Resins for Reverse-Phase Chromatography, Adsorption and Solid …58bc8b99-bd4c-4a1b-b787... ·...

Resins for Reverse-Phase Chromatography, Adsorption and Solid Phase Extraction (SPE)

ChromaliteTM

Chromalite for Reverse-Phase Chromatography, Adsorption and SPE | 32 | Chromalite for Reverse-Phase Chromatography, Adsorption and SPE

Why choose Purolite?

Since 1981, Purolite has grown into the world’s premier resin-based separation, purification and extraction technology manufacturer and innovation leader, with manufacturing facilities, advanced research laboratories and over 1100 people employed world-wide.

Purolite brings innovative thinking and a distinguished history of resin technology expertise to the global Life Sciences marketplace.

We provide APIs, enzyme carriers, immobilized enzymes, and chromatographic resins of the highest quality, to support research and development and production-scale applications in global pharmaceutical, food and beverage, bioprocessing, cosmetics and fine chemical markets.

Contents

Why choose Purolite? 3Quality 4Security of supply 4Introduction to Chromalite 5Selecting the right resin for your application… 6How are Chromalite manufactured? 7Silica – not always the optimal choice 8Reverse-phase chromatography 10Adsorption 11Solid phase extraction (SPE) 11Chromalite AD 13Chromalite PCG 15Chromalite PCG1200MHEMA and 70MN 21Chromalite GN 25Regulatory compliance 27Ordering information 29

We bring innovative thinking and resin technology expertise to the global Life Sciences marketplace

THIS IS AN INTERACTIVE DOCUMENT

Clicking on the section in the table of contents will take you to that page.

Product range Chromalite Styrene / DVB

Chromalite Methacrylate

Praesto® Agarose

Particle size (micron) 5 – 300 40 – 300 35 – 90

Matrix Styrene / DVB Methacrylate Agarose

Method of separation RP / IEX* IEX / Affinity IEX / Affinity

Method of elution Organic solvents / salt Organic solvents / buffers /salt

Buffers /salt

Max. pressure 50 bar 10 bar 4 bar

Typical target molecule 100 Da – 50 kDa 1 – 250 kDa 20 – 500 kDa

Chromalite for Reverse-Phase Chromatography, Adsorption and SPE | 5

QualityPurolite maintains a global Quality Management System (QMS) which supports BSI requirements of ISO 9001. Compliance is monitored and maintained through a quality assurance and regulatory team, who conduct internal audits to ensure operations meet the guidelines and protocols for equipment and procedures. Our teams are given continuous training on quality processes to ensure batch-to-batch consistency, and the highest product quality.

Security of supplyEnsuring reliable availability of our resins is vital to customers, and of paramount importance to Purolite. As a leading supplier of resin to the world’s most regulated industries, we recognize that our resins are critical purification products.

As such, a real-world security-of-supply system is in place to support your process requirements for business continuity. Supply risk is managed end-to-end, with a global network of qualified suppliers. Long-term supply agreements with periodic audits ensure consistency and ‘fit for purpose’ performance. Purolite has manufacturing facilities at 4 strategic locations in the USA, Asia and Europe. Lifetech™ and Chromalite™ products are manufactured at both UK and Romania-based facilities, which are fully certified under ISO 9001:2015, ISO 14001:2015 and ISO 45001:2018. Our Romanian facility is FDA-inspected, with a total of 4 state-of-the-art clean rooms, offering separate facilities for ligand/ enzyme immobilization, removal of fines, solvent or purified water washing, screening, vacuum drying and packaging.

Chromalite chromatography products are produced using a Purolite-patented jetting technology, developed by our UK-based R&D team. These products are manufactured both in our UK-based pilot facility and at full production scale in Romania. Our highly qualified scientists have the technical expertise, experience and equipment to produce uniform bead gels and macroporous products in the range of 5-1000μm in diameter, with uniformity coefficients as low as 1.1. This narrow particle size distribution is vital for effective chromatographic purification.

* Chromalite styrene/DVB ion exchange resins are described in detail in a separate technical brochure, ‘Chromalite CGA and CGC Ion Exchange Resins’ which can be found on www.purolite.com.

IntroductionKey features

100% focused on resin technology.

Global manufacturing at facilities in the UK, Romania, China and USA.

ISO-certified, state-of-the-art manufacturing facilities.

30+ years of regulatory experience from FDA-inspected cGMP facility.

40+ years of experience in solving advanced R&D and purification challenges.

The Chromalite chromatography product range provides a comprehensive line of products for a variety of applications. This brochure focuses on Chromalite AD, PCG, MN and GN polymeric resins designed for adsorption, reverse-phase chromatography (RPC) and solid phase extraction (SPE). These rigid, polymeric resins are extensively used for analysis and purification.

Our Chromalite resins feature the most desirable properties of modern chromatography resins including mechanical robustness, inertness, pH stability and flexibility, through their compatibility with organic solvents and aqueous solutions. The range of particle sizes provides scalability from analytical to industrial-scale purification and the Chromalite range also features a selection of porosities, surface areas and chemistries, designed to meet the needs of customers in a range of industries. Chromalite resins are currently being used for the purification and analysis of amino acids, peptides, small proteins, organic acids, carbohydrates and inorganic cations and anions.

Highly Hydrophobic

Medium Hydrophobic

Highly Hydrophilic

4 | Chromalite for Reverse-Phase Chromatography, Adsorption and SPE


Selecting the right resin for your application…All Chromalite resins are hydrophobic, robust and have excellent chemical stability. They are highly stable to organic solvents and show minimal swelling when changing between different solvents and salts. You have greater flexibility in the choice of elution conditions and cleaning regimes, including the use of sodium hydroxide or hydrochloric acid, which increases sample throughput and the number of cycles achieved per column.

All resins are stable from pH 1-14 and are highly stable to organic solvents, showing minimal swelling when changing between different solvents and salts.

Use the below (Table 1, 2 and 3) to see which characteristics differentiate Chromalite resins.

Excellent chemical stability

Minimal swelling

Hydrophobic

Robust

3

3

3

3

AD PCG MN GN

Base matrix Macroporous polystyrene /divinylbenzene

PCG600, PCG900 & PCG1200

Macroporous polydivinylbenzene

Polystyrene Polystyrene/ divinylbenzene

PCG950 Macroporous polymethacrylate

PCG1200MHEMA Hydroxyethyl methacrylate/DVB

Particle size range (micron)

5 – 30 35 – 150 70 3 – 50

Manufacturing method

Seeding Jetting Seeding Seeding

Technique RP-HPLC Adsorption

RP-low pressure chromatography

Solid phase extraction

Adsorption

RP-HPLC


HPLC

Calibration

Info on page 13 Info on page 15 Info on page 21

Info on page 25

Table 1 General characteristics and applications of Chromalite AD, PCG, MN & GN

How we manufacture our resins

Jetting is our proprietary manufacturing technology which produces resins with a very narrow particle size distribution, providing enhanced performance and column packing consistency.

Typical particle size range: 50 – 250 μm Uniformity Coefficient: <1.3

Seeding creates uniform particle size beads with a monodispersed particle size distribution. Small ‘seeds’ are subsequently grown through repeated additional polymerization.

Typical particle size range: 3 – 70 μm Uniformity Coefficient: <1.15

Table 2 Overview of Chromalite products and their manufacturing process

Table 3 Chromalite applications depending on resin particle size

Mean particle size

5 µm 10 µm 15 µm 30 µm 35 µm 50 µm 70 µm 75 µm 125 µm

Macroporous reverse phase

5AD2 10AD2 15AD2 30AD2 PCG600F PCG900F PCG1200F PCG950F

70MN PCG600M PCG900M PCG1200M PCG950M PCG1200MHEMA

PCG600C PCG900C PCG1200C PCG950C

Unfunctionalized DVB cross-linked gel beads

GN

Chromalite mean diameter (µm) Application

5 Analytical HPLC

10, 15 Analytical/Preparative RP-HPLC

Process/polishing of small molecules and synthetic biomolecules

30, 35 Process/polishing of synthetic biomolecules

50, 75 Low-pressure chromatography, front-end capture, desalting, and purification applications of small organic molecules and synthetic biomolecules (e.g. peptides and oligonucleotides)


75, 125 Large-scale purification of small organic molecules and synthetic biomolecules

Macroporous polystyrene/divinylbenzene Macroporous polydivinylbenzene

Macroporous polymethacrylate Hydroxyethyl methacrylate/DVB

Polystyrene Polystyrene/divinylbenzene

KEY


MN 70µm

Silica – not always the optimal choice

Polymeric resins are becoming more frequently used in both established modern liquid chromatography processes and the discovery of new applications.

Modern polymers have now overcome the teething problems many users encountered during their initial introduction to the market. Thanks to radical improvements in both resin design and manufacturing technology in recent years, polymeric resins such as Chromalite now offer distinct advantages over silica, such as better stability at extreme pH conditions and reduced sensitivity to metals and buffer salts.

Polymer-based resins provide reliable performance and excellent thermic and chemical stability in all commonly-used solvents for chromatography and solid phase extraction. The high rigidity and mechanical strength of Chromalite polymers increases column lifetime and allows the use of high eluent flow rates for faster analysis. Chromalite resins achieve efficiencies exceeding 100,000 plates/m.

The relative sizes of particles

Width of a human hair

Fine beach sand

Grain of salt

White blood cell

Grain of pollen

Dust particle (PM10)

Red blood cell

Respiratory droplets

Dust particle (PM2.5)

Bacterium

Wildfire smoke

Coronavirus

T4 Bacteriophage

Zika Virus

50 – 180µm

90µm

60µm

25µm

15µm

<10µm

7 – 8µm

5 – 10µm

2.5µm

1 – 3µm

0.4 – 0.7µm

0.1 – 0.5µm

0.225µm

0.045µm

AD 5 – 30µm

PCG 35 – 125µm

GN 3 – 50µm


Reverse-phase chromatography

Figure 1 Adsorption and elution process in high pressure chromatography

Elution from hydrophobic surface

Absorption viahydrophobicinteraction

Elution by increasing

organic solvent concentration

Hydrophobic molecules

Reverse-phase chromatography is a proven technique for the purification of biomolecules and results from the adsorption of molecules onto a hydrophobic solid support in a polar mobile phase. Decreasing the mobile phase polarity, typically by increasing the proportion of a polar organic solvent, increases the affinity of the bound material for the mobile phase (Figure 1). The more hydrophobic the molecule, the stronger the interaction with the solid support and the higher the proportion of organic solvent required to promote desorption.

• RPC applies to a very wide range of hydrophobic molecules

• Variables such as organic solvent type and concentration, pH and temperature are used to optimize and control separation in RPC

• Once the stationary phase is packed in a column, RPC columns are robust, efficient and stable

• RPC media are robust and tolerate a wide range of pH, the use of harsh chemicals, a wide temperature range, and are easily regenerated and cleaned

At process scale, RPC is applied to organic compounds like APIs and biomolecules like peptides and oligonucleotides (Figure 1). RPC is rarely used for purification of larger biomolecules since the presence of the organic solvent can cause denaturation of proteins and destroy their biological activity. For purification of proteins, more hydrophilic matrices like agarose or acrylates are generally preferred. There are exceptions, such as the successful industrial purification by reverse phase of insulin, which will be described later. Reverse-phase chromatography is the most common analytical HPLC technique. There are many different stationary phases available for method optimization, including silica functionalized with hydrophobic groups (e.g. C18, C8 silica) or the more robust synthetic polymeric phases like Chromalite.

Adsorption

Hydrophobic resin saturated with solvent

Solvent flowsout of column

Solvent

Mixture containingcomponents: X and Y

Y moves down thecolumn more quicklythan X and so leaves the column first

1 2

X

Adsorption and desorption are the main processes operating in chromatography. It is the relative rates of adsorption and desorption onto and off the stationary phase that allows for molecules of interest in samples to be separated (Figure 2).

If the column conditions favour adsorption of a molecule, then the molecule will adhere to the stationary phase and be separated from the other sample chemicals. When the conditions favour desorption, the opposite will happen — and the chemicals will be released into the mobile phase.

Solid phase extraction is an extractive technique by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties (Figure 3). Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. Solid phase extraction can be used to isolate analytes of interest from a wide variety of matrices, including urine, blood, water, beverages, soil, and animal tissue.

SPE uses the affinity of solutes dissolved or suspended in a liquid through which the sample is passed to separate a mixture into desired and undesired components. The result is that either the desired analytes of interest or undesired impurities in the sample are retained on the stationary phase.

Solid phase extraction (SPE)

Conditioning Loading Washing Elution

Figure 2 Adsorption and elution process in low pressure chromatography

Figure 3 Typical process for SP capture and elution of compounds on hydrophobic resins (RP-SPE)


Chromalite AD

Applications include:

• RP-HPLC


Chromalite AD

Features

• Highly hydrophobic • High chemical stability • More robust than silica materials • Easy column packing • Efficient regeneration • Narrow particle size

Table 4 Chromalite AD2 macroporous polystyrene/divinylbenzene adsorbents manufactured by seeding

Figure 4 Uniform particle size distribution of 10AD2 Figure 5 Controlled porosity of 15AD2

Chromalite AD are characterized by outstanding chemical and physical stability. Since they are manufactured by seeding and therefore have small particle size and very narrow particle size distribution (UC < 1.15), their main application is analytical and preparative HPLC. They are available in 5, 10, 15 and 30 micron sizes and figures 4 & 5 show the uniform particle size and porosity obtained by seeding.

Chromalite 5AD2, 10AD2, 15AD2 and 30AD2 (Table 4) are highly hydrophobic and are an excellent alternative to C18 or C8 silica matrices. Chromalite AD resins are characterized by a porosity of 200-300Å and are ideal for separation of small/medium molecules with MW < 15 kDa, and can substitute C8/C18 silica with porosity of 50 – 100Å. Chromalite AD2 are ideal resins to be used in the separation and purification of synthetic oligomers, biomolecules, peptides, small proteins and oligonucleotides.

a Supplied as 20% EtOH slurry

Producta Particle size (µm) Surface area (m2/g)

5AD2 5 >500

10AD2 10 >500

15AD2 15 >500

30AD2 30 >350

All the AD2 resins have a pore diameter of 200 – 300 Å and a bulk density of 0.2 – 0.25 gdry/mL of bed volume.

Chromalite PCG


• Adsorption

• RP-low pressure chromatography

• Solid phase extraction


Chromalite PCG

Features

• Highly hydrophobic• High chemical stability• More robust than silica materials• Easy column packing• Efficient regeneration• Narrow particle size

Chromalite PCG resins are characterized by very high hydrophobicity. These resins are manufactured by our patented jetting technology to create a narrow particle size distribution for unrivalled uniformity (Figure 6 and 7). This uniformity means that Chromalite PCG resins can be used for medium and low-pressure RPC. The PCG resins are compatible with polar aqueous protic and aprotic eluents and show minimal swelling from one solvent to the other. The grading given to these resins refers to their available particle size:

• 20 – 50 micron (F grade) • 50 – 100 micron (M grade) • 100 – 200 micron (C grade)

Figure 6 Optical photographs of PCG900M (50-100 micron – M grade resin)

Figure 7 Optical photographs PCG1200C (100 – 200 micron – C grade resin)

Table 5 Chromalite PCG macroporous polydivinylbenzene adsorbents manufactured by jetting

Different porosities enable Chromalite PCG resins to accommodate a variety of molecule sizes and be used for different applications.

• High porosity: Chromalite PCG1200 with a porosity of 300 – 500 Å • Medium porosity: Chromalite PCG900 with a porosity of 150 – 300 Å • Low porosity: Chromalite PCG600 with a porosity of 75 – 150 Å

Typical applications for Chromalite PCG are adsorption and reverse-phase chromatography for the purification of biomolecules after fermentation as well as applications in blood and plasma purification for medical devices and cannabinoid purification.

Chromalite PCG600, PCG900 and PCG1200 are ideal resins for reverse-phase chromatography and adsorption processes. Elution usually requires an organic cosolvent. The more hydrophobic the molecule, the stronger the interaction with the solid support and the higher the proportion of organic solvent required to promote desorption. Chromalite PCG resins are compatible with either polar aqueous protic and aprotic eluents and show minimal swelling from one solvent to the other (< 20% in 20% ethanol). They can be used over the entire pH range 1-14 (Table 5).

a Supplied as 20% EtOH slurry b Supplied wet

Product Particle size (µm) Pore diameter (Å)

PCG600Fa 20 – 50 75 – 150

PCG600Mb 50 – 100 75 – 150

PCG600Cb 100 – 200 75 – 150

PCG900Fa 20 – 50 150 – 300

PCG900Mb 50 – 100 150 – 300

PCG900Cb 100 – 200 150 – 300

PCG1200Fa 20 – 50 300 – 500

PCG1200Mb 50 – 100 300 – 500

PCG1200Cb 100 – 200 300 – 500

All of these PCG resins have a surface area of >600 (m2/g) and bulk density of between 0.20-0.23 (dry) / 0.73-0.78 (wet) g/mL of bed volume.


Chromalite PCG950

Chromalite PCG950 is a hydrophilic, highly crosslinked, porous polymethacrylic adsorbent. The pore size and matrix are designed for adsorption and reverse-phase chromatography purification of biomolecules including small proteins, peptides, antibiotics, nucleic acids and other small molecules.

Chromalite PCG950 is more hydrophilic than PCG600, PCG900 and PCG1200 due to its aliphatic structure and a structure containing oxygen.

It is an excellent technical and economical choice for high-resolution low-pressure chromatography, suited for operation within the entire pH range and for cleaning in place with most organic solvents and dilute acids and base (Table 6).

Chromalite PCG950 resins are compatible with polar aqueous/alcoholic eluents. Selecting a concentration of organic alcohol to elute depends on two factors for optimization:

– Low run time for higher productivity - optimal run time is achieved at over 50% organic solvent

– Better separation for higher purity - optimal separation is achieved at 10% organic solvent

Chromalite PCG950 is characterized by a porosity of 150-300Å which is optimal to accommodate biomolecules up to 15 kDa.

Chromalite PCG950 resins are manufactured by jetting to generate a narrow particle size distribution with UC < 1.3 (Figure 8). Thanks to this uniformity, Chromalite PCG950 resins can be used for medium- and low-pressure RP-LC due to the particle sizes offered:

• 20 – 50 micron (F grade) • 50 – 100 micron (M grade) • 100 – 200 micron (C grade)

Table 6 Chromalite macroporous polymethacrylic adsorbent manufactured by jetting

a Supplied as 20% EtOH slurry b Supplied wet

Figure 8 Electron microscopy of PCG950F

Product Particle size (µm)

PCG950Fa 20 – 50

PCG950Mb 50 – 100

PCG950Cb 100 – 200

All of these PCG950 resins have a surface area of >500 (m2/g), pore diameter of 150 – 300 Å and bulk density of between 0.20-0.23 (dry) / 0.73-0.78 (wet) g/mL of bed volume.

Swelling of PCG600 in ethanol

Ethanol Concentration (%)

Re

lati

ve V

olu

me

(%

)

115

110

105

100

95

90

85

80

75

0 20 40 60 80 100

PCG950M PCG950C

Re

lati

ve V

olu

me

(%

)

120

100

80

60

40

20

0Water Methanol Ethanol Isopropanol

Solvent

Acetonitrile Acetone Dry

Figure 9 Swelling of PCG600M in ethanol

Macroporous resins, based on either divinylbenzene or methacrylate, are highly rigid and do not show significant swelling in organic solvent. PCG600 shows an increase of swelling of less than 15% in full ethanol (Figure 9).

Figure 10 Comparison of swelling in different organic solvents of PCG950M and PCG950C

PCG950 is a methacrylate-based resin, more hydrophilic than PCG600, PCG900 and PCG1200. The hydrophilic nature and the rigidity of the resin minimizes the swelling from water to organic solvents. This is a great advantage in RP applications using packed columns where organic solvents are required for elution of target compounds (Figure 10).


Chromalite PCG1200MHEMA and 70MN


• Adsorption

• RP-low pressure chromatography



Chromalite PCG1200MHEMA and 70MN

The Chromalite range also features two specialist products, PCG1200MHEMA and 70MN, that have been specifically designed for solid phase extraction (SPE) (Table 7).

PCG1200MHEMA is a macroporous copolymer of hydroxyethyl methacrylate (HEMA) with divinylbenzene and differs from the other PCG resins as the content of hydroxymethyl methacrylate in the matrix gives more hydrophilicity, making this product particularly tailored to SPE applications. A distinct feature of PCG1200MHEMA is its wettability which allows for the pre-concentration of analytes from water media directly onto the adsorbent without pre-washing the resin (Figure 11).

70MN is a hyper-crosslinked macroporous copolymer with a hydrophobic polystyrene matrix that is water wettable. It is characterized by an extremely high surface area (> 1200m2/g) that increases its adsorption properties towards hydrophobic molecules. Chromalite 70MN is manufactured using our patented seeding technology and the micropores created in the range of 20–50 Å are responsible for the adsorption of small analytes that can be selectively absorbed from drainage waters, blood, urine etc. by size exclusion mechanisms (Figure 12 and Figure 13). The specific structure of 70MN makes this resin ideal for SPE due to the high selectivity towards analyzed components.

Figure 11 Optical photograph of PCG1200MHEMA (75 micron) Figure 12 Optical photograph of 70MN (70 micron)

Figure 13 Porosity Profile for 70MN

Features

• High chemical stability• Efficient regeneration• Large surface area• Narrow particle size range• Excellent selectivity• Low extractable content• Easy column packing• More robust than silica materials

Table 7 Chromalite resins specifically designed for SPE

a Supplied as dried resin b Micropores

Pore Diameter (Å)

Po

re V

olu

me

(m

L/g

)

0 25 50 75 100

Product Particle size (µm) Surface area (m2/g) Pore diameter (Å) Bulk Density (g/mL of bed volume) – dry /wet

70MNa 70 > 1200 20 – 50b 0.4 – 0.5

PCG1200MHEMAa 50 – 100 > 500 200 – 400 > 0.22


Chromalite GN


• Adsorption

• RP-HPLC



Chromalite GN

Features

• High chemical stability• Narrow particle size range• Suitable for further functionalization

for HPLC applications

Chromalite GN resins are highly uniform non-porous beads with different levels of divinylbenzene cross-linking.

They are manufactured by our patented seeding process to create different particle sizes ranging from 3 micron to 50 micron. They are very narrow in particle size to suit their intended application in the calibration and validation of analytical equipment or sizing instruments (Figure 14).Their main application is calibration purposes and further functionalization to create IEX-HPLC chromatographic resins.

Depending on the cross-linking factor, each product displays different rigidity and swelling in solvents which favor different ion exchange capacities following further functionalization.

All Chromalite GN products are supplied as dry resins.

Figure 14 Electron scan microscopy for 10GN8 (10 micron gel type cross-linked with 8% DVB) showing the monodispersity (in green particle size measurement).

Regulatory compliance

Table 8 Regulatory compliance for Chromalite resins

* No for products delivered as 20% ethanol slurry (F grade), Yes for products delivered as wet (M and C grade)

The regulatory environment is ever changing, driven by increasing regulatory requirements, increasing development costs and times, and market pressures impacting pharma and food industries. Reg ulatory issues influence decision-making throughout the product life cycle - at every stage of development, distribution, marketing and post-market monitoring. Consequently, regulatory manage ment has a role in many business-critical functions, including organizational and corporate strategy, technology assessment, research and development, and legal issues.

Regulatory expertise throughout the product life-cycle is essential to identify regulatory options for product development, optimize ‘speed to market’ and produce a product that meets customer needs. Purolite implements control documentation and processes at every level to ensure regulatory support to customers using our products. Our raw material suppliers are selected and qualified from leading manufacturers and are part of our network suppli er program. Raw materials suppliers and partners are routinely audited for compliance to quality standards. Critical raw materials are managed through a globally coordinated inventory system to ensure security of supply. Additionally, a quality control protocol is in place for testing new batches/lots of raw materials to confirm product specifications and lot-to-lot consistency.

Global ISO 9001: 2015 standards ensure consistent operating practices across each of our plants. Compliance is monitored and maintained through a quality assurance and regulatory team who conduct internal audits to ensure operations meet the guidelines and protocols for equipment and procedures. Additionally, our production team is given continual training on quality processes to ensure batch-to-batch consistency. Quality certificates for our sites are available, and we welcome customer visits and host nu merous audits each year to make sure we meet and comply with user and regulatory expectations.

Products are shipped in compliant containers and under con trolled conditions, as applicable. Certificates of analysis (CoA) and conformance of materials records are available for all Purolite products. These documents comply with product release criteria, certify animal-free origin, or show wetted materi als are in conformance with USP class VI.

For products used in the life sciences applications, Regulatory Support Files (RSF) are available on demand. Regulatory Support Files provide direct and detailed information on performance, stability, extractable compounds, and analytical methods for each resin.

Product Name

Compliant with

21CFR17 3.25

21CFR17 3.65

21CFR177. 2710

Re-sAP(2004)3

Halal Kosher GMO Free

TSE/BSE Free

AD – 3 – 3 5* 3 3 3

PCGPCG600, PCG900, PCG1200

– 3 – 3 5* 3 3 3

PCG950 – – – 3 5* 3 3 3

PCG1200M-HEMA – – – 3 3 3 3 3

MN – – 3 3 3 3 3 3

GN – – 3 3 3 3 3 3


Ordering Information

To place your order simply contact us via email or telephone and quote the product codes found in the following tables.


Resin range Shipped as Quantity Container

AD 20% EtOH slurry 10 g 75 ml bottle

50 g 250 ml bottle

100 g 1 L bottle

250 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle

2-10 Kg 10 L Jerrycan



PCG600F 20% EtOH slurry 10 g 75 ml bottle

50 g 250 ml bottle

100 g 1 L bottle

250 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle




PCG600M

PCG600C

Wet resin (water) 50 g 50 g tub

250 g 250 g tub

1 Kg 1 Kg tub

5-25 Kg 5 Kg tub

25-100 Kg 60 L keg/Foil bag



50 g 250 ml bottle

100 g 1 L bottle

250 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle





PCG900M

PCG900C


250 g 250 g tub

1 Kg 1 Kg tub

5-25 Kg 5 Kg tub




50 g 250 ml bottle

100 g 1 L bottle

250 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle




PCG950M

PCG950C


250 g 250 g tub

1 Kg 1 Kg tub

5-25 Kg 5 Kg tub




100 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle




PCG1200M

PCG1200C


1 Kg 1 Kg tub

5-25 Kg 5 Kg tub



Bulk resin packaging options



PCG1200MHEMA Dry resin 10 g 75 ml bottle

100 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle

2-10 Kg 5 Kg tub

10-20 Kg 5 Kg tub

20-100 Kg 5 Kg tub

70MN Dry resin 50 g 250 ml bottle

100 g 250 ml bottle

250 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle

2-10 Kg 5 L bottle

GN Dry resin 10 g 75 ml bottle

50 g 250 ml bottle

100 g 1 L bottle

250 g 1 L bottle

500 g 1 L bottle

1 Kg 5 L bottle

Ordering information

AD

PCG

Product Order number

5AD2 LS06950

10AD2 LS06840

15AD2 LS06890

30AD2 LS06870


PCG600F LS04740

PCG600M LS04750

PCG600C LS04760

PCG900F LS04770

PCG900M LS04780

PCG900C LS04790

PCG1200F LS06720

PCG1200M LS06725

PCG1200C LS06730

PCG950F LS05000

PCG950M LS05010

PCG950C LS05020

PCG1200MHEMA LS06700

Bulk Resins


Ordering information

Product Column size Order number

5AD2 HPLC column 4.6 mm x 150 mm LS06950-601

4.6 mm x 250 mm LS06950-602

10 mm x 150 mm LS06950-603

10 mm x 250 mm LS06950-604

21.2 mm x 50 mm LS06950-605

21.2 mm x 100 mm LS06950-606

21.2 mm x 150 mm LS06950-607

21.2 mm x 250 mm LS06950-608

30 mm x 50 mm LS06950-609

30 mm x 100 mm LS06950-610

30 mm x 150 mm LS06950-611

30 mm x 250 mm LS06950-612

50 mm x 50 mm LS06950-613

50 mm x 250 mm LS06950-614


4.6 mm x 250 mm LS06840-602

10 mm x 150 mm LS06840-603

10 mm x 250 mm LS06840-604

21.2 mm x 50 mm LS06840-605

21.2 mm x 100 mm LS06840-606

21.2 mm x 150 mm LS06840-607

21.2 mm x 250 mm LS06840-608

30 mm x 50 mm LS06840-609

30 mm x 100 mm LS06840-610

30 mm x 150 mm LS06840-611

30 mm x 250 mm LS06840-612

50 mm x 50 mm LS06840-613

50 mm x 250 mm LS06840-614

HPLC columns (Stainless steel)

MN

GN


70MN LS06910


3GN6 LS03003

4GN8 LS06820

5GN6 LS03005

5GN8 LS03305

6GN6 LS03006

6GN8 LS06815

7GN8 LS03307

8GN6 LS06800

8GN8 LS06800

10GN6 LS03010

10GN8 LS03310

15GN6 LS03015

20GN4 LS03024

20GN6 LS03026

25GN6 LS03030

30GN6 LS03036

30GN3 LS03043

32GN6 LS03056

50GN8 LS03350




4.6 mm x 250 mm LS06890-602

10 mm x 150 mm LS06890-603

10 mm x 250 mm LS06890-604

21.2 mm x 50 mm LS06890-605

21.2 mm x 100 mm LS06890-606

21.2 mm x 150 mm LS06890-607

21.2 mm x 250 mm LS06890-608

30 mm x 50 mm LS06890-609

30 mm x 100 mm LS06890-610

30 mm x 150 mm LS06890-611

30 mm x 250 mm LS06890-612

50 mm x 50 mm LS06890-613

50 mm x 250 mm LS06890-614


PCG600M 200ml column 36 mm x 202 mm LS04750-615

PCG600C 200ml column 36 mm x 202 mm LS04760-615







PCG 200 mL column KIT

Contains:

PCG600M 200ml Column




36 mm x 202 mm LS04610-KIT

Flash chromatography columns (polypropylene)

38 | Chromalite for Reverse-Phase Chromatography, Adsorption and SPE

AlgeriaAustraliaBahrainBrazilCanadaChinaCzech RepublicFranceGermany

IndiaIndonesiaIsraelItalyJapanJordanKazakhstanKoreaMalaysia

MexicoMoroccoNew ZealandPolandRomaniaRussiaSingapore Slovak RepublicSouth Africa

SpainTaiwanTunisiaTurkeyUKUkraineUSAUzbekastan

AmericasPurolite Corporation2201 Renaissance Blvd. King of Prussia, PA 19406T +1 800 343 1500T +1 800 668 9090 F +1 800 260 1065 [email protected]

EMEAPurolite Ltd Unit D Llantrisant Business ParkLlantrisant, Wales, UKT +44 1443 229334 F +44 1443 227073 [email protected]

FSU Purolite Ltd. Office 6–1 36 Lyusinovskaya Str. Moscow, Russia 115093 T +7 495 363 5056 F +7 495 564 8121 [email protected]

Asia Pacific Purolite China Co. Ltd. Room 707, C Section Huanglong Century Plaza No. 3 Hangda Road Hangzhou, Xhejiang, China 310007 T +86 571 876 31382 F +86 571 876 31385 [email protected]

Purolite, the leading manufacturer of quality ion exchange, catalyst, adsorbent and specialty high-performance resins, is the only company that focuses 100% of its resources on the development and production of resin technology.

We’re ready to solve your process challenges. For further information on Purolite products and services, visit www.purolite.com or contact your nearest Technical Sales Office.

www.purolite.com

©2021 Purolite Corporation All rights reserved.

The statements, technical information and recommendations contained herein are believed to be accurate as of the date hereof. Since the conditions and methods of use of the product and of the information referred to herein are beyond our control, Purolite expressly disclaims any and all liability as to any results obtained or arising from any use of the product or reliance on such information; NO WARRANTY OF FITNESS FOR ANY PARTICULAR PURPOSE, WARRANTY OF MERCHANTABILITY OR ANY OTHER WARRANTY, EXPRESSED OR IMPLIED, IS MADE CONCERNING THE GOODS DESCRIBED OR THE INFORMATION PROVIDED HEREIN. The information provided herein relates only to the specific product designated and may not be applicable when such product is used in combination with other materials or in any process. Nothing contained herein constitutes a license to practice under any patent and it should not be construed as an inducement to infringe any patent and the user is advised to take appropriate steps to be sure that any proposed use of the product will not result in patent infringement.

Resins for Reverse-Phase Chromatography, Adsorption and Solid …58bc8b99-bd4c-4a1b-b787... ·...

Documents

Transcript of Resins for Reverse-Phase Chromatography, Adsorption and Solid …58bc8b99-bd4c-4a1b-b787... ·...