Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,
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Transcript of Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,
Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa)
Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,Gu¨nter Theißen,* and Zheng Meng*
報告者 :劉育羣 N99H0011專討老師 :鄭伯智 林宏榮 醫師
日期 :05/08/2011
Oryza sativa 水稻 Sorghum bicolor 蜀黍
Hordeum vulgare 大麥 Triticum aestivum 小麥 Zea maysZea mays 玉玉米米
糧食作物中包括許多禾本科植物糧食作物中包括許多禾本科植物
• 中文名稱:水稻• 英文名稱: Rice• 學 名: Oryza sativa L.• 科名:禾本科 (Gramineae)• 別 名:稻仔• 原產地:中國長江流域。 1973 年在浙江省餘姚縣首次發
現約 7000 年前長江下游地區河姆渡文化遺址中有大量的稻穀遺存,證明當時已有農業生產,已開始種植水稻。
• 分布:台灣全島中低海拔水源充足,灌溉方便之平原。• 用途: 稻米以碾成白米供食用為主,是人類主食,次可
釀酒,並可製造米粉、米麩、蘿蔔糕等。• 特 性:水稻是台灣最重要的農作物,也是亞洲人民食用
的主食。水稻屬於禾本科單子葉的草本植物,喜歡溫暖多雨的氣候,台灣水稻一年可以栽種兩次,栽植水稻要先育秧苗,然後再移植到水田裏,約3至4個月可以收穫。我們吃的白米就是稻子的胚乳。
Oryza sativa 水稻
小穗由一朵小花組成,外由外穎及內穎包被,單生於穗柄上。
小穗→
OutlineOutline
•Introduction•Experimental Procedures•Results•Conclusions
Introduction
• The MADS-box family transcription factors play key roles in controlling development and signal transduction in eukaryotes.
• Phylogenetic analyses split the MADS-box gene family into two classes, termed type I (SRF-like) and type II (MEF2-like) genes.
MADS-box Gene FamilyMADS-box Gene Family
Messenguy and Dubois (2003)
Alvarez-Buylla et al. (2000)
de Folter S and Angenent GC (2006)
Soltis DE et al., (2007)
New Floral ABCE and Quartet Model
Se – sepal ( 花萼 )
Pe – petal ( 花瓣 )
St – stamen ( 雄蕊 )
Ca – carpel ( 心皮 )
Evolutionary implications of class Evolutionary implications of class E E genesgenes in rice in rice
The class E gene function specifying the identity of lodicules, stamens and carpels may require the direct interaction of OsMADS1 and OsMADS7/8 during spikeletmeristem development.
lemma (外穎 ) , stamen (雄蕊 ) , carpel (心皮 ) , palea (內穎 ) , lodicule (漿片 ) , sterile lemma (護穎 ), pedicel(花梗 )
• Rice transformation• Scanning electron microscopy• In situ hybridization• RT-PCR and real-time PCR expression analysis• Yeast two-hybrid (Y2H) analyses• Coimmunoprecipitation (Co-IP) analyses of the ric
e SEP-like proteins in Arabidopsis mesophyll protoplasts
Experimental Procedures
Results
Molecular characterization of RNAi transgenic plants for rice SEP-ike genes
Phenotypes of wild-type and OsM7/8-RNAi spikelets
WT WTOsM7/8 -RNAi
OsM7/8-RNAi
OsM7/8 -RNAi
Scanning electron microscopy of epidermal cells expression patterns detailed structure
OsM7/8-RNAi
WT
OsM7/8 -RNAi
藉由這樣的實驗作者是要看小穗在 OsM7/8-RNAi 和 WT 當中,表皮細胞細部構造的
表現情形
Phenotypes of the OsM1/5/7/8-RNAi inflorescences and spikelets
WTOsM1/5/7/8-RNAi OsM1/5/7/8-RNAi
6 9
OsM1/5/7/8-RNAi
Under the Scanning electron microscopy the different developmental stages, formation
of inflorescence meristem changes
藉由這樣的實驗作者是要看小穗在 OsM1/5/7/8-RNAi 和在花序中產生變化所表現的
情形
Characteristics of the florets in transgenic lines
lodicule (漿片 )
stamen (雄蕊 ) carpel (心皮 )palea (內穎 )
lemma (外穎 )
此表格作者所要呈現的是轉殖株在花部位的特性
Expression patterns of OsMADS7 and OsMADS8 during inflorescence and spikelet development as revealed by in situ
hybridization
OsMADS7
OsMADS8 OsMADS7 OsMADS8
pbm代表主要分支
sbm代表次要分支smc代表分生組織細胞 ;tsn代表末端分生組織 ;lsm代
表橫向分生組織
OsMADS7
OsMADS8
OsMADS7 OsMADS8
Meristem at different times for each organ, in part on the show are different
藉由這樣的實驗作者是要看 OsMADS7.OsMADS8 在花序與小穗中分生組織表現的情形
fm代表分生組織
Yeast two-hybrid assays. Interaction patterns of the rice SEP-like proteins including OsMADS1, OsMADS5, OsMADS7 and
OsMADS8 are shown
藉由這樣的實驗作者證實了 OsMADS1.OsMADS7.OsMADS8 蛋白彼此之間的結合
Interaction analyses of four rice class E proteins in vivo
藉由這樣的實驗作者證實了水稻中 E class 蛋白彼此之間的結合能力
conclusion
• Silencing of both OsMADS7 and OsMADS8 causes severe morphological alterations of floral organs.
• Simultaneous silencing of OsMADS1, OsMADS5, OsMADS7 and OsMADS8 transforms all floral organs into leaf-like structures.
• OsMADS1, OsMADS7 and OsMADS8 can cross-interact to each other while OsMADS5 can not.
Thanks for your listening