VENUCEANETMVENUCEANETM

Member of Croda International Plc

www.sederma.frE-mail: sederma@sederma.frCopyright© 2007-2013 Sederma. All rights reserved.

FACE MAKE-UP

Function:Anti-ageing.

Anti-UV+IR photo damage.(see previous results)

Definition:Biotechnological ferment containing multifunctional and stable enzymes.

Properties:VENUCEANE™ fights IR-induced

damage: lessens the inflammatory conditions, improves hydration and

maintains dermal architecture in order to limit wrinkle formation.

Characteristics:VENUCEANE™ prevents IR-induced

stress. In response to heat increase, VENUCEANE™ offers proportional

detoxifying activities and counteracts ROS production generated by IR.

Points of interest:Disturbed under IR radiation, the

mitochondrial integrity is protected by VENUCEANE™: energy production

and mitochondrial water content increase and contribute to hydration

improvement.

INCI Name:Thermus Thermophilus Ferment –

Glycerin

Applications:Protective day creams, sun protection

products, make-up emulsions.

Formulation:Water soluble. Incorporate at room

temperature or at 45°C in an emulsion.

Recommended use level:1.5-5%

Patent:WO 02/066668

EP 1 392 840FR 2 821 086

Hydrothermal vents

Red card for InfraRed

IR-irradiated control Venuceane™ 1.5%

The new generation of anti-ageing

photo-protection

Protection of dermal fibres

Collagen I +70% p<0.01

UVA HEV IR-A IR-B IR-C

dermis

hypodermis

epidermis

Sederma 29 rue du Chemin Vert 78612 Le Perray-en-Yvelines cedex France Tel. : +33 (0)1 34 84 10 10 Fax : +33 (0)1 34 84 11 30

Non-guarantee: The information in this publication is given in good faith by Sederma by way of general guidance and for reference purposes only. The information should not be construed as granting a license to practice any methods or compositions of matter covered by patents. Sederma assumes no liability in relation to the information and gives no warranty regarding the suitability of the product and/or ingredients described for a particular use. The use made of the product and/or ingredients described by the recipient and any claims or representations made by the recipient in respect of such product and/or ingredients are the responsibility of the recipient. The recipient is solely responsible for ensuring that products marketed to consumers comply with all relevant laws and regulations.

v. 130731

Reproduction of all or part of this document without the express prior written consent of Sederma is strictly prohibited.

VENUCEANE™ FIGHTS IR-AGEING

INFRARED RADIATION AND SKIN AGEING

VENUCEANE™ PREVENTS IR-INDUCED STRESS

CLAIM SUBSTANTIATION 06-2013

ADAPTIVE RESPONSE TO HEAT INCREASE COUNTERACTION OF IR-INDUCED ROS PRODUCTION

HYDRATION IMPROVEMENT DERMAL ARCHITECTURE MAINTENANCE

Thanks to detoxifying activities proportional to heat increase, VENUCEANE™ counteracts IR-induced ROS production and oxidative stress propagation.

By preserving the mitochondrial integrity, Venuceane™ stimulates the mitochondrial metabolism.

Venuceane™ protects the dermal fibres from IR radiation.

Detoxifying activities according to temperature, Venuceane™ eq 3%Increase in enzymatic activity from 25°C to 40°C

SOD (superoxide dismutase-like) .................................................. x1.4 Catalase-like ............................................................................... x3.0 GPO (glutathione peroxidase-like) ................................................. x2.8

On human keratinocytes irradiated with IR ROS production .........................+29%, p<0.01 vs non-irradiated control ROS production with Venuceane™ eq 1.5% ......... -28%, p<0.01 vs IR-irradiated

control

On human keratinocytes, Venuceane™ eq 1.5% ATP synthesis and mitochondrial water content ........... +123%, p<0.01

Collagen integrity

Elastin architecture

On skin explants (for MMP-1) and on human fibroblasts irradiated with IR.

PROTECTION FROM INFLAMMATIONOn human fibroblasts irradiated with IR Pro-inflammatory mediators production (pg/106 cell.)

PGE2 IL-6 IL-8

Irradiated vs non-irradiated +31% p<0.05

+27% p<0.01

+18% p<0.05

Venuceane™ eq 1.5% vs IR-irradiated control

-54% p<0.01

-53% p<0.01

-61% p<0.01

MMP-1 HSP-47Collagen I

collagen destruction collagen synthesis

Irradiated vs non-irradiated +333% p<0.01

-36% -79%p<0.01

Venuceane™ eq 1.5% vs IR-irradiated control

-38% p<0.01

+44% +70%p<0.01

IR-irradiated control

Venuceane™ 1.5%

Formulation

Part A .................................................................................................%Water deionised (Aqua) qs 100ViscOptima PL (Sodium Polyacrylate (and) Paraffinum Liquidum (and) Trideceth-6) 0.35

Part B .................................................................................................%Cyclopentasiloxane (and) Cyclohexasiloxane 2.00Crodamol AB-LQ-(RB) (C12-15 Alkyl Benzoate) 4.50Cromollient DP3A-LQ-(MH) (Di-PPG-3 Myristyl Ether Adipate) 1.00Crodamol GTEH-LQ-(MV) (Triethylhexanoin) 2.00Crodamol CSO-LQ-(JP) (Ceteraryl Ethylhexanoate) 3.00Optasense G82 (Acrylates/C10-30 Alkyl Acrylate Crosspolymer) 0.15

Part C .................................................................................................%Glycerin 5.00Preservatives [•] qs

Part D .................................................................................................%Potassium sorbate 0.10

Part E .................................................................................................%Water deionised 1.30Sodium hydroxide 30% 0.13

Part F .................................................................................................%Venuceane™ (Thermus Thermophilus Ferment (and) Glycerin) 3.00

Part G .................................................................................................%Fragrance (Orchid) 0.10

Protocol: Disperse the ViscOptima PL in the water with Staro stirring (s=1000 rpm). Weigh and mix Part B. Weigh and mix Part C. Weigh Part D. Add Part B to Part A with strong Staro stirring (s=2700 rpm). Extemporaneously, add Part C, then Part D to Part A+C. Neutralise with Part E and allow stirring with Staro (s=1000) for 30 minutes. Add Part F, homogenise well. Add Part G, homogenise well. Check pH at 6.00 +/- 0.10.

[•] The addition of Osmocide™4 allows to reduce the concentration of traditional preservatives

Non-guarantee:This formulation has been subjected to limited stability tests and has been shown to perform well. However formulators adopting this approach should ensure to their own satisfaction long term stability and functionality. It is good practice to conduct safety tests on all final formulations prior to marketing. Suggested uses should not be taken as an inducement to infringe any existing patents.

UVB

Infrared radiation (IR) represents more than 50% of solar radiation, it generates a heat increase, penetrates deep into the skin and appears to have non-negligible effects on skin physiology. UVB UVA HEV* IR-A IR-B IR-C

290-320 320-400 380-500 750-1400 1400-3000 3000-1mm

ATPADP+ Pi

H+H+H+

H+

e-e-

e-e-

QIIII IV

VO2

H2OO2H2O

ADP + Pi + H+ = ATP1/2 O2 + 2H++ 2e-= H2O

mitochondria mitochondrial respiratory chain

Anti-ageing Infrared Protection Serum with Venuceane™ Indicative formula Ref.: SED 1111981 G

Fibrillin-1

microfibril contituent

Irradiated vs non-irradiated -17%p<0.01

Venuceane™ eq 1.5% vs IR-irradiated control

+28%p<0.01

* (High Energy Visible)

Wavelength Range (nm)