Regeneration in animal models
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Transcript of Regeneration in animal models
Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011
REGENERATION IN ANIMAL MODELS
Dr. Péter Balogh and Dr. Péter EngelmannTransdifferentiation and regenerative medicine – Lecture 3
Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat the University of Pécs and at the University of DebrecenIdentification number: TÁMOP-4.1.2-08/1/A-2009-0011
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Regeneration
Regeneration is the sequence of morphogenetic events that restores the normal structure of an organ after its partial or total loss/amputation. It exists at different levels in plants, invertebrate and vertebrate animals
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Physiological regeneration
Reparative regeneration
Hypertrophy
Morphallaxis
Types of regeneration in multicellular organisms
Tissue damage or loss
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• An ancient conglomerate of flagellate protists is developed.
• All cells at the surface shifted into subgroups of dividing cells and non-dividing cells. Those can be named as unipotent stem cells and non-stem cells.
• When multicellularity developed, there was a requirement for migrating stem cells to replace other cells inside the body.
Evolution of stem cells
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Regeneration in Porifera (sponges)• One cell population of sponges, the so called
archeocytes are active stem cells.• Archeocytes are able to differentiate into various
types of cells and repopulate themselves by self-renewal.
• Archeocytes give rise to choanocytes (participate in respiratory and digestive function), sclerocytes (important cells in innate immunity).
• Archeocytes also produce oocytes, while choanocytes produce sperm.
• In special occasions choanocytes transdifferentiate into archaeocytes.
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Regeneration in Hydra• Artificially dissected Hydra polyps can retain their
aggregation within 48 hours.• Individual animals do not increase their size since
growth is just balanced by loss of tissue in the form of buds in the lower gastric region and by sloughing of tissue at the ends of the tentacles and from the basal disk.
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Stem cell lineages in Hydra
• Epithelial cells in the Hydra body column continuously undergo mitotic divisions. Moreover, ectodermal and endodermal epithelial cells also exist.
• These two epithelial cell layers made up by stem cells. Hydra epithelial cells are capable, by successive divisions, both of indefinite self-renewal and of producing different types of specialized cells such as tentacle or foot specific epithelial cells.
• In addition, an interstitial stem cell layer is developed.
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Molecular factors of Hydra stem cells• Notch signaling• Wnt signaling• BMP molecules• JAK/STAT • Gene screen for stem cell related genes
(Sox2+, Nanog, Oct3/4??)
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Regeneration in planaria I• Planarians are bilaterally symmetrical animals found
in freshwater streams and ponds.• Planarians have the capacity to replace enormous
amount of missing regions through regeneration.• Planarian regeneration is referred to as morphallaxis.• Morphallaxis means cell proliferation / regeneration
events away from the wound tissue.
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Regeneration in planaria II Neoblasts• Planarian bidirectional regeneration is mediated by
neoblasts.• Approx. 30% of the total cells in the planaria are
neoblasts• Neoblasts can be found in the entire mesenchymal
region of the body with the exception of the pharyngeal region.
• Neoblasts divide by mitosis and can repopulate themselves. They are the only dividing cells in planaria.
• When a planaria is wounded, neoblasts migrate to the site and begin dividing.
• Neoblasts can become any cell type the planaria needs—nerve cells, reproductive cells, etc.
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Molecular pattern of neoblasts• Nanos RNA• Piwi RNA• Piwi subfamily - Argonaute proteins • miRNA• Wnt pathway• Shh pathway• FGF family
TÁMOP-4.1.2-08/1/A-2009-0011Regenerative capacity of axons inC. elegans• Axons are able to regrow in many animals after
injury except in mammals.• In C. elegans axonal regeneraton appears as early
as 4-5 hours following laser surgery, a growth cone-like structure is developed after 6-10 hours.
• DLK-1 pathway involved mainly in this regeneration process.
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Regeneration in annelids• It has been known for decades that annelids are
capable for efficient regeneration of their injured body parts.
• However the molecular aspects of this regeneration is more hidden.
• After 6-10 hours of the injury, neoblast cells are present and give rise to other tissue cells.
• Moreover, transdifferentiation of epithelial cells into nerve cells was observed.
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Regeneration in insects• Some insects are able to regenerate their legs and
other appendage organs.• Other insect species (flies) such as Drosophila do not
regenerate adult appendages, but from their imaginal discs they have a great regenerative behaviours during larval life.
• In this process several factors are participating such as decapentaplagic (dpp), wingless (wg) etc. molecules.
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Regeneration and colony fusion in protochordates• Protochordates share developmental history with
vertebrates at least through their early stages.• These colonial metazoans can give hints for
regeneration mediated by stem cell activity.• Colony development is dependent on self / non self
recognition mediated by a polymorphic gene family (Fu/HC) along with germ and somatic stem cell circulation.
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Regeneration of vertebratesThere are two types of regeneration:• Epimorphosis or epimorphic regeneration: This type
of regeneration involve the reconstruction of the missing parts by local proliferation from the blastema, or addition of parts to the remaining piece. For example: regeneration of tail, limbs and lens in amphibians and other vertebrates.
• Morphallaxis or morphallactic regeneration: This type of regeneration involving reorganization of the remaining part of the body of an animal. For example: Hydra, planaria and other invertebrates e.g. regeneration of the new individual from body pieces.
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Regeneration in fish IFin regeneration divided into four successive steps:1 Wound healing / closure within 3 hrs2 Blastema formation within 1 day3 Regenerative outgrowth concomitant to
differentiation within 2 days4 Patterning of blastema
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Regeneration in fish IIHeterogeneity of cell sourceDuring fin regeneration epidermis contains different compartments of blastema cells:• Distal• Proximal• Lateral
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Regeneration in fish IIImolecular patterns• Shh• Wnt• FGF• Activin b A• C-Jun, JunB
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Epimorphosis or epimorphic regenerationRegeneration of tail in amphibians and reptilia:Amphibia: The tail lacks vertebrae and has an unsegmented cartilaginous tube, which contains the regenerated spinal cord which form mainly of the ependymal lining of the central canal . At first very few cells accumulate under the wound epithelium . The ependyma and the various connective tissues dermis, muscle septa, adipose tissues and osteocytes of vertebrae are the sources of cells for the generate. The non-nervous elements proliferate behind the apex, forming both the muscle and cartilage tube ,then the ependyma proliferate and gradually extend dorsally.
Reptilia: For example lizard, the regenerated tail is a quite imperfect tail. It lacks vertebrae, and in their place, has an unsegmented cartilaginous tube. This tube contains the regenerated spinal cord, including the extension of the ependymal lining of the central canal of the spinal cord.
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Imprinting in regenerationAmputation
MA11A13
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InactivationOff
ActivationOnOn On
Never expressed
MemorizedRenewed
Gene expression
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Regeneration of the limbRegeneration begins in 3 phases:1 Phase of wound healing or pre -blastema stage:
Blood clotting and migration of epidermal cells from the basal layer of epidermis toward the centre of the wound. The wound is covered with epithelium which is thicker than the epidermis of the limb.
2 Phase of blastema formation:Cells accumulate beneath the epithelial coverings and form the blastema. Mesenchymal cells accumulate beneath the cap. Mesenchymal – blastemal cells differentiate into myoblasts and muscle cells, early cartilage cells and cartilage. During the dedifferentiation phase hyaluronate (HA) increases in the distal stump to form blastema . As the blastema forms, the HA concentration will be decreased. The production of HA and break down of collagen represent the establishment of migration from stump tissues.
3 Phase of dedifferentiation and morphogenesis:The blastema begins to restore the part of which the limb was deprived. Specifically, if the fore arm is removed, the blastema differentiated directly into the muscle, bone, cartilage and skin of the fore arm.
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Regeneration of amphibian lens 1 After removal or injury of the lens the dorsal region of the iris thickens
and a cleft arises between inner and outer lamellae of the iris.2 Amoeboid cells move from the stroma into the cleft followed by
marked increase of RNA and DNA synthesis as well as of mitotic cell division.
3 The pigmented cells of the dorsal region is engulfed by invading amoeboid cells.
4 The formed non- pigmented cubical cells form hollow epithelial vesicle and extends with inner and outer lamellae.
5 The vesicle inner wall cells elongated into the lumen and form primary lens fibers.
6 The lens-specific crystalline proteins are formed.7 The primary lens fibers push to the front of vesicle to form a nucleus
behind the lens epithelium which form the secondary lens fibers.8 The nucleus of primary lens fibers is enclosed by secondary lens fibers.9 In the central lens fibers the nucleus degenerate ,primary and
secondary lens fibers are the components of the lens.
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Neural stem cells differentiation capacity
Differentiation
NogginLow-RA FGF-2 FGF-2
Passage (6 days)
in vitro
in vivo
Somatic cells
Neurogenesis
Neurogenesis
Early neurogenesisProjecting neuron
Cholinergic neuronDopaminergic neuron
Motor neuron
Neuron
Gliogenesis
Gliogenesis
Late neurogenesisInterneuronGABAergic
neuron
NeuronAstrocyte
Oligodendrocyte
Differentiation
iPS cellsES cells
Embryoidbody
Primaryneurosphere
Embryos
Secondaryneurosphere
Neonatals Adults
Blastocysts
Blastocysts
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Factors controlling regeneration in vertebrates• Nervous system• Animal size• Pituitary gland• Vitamin A and its derivatives• Insulin
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Summary• All organisms possess a certain level of regeneration
capacity after tissue injury.
• At early stage of evolution, animals are able to regenerate whole body, however this capacity is more restricted to specialized tissues and organs in course of evolution.
• Neoblasts, hemoblast, progenitor, stem cells are participating in this process.