ReaMetrix Hiv Reagent Portfolio

© 2008 Reametrix, Inc. All rights reserved.

Management of HIV Infection in Resource Poor Settings

Accurate & Affordable Reagent Alternatives

2© 2008 Reametrix, Inc. All rights reserved.

HIV in India – Current Status

HIV Prevalence rate in India 0.36% 2.5 Million HIV Infections

By year 2010, estimates indicate that there will be between 8-15 million HIV infected individuals

Management of HIV InfectionProviding Anti Retroviral therapy (ART)Immunophenotyping

CD4 T-Lymphocyte Count

Viral Load Assay – PCR – quantitate virus levels in blood

CD4 counts are the single most important criteria to guide clinical decision-making on

when to use ART


ReaMetrix Solutions for CD4 EnumerationDry-Tri T-STAT CD3483 color single tube reagent for open flow Cytometers eg. BD FACScan ™, BD FACSCalibur ™, BC EPICS XL™)

Dry-Tri T-STAT CD34453 color single tube reagent for open flow Cytometers(eg. BD FACScan™, BD FACSCalibur™, BC EPICS XL™)

ReaPan 348454 color single tube reagent for open Flow Cytometers(eg. BD FACSCalibur™)

Rea T count2 color dual tube reagent for FACScount™ Platform

ReaPan 34G-38G2 Color dual tube reagent for the Guava™ Platform


ReaMetrix Assay Reagent Characteristics Methodology

Absolute CD4 testing by flow Cytometry – gold standard method

Employ simple Lyse/no wash protocol - single platform technology

Convenience Provided as a ready to use format. No additional dispensing

Unitized formPre-dispensed reagents in unitized form – reduces pipetting

errors

Stability & storageDried down reagents – do not require cold storage. No

aggregation of antibodies & beads in dried form

Simplified work flowMinimal hand on time - just require sample pipetting, reduced

steps


ReaMetrix Assay Reagent Characteristics

Accurate & reproducibleWorkshop approved antibody clones – provide excellent

Analytical Specificity & Analytical Sensitivity Extensively validated reagents in various private & public labs

in India

Sample stabilityProcessed samples can be stored for up to 7 days prior to analysis (CV = 5.7%).

AffordableAll of these attributes without compromising affordability

Technical supportReaMetrix provides training on Cytometer and reagentReaMetrix provides technical support online & through personal

visits


Reagents are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Beckman Coulter EPICS-XL etc.

Reagents supplied in Dry, Unitized Format

Dry-Tri T-STAT CD348

3 color single tube reagentAnti CD4 – Atto488* (FITC eq. dye)Anti CD8 – R-PEAnti CD3 – R-PE-Dy649* (Cy5 eq. dye)

Provides absolute counts (Cells/µL) of CD3+, CD4+, CD8+ Cells & CD4/CD8 Ratio Validated with commercial reagents at NARI, YRG CareCE Marked reagent

* Dyes qualified by ReaMetrix to assure superior performance


Reagents are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Beckman Coulter EPICS-XL etc.


Dry-Tri T-STAT CD3445

3 color single tube reagentAnti CD4 – Atto488* (FITC eq. dye)Anti CD3 – R-PE-Dy649* (Cy5 eq. dye)Anti CD45 – R-PE

Provides absolute counts (Cells/µL) of Absolute Lymphocyte Count (ALC), CD3+, CD4+, % CD4 CountsValidated with commercial reagents at leading path labs in IndiaSuited for aged blood samples

* Dyes qualified by ReaMetrix to assure superior performance



ReaPan 34845

44 color single tube reagent, Anti CD4 – APC Anti CD8 – R-PEAnti CD3 – Atto488* (FITC eq. dye)Anti CD45 – PerCP

Provides absolute counts (Cells/µL) of ALC, CD3+, CD4+, CD8+ Cells, % CD4 & CD4/CD8 Ratio Validated with BD multiTEST 4 color reagent at leading path. Lab in India.Suited for aged blood samplesDesigned for BD FACSCalibur dual laser flow CytometerCE marked * Dyes qualified by ReaMetrix to assure superior

performance



Rea T-Count Reagent

Rea T-count CD3/CD4 & CD3/CD8 reagentagentDesigned for BD FACSCount flow CytometerAvailable as 2 color dual tube reagent for

CD3+, CD4+, CD8+, CD4/CD8 ratio estimation

Available as 2 color single tube reagent for

CD3+, CD4+ estimation

Alternative for FACSCount reagent Reagent validated at

National HIV Immunology laboratory, CanadaNational HIV Immunology laboratory, Canada

ZimbabweZimbabwe


Reagents supplied in dry, unitized format

ReaPan Reagents for the Guava Platform

ReaPan 34G2 color single tube reagent Provides absolute counts (Cells/µL) of CD3+, CD3+CD4+ counts

ReaPan 34G & 38G2 color single dual reagentProvides absolute counts (Cells/µL) of CD3+, CD3+CD4+ & CD3+CD8+ counts

Validated with Guava Reagent at KIMS, Bangalore


Summary…

ReaMetrix supplies all reagents with ReaLyse, a buffer for lysing and fixing samples or ReaFix, a buffer for fixing samples after staining

ReaMetrix has the capability to develop CD4+ enumeration solutions for any flow cytometer platform.

ReaMetrix can customize solutions as per your need.

Work is in progress for reagent development for other platforms.


Manufactured by ReaMetrix India Pvt. Ltd.50-B, II Phase, Peenya Industrial Area

Peenya, Bangalore 560058, IndiaPh: +91-80-28378693/5, Fax: +91-80-

41172451E-mail: [email protected]

www.reametrix.com

Further details:

ReaMetrix Hiv Reagent Portfolio

Health & Medicine

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