientable adapter (Fig. 3 – item 3) can optionally be attached by screws to the various specimen clamping systems and be used only in combination with the specimen orientation device. Specimen clamping systems of orientable design are supplied ex works equipped with the orientable adapter. Example (Fig. 3) The orientable adapter (Fig. 3 – item 3) is shown in this illustration attached to the standard specimen clamping fixture by 4 screws (Fig. 3 – item 4) and can be removed if required. Dismantling the standard specimen clamping fixture: Loosen 4 screws (Fig. 3 – item 4)

7.4 Fixed Adapter This adapter (Fig. 4 – item 1) is used as a fixed attachment directly on to the cylinder (Fig. 4 – item 2), and can be optionally fitted to various specimen clamping systems (Fig. 4).

Fig. 4

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microTec


7.5 Cylindrical Specimen Insert and Prism To clamp cylindrical specimens (Fig. 5), the cylindrical specimen insert (Fig. 5 – item 1), or the prism (Fig. 5 – item 2), can be inserted into the standard specimen clamping fixture (Fig. 5 – item 3), having the diameters - Ø 6 mm - Ø 15 mm - Ø 25 mm - Ø special diameters on request The location pin (Fig. 5 – item 4) engages the movable clamping jaw. Springs (Fig. 5 – item 5) on the cylindrical specimen insert (Fig. 5 – item 1) enable easy removal of the cylindrical specimens from the inserts.

Fig. 5

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microTec


7.6 Film (Thin Material) Clamping Fixture The film clamping fixture (Fig. 6) is a clamping system for film and foil materials or flat and thin specimens. To insert the specimen, the three threaded pins (Fig. 6 – item 4) are loosened, and the movable jaw (Fig. 6 – item 2) against the two compression springs, the setting of which is made by the two threaded pins (Fig. 6 – item 3), moved laterally. The specimen is held by the force of the springs, but can be repositioned. By screwing in the threaded pins (Fig. 6 – item 4), the specimen is securely clamped between the jaws of the film clamping fixture.

Fig. 6

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microTec


7.7 Universal Cassette Clamping Fixture The universal cassette clamping fixture (Fig. 7) is a quick change specimen system and is used for the clamping of cassettes. Inserting and removing the cassette Pull clamping lever (Fig. 7 – item 1) in the direction of the arrow (Fig. 7)

Important! Optimal clamping is only achieved if the bearing faces are clean of any paraffin wax residues.

Fig. 7

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microTec


7.8 Specimen Orientation Device Specimen clamping systems are attached to the orientation device (Fig. 8) by means of the orientable adapter (Fig. 8 – item 3). This way, orientation of the specimen in relation to the plane of cut is accomplished. Rotate the eccentric cam lever (Fig. 8 – item 1) towards the front. Specimen clamping fixture is released. Range of specimen clamping: 360º (Z-axis). Turn orientation screws (Fig. 8 – item 2) It is possible to orientate the specimen 8º in each direction (X-axis, Y-axis). The spring loaded pin (Fig. 8 – item 3) acts upon the orientation screws (Fig. 8 – item 2). After orientating the specimen, press the eccentric cam lever (Fig. 8 – item 1) upwards into the clamping position. Specimen is clamped. Cutting of the specimen can now commence.

Important!

The spring loaded pin (Fig. 8 – item 3) must remain fully screwed in, in order to keep the orientation screws loaded under spring pressure

Fig. 8

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microTec


7.9 Exchanging the Specimen Clamping Systems Exchanging the specimen clamping system Release (press downwards) the eccentric cam lever (Fig. 8 – item 1). Withdraw from the left out of the specimen orientation device (Fig. 8). Screw out the orientation screws (Fig. 8 – item 2) and spring loaded pin (Fig. 8 – item 3) a little way. Withdraw the specimen clamping fixture from the front from the specimen orientation device.

Important!

After exchanging the clamping system, make sure that the spring loaded pin (Fig. 8 – item 3) is screwed in again, otherwise there will be insufficient spring pressure imparted. Note In order to increase leverage at the handle of the eccentric cam lever (Fig. 8 – item 1), the handle sleeve (Fig. 8 – item 4) can be pulled out. After releasing, the handle sleeve springs back into its nominal position.

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microTec


8. Setting up the Knife

8.1 Knife holder The knife holders are user friendly designed and easy to clean due to its special surface treatment. The risk of injury during the setting up of the knife and the specimen is minimized by a laterally movable finger guard.

8.2 Knife holder The knife holder base is designed for universal use, and can be used for the “R“ type knife holder and also for the holder for throw-away blades. Mounting the base of the knife holder (Figs. 9 + 10). Fig. 2 Release clamping lever (Fig. 2 – item 7) by rotating to the left. Push the base of the knife holder on the guide bars (Fig. 2 – item 5). Push the base of the knife holder centrally over the clamping mechanism (Fig. 2 – item 5). Take note of the prism guide. When in the required position, rotate clamping lever (Fig. 2 – item 4) to the right. Allow the cutting edge of the knife to align flush with edge1. The height is adjusted by the knurled screw (Fig. 9 – item 7). Clamping is accomplished by means of the clamping screws (Fig. 9 – item 4).

Fig. 9

“R“ type knife holder

Base of knife holder

angle marking

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microTec


8.3 The “R“ type knife holder Assembling the “R“ type knife holder on the base of the knife holder Release clamping lever (Fig. 9 – item 1) in opposite direction to that of the arrow. Introduce the “R“ type knife holder into the clamping mechanism (Fig. 9 – item 2). Clamp at the required clearance angle position (Fig. 9 – item 5). Inserting the knife Slightly unscrew clamping screws (Fig. 9 – item 4). Push the knife in from the side. or Hinge up knife guard (Fig. 9 – item 3) in direction of arrow. Insert knife from above. Clamp knife by means of the clamping screws (Fig. 9 – item 4). Push finger guard (Fig. 9 – item 6) to center each time when Carrying out setting-up operation. Clamping levers are fitted to both sides of the knife holders to facilitate easier operation. Knife height adjustment device Loosen clamping screws (Fig. 9 – item 4). Adjustment to the height of the knife (1) is accomplished by screwing the knurled screws (Fig. 9 – item 7) to the left or the right. Tighten the clamping screws (Fig. 9 – item 4).

Fig. 9

“R“ type knife holder

Base of knife holder

angle marking

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microTec


8.4 The “RS” type knife holder

Stable version for Tungsten carbide conventional

steel knives with the central clamping plate providing

optimal stability. The knife is stabilized exactly,

where the highest cutting forces are applied. Full use

of the knife length.

Assembling the „RS“ type Knife holder on the base of the Knife Holder Release clamping lever in opposite direction to that of the arrow. Introduce the „RS“ type knife holder into the clamping mechanism. Clamp at the required clearance angle position. Inserting the blade Slightly unscrew clamping screws. Lift the valve completely bent up. Insert the blade and adjust with the knife height adjustment device the blade-edge to the white strip. Fit the valve and clamp it evenly with the two clamp screws. Open the blade guard Move the movable protection for opening outwards. Finger-blade guard when setting always closed hold.

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microTec


8.5 Holder for Throw-away blades Assembling the holder for throw-away blades on the base for the knife holder. Release clamping lever (Fig. 9 – item 1) in opposite direction to that of the arrow. Introduce the holder of the throw-away blades into the clamping mechanism (Fig. 9 – item 5). Clamp at the required clearance angle position (Fig. 9 – item 5). Inserting the throw-away blades Release clamping lever (Fig. 10 – item 3). Insert throw-away blades from the side in the gap between the bars and the clamping plate (Fig. 10 – item 4). Push throw-away blades to the center of the throw-away blade holder. Tighten clamping lever (Fig. 10 – item 3). Suitable Knife blades

Blades type Manufacturer Dimensions

ACCU-EDGE FEATHER 0,25 x 8,0 ACCU-EDGE FEATHER 0,32 x 14,1 Inscription of clamping plate

MS24, MS33, MS100, MS200, MS300 MICROS Austria 0,25 x 8,0 CUT 2511A Low Profile I

EDGE-RITE Thermo Scientific 0,30 x 8,0 CUT2511AE Low Profile I Plasma High Profile MICROS Austria 0,32 x 14,0 CUT 2511B High Profile I

Fig. 9+10

Holder for throw-away blades

Space for inscription of Clamping plate Fig. 9

Base for Knife holder

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microTec


Utilization of the blades In order to utilize the entire cutting edge of the blades. These blades can be pushed to the right or to the left. Release clamping lever (Fig. 10 – item 3). Push blade with the clamping plate to the left or the right. Tighten clamping lever (Fig. 10 – item 3).

8.6 Clearance Angle Adjustment The clearance angle between the knife cutting edge and the specimen can be adjusted as required to conform to the respective cutting requirements. The clearance angle setting is made by means of the clamping lever (Fig. 9 tem 1) at the base of the knife holder on all knife holder systems. Clearance angle setting Release clamping lever (Fig. 9 – item 1). Pivot the knife holder and /or the holder of the throw-away blades into the clearance position. The clearance angle can be read off from scale markings on both sides as an actual value. Tighten the clamping lever (Fig. 9 – item 1). Empirically, suitable microtomic sections are produced at a clearance angle of 15º. This clearance angle position is characterized by a longer mark on the knife holder base.

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microTec


9. Additional Functions

9.1 Additional Extras on Model CUT 4060 Specimen retraction on upward stroke The specimen retraction device instigates mechanical retraction of the cylinder during upward motion. Retraction by approx. 80 microns gives added care to protecting and extending the life of the knife and the specimen. Cutting counter with 6-digit. LCD display By pressing the RESET knob (10), the cutting counter is set to zero. After each complete cutting stroke the cutting counter increases the total of cuts by one. The battery of the cutting counter has a life of approx. 5 years. Only allow a service engineer to replace the battery.

LCD display Trimming system – adjustable to three setting levels Trimming level 1: 10 microns Trimming level 1: 20 microns Trimming level 1: 30 microns

10. Emergency Service, Customer Service microTec Laborgeräte GmbH Rudolf-Diesel-Str. 47 69190 Walldorf, Germany Phone: + 49 6227 8222-0 Fax: + 49 6227 8222-30 [email protected] www.micro-tec.de

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microTec


1st Step Remove all side and top sitting screws sides and take the complete cover of the transportation crate. 2nd Step Remove all hexagon headed screws around and the packaging frame. 3nd Step With both hands lift the instrument out from the transportation crate.

Important!

The unit is heavy – do not lift or carry the instrument by means of the handwheel.

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microTec


Notice

Rotary Microtome CUT 4060

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Transcript of Rotary Microtome CUT 4060