Reaqua BT99

1.Reaqua BT99 Reaqua BT99 is a betaine,betaine is discovered by the Scheibler, an

d separated from the sugar beet, It is a completely natural, edible am

ino acids. Since 1939, DU Vigneaud found its nutritional function,it

has become a hot topic of research, and is widely used in the pharma

ceutical, food, additives, chemical reagents, chemical raw materials,

livestock breeding, aquaculture and other fields.

❀ White crystalline powder❀ Sweet and bitter taste❀ Soluble in water, ethanol, PG and glycerin❀ Solubility:

160g Reaqua BT99 / 100g water8,7g Reaqua BT99 / 100g ethanolslightly soluble in ether

❀ Compatible with anionic, cationic and nonionic materials❀High temperature resistant❀Heated to 310°decomposition❀Loss on drying(105℃, 2 hours) ≤1.0%❀Reaqua BT99 Content ≥99.0%❀Residue on ignition ≤0.1%❀PH Value (5%) 5.0-7.0❀Sulphate ≤20 ppm❀Heavy metal(Pb) < 10 ppm❀Arsenic < 2 ppm

The Physical properties

of Reaqua BT99

CH3

CH3

CH3

N+

CH2

COO-

. H O2

Chemical structure ：

2.The function of Reaqua BT99

2.1 Reaqua BT99 is a major osmolyte

K+

Sugars

Amino Acids

Reaqua BT99

H2O

• Water follows osmotically active compounds such as potassium and organic osmolytes

• Water doesn`t move alone

Disturbance

Original cell volume

Swell

Shrink

Original cell volume

Recovery

Osmolyte efflux

Osmolyte uptake

Cells regulate their volume and water balance by absorbing or effluxing osmolytes

2.2 Maintain moisture balance• Water is common base to all biological solutions and thus to life itself• All cells are 85-95% water

Reaqua BT99 attracts water but does not immobilize it as a moisturizer

2.3 Solubilization of insoluble material, such as salicylic acid and allantoin• Salicylic acid

– Use levels from 2 to 10 %.– Solubility in water is 0.2 % at room

temperature – Reaqua BT99 was found to increase the

solubility of salicylic acid to 5 %

• Allantoin -solubility in water 0.7 % at RT

-Reaqua BT99 was found to increase the solubility of allantoin

to 2 %

2.4 Buffering properties of Reaqua BT99

pH values after addition of HCl 1N to 100 mls of 1M Reaqua BT99 aqueous solution

0

1

2

3

4

5

6

7

0 2 4 6 8 10 12

HCl 1N (ml)

pH

Betaine 1M Water

2.5 Polymers swelling

• Viscosity values during the swelling phase of 1% water and water- Reaqua BT99 dispersions of Sodium• carboxymethylcellulose

0,14% Na CARBOMER aq

2.6 Reaqua BT99 ability to reduce the irritating effects of detergents

0

0,5

1

1,5

2

2,5

3

3,5

4

4,5

5

0 2 3,5 5 7

Betaine(%)

Irrita

tion

Scor

e

24 h

48 h

Human patch test SLES/Reaqua BT99 on 25 volunteers

1. Test solution: 10.3 % SLES 0 - 7 % Reaqua BT992. 25 µL of the test solution was applied on the forearm in a „Finn chamber“(sealed Al-chamber)3. Removal of the Finn chamber after 24 and 48h, respectively.4. Dermatologists assessed skin irritation 1 hour after removal

0

200

400

600

800

SLES mix SLES/DLSS mix+3,5 Betaine Betaine

Irritant

Slightly Irritant

Non irritant

Increased Reaqua BT99, Inositol, & Taurine uptakes

(Warskulat et al. 2004)

InositolReaqua BT99

Taurine

Oxidative Stress Opening of K+ channels Cell shrinkageIncreased Reaqua BT99, Inositol and Taurine transporters & uptake

2.7 Osmotic Stress : UV radiation

2.8 Sensory properties

Reaqua BT99 modifies the feelof aq. Solutions and emulsions with 3-4 %

❀ Glycerol❀ Sorbitol❀ Propylene glycol

Best performer❀ 50/50 glycerol/ Reaqua BT99 3-4 %

2.9 Improving the performance of foamFoam texture when 0% Reaqua BT99 included

Foam texture when 5% Reaqua BT99 included

Thank you ！