Research & reviews journal of veterinary science and technology (vol3, issue2)

ISSN 2349-3690RRJoVST

May-August 2014

Veterinary ScienceResearch & Reviews: Journal of

and Technology

�Antioxidant Enzyme Status in Broiler Chickens

�Biochemical Assessment of Allantoic Fluid in Pregnant Sheep

�Biochemical Changes in Pregnant and Lactating Kilakarsal Ewes

�Role of Pheromones & Biostimulation in Animal Reproduction

�Treatment of Mange in Rabbits: A Case Report

�Torsion of Uterus as a Cause of Dystocia

� Mortality Due to Pasteurellosis in Calves

STM JOURNALSScientific Technical Medical



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Prof. Dr. Hazim Jabbar Al-DarajiProfessor University of Baghdad,

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Pathology, College of Veterinary Science, Korutla A.P. India

Prof. Dr. Ravi kumar PentelaProfessor Dept. of Pharmacology& amp; Toxicology, NTR College of Veterinary Science, Annavaram, Andhra Pradesh

India - 521 102

Editorial Board

Dr. Ashok KumarAssociate Professor, Department of Vety. Medicine LLR University of

Veterinary & Animal Sciences, HisarIndia.

Dr. Sandeep Kumar GuptaScientist , Lala Lajpat Rai, Univ of

Veterinary and Animal Sciences, Hisar India.

Dr. Shamsul Haque Professor & Head

Department of Veterinary MedicineBirsa Agriculture University, Ranchi

India.

Dr. Rashmi Rekha Kumari Assistant Profesor, Department of Veterinary

Pharmacology & ToxicologyBihar Veterinary College, Patna

India.

Dr. Pankaj SinghBihar Veterinary College

Bihar Agricultural UniversityBihar, India.

Dr. Girish Kumar MishraAssistant Professor College of Veterinary

Science and Animal Husbandry,Chhattishgarh Kamdhenu Vishwavidyalaya India.

Dr. Gopala ReddyProfessor & University Head, Dept of

Veterinary Pharmacology & Toxicology, College of Veterinary science,

Rajendranagar, Hyderabad India.

Dr. Kaushalendra KumarAssistant Professor,

Department of Animal NutritionBihar Agricultural University,

India.

Dr. Aditi Dixit Assistant Professor,

College of Veterinary Sci.Durg (C.G.)

India.

Dr. Gyandev SinghAssistant professor,

clinical complex, B.V.C PatnaIndia.

I take the privilege to present the hard copy compilation for the [Volume 3 Issue (2)] of

Research&Reviews: Journal of Veterinary Science and Technology (RRJoVST).The intension of

RRJoVST is to create an atmosphere that stimulates creativeness, research and growth in the area of

Veterinary Science and Technology.

The development and growth of the mankind is the consequence of brilliant Research done by

eminent Scientists and Engineers in every field RRJoVST provides an outlet for Research findings

and reviews in areas of Veterinary Science and Technology found to be relevant for National and

International recent developments & research initiative.

The aim and scope of the Journal is to provide an academic medium and an important reference for

the advancement and dissemination of Research results that support high level learning, teaching and

research in the domain of Veterinary Science and Technology

Finally, I express my sincere gratitude and thanks to our Editorial/ Reviewer board and Authors for

their continued support and invaluable contributions and suggestions in the form of authoring write-

ups/ reviewing and providing constructive comments for the advancement of the journals. With

regards to their due continuous support and co-operation, we have been able to publish quality

Research/Reviews' findings for our customers base.

I hope you will enjoy reading this issue and we welcome your feedback on any aspect of the Journal.

Dr. Archana Mehrotra

Director

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Director's Desk

STM JOURNALS

1. Antioxidant Enzyme Status in Broiler Chickens: Role of Dietary Supplementation of Ocimum sanctum, Ocimum basilicum with Organic Selenium V. Leela, L. S. S Varaprasad Reddy, G. Suganya, K. Loganathasamy, C. Devaraj 1

2. Biochemical Assessment of Allantoic Fluid during Different Stages of Pregnancy in Madras Red Sheep C. Devaraj, V. Leela 5

3. Biochemical Profile of Kilakarsal Ewes during Pregnancy, Post-partum, Lactation and Dry PeriodM. Sundara Vinayaki, V. S. Gomathy, C. Devaraj 10

4. Role of Pheromones and Biostimulation in Animal Reproduction-An OverviewR. P. Tiwari, Abrar Ahmed, G. K. Mishra 15

5. Treatment of Mange in Rabbits: A Case ReportAditi A. Dixit, M. L. V. Rao, G. Das, Kabita Roy, Virendra K. Thawait, Vijay Bhaskar U. 21

6. Torsion of Uterus as a Cause of Dystocia in a Nellore Brown EweK. Jyothi, M. Mutha Rao, Y. V. Pridhvidhar Reddy, A. Ganesan 24

7. Mortality Due to Pasteurellosis in CalvesSanjiv Kumar, S. Samantaray, D. Kumar, S. Kumari, R. Kapur 27

ContentsResearch & Reviews: Journal of Veterinary Science and Technology

RRJoVST (2014)© STM Journals 2014. All Rights Reserved

Research & Reviews: Journal of Veterinary Science and Technology ISSN: 2319-3441 (online), ISSN: 2349-3690 (print)

Volume 3, Issue 2

www.stmjournals.com

Antioxidant Enzyme Status in Broiler Chickens: Role

of Dietary Supplementation of Ocimum sanctum,

Ocimum basilicum with Organic Selenium

V. Leela, L. S. S Varaprasad Reddy, G. Suganya, K. Loganathasamy, C. Devaraj* Department of Veterinary Physiology, Madras Veterinary College,

Chennai, Tamilnadu, India

Abstract An experiment was conducted in broiler chicken to evaluate the effect of dietary

supplementation of Ocimum sanctum, Ocimum basilicum and selenium on antioxidative

enzyme levels. Total of forty two broiler chickens of day old divided into 6 groups of 7 each were used for this study. O. sanctum leaf powder (0.5%), O. basilicum leaf powder

(0.5%) and organic selenium (0.3 ppm) and their combinations were added to the basal diet. Superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and catalase levels

in plasma were measured at the end of 3rd

and 6th

week of age. Dietary supplementation

of selenium itself significantly (p<0.01) increased GSH-px activity and supplementation of both Ocimum species significantly increased SOD and catalase levels. However, O.

basilicum and O. sanctum in combination with selenium effectively enhanced the levels of

SOD, GSH-px and catalase. Between O. basilicum and O.sanctum with combination of selenium, no significant variations in antioxidant levels were seen. Both the plant sources

showed similar potency in scavenging free radicals. It is concluded that dietary supplementation of O.sanctum or O. basilicum at 0.5% level and its combination with

selenium (0.3 ppm) can combat oxidative stress in broilers, by enhancing antioxidative

enzyme levels.

Keywords: Antioxidants, Ocimum sanctum, Ocimum basilicum, Selenium, SOD,

GSH-px, Catalase, Broiler chickens



Volume 3, Issue 2

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Biochemical Assessment of Allantoic Fluid during

Different Stages of Pregnancy in Madras Red Sheep

C. Devaraj, V. Leela* Department of Veterinary Physiology

Madras Veterinary College, Chennai, Tamilnadu, India

Abstract To determine the biochemical constituents of sheep allantoic fluid during the different stages of pregnancy, sixty sheep uteri at various stages of pregnancy were collected from

the local slaughterhouse. The allantoic fluid samples were collected from each gravid

uterus. Foetal age was calculated according to crown anus length of the embryo/foetus by using the age estimation formula. At four stages of pregnancy viz., – I (30–60 days),

stage - II (61–90 days), stage - III (91–120 days) and stage - IV (121 to term), the

biochemical variables measured, respectively, were: glucose concentration were 8.13, 6.80, 4.30 and 3.21 mg/dl; total protein, 0.58, 0.62,0.79 and 1.03 g/dl; urea, 29.23,

36.03,51.68 and 67.06 mg/dl; creatinine, 5.98, 9.70,17.66 and 24.92 mg/dl, cholesterol, 3.08,3.32, 3.54 and 2.88 mg/dl; sodium, 94.20, 100.9, 89.00 and 97.86 mmol/L;

potassium, 4.40, 5.72, 4.26 and 5.50 mmol/L; calcium, 8.73, 8.67, 5.9 and 4.20 mg/dl and

magnesium, 0.32, 0.81, 0.22 and 0.01 mg/dl. Significant correlations (P<0.05) were found between gestational age and levels of glucose, total protein, urea, creatinine and

calcium. These findings might be helpful to understanding the foetal metabolism and

diagnose pathophysiological conditions during pregnancy.

Keywords: Allantoic, Biochemical assessment, Foetal metabolism, Pregnancy, Sheep



Volume 3, Issue 2

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Biochemical Profile of Kilakarsal Ewes during Pregnancy,

Post-partum, Lactation and Dry Period

M. Sundara Vinayaki*, V. S. Gomathy, C. Devaraj Madras Veterinary College, Chennai-600007, India

Abstract The present study was conducted to investigate the changes in biochemical parameters

during different physiological states of reproduction. The Kilakarsal sheep reared at the

Instructional Livestock Farm Complex, Tirunelveli, Tamilnadu, housed under standard management conditions in sheds with an open paddock was chosen for this study. Twenty-

four ewes were categorized into four groups of six each at different physiological phases

of late gestation, post-partum, lactation and dry period. The average live body weight of ewes in this study was 35 ± 1.62 kg. The study showed no significant difference in certain

biochemical parameters like magnesium (mg/dL), urea (mg/dL), creatinine (mg/dL) and non-esterified fatty acids (NEFA) during late gestation, postpartum, lactation and dry

period. However, significant (p < 0.05) differences were noted in certain biochemical

parameters like total protein (g/dL), calcium (mg/dL) and phosphorus (mg/dL) amongst the four groups of different stages.

Keywords: Biochemical parameters, Blood, Physiological status, Ewes



Volume 3, Issue 2

www.stmjournals.com

Role of Pheromones and Biostimulation in Animal

Reproduction-An Overview

R. P. Tiwari, Abrar Ahmed, G. K. Mishra* Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science

and Animal Husbandry, Anjora, Durg, (CG) India

Abstract In recent years, considerable progress has been achieved in the comprehension of profound effects of pheromones on reproductive physiology and behaviour. Pheromones

have been classified as molecules released by the individuals & responsible for elicitation

of specific behavioral expression in members of the same species. These signaling molecules, often chemically unrelated, are contained in body fluids of urine, sweat,

specialized exocrine glands and mucous secretion of genital. In mammals, signaling and

priming pheromones are thought to act either singly or in combination through olfaction, auditory, visual or tactile stimuli. Biostimulation or male effect is the stimulus provoked

by the presence of males, which induces estrus and ovulation through genital stimulation, pheromones or other external cues. The biostimulation technique offers a potentially

useful and practical way to improve reproductive efficiency in livestock species. The aim

of this manuscript is to examine the role of pheromone and biostimulation in animal reproduction that would provide information of interest for animal husbandry, where

economic circumstances do not allow the use of expensive and invasive techniques for

enhancing reproductive performances.

Keywords: Biostimulation, Neuroendocrine, Pheromones, Reproduction



Volume 3, Issue 2

www.stmjournals.com

Treatment of Mange in Rabbits: A Case Report

Aditi A. Dixit*, M. L. V. Rao, G. Das, Kabita Roy, Virendra K. Thawait, Vijay Bhaskar U. College of Veterinary Science & A.H., Durg (C.G.), India

Abstract A pair of rabbit was presented with the complaint of severe pruritis, alopecia,

inappetance and dullness. Skin scrapings revealed psoroptic mange infestation.

Treatment with ivermectin @ 200 µg/kg body weight along with antibiotics and liver extract preparation revealed clinical improvement in 14 days and complete recovery in

28 days.

Keywords: Mange, Ivermectin, Skin Scrapings, Parasitic Infestations

RRJoVST (2014) © STM Journals 2014. All Rights Reserved


Volume 3, Issue 2

www.stmjournals.com

Torsion of Uterus as a Cause of Dystocia in a Nellore

Brown Ewe

K. Jyothi*, M. Mutha Rao, Y. V. Pridhvidhar Reddy, A. Ganesan Department of Veterinary Gynaecology and Obstetrics,College of Veterinary Science,

Proddatur, Kadapa District-516360, Andhra Pradesh (India)

Abstract A Nellore brown ewe in its first parity was presented to the clinic with a complaint of dystocia. Pervaginal examination revealed right side post cervical uterine torsion.

Detortion of uterus was achieved by rolling the dam by modified schaffer’s method.

Torsion relieved but cervix was not dilated fully necessitating caesarean section but the owner preferred to dispose of the animal.

Keywords: Nellore brown ewe, Dystocia, Uterine torsion, Modified Schaffer’s method

RRJoVST (2014) © STM Journals 2014. All Rights Reserved


Volume 3, Issue 2

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Mortality Due to Pasteurellosis in Calves

Sanjiv Kumar1*, S. Samantaray

2, D. Kumar

1, S. Kumari

3, R. Kapur

4

1Department of Pathology, Bihar Veterinary College, Patna, Bihar, India

2Deptt. of Parasitology, BVC, Patna, Bihar, India

3Deptt. of Microbiology, BVC, Patna, Bihar, India

4Project Veterinary Officer

Abstract Mortality was recorded in fifteen Jersey calves in the cattle farm of Bihar Veterinary

College, Patna. All these calves before death were showing symptoms of high fever, severe dyspnoea, mouth breathing, profuse salivation and oedema in throat region. Dead

animals subjected to necropsy showed gross and histopathological lesions of

Haemmorhagic Septicemia. Stained heart blood smear and culture showed the presence of bipolar coccobacilli Pasteurella organisms. All the other ten ailing animals responded

well to the treatment and survived.

Keywords: Calves, Fibrinous exudates, Bipolar organisms, Haemorrhagic

Septicemia

Research & reviews journal of veterinary science and technology (vol3, issue2)

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