REAL-TIME PCR KITS FOR DIAGNOSIS - cdn.aumet.me · Real Time PCR (qPCR) has revolutionized...

REAL-TIME PCR

KITS FOR DIAGNOSIS0483

Reproducible, Sensitive and Precise Analysis

Real Time PCR (qPCR) has revolutionized biological and medical sciences by providing quantitative detection of specific nucleic acid sequences. Today, Real Time PCR is one of the primary choices of the clinicians and labora-tory professionals for the diagnosis of the pathological diseases.

Efficient extraction of the viral nucleic acid from the sample, separately detected internal controls, specifically designed primers and probes, WHO calibrated external standards and consequently; reliable amplification sys-tem and analysis are all the critical parameters to get the confidential results for the detection of the specific viral nucleic acid sequences.

RTA Real Time PCR Kits are the outcome of an in-depth research. They are designed for the detection of HIV-1, HBV, HCV and CMV nucleic acids in serum or plasma samples with highly accurate quantification. For your re-producible, sensitive and precise analysis; we offer you to benefit from the experience and knowledge we put in our products.

Certification

In Vitro Diagnostic Medical Devices Directive (98/79/EC) is a CE marking directive comprising the regulatory requirements that must be followed by in vitro diagnostic medical device manufacturers. The aim of the direc-tive is to ensure that only safe and functional products meeting the identical quality and purity requirements are sold in diagnostic market.

The quality and the compliance of RTA Real-Time PCR Kits to this directive is certified and marked with CE-IVD by “MDC Medical Device Certification” Notified Body (0483) in Stuttgart/Germany together with the confirma-tion of Paul Ehrlich Institute of Germany.

HEPATITISBHepatitis B Virus DNA Detection

RTA HBV Real-Time PCR Kit is an in vitro nucleic acid amplificationassay for the quantitation of Hepatitis B Virus RNA in human serumor plasma samples. The kit is designed to be used as an aid in the managementof patients with chronic HBV infection undergoing anti-viral therapy to assess response to treatment in conjunction with all relevant clinical and laboratory finding.

RTA HBV Real-Time PCR Kit provides you everything needed for a reliable and efficient detection:

• Very high sensitivity of detection (as few as 11.8 IU/ml) • Extended linear range • Complete specificity for all eight HBV genotypes • Whole process check by adding the internal control during extraction • 4 external quantitation standards calibrated against the WHO standards • Compatibility with widely used thermal cyclers • 98/79/EC EU IVD Directive compliance

RTA HBV Real-Time PCR Kit is perfectly designed to amplify and detect a highly conserved region within the HBV genome by Real Time PCR. It is possible to monitor the nucleic acid amount on-line, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the DNA amount.

Sample Preparation: Nucleic acid extraction is a decisive step prior to qPCR since; providing high yields of nucleic acid and removal of PCR inhibitors in turn improve the amplification efficiency and critical for avoiding false negative PCR results.

RTA HBV Real-Time PCR Kit has been validated for use with RTA Viral DNA Isolation Kit or RTA ViralNucleic Acid Isolation Kit for its best performance.

Analytical sensitivity

Specificity

Genotype detection

Linear range

Compatible RTPCR Instruments

11.8 IU/ml

%100

All eight HBV genotypes

21 IU/ml – 1x109 IU/ml

Inceptra 4840/9640/9660Stratagene Mx3000p/Mx3005pLight Cycler 1.5/2.0BioRad CFX96

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NCQS 4QS 3QS 2QS 1

Figure 1.Detection of the quantitationstandards inBioRad CFX96.NC: NegativeControl.

HEPATITISCHepatitis C Virus RNA Detection

RTA HCV Real-Time PCR Kit is an in vitro nucleic acid amplificationassay for the quantitation of Hepatitis C Virus RNA in human serumor plasma samples. The kit is designed to be used as an aid in the managementof patients with chronic HCV infection undergoing anti-viral therapy to assess response to treatment in conjunction with all relevant clinical and laboratory finding.

RTA HCV Real-Time PCR Kit provides you everything needed for a reliable and efficient detection:

• Very high sensitivity of detection (as few as 9.5 IU/ml) • Extended linear range • Complete specificity for all seven HCV genotypes • One-step real time reverse transcription PCR • Whole process check by adding the internal control during extraction • 4 external quantitation standards calibrated against the WHO standards • Compatibility with widely used thermal cyclers • 98/79/EC EU IVD Directive compliance

RTA HCV Real-Time PCR Kit is perfectly designed to amplify and detect a highly conserved region within the HCV genome by Real Time PCR. It is possible to monitor the nucleic acid amount on-line, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the RNA amount.


RTA HCV Real-Time PCR Kit has been validated for use with RTA Viral RNA Isolation Kit or RTA Viral Nucle-ic Acid Isolation Kit for its best performance.

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10 20 30 40 50

NCQS 4QS 3QS 2QS 1



Specificity

Genotype detection

Linear range


9.5 IU/ml

%100

All seven HCV genotypes

9.5 IU/ml – 1x109 IU/ml


HIV-1Human Immunodeficiency Virus-1 RNA Detection

RTA HIV-1 Real-Time PCR Kit is an in vitro nucleic acid amplificationassay for the quantitation of Human Immunodeficiency Virus RNA inhuman serum or plasma samples. The kit is designed to be used as an aid in themanagement of patients with chronic HIV-1 infection undergoing anti-viral therapy toassess response to treatment in conjunction with all relevant clinical and laboratory finding.

RTA HIV-1 Real-Time PCR Kit provides you everything needed for a reliable and efficient detection:

• Very high sensitivity of detection (as few as 85 IU/ml) • Extended linear range • Complete specificity for all major nine HIV-1 genotypes • One-step real time reverse transcription PCR • Whole process check by adding the internal control during extraction • 4 external quantitation standards calibrated against the WHO standards • Compatibility with widely used thermal cyclers • 98/79/EC EU IVD Directive compliance

RTA HIV-1 Real-Time PCR Kit is perfectly designed to amplify and detect a highly conserved region within the HIV-1 genome by Real Time PCR. It is possible to monitor the nucleic acid amount on-line, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the RNA amount.


RTA HIV-1 Real-Time PCR Kit has been validated for use with RTA Viral Nucleic Acid Isolation Kit for its best performance.

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Cycle number

0.4

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10 20 30 40 50

NCQS 4QS 3QS 2QS 1



Specificity

Genotype detection

Linear range


85 IU/ml

%100

All nine HIV-1 genotypes



CMVCytomegalovirus DNA Detection

RTA CMV Real-Time PCR Kit is an in vitro nucleic acid amplificationassay for the quantitation of Cytomegalovirus RNA in human serumor plasma samples. The kit is designed to be used as an aid in the managementof patients with chronic CMV infection undergoing anti-viral therapy to assess response to treatment in conjunction with all relevant clinical and laboratory finding.

RTA CMV Real-Time PCR Kit provides you everything needed for a reliable and efficient detection:

• Very high sensitivity of detection (as few as 103 IU/ml) • Extended linear range • Complete specificity for All relevant genotypes and subtypes • Whole process check by adding the internal control during extraction • 4 external quantitation standards calibrated against the WHO standards • Compatibility with widely used thermal cyclers • 98/79/EC EU IVD Directive compliance

RTA CMV Real-Time PCR Kit is perfectly designed to amplify and detect a highly conserved region within the CMV genome by Real Time PCR. It is possible to monitor the nucleic acid amount on-line, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the DNA amount.


RTA CMV Real-Time PCR Kit has been validated for use with RTA Viral DNA Isolation Kit or RTA Viral Nucleic Acid Isolation Kit for its best performance.

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0

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Flou

resc

ence

Cycle number

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10 20 30 40 50

NCQS 4QS 3QS 2QS 1



Specificity

Genotype detection

Linear range


103 IU/ml

%100

All relevant genotypes and subtypes



MIXMASTER

Ordering List

HBV DNA Real Time PCR Kit

Test: 25 Catalog#: 09011025

Catalog#: 09011100Test: 100

HCV RNA Real Time PCR Kit



DNA QPCR Master Mix Kit


Catalog#: 09030100

Catalog#: 09030500

Test: 100

Test: 500

HIV-I RNA Real Time PCR Kit



CMV Real Time PCR Kit



DNA qPCR Probe Master Mix

RTA DNA qPCR Master Mix Kit is a ready-to-use, master mix that contains all thereagents (except primers, probes and templates) needed for running quantitative,real-time DNA detection assays.

•Convenientuse-minimizingpipettingsteps•Highsensitivityofdetection•Highstabilityandefficiency–minimumvariationbetweenresults

RTA DNA qPCR Master Mix Kit is also convenient for several purposes including the analyzing of gene expression, microbial detection and the determination of viral load.

Rta Laboratories

Plastikçiler OSB (GEPOSB)Cumhuriyet Cad. No:3 41400Gebze / Kocaeli - Turkey

T: +90 262 648 53 00F: +90 262 751 06 77

[email protected]

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REAL-TIME PCR KITS FOR DIAGNOSIS - cdn.aumet.me · Real Time PCR (qPCR) has revolutionized...

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