PVP Hydrogels containing Nanosilver for the treatment of venous ulcers
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WOUND DRESSING Suitable Mechanical properties No cell adherence Antibacterial properties (nanosilver) Hydrogels comprise crosslinked polymeric matrices of renowned applications in distinct biotechnological areas, which may be synthesized by several techniques. In general and although very effective, many techniques required the need of monomers or solvents which require careful washing processes in order to be removed from the systems. On this aspect the synthesis of hydrogels developed by exposition to ionizing radiation offers advantages over the conventional methods, such as no need of monomers or toxic solvents, being also capable of sterilizing the product inside final packaging. In this work we report the loading and release of silver nanoparticles into radiation synthesized hydrogels to be applied as dressing for wound healing, from development stages to initial clinical evaluation in (Ethics Committee Reg TO 161/2013) patients with venous ulcers. PVP Hydrogels containing Nanosilver for the treatment of venous ulcers Research Approach Swelling Properties & Nanosilver Release References PVP Hydrogel & Nanosilver Gustavo H. C. Varca 1 , Maria J.A. de Oliveira 1 , Jorge G.S. Batista 1 , , Vinicius S. Carvalho 1 , Gabriela A. Dos Santos 1 , Talita R. Cardoso 2 , Albeliggia B. Vicentine 2 ,Ademar B. Lugão 1 [email protected] 1 Instituto de Pesquisas Energéticas e Nucleares – IPEN-CNEN/SP, SP, Brazil; 2 Instituto Tocantinense Presidente Antônio Carlos – ITPAC Porto Nacional, TO, Brazil. Figure 2. Hydrogel handling & usage. Figure 3. Mechanical Properties of the hydrogels. Figure 1. PVP based Hydrogels containing nanosilver obtained by simultaneous crosslinking and sterilization induced by gamma radiation. Conclusio ns The membrane produced presented suitable mechanical properties to be applied as wound dressings. The Patient ulcer treated with the dressings presented increased healing process and better aspects which were identified on the 5 th day. On the 10th day, the lesions were significantly smaller, with reduced odor and no report of pain. Thus the initial trials indicated that such dressing may be effectively applied for the treatment of venous ulcers, being capable of significantly reducing pain, odor and exudate formation. Clinical trial – The case of Venous Ulc Figure 7. Patient Venous ulcer during treatment (5 th day). Figure 8. Venous ulcer during treatment (10 th day). Figure 6. Patient Venous ulcer on the 1 st day of treatment with the dressing. Mechanical Properties Figure 5. Nanosilver Release Profile -ICP-MS. Figure 4. Swelling properties of the hydrogel - (a) lyophilized form (b) regular form. Hydrogel Synthesis Acknowledgments PVP hydrogel PVP hydrogel containing nanosilver *Commercially available nanosilver solution (22 ppm) from KHEMIA® imultaneous Polymer crosslinking & sterilization Gamma Irradiation dose (25 kGy) - 8% PVP - 1% PEG - 1.5% Agar - 22ppm Nanosilver* Hydrogel Formulation Polymer crosslinking & Sterilization Exudate absorption Reduced odor Dressings removal (every 24h No pain No bleeding Reduced venous ulcer size Increase in revitalized tiss 10 th day 5 th day Significantly reduced odor; Reduced exudate formation; Dressings removal (every 12h Reduced Sensitivity and pain 1st day Patient with 8.706 cm 2 venous ulcer (7 years) Dead tissue; Dressings removal (every 6h) Intense pain and odor bleeding Nanosilver release up to 48 hours Oliveira, M.J.A.; Amato, V.S. ; Lugão, A.B. ; Parra, D.F. Radiat. Phys. chem. v. 81, p. 1471- 1474, 2012. Ferraz, C.C; Varca, G.H.C.; Lopes, P.S.; Mathor, M.B.; Lugao. Radiat. Phys. chem. v. 94, p. 186- Gel Fraction Band gel PVP ± 69 % PVP / Ag + ± 75% 0 10 20 30 40 50 0 50 100 150 S w e lIin g (% ) Tim e (h ) P V P band gelP h = 6 P V P band gelP h = 7 P V P A g P h = 6 P V P A g P h = 7 B 0 10 20 30 40 50 0 50 100 150 S w elling(% ) Tim e (h ) P V P band gelP h = 6 P V P ban gelP h = 7 P V P A g P h = 6 P V P A g P h = 7 A 0 20 40 60 80 100 0 1 2 3 4 S tre n g h t (N ) T en sio n (% ) H yd ro ge ln a n o p a rticle s o f A g B and gel
description
Gustavo H. C. Varca 1 , Maria J.A. de Oliveira 1 , Jorge G.S. Batista 1 , , Vinicius S. Carvalho 1 , Gabriela A. Dos Santos 1 , Talita R. Cardoso 2 , Albeliggia B. Vicentine 2 ,Ademar B. Lugão 1. [email protected] 1 Instituto de Pesquisas Energéticas e Nucleares – IPEN-CNEN/SP, SP, Brazil; - PowerPoint PPT Presentation
Transcript of PVP Hydrogels containing Nanosilver for the treatment of venous ulcers
WOUND DRESSING
Suitable Mechanical properties
No cell adherence
Antibacterial properties (nanosilver)
0 10 20 30 40 500
50
100
150
Swel
Iing
(%)
Time (h)
PVP band gel Ph = 6 PVP band gel Ph = 7 PVP Ag Ph = 6 PVP Ag Ph = 7
B
Hydrogels comprise crosslinked polymeric matrices of renowned applications in distinct biotechnological areas, which may be synthesized by several techniques. In general and although very effective, many techniques required the need of monomers or solvents which require careful washing processes in order to be removed from the systems. On this aspect the synthesis of hydrogels developed by exposition to ionizing radiation offers advantages over the conventional methods, such as no need of monomers or toxic solvents, being also capable of sterilizing the product inside final packaging. In this work we report the loading and release of silver nanoparticles into radiation synthesized hydrogels to be applied as dressing for wound healing, from development stages to initial clinical evaluation in (Ethics Committee Reg TO 161/2013) patients with venous ulcers.
PVP Hydrogels containing Nanosilver for the treatment of venous ulcers
Research Approach
Swelling Properties & Nanosilver Release
References
PVP Hydrogel & Nanosilver
Gustavo H. C. Varca1, Maria J.A. de Oliveira1, Jorge G.S. Batista1, , Vinicius S. Carvalho1, Gabriela A. Dos Santos1, Talita R. Cardoso2, Albeliggia B. Vicentine2,Ademar B. Lugão1
[email protected] de Pesquisas Energéticas e Nucleares – IPEN-CNEN/SP, SP, Brazil;
2Instituto Tocantinense Presidente Antônio Carlos – ITPAC Porto Nacional, TO, Brazil.
Figure 2. Hydrogel handling & usage.
Figure 3. Mechanical Properties of the hydrogels.
Figure 1. PVP based Hydrogels containing nanosilver obtained by simultaneous crosslinking and sterilization induced by gamma radiation.
ConclusionsThe membrane produced presented suitable mechanical
properties to be applied as wound dressings. The Patient ulcer treated with the dressings presented increased healing process and better aspects which were identified on the 5th day. On the 10th day, the lesions were significantly smaller, with reduced odor and no report of pain. Thus the initial trials indicated that such dressing may be effectively applied for the treatment of venous ulcers, being capable of significantly reducing pain, odor and exudate formation.
Clinical trial – The case of Venous Ulcers
Figure 7. Patient Venous ulcer during treatment (5th day).
Figure 8. Venous ulcer during treatment (10th day).
Figure 6. Patient Venous ulcer on the 1st day of treatment with the dressing.
Mechanical Properties
Figu
re 5
. Nan
osilv
er
Rele
ase
Profi
le -I
CP-M
S.
Figure 4. Swelling properties of the hydrogel - (a) lyophilized form (b) regular form.
Hydrogel Synthesis
Acknowledgments
0 10 20 30 40 500
50
100
150
Swel
ling(
%)
Time (h)
PVP band gel Ph = 6 PVP ban gel Ph = 7 PVP Ag Ph = 6 PVP Ag Ph = 7
A
0 20 40 60 80 1000
1
2
3
4
Stre
nght
(N)
Tension (%)
Hydrogel nanoparticles of Ag Band gelPVP hydrogel
PVP hydrogel containing nanosilver
*Commercially available nanosilver solution (22 ppm) from KHEMIA®
Simultaneous Polymer crosslinking & sterilization Gamma Irradiation dose (25 kGy)
- 8% PVP- 1% PEG- 1.5% Agar- 22ppm Nanosilver*
Hydrogel Formulation
Polymer crosslinking & Sterilization
Exudate absorption
Reduced odor Dressings removal (every 24h) No pain No bleeding Reduced venous ulcer size Increase in revitalized tissue
10th day
5th day Significantly reduced odor; Reduced exudate formation; Dressings removal (every 12h) Reduced Sensitivity and pain
1st day Patient with 8.706 cm2 venous
ulcer (7 years) Dead tissue; Dressings removal (every 6h) Intense pain and odor bleeding
Nanosilver release up to 48 hours
Oliveira, M.J.A.; Amato, V.S. ; Lugão, A.B. ; Parra, D.F. Radiat. Phys. chem. v. 81, p. 1471-1474, 2012.Ferraz, C.C; Varca, G.H.C.; Lopes, P.S.; Mathor, M.B.; Lugao. Radiat. Phys. chem. v. 94, p. 186-189, 2014.
Gel Fraction
Band gel PVP ± 69 % PVP / Ag+ ± 75%
