Resume Metode Ekstraksi mtDNA Vendi Eko Susilo...

Achmad Farajallah | Resume Metode Ekstraksi mtDNA Vendi Eko Susilo G352110071Copyright Achmad Farajallah [email protected]://achamad.staff.ipb.ac.id/2011/11/26/resume-metode-ekstraksi-mtdna/

Resume Metode Ekstraksi mtDNA Vendi EkoSusilo G352110071

Resume Metode Ekstraksi mtDNAoleh Vendi Eko Susilo G352110071

No Nama Hewan Sample Metode Genome Target1 Drosophila whole body?

Adultdrosophila

alkaline lysis mtDNA

2 collectedduringautopsy byDr. William(human)

Hair the silica/guanidinethiocyanate (GuSCN)

total genome

3 yeast species cells 1) boiling, 2) glass beaddisruption, and3) acommercially available kit(Gen Toru-Kun Kit/GenTLE)

total genome

4 Hornbill Feathers use a single extractionsstep with chelex as aextraction medium

total genome

5 Insect eggs QIAamp® columns(Vallencia, CA)

haploid genome, mtDNA

6 cow fecal RBB+C ?

7 bear blood Qiam kit total genome

page 1 / 76


8

page 2 / 76


chicken, goat,

page 3 / 76

Fecal QIAamp DNA stool total genome, include gut


cattle and pigs

page 4 / 76

purification kit bacterial consortium


9

page 5 / 76


Human

page 6 / 76


Human fecal

page 7 / 76


- UltraClean Fecal DNAextraction kit- ExtractMaster Fecal DNAextraction kit

page 8 / 76


total genome, include gutbacterial consortium

page 9 / 76


10

page 10 / 76


human

page 11 / 76


semen

page 12 / 76


FTA technology

page 13 / 76



page 14 / 76page 14 / 76


page 15 / 76


page 16 / 76


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Sumber:

1. Rapid Isolation Methodof Animal MitochondrialDNA by the AlkalineLysis Procedure. KoichiroTamura 1 and TadashiAotsuka 1': BiochemicalGenetics, Vol. 26, Nos.11/12, 1988

2. A Silica-BasedMitochondrial DNAExtraction MethodApplied to Forensic HairShafts and Teeth. Lori E.Baker,1 M.A.; William F.McCormick,1 M.S., M.D.;and Karla J. Matteson,2Ph.D. (2001)

3. Comparison of DifferentMethods for Extraction ofMitochondrial DNA fromHuman PathogenicYeasts. Yohko Yamada*,Koichi Makimura1,2,Hossain Mirhendi1,Kumiko Ueda1, YayoiNishiyama1,2, HideyoYamaguchi1,2 andMasako Osumi; Jpn. J.Infect. Dis., 55, 122-125,2002

4. DNA extraction,amplification and directsequensing from horbillfeathers. Philip A Morina,Jeane Messier, and DavidS woodroff; J.Sci.Soc.Thailand, 20 (1994)

page 19 / 76


31-345. Human and Insect

Mitochondrial DNAAnalysis fromMaggots. Jeffrey D.Wells, Ph.D.; FrancescoIntrona, Jr., M.D.;Giancarlo Di Vella, Ph.D.,M.D.; Carlo P.Campobasso, M.D.; JackHayes, Ph.D.; and FelixA. H. Sperling, Ph.D ;JOURNAL OF FORENSICSCIENCES (2001).

6. Improved extraction ofPCR-quality communityDNA from digesta andfecalsamples. Zhongtang Yuand Mark Morrison;BioTechniques36:808-812 (May 2004)

7. Techniques forapplication of faecal DNAmethods to field studiesof Ursids. S. K. WASSER,C. S. HOUSTON, G. M.KOEHLER,* G. G. CADDand S. R. FAIN; MolecularEcology 1997, 6,1091–1097

8. A rapid and low-costDNA extraction methodfor isolating Escherichiacoli DNA from animalstools. Barnard, T. G.1*,Robertson, C. A.1, Jagals,P.1 and Potgieter, N.2;African Journal ofBiotechnology Vol. 10(8),pp. 1485-1490, 21February, 2011

9. Comparison of methodsfor the extraction ofbacterial DNA fromhuman faecal samples

page 20 / 76


for analysis by real-timePCR. E.A Nelson1, 2, E.APalombo1, and S.RKnowles2; (2010).

10. 10. Application of FTAtechnology to extractionof sperm DNA frommixed body fluidscontainingsemen. Yoshihiko Fujita,Shin-ichi Kubo; Y. Fujita,S.-i. Kubo / LegalMedicine 8 (2006) 43–47

Resume MetodeEkstraksi mtDNAoleh Vendi Eko Susilo G352110071

page 21 / 76



Adultdrosophila


2 collectedduringautopsy byDr. William(human)


total genome

3 yeast species cells 1) boiling, 2) glass beaddisruption, and3) acommercially available kit(Gen Toru-Kun Kit/GenTLE)

total genome

4 Hornbill Feathers use a single extractionsstep with chelex as aextraction medium

total genome

5 Insect eggs QIAamp® columns(Vallencia, CA)


6 cow fecal RBB+C ?

7 bear blood Qiam kit total genome

8 chicken, goat,cattle and pigs

Fecal QIAamp DNA stoolpurification kit


9 Human Human fecal - UltraClean Fecal DNAextraction kit- ExtractMaster Fecal DNAextraction kit


10 human semen FTA technology haploid genome, mtDNA

page 22 / 76


Sumber:




4. DNA extraction,amplification and directsequensing from horbillfeathers. Philip A Morina,Jeane Messier, and DavidS woodroff; J.Sci.Soc.Thailand, 20 (1994)31-34

5. Human and InsectMitochondrial DNAAnalysis fromMaggots. Jeffrey D.Wells, Ph.D.; Francesco

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Introna, Jr., M.D.;Giancarlo Di Vella, Ph.D.,M.D.; Carlo P.Campobasso, M.D.; JackHayes, Ph.D.; and FelixA. H. Sperling, Ph.D ;JOURNAL OF FORENSICSCIENCES (2001).




9. Comparison of methodsfor the extraction ofbacterial DNA fromhuman faecal samplesfor analysis by real-timePCR. E.A Nelson1, 2, E.APalombo1, and S.RKnowles2; (2010).

10. 10. Application of FTAtechnology to extraction

page 24 / 76


of sperm DNA frommixed body fluidscontainingsemen. Yoshihiko Fujita,Shin-ichi Kubo; Y. Fujita,S.-i. Kubo / LegalMedicine 8 (2006) 43–47

Resume MetodeEkstraksi mtDNAoleh Vendi Eko Susilo G352110071


Adultdrosophila


page 25 / 76


2

page 26 / 76


collectedduringautopsy by

page 27 / 76


total genome


Dr. William(human)

page 28 / 76


3

page 29 / 76


yeast species

page 30 / 76


cells

page 31 / 76


1) boiling, 2) glass beaddisruption, and3) acommercially available kit(Gen Toru-Kun Kit/GenTLE)

page 32 / 76


total genome

page 33 / 76


4

page 34 / 76


Hornbill

page 35 / 76


Feathers

page 36 / 76


use a single extractionsstep with chelex as aextraction medium

page 37 / 76


total genome

page 38 / 76


5

page 39 / 76


Insect

page 40 / 76


eggs

page 41 / 76


QIAamp® columns(Vallencia, CA)

page 42 / 76



page 43 / 76


6

page 44 / 76


cow

page 45 / 76


fecal

page 46 / 76


RBB+C

page 47 / 76


?

page 48 / 76


7

page 49 / 76


bear

page 50 / 76


blood

page 51 / 76


Qiam kit

page 52 / 76


total genome

page 53 / 76


8

page 54 / 76


chicken, goat,cattle and pigs

page 55 / 76


Fecal

page 56 / 76


QIAamp DNA stoolpurification kit

page 57 / 76



page 58 / 76


9

page 59 / 76


Human

page 60 / 76


Human fecal

page 61 / 76


- UltraClean Fecal DNAextraction kit- ExtractMaster Fecal DNAextraction kit

page 62 / 76



page 63 / 76


10

page 64 / 76


human

page 65 / 76


semen

page 66 / 76


FTA technology

page 67 / 76



page 68 / 76


page 69 / 76


page 70 / 76


page 71 / 76


page 72 / 76


Sumber:




4. DNA extraction,amplification and directsequensing from horbillfeathers. Philip A Morina,Jeane Messier, and DavidS woodroff; J.Sci.Soc.Thailand, 20 (1994)

page 73 / 76


31-345. Human and Insect

Mitochondrial DNAAnalysis fromMaggots. Jeffrey D.Wells, Ph.D.; FrancescoIntrona, Jr., M.D.;Giancarlo Di Vella, Ph.D.,M.D.; Carlo P.Campobasso, M.D.; JackHayes, Ph.D.; and FelixA. H. Sperling, Ph.D ;JOURNAL OF FORENSICSCIENCES (2001).




9. Comparison of methodsfor the extraction ofbacterial DNA fromhuman faecal samples

page 74 / 76


for analysis by real-timePCR. E.A Nelson1, 2, E.APalombo1, and S.RKnowles2; (2010).

10. 10. Application of FTAtechnology to extractionof sperm DNA frommixed body fluidscontainingsemen. Yoshihiko Fujita,Shin-ichi Kubo; Y. Fujita,S.-i. Kubo / LegalMedicine 8 (2006) 43–47

page 75 / 76

Resume Metode Ekstraksi mtDNA Vendi Eko Susilo...

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