SUMIT KUMAR MISHRA

Mobile - +91-9481479628/ 7879518130 E-Mail: sumitmishra1089@gmail.com

OBJECTIVE

Looking forward to take up a challenging research in biology, to obtain a doctoral position, and to utilize my analytical and practical skills to contribute significantly towards human welfare and personal excellence.

PRESENT OCCUPATION

Working as a Research Scholar, in Dept. Of Animal Biology, Hyderabad Central University, since August 2015.

EDUCATIONAL QUALIFICATION

S.No NAME OF EXAMINATION

YEAR OF PASSING

BOARD/UNIVERSITY

SCHOOL/COLLEGE

PERCENTAGE

1. M.Sc 2012 RTMNU Hislop College 74.2%

2. B.Sc 2010 RDVV St. Aloysius 73.6%

3. SSCE 2007 M.P.Board GGSKS 76.0%

4. HSCE 2005 M.P.Board GGSKS 86.0%

Research Experience

1. Junior Research Fellow at Indian Institute of Science (IISc), Bangalore, India. (April 2014-June 2015) 2. Trainee at Indian Immunologicals Limited, (IIL) Hyderabad, India (November 2012-April 2013)3. Project trainee at All India Institute of Medical Science (AIIMS), New Delhi. (December 2011-May 2012)

Projects Handled

Project 1: “Establishment of Ectopic Artificial Human Liver In Mice”

Hepatitis C Virus (HCV) is a hepatotrophic positive sense, single-stranded RNA virus within Flaviviridiae family and its replication is restricted to humans and experimentally infected chimpanzees. The rate limiting step in understanding the pathophysiology of HCV-infection has been a paucity of suitable in vivo model. A versatile preclinical model of HCV- infection can assist in further understanding of virus biology. So, we were interested in developing a humanized mice with an ectopic artificial liver; a molecular mimic of human liver that can help us in better understanding of the viral dynamics within the host, so as to propose a credible in vivo model system for disease modeling.

Project 2: “Development of Anti - TNFα Monoclonal Antibody for Therapeutic Application”

TNFα is an inflammatory cytokine involved in systemic inflammation. TNF-α impede insulin transduction, and has an effect on metabolism of glucose. Perturbation in the TNF-α metabolism have also been implicated in metabolic disorders, such as obesity and insulin resistance, indicating that disturbance of TNF-α metabolism may affect the onset of type 2 diabetes mellitus and the progression of the disease. Attempts have been made to develop new TNF α blockers, but none is very effective for use as a therapeutic in case of diabetes. Since each monoclonal antibody is unique in itself because of unique V-D-J recombination that happens in B cells even though it see the same antigen. We generated Anti TNF α monoclonal antibodies in our laboratory. Initial screening for the affinity of panel of different monoclonal antibodies against TNF α protein gave a very good affinity, and the affinity of the antibodies generated in our laboratory is even higher than commercially available Anti TNF α monoclonal antibody (Adalimumab) as found from Competitive ELISA. Long term goal is to humanize the antibody for its use as a therapeutic.

Project 3: “Brucella Glycoconjugate Vaccine: Analysis and Characterization”

Glycoconjugate vaccines, containing purified bacterial lipopolysaccharides covalently attached to an appropriate carrier protein, represent the perfect tool to generate protective immune response against a broad spectrum of diseases, caused by pathogens. A wide range of analytical and physicochemical tools are available today to asses and evaluate the product and their raw-materials. The analysis and characterization represents a key role in vaccine development process from lab to clinic. In this report, we have evaluated the purity and characterization of brucella glycol-conjugate and its initial materials such as LPS and OMP. A quorum of physiochemical and spectroscopic methods were used to determine the purity, identity, molecular weight profiles and to quantify the final vaccine and its individual components, to ensure consistent lots of vaccine with same potency and efficacy.

M.Sc (Biotechnology) Dissertation Project

Title: “Oxidative Stress amongst Various Immune Cell Subsets during M.tuberculosis Infection”Institution: Dept. of Transplant Immunology & Immunogenetics (Dr. D K Mitra’s Lab) AIIMS, New Delhi.Duration: Dec’11-Apr’12 (5 months)

TRAINING

Title: “Brucella Glycoconjugate Vaccine: Analysis and Characterization”Institution: Indian Immunologicals Limited, Hyderabad.Duration: Nov’12-Apr’13 (6 months)

Title: “Basic and Advanced Instrumentation Techniques”Institution: Career College (Global Green Scientific Activity Cell), Bhopal.Duration: 4 days

TECHNICAL EXPERTISE

Analytical Techniques: Protein and lipopolysaccharides analysis by High Performance Liquid Chromatography (HPLC), Particle Size Analysis, Spectrophotometric Analysis, Dialysis, Protein &Carbohydrate Estimation.

Purification Techniques: Protein and lipopolysaccharides purification by affinity chromatography. Purification of his-tag protein using Ni- NTA column, Antibody purification using Protein-A coloumn,Virus like particle (VLP) production and purification through density gradient centrifugation.

Protein Work: SDS-PAGE, Native-PAGE, Western Blotting, Coomassie Staining, Silver Staining for Proteins & lipopolysaccharides.

Immunological Techniques: Monoclonal antibody production, FACS based assay, Cell surface markers, intracellular cytokines and FOXP3 staining of PBMNCs, Different variants of ELISA, Dot blot.

Cell Culture Techniques: Experience of handling and maintaining adherent (Hep-G2, Huh-7, HUVEC, NIH-3T3), semi-adherent (hybridoma cells) and suspension cell culture (Sf 21), Isolation of peripheral blood mononuclear cells (PBMNCs) from biological samples, Infection of cells with bacterial antigen.

Animal Handling (Mice): Proper handling of mice (Intraperitoneal & subcutaneous injection, tail bleeding, collecting heart blood), Intraperitoneal implantation of polymeric scaffold.

Microbial Techniques: Plasmid DNA isolation, Restriction digestion, Sub-cloning & expression of proteins in bacterial system.

SEMINARS AND WORKSHOPS

“Participated in NextGen, Genomics, Biology, Bioinformatics and Technologies (NGBT) 2015; International Conference on next generation sequencing.

“Biotalk 2011, a National Level Symposium on Anti-Immunology: Pathogens as Cheats” organized by Hislop School of Biotechnology, Nagpur.

“National Seminar on Biotechnology for the 21st Century: New Horizons”organized by St.Aloysius College, Jabalpur.

“National Seminar on Nanotechnology: A Futuristic Application in All Disciplines of Science” organized by St.Aloysius College, Jabalpur.

Edu-workshop on “High Melting Technology and Flowcytometry” in collaboration with Genetix, New Delhi &BD Biosciences, Mumbai.

ACCOLADES

Qualified GATE-2015. Received BITP (Biotech Industrial Training Programme) certificate sponsored

by Department of Biotechnology, Government of India. (2012-2013) 1st prize in poster competition in National Seminar on “Nanotechnology: A

Futuristic Application in All Disciplines of Science” in 2009. 2nd prize in Science Exhibition-2005 for “Static Model” Rewarded with “Excellence Award” for excellence in academics by STG

International Ltd, Jabalpur, in 2002. Successfully participated and won prize in various Zone and Block level Science

Exhibition.

PERSONAL DOSSIER

Date of birth: 11th Oct 1989.

Gender: Male

Marital Status: Single

Nationality: Indian

Linguistic Abilities: English, Hindi.

Address: H.No: 794 Frontier Building, Badi Omti, Jabalpur (M.P.) 482002

REFERENCES

Dr. Anajli A KarandeProfessorDepartment Of Biochemistry,Indian Institute of Science (IISc), Bangalore.India – 560012,Phone- +91-80-22932306,E mail – anjali@biochem.iisc.ernet.in

Dr. Burki RajendarAssistant General ManagerBiologicals E Ltd, Hyderabad,India,Phone - +918790888740E mail – burkirajendar@gmail.com

Dr. Avinash UpadhyayDirectorHislop School of Biotechnology, Nagpur,India,Phone - +919822573332E mail – avinashjee@gmail.com

DECLARATION

I hereby declare that all the above mentioned information’s are correct to the best of my knowledge and I bear the responsibility for the correctness of the above mentioned particulars.

Date: (SUMIT KUMAR MISHRA)