Reference Standards DNA & FFPE...
Transcript of Reference Standards DNA & FFPE...
Reference Standards
DNA & FFPE Samples
Mutation Cell Lines
CRIPSR-Cas9 for Footprint-Free Genome Editing
Homologous
Directed Repair
Custom Cell Line Engineering
Cell Type Modification Type
Cancel Cell Lines
KO
Point Mutation
Insertion
Human iPSCs
KO
Point Mutation
Insertion
Human ES Insertion
Primate iPSCs Insertion
Human Primary CellsKO
Insertion
Human Fibroblasts Point Mutation
Mouse Fibroblasts Point Mutation
Rat Thycrocytes KO
Selected lines from >100 distinct parental cell lines engineered:
A-549 BEAS-2B BT-474 HaCaT HBE Huh7 MCF-10A OCCM-30 RPE-1
SK-MEL-31 Tert-RPE U-2 OS 786-O CHLA-10 A-375 Gist-T1 DLD-1
HCT-116 HEK293 HEK293T HeLa HepG2 4T1 C2C12 cTEC ES Cells
iPSCs MWCL-1 BCWM-1 H929 Jurkat K562 KHYG-1 LAD2 MM.1s MWCL-1 NCI-H929 T2 cells TF-1 HT1080 HT29 KBM-7 KM12-Luc LnCaP MDA-MB231 NCI-H2228
RKO TC32 SCC35 SH-SY5Y
>400 Gene Edited Cell Lines Generated
Isogenic Cell Line – Engineering Workflow
• Clonally Homogeneous
Isogen
ic Pair
• Genetically Validated
• Homozygous Modification
Bio-Relevant Specimens
FFPE Cell Blocks
Tumor Xenografts
Amplicons
Plasmids
Genomic DNA
Engineering Clinically Relevant Mutations
Example 1: Point Mutation Example 2: Small Insertion Example 3: Deletion
150+ point mutations engineered across 15+ genes
KRAS EGFR BRAF NRAS HRAS PIK3CA IDH2 FGFR3 TP53 ESR1 FLT3 APC CTNNB1 PTEN BRCA1
Focused on MAPK Pathway
Exon 19 Mutation Panel
• 22 Deletion Mutations• In-frame mutations that occur within kinase domain• Target TKI’s therapeutics• 21 of 22 mutations are unique to ASC
114 Off-the-Shelf Mutations
Molecular Reference Standards
Assay Development • Optimize sample processing protocols• Establish limit of detection (LOD)• Identify and validate optimal reagents• Monitor the impact of workflow changes on results
Quality Control and Assay Reproducibility • Accurately evaluate batch-to-batch variability • Easily transfer workflows across labs or institutions
CE-Seq
NGS PCR
FISH
IHC
Reference Standards
Largest Cell Line Panel of EGFR Mutations
Molecular Reference Standards – FFPE Blocks and Slides
Intra-Block Inter-Block
Hematoxylin & Eosin Stained
Hematoxylin Stained
Apps
Molecular Reference Standards – FFPE Scrolls
Apps
• Simulate processing of biological specimens • Perfect for optimizing DNA extraction or
assay development
BRAF V600E TaqMan Assay
FFPE Scrolls
Tailored to your assay development needs
EGFR T790M mutant cell lines fixed and diluted into WT parental cells at targeted allelic frequencies of 50%, 10%, and 1% T790M
Accelerate Discovery Through Innovative Life Science