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Ronozyme p5000(Ct)
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Transcript of Ronozyme p5000(Ct)
The story of an excellent productcreated in a strong Alliance
told by: Yu-Guo Sun (DSM) & Frank Hatzack (Novozymes)
Frank Hatzack – China visit 2005
FU- 2
World leader in Enzymes!• R&D• Production• Granulation
World leader inAnimal Nutrition!• Nutrition Expertise• Global Distribution• Global Marketing
A strong alliance of two world leaderscreated an excellent product
Frank Hatzack – China visit 2005
FU- 3
Why is so successful?
An excellent Phytase enzyme...
…in an excellentgranulate...
…with excellent bio-efficacy!
Because it is:
Frank Hatzack – China visit 2005
FU- 5
Peniophora Phytase is a 6-Phytase
1
2
34
5 6
OPO32-
2- O3PO
2- O3PO2- O3PO
OPO32-
OPO32-
Aspergillusphytases
Peniophora phytase
Lily pollen phytase
Frank Hatzack – China visit 2005
FU- 6
Peniophora Phytase is a FAST Enzyme!
Peniophore lycii, wt
0
1
2
3
4
5
6
0 5 10 15
NatuPhos
0
1
2
3
4
5
6
0 5 10 15
NMR experiment measuring the number of P-groups released per minute核磁共振试验显示每分钟释放出的磷的集团…
Aspergillus phytase
min min
P-gr
oups
rele
ased 20 FYT/ml
1 FYT/ml
20 FYT/ml
1 FYT/ml
Peniophora phytase
Frank Hatzack – China visit 2005
FU- 7
Phytase pH profiles
0.0
0.3
0.5
0.8
1.0
1.3
3 4 5 6 7 8
pH
Rel
ativ
e ac
tivity
P.lycii
A.f icuum
Chineese
Solubility of phyate is zero above pH 6because of Ca-phytate precipitationpH>6时, 植酸的溶解性为零因为钙与植酸结合成植酸钙Ca-phytate,形成一种不溶性的沉淀。
The pH profile of RONOZYME P phytase植酸酶的PH值
more acidicpH optimum
than Aspergillusphytase!
FU- 8
Phytate degradation in the pig植酸在猪消化道中的降解
pH 1.5 – 5.45 min – 18 hours
pH 6.5 – 7.53 – 8 hours
pH 6.5 – 7.02 – 24 hours
Phytate solubility highpH OK for phytaselong retention time •Phytate solubility low
•pH not optimal for phytase
Frank Hatzack – China visit 2005
FU- 9
The digestive tract of poultry植酸酶在家禽消化道中的降解
pH 4 – 6.5up to 2 h
pH 3.5 – 4.50.5 min – 2 min
pH 2.5 – 3.51 min – 10 min
pH 5.5 – 7.51 – 4 h
Phytase pH optimumPhytate solubility high(if pH < 6) long retention timePhytate solubility high
OK for phytase•but short retention time
•Not optimal for phytase•Phytate solubility low
pH 6.5 – 7.02 – 6 h
前胃
肌胃
胰腺
泄殖腔
嗉囊
Frank Hatzack – China visit 2005
FU- 10
So: Peniophora phytase in is
1. is a very fast enzyme, faster than Aspergillus
2. with a more acidic pH optimum than Aspergillus
3. these features make it highly effective in the upper part of the digestive tract
Frank Hatzack – China visit 2005
FU- 12
Why do you need a stable phytase?
Want no losses during processingExcellent overall handling characteristics
No Dust & Physical strength
Want storage stability for long time
Storage stability even more importantEnzyme must be stable in presence ofminerals and other additives
Mash, Condensed
Premix
Want to use for pelleting at 85C Want storage stability
Pellet feed
Frank Hatzack – China visit 2005
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CT – Coated Thermostable Granulate
Multi-layer coating1. for pelleting stability2. enzyme release3. dust-free
Enzyme stabilized inmineral&carbohydratematrixfor enzyme stability
Cellulose fibers for physical stability
400-800 microns
Patented technology
Small, defined particles1. high particle number per gram2. Excellent mixability3. Excellent homogeneity in feed
Frank Hatzack – China visit 2005
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CT Coating has dual function
1. To protect the enzyme during pelleting
2. To allow release of enzyme activity in stomach
Frank Hatzack – China visit 2005
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0
50
100
150
200
RonozymeP5000 CT
Phytase N Phytase P1 Phytase P2 Phytase C2 Phytase B
mg
is dust-free (< 1 mg)
Frank Hatzack – China visit 2005
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Why is dust-free important?
1. Dust means losses of product during handling
2. Dusty products do not mix well
3. Dust is a potential health hazard
Frank Hatzack – China visit 2005
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See the point of coating?
Spray onorganic carrier
Competitor
CT Granulates
Enzyme totallyexposed!
-> low stability!
Frank Hatzack – China visit 2005
FU- 21
Dust measurement PhytaseDone with a Heubach Dust Meter
植酸酶粉尘检测的结果RONOZYME P
Phytase P1 and F
Phytase N5000 G
Frank Hatzack – China visit 2005
FU- 23
What is the relation between particle size, particlenumber and mixability?
What does this mean for the animal??
Frank Hatzack – China visit 2005
FU- 24
Small granulate size meanshigh particle number per gram
• particle size of 400 – 800 µm
• Average particle number per gram is > 14000!
0.0
5.0
10.0
15.0
20.0
25.0
30.0
35.0
<180 180-250 250-300 300-355 355-425 425-500 500-600 600-710 710-850 > 850
particle size intervals [祄 ]
% o
f par
ticle
s
Frank Hatzack – China visit 2005
FU- 25
• Phytase N has 5000 Granules/g. Dose is 100 g/ton feed:
500.000 Granules/ton feed-> 500 Granules/kg feed -> 10 Granules/20g feed
• RONOZYME P 5000 CT has 14.200 Granules /g, dose is 100 g/ton feed
1.420.000 Granules/ton feed -> 1.420 Granules/kg feed -> 28 Granules/20g feed
With RONOZYME P there is a 3 times higher probability that a
broiler chicken gets the amount of phytase it should get each day!
What does number of particles per gram meanfor mixability and for the starter chick?每克产品中的颗粒数对小鸡料意味着什么?
Frank Hatzack – China visit 2005
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So, if competitors dose at only 50 g per ton…如果有些产品每吨只有50克添加量…
…and their product is a powder-form phytase with heterogeneous particle size distribution…
…how do you think the broiler chicken is feeling?
will I get my phytase granules
today?
Frank Hatzack – China visit 2005
FU- 27
0
20
40
60
80
100
120
Samples
Enz
yme
cont
ent i
n fe
ed (%
of
aver
age)
Excellent mixability in a broiler premix
肉鸡预混料中卓越的混合均匀度
Coefficient of variation = 6.9%
变异系数 = 6.9%
VFP 21033B
Frank Hatzack – China visit 2005
FU- 28
0
20
40
60
80
100
120
140
Samples
Enz
yme
cont
ent i
n fe
ed (%
of
aver
age)
Excellent mixability in a broiler feed
肉鸡饲料中的优异混合均匀度
Coefficient of variation = 9.2%
变异系数 = 9.2%
VFP 21033A
Frank Hatzack – China visit 2005
FU- 29
0
20
40
60
80
100
120
Samples
Enz
yme
cont
ent i
n pr
emix
(% o
f ave
rage
Excellent mixability in a swine premix
猪的预混料中有非常好的混合均匀度
Coefficient of variation = 5.9%
变异系数 = 5.9%
VFP 21033BFrank Hatzack – China visit 2005
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Luna No.: 2004-17531-01 / TKv, PEJ•3 different batches of each product•tested independently in 2-3 runs
60
65
70
75
80
85
90
95
100
RONOZYME P RONOZYME P5000
Rec
over
y [%
] 85%
CV: 8.5% CV: 3.3%
88%
Excellent pelleting stability even at 95ºC95ºC制粒时的卓越稳定性
conditioning time 30 sec
Frank Hatzack – China visit 2005
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93 89
43
15 13
95
0
20
40
60
80
100
70癈 at 80 癈 at 90 癈
Ret
entio
n, (%
mas
h)
Ronozyme P5000 K24014
trial VFP24026
Has a much higher pelleting stability than local competitor与竞争对手在制粒稳定性上的比较
Frank Hatzack – China visit 2005
conditioning time 30 sec
FU- 33
Has better storage stability in pelleted feedthan other phytase products
85
67
39
81
56
21
84
50
33
77
47
21
0
20
40
60
80
100
RONOZYME P 5000(CT)
Phytase N Phytase FRec
over
y (%
of a
ctiv
ity in
initi
al p
elle
ted
feed
)
Initial pellets 1 month @ R.T. 1 month @ 35 deg. C 3 months @ R.T.
VFP 21052
30 secs conditioning
Frank Hatzack – China visit 2005
FU- 34
Comparative pelleting trial from Denmark Kolding City Biotech Institute, 2005
0.0
20.0
40.0
60.0
80.0
100.0
120.0
75C 75C 85C 85C 90C 90C 95C 95C
P CT P 5000 CT Competitor 1 Competitor 2
30 sec conditioning
75C 75C 85C 85C 90C 90C 95C 95C
RONOZYME P5000RONOZYME P (old product)
Competitor 1Competitor 2
Frank Hatzack – China visit 2005
FU- 37
1796
2107 2110
1600
1700
1800
1900
2000
2100
2200
Control RONOZYME P (CT) RONOZYME P5000(CT)
Wei
ght g
ain
(g/b
ird)
Biological efficacy in broilersGrowth Performance (d. 1 – 36)
肉鸡的生物学活性 生长成绩(d. 1 – 36 )
broiler trial (ME08/02)
Mean values in a row with different superscripts were significantly different: a-b (P < 0.05)
Frank Hatzack – China visit 2005
FU- 38
1.6
1.5891.583
1.55
1.56
1.57
1.58
1.59
1.6
1.61
1.62
Control RONOZYME P (CT) RONOZYME P5000(CT)
FCR
(g fe
ed /
g ga
in)
Biological efficacy in broilersFCR (d. 1 – 36)
肉鸡的生物学活性 饲料转化率( d. 1 – 36 )
broiler trial (ME08/02)
Mean values in a row with different superscripts were significantly different: a-b (P < 0.05)
Frank Hatzack – China visit 2005
FU- 39
49.2
52.5 52.3
47
49
51
53
Control RONOZYME P (CT) RONOZYME P5000(CT)
Bon
e A
sh (%
)Biological efficacy in broilers
Bone Ash ( % )肉鸡的生物学活性 骨骼灰份( % )
broiler trial (ME08/02)
Frank Hatzack – China visit 2005
FU- 40
22.9
37.1
41.7
20
25
30
35
40
45
Control RONOZYME P (CT) RONOZYME P5000(CT)
Pho
spho
rus
dige
stib
ility
(% o
f int
ake)
Biological efficacy in pigsPhosphorus digestibility ( % )
猪的生物学活性 磷的消化率( % )
pig trial (1008644)
Frank Hatzack – China visit 2005
FU- 41
49.5
56.5
64.1
45
50
55
60
65
Control RONOZYME P (CT) RONOZYME P5000(CT)
Cal
cium
dig
estib
ility
(% o
f int
ake)
Biological efficacy in pigsCalcium digestibility ( % )
猪的生物学活性 钙的消化率
pig trial (1008644)
Frank Hatzack – China visit 2005
FU- 42
720
794789
700
720
740
760
780
800
Control RONOZYME P (CT) RONOZYME P5000(CT)
Dai
ly m
ean
gain
(g/d
ay)
Biological efficacy in pigsGrowth performance ( d. 0 – 30) g/day
猪的生物学活性 生长成绩( d. 0 – 30) g/day
pig trial (1008644)
Frank Hatzack – China visit 2005
FU- 43
2.91
2.74
2.64
2.6
2.7
2.8
2.9
3
Control RONOZYME P (CT) RONOZYME P5000(CT)
FCR
(Kg
/ Kg)
Biological efficacy in pigsFCR (d. 0 – 30 ) kg/kg
猪的生物学活性 饲料转化率 (d. 0 – 30 ) kg/kg
pig trial (1008644)
Frank Hatzack – China visit 2005
FU- 44
202
236
263
160
200
240
280
Control RONOZYME P (CT) RONOZYME P5000(CT)
Bre
akin
g st
reng
th (N
)
Biological efficacy in pigsBone strength
(external metacarpal + metatarsal combined)猪的生物学活性 骨骼强度 (外掌骨+)
pig trial (1008644)
Frank Hatzack – China visit 2005
FU- 45
Growing pigs:Ileal apparent digestibility improvements
生长猪回肠表观消化率的改善
Val
Tyr
Thr
+ 2.6%
+ 1.8%
+ 2.1%
+ 6.2%
+ 4.3%
+ 2.7%
+ 1.4%
Ser
Pro
Phe
Orn
Met
Lys
Leu
+ 3.5 %
+ 4.7%
+ 5.7%
+2.3%
+ 32%
+ 2.1%
+ 8.4%
+ 1.9%
+ 1.8%
+ 2.9%
Ile
Hyp
His
Gly
Glu
Cys
Asp
+ 2.2%
+ 4.4%
+ 3.7%
+ 1.3%
+ 30.6%
+ 41.4%
+ 38.4%
Arg
Ala
Total N
Energy
Ca
total IPP
total P*
*measured as total digestibility
RONOZYME P (CT) at 750 FYT/kg. Eight pigs at 60 days (WhitexLandracexPietrain). Ileal-rectal anastomosis. Maize/SBM/barley diet fed ad libitum. 0.47 % P in diet. 5 periods of one week, 3 adaptation days, 4 ileal content sampling days. S06-2001.
Frank Hatzack – China visit 2005