QUIZ 1 Cell Structure

MCQ’s – X type (true or false)

1. The most common staining combination used on light microscope sections is haematoxylin and eosin

a) Regarding Haematoxylin :

1. It is a basic dye 2. It stains cell components that carry a positive charge 3. It stains cell components blue/purple colour 4. Its staining reaction is said to be basophilic 5. it stains RER

b) Regarding Eosin:

1. It is a basic dye 2. It stains cell components that carry a positive charge 3. It stains cell components a pink colour 4. Its staining reaction is said to be acidophilic 5. It stains chromatin

2. The main function of a cell with abundant rER and a well-developed Golgi apparatus would include:

1. Steroid hormone production 2. Protein production 3. Export of steroid hormones (out of the cell) 4. Export of proteins (out of the cell) 5. Post translational modification of proteins

3. The Plasma membrane

1. Consists of a single layer of lipids 2. Contains integral proteins 3. Carries a negative charge on its cytoplasmic surface 4. Its total thickness is 4-6nm 5. The outer surface has a glycocalyx

4. Regarding the Plasma membrane :

1. The lipid bilayer is amphatic 2. It contains peripheral proteins 3. Glycoproteins are found on the inner leaflet 4. It has lipid rafts 5. It contains ion channels

Short answer questions

1. Explain why you would use electron microscopy to visualize cells and their components rather than light microscopy. (2 marks)

2. Explain the difference between magnification and resolution (3 marks)

3. Draw a typical generic cell that is involved in protein production (e.g. antibodies) where the protein is modified then

exported from the cell. (Note: the key here is to relate structure to function). (4marks)

Spotters

1. Identify the large organelle labeled A (1 mark) 2. Identify the organelles labeled B and give their main function (2 marks) 3. Suggest an overall function for this cell (1 mark)

1. How would you describe the staining reaction of the large “fried egg “ cells (1 mark) 2. Is the cytoplasm of these cells largely acidic or basic? (1 mark) 3. How would you describe the nuclear staining reaction (1 mark) 4. How would you describe the staining reaction of the cytoplasm of the other cells present? (1 mark)

1. Identify the structure labeled mv in the upper electron micrograph and give a main function for this structure (2 marks)

2. The structure” J “is the same as the structure seen in the lower left micrograph; identify this stricture (1 mark)

3. What techniques are used to produce these structures? (1 mark)