Results of the 2015 Proficiency Tes7ng SchemeThe Pirbright Institute receives strategic funding...
Transcript of Results of the 2015 Proficiency Tes7ng SchemeThe Pirbright Institute receives strategic funding...
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www.pirbright.ac.ukThe Pirbright Institute receives strategic funding from BBSRC.
Resultsofthe2015ProficiencyTes7ngScheme
Anna B. Ludi1, Ginette Wilsden1, Lissie Henry1, Valerie Mioulet1, Britta Wood1, Ashley Gray1, Barsha Thapa, Bryony Armson1, Julie Maryan,
Sarah Belgrave1, Donald P. King1
FMDandSVDReferenceLaboratories
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Ensurecountriesareabletoiden7fyFMDintheircountry§ AchievedbyfeedbackonPTS,trainingandconsulta7on
Harmonisa7onamongstreferencelaboratories
§ Achievedbypresen7ngresultsonhowreferencelaboratoriescompare,distribu7onofreagentsandprotocols
PurposeofProficiencyTes7ngScheme(PTS)
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Par7cipants
63 Countries Worldwide
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Panel1–Infec7ousmaterialfromonecaseofsuspectedvesiculardiseaseforvirusdetec7on(epithelialsamples)
Panel2–Non-infec7ousmaterialforvirusgenome/an7gendetec7on
Panel3–Non-infec7ousmaterialforFMDserologynon-structuralandstructuralproteins
PanelsDistributed
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Panel1–laboratorytestsusedn=21
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VirusIsola7on An7genELISA rRT-PCR
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SampleIden7fica7on–O/SEA/Mya-98,O/SKR/6/2014
Interpreta7onrRT-PCR–allsamplescorrectlyiden7fied
VirusIsola7on–Onelaboratoryincorrectlyiden7fiedtwosamples
An7genELISA–Onelaboratoryincorrectlyiden7fiedasample
Discussion Cellsusedforvirusisola7on–IB-RS-2,lambkidney,bovinekidney,swinekidney,SK-6,BTY,LFBKαVβ6,BHK-21,ZZR-127
Panel1–Conclusion
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Panel2–laboratorytestsusedn=57
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An7genELISA rRT-PCR
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SampleIden7fica7on SVD/ITL/8/2008 Lineage-O/ME-SA/Ind-2001 O/SAU/1/2013 Lineage–O/SEA/Mya-98 O/SKR/6/2014 Lineage–A/Asia/Iran-05SIS-10 A/TUR/16/2014 Lineage–Asia1/Sind-08 Asia1/TUR/2/2013 Lineage–SAT2/VIIAlx-12 SAT2/EGY/24/2014
Interpreta7on An7genElisa–Sample5gaveambiguousresultsandsample6mistypedorwasinconclusiveincertainlabsrRT-PCR–Veryconsistentamongalllaboratories,onelaboratoryrequiredfollowup
Discussion TPIan7genELISAkitsarenolongeravailable.NewkitsareavailablethroughapartnershipwithIZSLER–pleasecontactpirbright.kitorders@pirbright.ac.ukformoreinforma7on.
Panel2–Conclusion
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Panel3–laboratorytestsusedn=58
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NSP PrioCHECK ID.Vet SPCE LPBE VNT
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Difficultyininterpre7ngELISAcross-reac7vity,par7cularlyLPBE
Discussion Importanceofknowingthelimita7onoftheassaysused
Howisitbeingusedinthefield?Istheserotypealreadyknown?
Panel3–Conclusion
Sample 8
4 mistyped as As1 (1 lab no A system) 1 mistyped as O 2 mistyped as SAT 2 (1 lab no O system) 2 mistyped as SAT 3 (1 lab no A system) 2 positive - unknown serotype
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Panel1:Outbreakscenariosofvesiculardiseases(FMDand/orSVD)(infec'ousandnon-infec'ous) Useyourna7onal con7ngencyplanor similardocument to iden7fy theappropriate testsandtes7ngsequence.Thisexerciseisprimarilytoevaluateyouroveralldiagnos7cprocedureratherthan individual test methods. Please use downstream characterisa7on methods, such assequencingiftheyarepartofyourcon7ngencyplan.
Example-Panel1a-Anownerhasreportedsuspicionoffootandmouthdisease,followingashort history of severe lameness and milk drop in one dairy cow, and the development ofblistersinthemouthsoftwocalvesthatarekeptnearby.Thesamplesofthetwocalveshavebeensenttoyourlaboratoryforinves7ga7on.
Panel 2: Quality Assurance FMDV samples to ensure recent strains are detected (non-infec7ous)
ThispanelofvirusesrepresentsrecentFMDVisolatesthatposeacurrentthreattotheEUandelsewhere.Theaimoftheevalua7onistomakesuretheselateststrainscanbedetected.
Panel3:Serologicalsamplesfromanoutbreakscenarioinpanel1 Example–Panel3a–Theoutbreakinscenarios1aand1bhavenowbeencontained.Threeserawerecollectedpostvaccina7on.Pleasetestthemandcommentastowhetherthesamplesarefrominfectedanimals,andifnot,whetherthereisanyevidenceofvaccine-inducedimmunity.
Changestothe2016PTS
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Acknowledgement
WorldReferenceLaboratory
CentralServiceUnit
ProficiencyTes7ngSchemeAdvisoryBoard
Youforpar'cipa'nginthePTSandlisteningtothispresenta'on