Reagent #2 0.001M luminol in 0.1M NaOH pH 12 , volume 100 µL
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Reagent #2 0.001M luminol in 0.1M NaOH pH 12, volume 100 µL Carrier: an aqueous solution containing 0.025% (v/v) Brij Sample Fe (II) in 0.001 N HCl volume 50 µL Chemiluminescence: STEP#1 REAGENT LOADED INTO FLOWCELL STEP#2 SAMPLE INJECTED INTO FLOWCELL Sequencing of Sample and Reagents Hugo Oliveira, unpublished results from N.B. Palmer cruise Antarctica 2011 y = 14.798x + 116.24 R 2 = 0.9997 0 200 400 600 800 1,000 1,200 1,400 1,600 1,800 2,000 0.0 20.0 40.0 60.0 80.0 100.0 120.0 140 Fe /nM PeakHeight counts FIBER OPTICS Flow Cell Holding Coil LUMINOL Fe PRODUCT
description
Chemiluminescence :. Sequencing of Sample and Reagents. Holding Coil. Flow Cell. counts. LUMINOL. FIBER OPTICS. Fe. PRODUCT. STEP#1 REAGENT LOADED INTO FLOWCELL STEP#2 SAMPLE INJECTED INTO FLOWCELL. Reagent #2 0.001M luminol in 0.1M NaOH pH 12 , volume 100 µL - PowerPoint PPT Presentation
Transcript of Reagent #2 0.001M luminol in 0.1M NaOH pH 12 , volume 100 µL
Reagent #2 0.001M luminol in 0.1M NaOH pH 12,volume 100 µL Carrier: an aqueous solution containing 0.025% (v/v) Brij Sample Fe (II) in 0.001 N HCl volume 50 µL
Chemiluminescence:
STEP#1 REAGENT LOADED INTO FLOWCELL STEP#2 SAMPLE INJECTED INTO FLOWCELL
Sequencing of Sample and Reagents
Hugo Oliveira, unpublished results from N.B. Palmer cruise Antarctica 2011
y = 14.798x + 116.24
R2 = 0.9997
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Fe / nM
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FIBER OPTICS
Flow CellHolding Coil
LUMINOL
Fe
PRODUCT