Reproductive cloning Biotechnology/Cloning. Reproductive cloning.
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Transcript of Reproductive cloning Biotechnology/Cloning. Reproductive cloning.
Reproductive cloning
Biotechnology/Cloning
Reproductive cloning
Therapeutic cloning
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Therapeutic cloning
Recombinant DNA technologyDNA isolated from
two sources and cut by the same enzyme
Gene of interest
RecombinantDNA
Plasmid(vector)
Transgenic organisms
Useful products
Gene for pestresistance
Gene fortoxic-cleanup
bacteria
Some uses of proteins:
Protein for“stone-washing”
jeans
Protein fordissolving
clots
Some uses of genes:
Restriction Enzymes
First Cloning Experiment
Cohen et al., 1973
“Recombinant DNA”
Cloning Vectors: pBR322 plasmid
Cloning into pBR322
RT-PCR and Cloning
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615
“Supermice”
Golden Rice
Weeding Out Land MinesFirst Glowing Pet