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LAMPIRAN

 

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LAMPIRAN 1 ROADMAP PENELITIAN

 

Sumber kolagenase organisme perairan

Purifikasi dan karakterisasi Kolagenase

Kelenjar pencernaan Simpson (2000):

Kepiting, lobster, udang

Hepatopankreas Aoki (2003) :

Udang (Pandalus eolus) Rudenskaya (2004) :

Kepiting raja (Paralithodes camtschaticus)

Organ dalam Kim (2002):

filefish (Novodon modestrus)

Park (2002): makerel

(Scomber japanicus)

Pilorik kaeka: Byun (2002)

Tuna (Thunnus thynnus)

KOLAGENASE

Usus Pilorik kaeka Hati

Bandeng (Chanos chanos, forskal)

Jaringan Kim (1991):

(Novodon modestrus)

Yuniarti (2008)

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Lampiran 2 Tabel Konversi dari g (gravity) ke rpm (revolutions per minute) Konversi dari g ke rpm berdasarkan rumus:

Dimana: g = gravity atau gaya sentrifugal relatif (RCF = Relative Centrifugal

Force) R = jari-jari rotor (cm) S = kecepatan putaran dalam rpm (revolutions per minute)

  

Sumber: Pierce Biotechnology, Inc. 2005. Technical Resource Convert Between

Times Gravity (×G) And Centrifuge Rotor Speed (Rpm). Rockford, USA: Pierce Biotechnology, Inc. www.piercenet.com.

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Lampiran 3

57

Lampiran 4 Organ dalam ikan bandeng (Chanos chanos, Forskal)

 

 

Hati Pilorik kaeka

Usus

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Lampiran 5 Hasil perhitungan elekroforesis  

No Marker Batas bawah

(cm) Batas atas

(cm) BM Rf Log BM 1 Phosphorilase b 5 0,7 97.000 0,14 4,9867722 Albumin 5 1,5 66.000 0,3 4,8195443 Oval bumin 5 2,3 45.000 0,46 4,653213

4 Carbonic anhidrase 5 3,1 30.000 0,62 4,477121

5 tripsin inhibitor 5 3,4 20.100 0,68 4,3031966 α-laktalbumin 5 4,0 14.400 0,80 4,1583627 Band 1 5 3,0 27.612,14 0,60 4,44110 8 Band 2 5 4,1 14.636,59 0,82 4,16544

   

Kurva regresi linear berat molekul marker dan Rf

y = -1,253x + 5,1929R2 = 0,9828

0

1

2

3

4

5

6

0 0,2 0,4 0,6 0,8 1

Rf

Log BM