©2016 Waters Corporation. Waters, The Science of What’s Possible, ACQUITY, ACQUITY UPLC, Atlantis, CORTECS, and XSelect are registered trademarks of Waters Corporation. ACQUITY Arc is a trademark of Waters Corporation. November 2016 720005828EN LM-PDF

min0 1 2 3 4 5 6 7

1. Propyl gallate 2. 2,4,5-Trihydroxybutyrophenone 3. tert-Butylhydroquinone 4. Butylated Hydroxyanisole 5. 2,6 di-tert-Butyl-4hydroxymethylphenol 6. Octyl gallate

1 2

3

4

5

6

Atlantis T33.0 x 75 mm, 3 µm Peak capacity = 100

XSelect HSS T3 XP 3.0 x 75 mm, 2.5 µm Peak capacity = 114

CORTECS T3 3.0 x 75 mm, 2.7 µm Peak capacity = 129

System: ACQUITY® Arc™ with 2998 PDA Mobile phase A: Water Mobile phase B: Acetonitrile Mobile phase C: Methanol Mobile phase D: 2% Formic acid in water Flow rate: 0.85 mL/min Injection volume: 2.1 µL Column temperature: 30 °C

Gradient:

Time (min)

%A

%B

%C

%D

0.00 60 17.5 17.5 5

2.00 40 27.5 27.5 5

3.00 14 40 41 5

7.50 14 40 41 5

HOW TO IDENTIFY WHICH T3 COLUMN IS RIGHT FOR YOU

Is the ability to easily transfer methods to other platforms

most important?

Are faster run times and improved resolutions

most important?

Is isolating and purifyingpolar compounds most important?

[ T3 COLUMNS ]

Retaining Polar CompoundsT3 columns are ideal for polar compound retention

What is a T3 column?T3 reversed-phase columns are designed for separating polar compounds. An optimized particle pore size and C18 surface concentration enables reliable operation with 100% aqueous mobile phases needed for retaining highly polar analytes. Waters® offers a full range of CORTECS® Solid-Core T3, XSelect® HSS T3/ACQUITY UPLC® HSS T3, and Atlantis® Silica T3 columns and method validation kit options based on your separations needs.

www.waters.com/T3

DID YOU KNOW?

Fully-porous packing materials such as Atlantis T3 and HSS T3,

exhibit higher retention, while the solid-core packing

materials used in CORTECS T3 is designed for maximum

e�iciency and peak capacity.

Balanced retention for polar analytes

Universal polar compound retention

High mass loading and retention

4

AU

0.00

0.05

0.10

0.15

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00

12

3

4

5

6

AU

0.00

0.02

0.04

Minutes0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00USP Res 2,3 = 3.77

USP Res 5,6 = 3.68

©2016 Waters Corporation 10

AU

0.00

0.02

0.04

0.06

0.08

0.10

0.12

min0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00

CORTECS T3 2.7 m on ARC (Path 1)Chromatogram

1

2

3

4

5

6

AU

0.00

0.02

0.04

Minutes0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00

USP Res 2,3 = 4.94USP Res 5,6 = 4.40

The higher e�iciency of the CORTECS T3 column provides faster runtimes while

improving resolution.

Atlantis T3 preparative columns outperform conventional C18 columns

for polar compound loading.

Because ACQUITY HSS T3 and XSelect HSS T3 di�er only in

particle size, transfer between columns and instrument

platforms is straightforward.

0 2 4 6 8

AU

0.0

1.0

1.5

2.0

2.5

AU

0.0

1.0

1.5

2.0

2.5

0.5

0.5

Atlantis T3, OBD™ Prep 5 µm, 19 x 50 mm

Conventional C18

5 µm, 19 x 50 mm

1 2

3

V0 = 0.6 min

V0 = 0.5 min

1

2

3

Minutes

AU

0.00

0.10

0.20

0.30

0.40

0.50

min

min

0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 1.10

AU

0.00

0.10

0.20

0.30

0.40

0.50

0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00

ACQUITY HSS T3, 2.1 x 50 mm, 1.8 μm

XSelect HSS T3, 4.6 x 100, 3.5 μm

1. Acetaminophen2. Caffeine3. 2-Acetamidophenol4. Acetanilide5. Acetylsalicylic acid6. Phenacetin

1 4

5

6

23

Atlantis T3, 4.6 x 150 mm, 5 µm

CORTECS T3, 3.0 x 75 mm, 2.7 µm

faster analysis = more info in less time

stab

le

at high pressure30%lower backpressure

more e�icient

39%

easily transfer methods

1. Pyridoxine 2. Acetaminophen3. Caffeine

min

1. Propyl gallate2. 2,4,5-Trihydroxybutyrophenone3. Tert-butylhydroquinone4. Butylated hydroxyanisole5. 2,6-Di-tert-butyl-4-a6. Octyl gallate

2xmore

retention

even at low pH

great peaks