Raman Spectroscopy lab - fisicaechimica.unipa.it · raman spectroscopy lab ... manual xyz...
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TECNOLOGIA RAMAN TECNOLOGIA RAMAN Simonpietro Agnello Raman Spectroscopy lab 28/09/2016 Department of Physics and Chemistry – University of Palermo https://fisicaechimica.unipa.it/agnello/
Transcript of Raman Spectroscopy lab - fisicaechimica.unipa.it · raman spectroscopy lab ... manual xyz...
TECNOLOGIA RAMAN TECNOLOGIA RAMAN
Simonpietro Agnello
Raman Spectroscopy lab
28/09/2016
Department of Physics and Chemistry – University of Palermo � https://fisicaechimica.unipa.it/agnello/
Master degree Physics 1996 - Palermo
PhD Physics 2001 – Palermo
2001-2003 Post-doc
2004-2014 University Researcher
2015 � Associate Professor
Who am I ?
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Solid state physics
Irradiation effects
Thermal effects
Optical spectroscopy (OA, PL, Raman)
Electron Paramagnetic Resonance
Who am I ?
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SiO2 (bulk, optical fibers, nanostructures,...)
Carbon based-materials
Materials for Photovoltaics
Who am I ?
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� http://www.unipa.it/lamp/
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When light interacts
With matter
Exchange of energy
could occur
What is Raman Spectroscopy ?
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The easiest case
What is Raman Spectroscopy ?
Elastic scattering
NO ENERGY EXCHANGE
What is Raman Spectroscopy ?
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The energy exchange
OCCURS
hν1
hν2
hν1 ≠ hν2
hν1 - hν2=energy of vibration/rotation
RAMAN EFFECT
What is Raman Spectroscopy ?
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Using single energy
photons (Laser light)
The energy exchange is
“easily” measured
What is Raman Spectroscopy ?
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What is Raman Spectroscopy ?
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What is Raman Spectroscopy ?
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Raman spectroscopy finds multiple applications thanks to the availability of LASER lines
It is a not invasive technique: Safe for sample, repetition of measurements
No sample preparation: gas, solid, liquid
Minimal samples quantitiy, also powder
NOT APPLIABLE TO METALS
Why Raman Spectroscopy ?
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http://www.horiba.com/it/scientific/products/raman-spectroscopy/applications/ �
Why Raman Spectroscopy ?
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SiO2
Crystal (quartz)
250 500 750 1000 1250 1500
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
Inte
nsità
(un
ità a
rbitr
arie
)
Energia vibrazione 1/λ (cm-1)
QUARZO
Amorphous (glass)
250 500 750 1000 1250 1500
0.0
0.2
0.4
0.6
0.8
Inte
nsità
(un
ità a
rbitr
arie
)
Energia vibrazione 1/λ (cm-1)
VETRO
Why Raman Spectroscopy ?
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0 500 1000 1500 2000 2500 3000 35000.0
0.5
1.0
OEVO00063G
FT
Ram
an S
catte
ring
Wavenumber (cm-1)
δ(=C-H) in plane C-H deformation
1269
Evib (cm-1)
1303
CH2 In phase twisting
1441
δ(CH2) scissoring
1657
ν(C=C) stretching
1748
ν(C=O) Stretching in ester
2852
ν(C-H) Stretching simm in CH2
2898
ν(C-H) Stretching simm in CH3
2929
ν(C-H) Stretching asimm in CH2
3009
ν(=C-H) Stretching simm in RCH=CHR
1269 1303 1441 1657 1748 2852 2898 2929 3009
FAT ACIDS LINOLEIC ADULTERATION
ossidazione
Natural oils
1500 1600 1700 1800 1900 20000.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
Inte
nsità
(un
ità a
rbitr
arie
)
Energia Vibrazione 1/λ (cm-1)
EXTRA VERGINE OLIVA SOIA GIRASOLE PALMA
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He-Cd
325nm
He-Ne
633nm
NdYAG
dupl.
532nm
Diode
785nm
Nd:YAG
1064nm (FTRaman)
Raman Spectroscopy LAB
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Raman Spectroscopy LAB
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Raman Spectroscopy LAB
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Raman Spectroscopy LAB
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Raman Spectroscopy LAB
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LABORATORY TOPICS ACTIONS OFFERED SERVICES APPLICATIONS
Raman
Spectroscopy
Laboratory
Principal:
LIFESCIENCE
PHARMACEUTICAL
FOOD
MATERIALS
SEMICONDUCTOR
FORENSIC
ENVIRONMENT
GEMOLOGY
MINERALOGY
CULTURALE HERITAGE
Not invasive Physical
characterization of any material
in gas, liquid or solid phase.
Synergetic activity with the
preparation and characterization
labs of MEDCHHAB.
Study of material properties and
changes for companies or within
research projects.
Development of novel materials
through their physical
characterization.
Analysis of materials at the
micrometric resolution both in
small and large samples.
- Material identification
- Material characterization
- Material analysis
- Structural analysis
- Spectroscopic 2D, 3D imaging
- In-situ analysis during thermal
treatment (-196÷600°C) in
controlled atmosphere
- Autonomous
- With staff
- Assisted
Material science,
characterization and quality
controls of the structural
characteristics.
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LASER
LINES
GRATINGS MICROSCOPES CELLS SOFTWARE
(semi-automatic
switch 10’)
1064 nm (500mW)
785 nm (100mW)
633 nm (17mW)
532 nm (100 mW)
325 nm (200 mW)
Dynamic range
0.01%-100%
950 l/mm (NIR)
600 l/mm (NIR, VIS, UV)
1800 l/mm (NIR, VIS, UV)
2400 l/mm (UV)
Stokes-Antistokes shift down to ~10 cm-1
for 785 nm, 633 nm, 532 nm
CONFOCAL
UPRIGHT Olympus
BXFM-ILHS:
MOTORIZED XYZ
LARGE SAMPLES
INVERTED Olympus IX71:
MANUAL XYZ
TEMPERATURE/PRESSURE
LINKAM:
(-196÷600°C) up to 14 BAR.
90° LIQUID CELL HOLDER
LABSPEC 6
BIORAD LIBRARIES
DETECTORS
OBJECTIVES
Back-illuminated-deep-depletion CCDs:
- Synapse (UV-VIS)
- Symphony II (NIR) N2 cooled
VIS-UV
10x (N.A. 0.25 W.D. 10.6)
50x (N.A. 0.75 W.D. 0.38)
100x (N.A. 0.90 W.D. 0.21)
VIS-NIR
20x (N.A. 0.5 W.D. 2.1)
40x (N.A. 0.75 W.D. 0.51)
UV
40x (N.A. 0.5 W.D. 1)
NIR
50x (N.A. 0.55 W.D. 8)
LWD VIS
20x (N.A. 0.40 W.D. 26.5)
50x (N.A. 0.50 W.D. 26.5)
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That’s all
Grazie
That’s all
Grazie
http://www.chab.center/home/
