Recombinant DNA Bacterial Transformation Teacher Preparation Sterile Materials
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Transcript of Recombinant DNA Bacterial Transformation Teacher Preparation Sterile Materials
Recombinant DNA Bacterial Transformation
Teacher Preparation
Sterile Materials
Recombinant TransformationsSterilized Materials List
(per group of students)
1 pack of 8-10 toothpicks wrapped in aluminum foil
1 pack of 4 large paper clips bent at 90o wrapped in aluminum foil
50 ml Sterile Calcium Chloride solution
50 ml Sterile Water
Recombinant Transformation Sterile Materials(per group of students)
• 1 aluminum foil packet containing 8-10 toothpicks
Recombinant Transformation Sterile Materials(per group of students)
• 1 aluminum foil packet containing 4 large, smooth paper clips. The clips should be opened into a 90° angle and the small ends bent up.
Recombinant TransformationSterile Materials
• Heat the foil packages containing paper clips and toothpicks at 350oF for at least 30 minutes to sterilize.
Recombinant TransformationCaCl2 Solution
• Dissolve 0.75 g of CaCl2 into 50 ml of distilled water in a labeled 100-ml glass bottle with a cap.
Recombinant Transformation CaCl2 Solution
• Cap the bottle and place it in boiling water for 30 minutes. Cap should be "snugged" down on the bottle.
• Refrigerate to prevent contamination.
Recombinant Transformation Sterile Distilled Water
• In a bottle with a cap, add 50 ml of distilled water.
• Cap the bottle and place it in boiling water for 30 minutes. Cap should be "snugged" down on the bottle.
• Refrigerate to prevent contamination.