Honglei Huang, Mukram M Mackeen, Matthew Cook, Eniyou Oriero, Emily Locke, Marie L Thézénas, Benedikt M Kessler, Davis Nwakanma2 and Climent Casals-Pascual

Emmanuel Alvarez Londoño Christian Camilo Cano Granda

Malaria 655000 deaths yearly Standard diagnostic method Visualization

of the parasite in blood Study Plasmodium falciparum DNA in

saliva from malaria patients Saliva could correctly identify up to 82% of

micoscopy positive samples Salivary proteome identify Sjrogren’s

syndrome

Plasmodium Genus of apicomplexan parasites. This parasite cause malaria infection. Of the over 200 known species, 11 infect humans

Falciparum Parasit protozoo and one of the plasmoduim species that causes malaria. Is transmited by anopheles mosquito

Proteomic Large scale study of proteins, particularly their structures and functions

Biomarker Substance used how biological state indicator Malaria Disease caused by parasites of Plasmodium

genus In this study, the researchers used proteomic to find

important biomarkers of malaria infection by P.falciparum

Malaria is a disease that kills many people every year, especially in Africa, In this article we study a noninvasive diagnostic method and much faster, which is to find biomarkers in saliva of malaria host,with depletion of other abundant proteins in saliva

• Estudio de la población• Muestras de sangre• Individuos• Microscopia• Agotamiento de amilasa

• Agotamiento de lectinas• Precipitación de proteínas• Electroforesis en SDS- PAGE

LECTINAS:

• Concavalina (Con A): Glu

• Jacalina (Jac): O-glicanos, Igs, mucina

• Aglutinina de cacahuete (ANP): Gal-Beta

arachis hipogaea

• Aleuria Aurantia (AAL): L-fucosa, hongo

• Agotamiento de amilasa y lectinas• AAL y JAC • Con A y ANP

• PFL0480w • PF08_0054• PFI0875w

Author What he said Yes / not

Deutsch O, et al

Krief G, et al

The presence of highly abundant

proteins in saliva, alpha-amylase in particular, is a clear

obstacle to identify potential protein biomarkers that

may be less abundant

NOT

von Itzstein, et al

Godwa DC

Macedo CS, et al

Although the extent of glycosylation of Plasmodium spp

proteins is largely unresolved, there is evidence

suggesting the absence of glycosylation (N- and O-

linked) in P. falciparum.

Analysis of the Plasmodium genome demonstrates a lack

of the necessary glycosyl transferases required to

catalyse this reaction.

NOT

Kief G, et al Other proteins such as albumin and Ig may have masking

effects on the identification of less abundant proteins

NOT

Fu Q, et al Adding a prior step to deplete albumin from saliva by

sodium chloride/ethanol albumin depletion may prove a

useful strategy to increase the identification of parasite

proteins

YES

Noninvasive diagnostic methods are effective to see important biomarkers in different patologies

Depletion facilitates proteomic study of parasite proteins

It will be a quickly diagnostic tool and prevent disease progression

Here we can see the future of the noninvasive methodes in clinical diagnostics

GR Guile, Harvey DJ, O'Donnell, N, Powell AK, Hunter AP, Zamze S, Fernandes DL, Dwek RA, ala DR. Identificación de los altamente fucosilados N-oligosacáridos de la glándula parótida humana. Eur J Biochem. de 1998. En: The Public-Access Computer Systems Review [en linea]. Miami, FL. <http://www.ncbi.nlm.nih.gov/pubmed/9874230> [consulta: 14 Agosto 2012]

Alonzi DS, Neville DC, Lachmann HR, Dwek RA, Butters TD. Glucosilada oligosacáridos libres son los biomarcadores de retículo endoplásmico, la inhibición de la alfa-glucosidasa. Biochem J. 2008. En: The Public-Access Computer Systems Review [en linea]. Miami, FL. <http://www.ncbi.nlm.nih.gov/pubmed/17868040> [consulta: 14 Agosto 2012]