Recombinant vaccines
description
Transcript of Recombinant vaccines
WEL-COME
PRESENTATION ON
“RECOMBINANTVACCINES”
PRESENTED BY - AAYASHA HASANB.Sc IISUPERVISED BY-
Dr. J.B. CHAWLA
WHO Top Priorities
PLAGUE
TUBERCULOSIS
TYPHUS
CHOLERA
INFLUENZA
YELLOW FEVER
MALARIA
PANDEMICS AND
NOTABLE EPIDEMICS THROUGH HISTORY
PLAGUE Europe's population dropped by around 50% between 550 and 700 A.D.
TYPHUS During World War I, typhus epidemics killed over 150,000 in Serbia.
SMALLPOX Killed around 400,000 Europeans per year during the end of the 18th century.
MALARIA Each year, there are approximately 350–500 million cases of malaria.
TUBERCULOSIS Annually, 8 million people become ill with tuberculosis, and 2 million people die from the disease worldwide.
Cholera epidemic
More than 20,000 pilgrims died of cholera during the
1907–08 in Mecca.
Influenza epidemicOctober 1918, Influenza virus infected about one-third of the
world‘s population.
METHODS TO CONTROL THE DISEASES
Destruction of the source
Blocking the modes of transmission
Protecting the susceptible host
Most effective way is to produce
vaccination
Any antigenic preparation which stimulates host immune system to form antibodies or T-Cells against it and which will protect the host against future infections is known as vaccines.
Cost effective Effective after a single doseApplicable to number of diseasesAdministered by a oral routeCould be given early in life
1ST GENERATION VACCINEWhole cells/virus particles.
E.g.:-B.C.G; polio drops etc.
2ND GENERATION VACCINE Antigenic components of the organism.
E.g.:-Tetanus toxoid;Hepatitis B virus vaccine; Pneumococcal vaccine(contains capsular polysaccharide).
3RD GENERATION VACCINEPlasmids/genes responsible for the antigen formation.
E.g.:-DNA Vaccine.
3 CATEGORIES OF VACCINES
DRAWBACKS OF TRADITIONAL VACCINES
Not possible for organisms which are difficult to cultivate.
Yield of vaccine is low.
Large number of antigens stimulate immune system which is not desirable.
Danger of reverting back to pathogenic state.
SUBUNIT VACCINE
DNA VACCINE
CONJUGATE VACCINE
EDIBLE VACCINE
Recombinant vaccines is having the potential to give safe and more effective vaccines
TYPES
These vaccines contain proteins or peptides from pathogenic organisms prepared by recombinant DNA technology.
CONJUGATE VACCINEThe vaccine is prepared by conjugating the surface polysaccharides of Hib to a protein molecule.
Viral geneExpression
plasmid
Plasmid with foreign gene
DNA vaccines
Bacterial cell
Plasmid with foreign gene introduced into a suitable bacterial cell
Plasmid DNA gets Amplified
Plasmid DNA carrying Viral gene Purified from the bacterial cells
DNA VACCINE
The gene from the pathogen(viral gene) gets expressed by the host cells and antigen molecules formed stimulate both cellular and humoral immunity in the host.
Plasmid DNA with viral gene
Antigenic Protein
DNA vaccines induce both humoral and cell mediated immunity.
DNA vaccines cause prolonged expression of the antigens,thus leads to longer protection.
Refrigeration of DNA vaccine is not required which greatly lowers the cost and reduces complexity of delivery.
Advantages of the DNA vaccines
Only proteins antigens can be encodedVaccines where polysaccharide antigens are to
be used (such as in pneumococcal or meningococcal vaccines)
could not be made.
Limitations of the DNA vaccines
Present Scenerio
Human trials are underway with several DNA vaccines
AIDSInfluenza
EbolaHerpes virus
Malaria
A vaccine in which an antigenic protein is
engineered into an edible plant;
after ingestion, the protein stimulates the
immune system of the host.
EDIBLE VACCINE
Edible vaccines are currently being studied in
plants such as potato, tomato, banana, corn, lupine, lettuce
and others so that children could simply eat a vegetable
or fruit and have immunity to a disease.
Shoud I take Injection Or
Should I Eat Banana?
CHOICE IS YOURS
Recombinant vaccines are in pipeline for
Cancer Filaria
DiarrhoeaAIDS
InfluenzaEbola
HerpesMalaria
Near Future possibilities
THANKS TO ALL!!!
THE END….ANY QUERIES… … … …