Protein Structure IST 444. Protein Chemistry Basics Proteins are polymers consisting of amino acids...
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Transcript of Protein Structure IST 444. Protein Chemistry Basics Proteins are polymers consisting of amino acids...
Protein Structure
IST 444
Protein Chemistry Basics
• Proteins are polymers consisting of amino acids linked by peptide bonds
• Each amino acid consists of:– a central carbon atom– an amino group NH2
– a carboxyl group COOH– a side chain (R group)
• Differences in side chains distinguish different amino acids.
O H O H O H O H O H O H O H
H3N+ CH C N CH C N CH C N CH C N CH C N CH C N CH C N CH COO-
Asp Arg Val Tyr Ile His Pro Phe D R V Y I H P F
Protein sequence: DRVYIHPF
repeating backbone structure
repeating backbone structure
CH2 CH2 CH CH2 H C CH3 CH2 CH2 CH2 CH2
COO- CH2 H3C CH3 CH2 HC CH CH2
CH2 CH3 HN N OH NH CH
C
NH2 N+H2
Hydrophobic stays inside, while hydrophilic stay close to water
Oppositely charged amino acids can form salt bridge.
Polar amino acids can participate hydrogen bonding
Side Chains Determine Structure
Steps in Obtaining Protein Structure
Target selection
Obtain, characterize protein
Determine, refine, model the structure
Deposit in repository
Domain, Fold, Motif
• A protein chain could have several domains– A domain is a discrete portion of a protein, can fold
independently, possess its own function
• The overall shape of a domain is called a fold. There are only a few thousand possible folds.
• Sequence motif: highly conserved protein subsequence
• Structure motif: highly conserved substructure
Protein Data BankProtein structures, solved using experimental techniques
Unique structural folds
Different structural folds
Same structural folds
Protein Structure Determination
• High-resolution structure determination– X-ray crystallography (~1Å)– Nuclear magnetic resonance (NMR) (~1-2.5Å)
• Low-resolution structure determination– Cryo-EM (electron-microscropy) ~10-15Å
X-ray crystallography• most accurate
• An extremely pure protein sample is needed.
• The protein sample must form crystals that are relatively large without flaws. Generally the biggest problem.
• Many proteins aren’t amenable to crystallization at all (i.e., proteins that do their work inside of a cell membrane).
• ~$100K per structure
Nuclear Magnetic Resonance
• Fairly accurate
• No need for crystals
• limited to small, soluble proteins only.
Protein Structure Visualization
• http://www.umass.edu/microbio/chime/top5.htm
• http://molvis.sdsc.edu/visres/• Rasmol• Chime• Protein Explorer• DeepView• JmolJava
Secondary Structure Prediction
• Rules developed from PDB data• Chou and Fasman (1974) developed an
algorithm based on the frequencies of amino acids found in a helices, b-sheets, and turns.
• Proline: occurs at turns, but not in a helices.• http://prowl.rockefeller.edu/aainfo/chou.htm• Modern algorithms: use multiple sequence
alignments and achieve higher success rate (about 70-75%)
Ramachandran Plot
a way to visualize dihedral angles φ (phi) against ψ (psi) of amino acid residues in protein structure.
Chou Fasman 1974• measured frequencies at which each amino acid appeared in
particular types of secondary sequences in a set of proteins of known structure
• assigns the amino acids three conformational parameters based on the frequency at which they were observed in alpha helices, beta sheets and beta turns – P(a) = propensity to form alpha helices – P(b) = propensity to form beta sheets – P(turn) = propensity to form beta turns
• also assigns 4 turn parameters based on frequency at which they were observed in the first, second, third or fourth position of a beta turn – f(i) = probability of being in position 1 – f(i+1) = probability of being in position 2 – f(i+2) = probability of being in position 3 – f(i+3) = probability of being in position 4
.
A.A.P(a) P(b) P(turn) f(i) f(i+1) f(i+2) f(i+3)
Alanine 142 83 66 0.060 0.076 0.035 0.058
Arginine 98 93 95 0.070 0.106 0.099 0.085
Asparagine 67 89 156 0.161 0.083 0.191 0.091
Aspartic acid 101 54 146 0.147 0.110 0.179 0.081
Cysteine 70 119 119 0.149 0.050 0.117 0.128
Glutamic acid 151 37 74 0.056 0.060 0.077 0.064
Glutamine 111 110 98 0.074 0.098 0.037 0.098
Glycine 57 75 156 0.102 0.085 0.190 0.152
Histidine 100 87 95 0.140 0.047 0.093 0.054
Isoleucine 108 160 47 0.043 0.034 0.013 0.056
Leucine 121 130 59 0.061 0.025 0.036 0.070
Lysine 114 74 101 0.055 0.115 0.072 0.095
Methionine 145 105 60 0.068 0.082 0.014 0.055
Phenylalanine 113 138 60 0.059 0.041 0.065 0.065
Proline 57 55 152 0.102 0.301 0.034 0.068
Serine 77 75 143 0.120 0.139 0.125 0.106
Threonine 83 119 96 0.086 0.108 0.065 0.079
Tryptophan 108 137 96 0.077 0.013 0.064 0.167
Tyrosine 69 147 114 0.082 0.065 0.114 0.125
Valine 106 170 50 0.062 0.048 0.028 0.053
Chou Fasman isn’t Perfect
• Accuracy = 50-85%, depending on the protein
• http://npsa-pbil.ibcp.fr/NPSA/npsa_references.html
• Software and sites for protein predictions
GOR (Garnier, Osguthorpe and Robson)
• Another commonly used algorithm, uses a window of 17 amino acids to predict secondary structure
• rationale: experiments show each amino acid has a significant effect on the conformation of amino acids up to 8 positions in front or behind it.
• a collection of 25 proteins of known structure was analyzed, and the frequency at which each amino acid was found in helix, sheet, turn or coil within the 17 position window was determined – this creates a 17 *20 scoring matrix that is used to
calculate the most likely conformation of each amino acid within the 17 a.a. window
• This window slides down the primary sequence, scoring the most likely conformation for each amino acid based on the neighboring amino acids.
• Accuracy is about 65%
Signal for a Coiled Region
• Gapped in multiple alignments• Small polar residues
–Ala
–Gly (v. small so flexible)
–Ser
–Thr • Prolines rarer in other kinds of secondary
structure
How to Find Patterns Mathematically
Hidden Markov Models
• Hidden Markov Models (HMMs) are a more sophisticated form of profile analysis.
• Rather than build a table of amino acid frequencies at each position, they model the transition from one amino acid to the next.
• Pfam is built with HMMs.
Hidden Markov Models
Sample ProDom Output
Discovery of new Motifs
• All of the tools discussed so far rely on a database of existing domains/motifs
• How to discover new motifs– Start with a set of related proteins– Make a multiple alignment– Build a pattern or profile
Depicting Structure
Beta Sheet
Helix
LoopPDB ID: 12as
PDB New Fold Growth
• Only a few thousand unique folds in nature
• 90% of new structures deposited to PDB in the past three years have similar structural folds
New fold
Old fold
• Secondary structure is context-dependent
• Elements may be predicted to ID topology
• Generally only 50% of a structure is alpha-helix or beta-sheet.
• Beta-strands have necessarily longer range associations.
Secondary Structure• Protein secondary structure takes one of
three forms: Alpha helix Beta pleated sheet Turn
• 2ndary structure is predicted within a small window
• Many different algorithms, not highly accurate• Better predictions from a multiple alignment
Signals for Alpha Helices
• Amphipathic helices interact with core and solvent– Characteristic
hydrophobicity profile
• Prolines disrupt the middles of helices
Signals for beta strands
• Edge strands alternate hydrophobic/hydrophilic
• Center strands all hydrophobic
• Strands are extended so few residues per core span
Antiparallel Beta Sheet Parallel Beta Sheet
Peptide chains have a directionality conferred by their N-terminus and C-terminus. β strands can be said to be directional, indicated by an arrow pointing toward the C-terminus.
Adjacent β strands can form hydrogen bonds in antiparallel, parallel, or mixed arrangements.
Antiparallel β strands alternate directions so that the N-terminus of one strand is adjacent to the C-terminus of the next. This produces the strongest inter-strand stability because it allows the inter-strand hydrogen bonds between carbonyls and amines to be planar, which is their preferred orientation.
Beta Sheet (Antiparallel)
R groups don’t form these secondary structures, but block formation of the secondary
structures . The bonds forming the structures are from the amino and carboxy groups of the amino acid residues.
Signal for a Beta Strand
Creating Beta Sheets
• Large aromatic residues (Tyr, Phe and Trp) and β-branched amino acids (Thr, Val, Ile) are favored to be found in β strands in the middle of β sheets. Interestingly, different types of residues (such as Pro) are likely to be found in the edge strands in β sheets
Protein Classification
• Family: homologous, same ancestor, high sequence identity, similar structures
• Super Family: distant homologous, same ancestor, sequence identity is around 25%-30%, similar structures.
• Fold: only shapes are similar, no homologous relationship, low sequence identity.
• Protein classification databases: Pfam, SCOP, CATH, FSSP
Pfam
• http://www.sanger.ac.uk/Software/Pfam/
• Protein sequence classification database
• As of Pfam 24.0 (October 2009, 11912 families)
• Multiple sequence alignment for each family, then modeled by a HMM model
SCOP: Structural Classification of Proteins
http://scop.mrc-lmb.cam.ac.uk/scop/Protein structure classification database, manually curated110800 Domains, 38221 PDB entries
Class # folds # superfamilies # families
All alpha proteins 284 507 871
All beta proteins 174 354 742
Alpha and beta proteins (a/b) 147 244 803
Alpha and beta proteins (a+b) 376 552 1055
Multi-domain proteins 66 66 89
Membrane and cell surface 58 110 123
Small proteins 90 129 219
Total 1195 1962 3902
SCOP
• Nearly all proteins have structural similarities with other proteins and, in some of these cases, share a common evolutionary origin.
• The SCOP database, created by manual inspection and automated methods, aims to provide a detailed and comprehensive description of the structural and evolutionary relationships between all proteins whose structure is known.
• SCOP provides a broad survey of all known protein folds, detailed information about the close relatives of any particular protein, and a framework for future research and classification.
The Problem• Protein functions determined
by 3D structures
• ~ 30,000 protein structures in PDB (Protein Data Bank)
• Experimental determination of protein structures time-consuming and expensive
• Many protein sequences available
sequence
proteinstructure
function
medicine
Protein Structure Prediction
• In theory, a protein structure can be solved computationally
• A protein folds into a 3D structure to minimizes its free potential energy
• The problem can be formulated as a search problem
for minimum energy– the search space is enormous– the number of local minima increases exponentially
Computationally it is an exceedingly difficult problem
Who Cares?• Long history: more than 30 years• Listed as a “grand challenge” problem• IBM’s big blue• Competitions: CASP (1992-2006)
• Useful for– Drug design– Function annotation– Rational protein engineering– Target selection
Observations• Sequences determine structures
• Proteins fold into minimum energy state.
• Structures are more conserved than sequences. Two protein with 30% identity likely share the same fold.
What determines structures?
• Hydrogen bonds: essential in stabilizing the basic secondary structures
• Hydrophobic effects: strongest determinants of protein structures
• Van der Waal Forces: stabilizing the hydrophobic cores
• Electrostatic forces: oppositely charged side chains form salt bridges
Protein Structure Prediction• Stage 1: Backbone
Prediction– Ab initio folding– Homology
modeling– Protein threading
• Stage 2: Loop Modeling
• Stage 3: Side-Chain Packing
• Stage 4: Structure Refinement
The picture is adapted from http://www.cs.ucdavis.edu/~koehl/ProModel/fillgap.html
State of The Art• Ab inito folding (simulation-based method)
1998 Duan and Kollman36 residues, 1000 ns, 256 processors, 2 monthsDo not find native structure
• Template-based (or knowledge-based) methods– Homology modeling: sequence-sequence alignment,
works if sequence identity > 25%
– Protein threading: sequence-structure alignment, can go beyond the 25% limit
Sample Structure Prediction
....,....1....,....2....,....3....,....4....,....5....,....6 AA |MMSGAPSATQPATAETQHIADQVRSQLEEKYNKKFPVFKAVSFKSQVVAGTNYFIKVHVG| PHD sec | HHHHHHHHHHHHHHHH EEEEEEEEEEEEE EEEEEEEE | Rel sec |999997899667599999999989997655877843368889999999233399999658| detail: prH sec |000000000221289999999989998762011111000000000000000000000000| prE sec |000000000000000000000000000010000023578889989888536699999720| prL sec |999898889777600000000010001126888865311110000000363300000278| subset: SUB sec |LLLLLLLLLLLLLHHHHHHHHHHHHHHHHLLLLL...EEEEEEEEEEE....EEEEEELL| ACCESSIBILITY 3st: P_3 acc |bbebbeeeeeebbeebbebbeebeeebeeeeeee eebebbebebbbbbb bbbbeb bb| 10st: PHD acc |007006778670077007007706760777777737707007060000005000060500| Rel acc |103021343252044604644672424555547615444425212186671016926120| subset: SUB acc |.......e..e..eeb.ebbeeb.e.beeeeeee.eebeb.e....bbbb...bb.b...|
“Super-secondary” Structure
• Common structural motifs– Membrane spanning (GCG= TransMem)
– Signal peptide (GCG= SPScan)
– Coiled coil (GCG= CoilScan)
– Helix-turn-helix (GCG = HTHScan)
Transmembrane Structures
Signal Peptide
Coiled Coil
Helix Turn Helix
Fig. 9.23
Finding Information in Protein Sequences
There Are Many Meaningful Protein Signals
• Predicting protein cleavage sites
• Predicting signal peptides
• Predicting transmembrane domains
Signal Peptides
• Proteins have intrinsic signals that govern their transport and localization in the cell.
• Noble Prize to Gunter Blobel in 1999 for describing protein signaling.
• Proteins have to be transported either out of the cell, or to the different compartments - the organelles - within the cell.
Signal Peptides
• Newly synthesized proteins have an intrinsic signal that is essential for governing them to and across the membrane of the endoplasmic reticulum, one of the cell’s organelles.
• How do large proteins traverse the tightly sealed, lipid-containing, membranes surrounding the organelles?
Signal Peptides
• The signal consists of a peptide: a sequence of amino acids in a particular order that form an integral part of the protein.
• Specific amino acid sequences (topogenic signals) determine whether a protein will pass through a membrane into a particular organelle, become integrated into the membrane, or be exported out of the cell.
Signal Peptides
• Software exists that can predict the signal peptide sequences.
• The SignalP World Wide Web server predicts the presence and location of signal peptide cleavage sites in amino acid sequences from different organisms:– Gram-positive prokaryotes– Gram-negative prokaryotes– Eukaryotes.
Signal Peptides
• The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction based on a combination of several artificial neural networks.
• Artificial neural networks are collections of mathematical models that emulate some of the observed properties of biological nervous systems and draw on the analogies of adaptive biological learning.
Patterns in Unaligned Sequences
• Sometimes sequences may share just a small common region– common signal peptide– new transcription factors
• MEME: San Diego Supercomputing Facility
– http://www.sdsc.edu/MEME/meme/website/meme.html
• MEME uses Hidden Markov Models
Protein Secondary Structure• CATH (Class, Architecture,Topology,
Homology) http://www.biochem.ucl.ac.uk/dbbrowser/cath/
• SCOP (structural classification of proteins) -hierarchical database of protein folds http://scop.mrc-lmb.cam.ac.uk/scop
• FSSP Fold classification using structure-structure alignment of proteins http://www2.ebi.ac.uk/fssp/fssp.html
• TOPS Cartoon representation of topology showing helices and strands
• http://tops.ebi.ac.uk/tops/
Protein Sequence Hierarchy
SUPERFAMILY
FAMILY
DOMAIN
FOLD or MOTIF
Active SITE
RESIDUE
Protein families
• Proteins can be divided into families by:– Sequence.– Structure.– Function.
• Secondary databases divide proteins into families.
Protein families
• Types of secondary databases:
• “Curated” databases: Expert judgment of each family (Prosite, prints, Pfam).
• “Automated” databases: Constructed automatically (Blocks, ProDom).
Prosite• Characterization of protein families by conserved
motifs observed in a multiple sequence alignments of known homologues.
• Each family is defined by a single pattern.
• Motifs:
Prosite
• Each entry includes: Pattern and sometimes also a profile.
• Pattern is a method for describing a conserved sequence (consensus, profile).
• Sample entry
Prosite Structure
• Entries are divided into two files
– Pattern file: the pattern and all Swiss-Prot matches.
– Documentation file: Details of the characterized family, a description of the biological role of the chosen motif, references.
Prosite
• Pattern are described using regular expressions.
• Example:W-x(9,11)-[FYV]-[FYW]-x(6,7)-[GSTNE]
• Regular expressions retain only conserved or significant residue information
Prosite
A A C T T G
A A G T C G
C A C T T C
1 2 3 4 5
A 0.66 1 0 0 .
T 0 0 0 1 .
C 0.33 0 0.66
0 .
G 0 0 0.33
0 .
A A C T T G
[AC-]A-]GC[-T-]TC[-]GC[
multiple alignment
consensus
pattern
profile
•Sensitivity:
consensus<pattern<profile
Prosite Syntax The standard IUPAC one-letter codes.
`x' : any amino acid.
`[]' : residues allowed at the position.
`{}' : residues forbidden at the position.
`()' : repetition of a pattern element are indicated in parenthesis. X(n) or X(n,m) to indicate the number or range of repetition.
`-' : separates each pattern element.
`‹' : indicated a N-terminal restriction of the pattern.
`›' : indicated a C-terminal restriction of the pattern.
`.' : the period ends the pattern.
Prosite Syntax - Examples
• [AC]-x-v-x(4)-{ED}.• [Ala or Cys]-any-val-any-any-any-any-any but
Glu or Asp
• <A-x-[ST](2)-x(0,1)-v • N-terminus-Ala-any-[Ser or Thr]-[Ser or Thr]-
(any or none)-val
Searching with Regular Expressions
• Ideally the pattern should only detect true positives.
• Creating a regular expression that performs well in database searches is a compromise between sensitivity and tolerance (false positives and false negatives).
• The fuzzier the pattern, the noisier its result, but the greater the chances of finding distant relatives
Prosite
Searching Prosite
Input: Protein sequence
Output: list of patterns
Input: A pattern
Output: list sequences
BLOCKS
• Blocks are multiply aligned un-gapped segments corresponding to the most highly conserved regions of proteins
Blocks
• Blocks of 5-200 aa long alignments.
• A family is characterized by a group of blocks.
BLOCKS Construction
• Creation of BLOCKS by automatically detecting the most highly conserved regions of each protein family
• Blocks incorporates all known families from the “curated” databases.
Blocks
Searching Blocks
Input: Protein sequence
Output: list of blocks
Input: A Block
Output: list sequences
InterPro
• Integrated resource of Protein Families
• Unifies a set of secondary databases using same terminology.
• InterPro provides text and sequence based searches.
Conclusions
• Secondary databases are useful for characterizing of protein sequences.
• Numerous databases describe protein families.
• “Curated” databases do not include all known families.
• Secondary databases are useful for testing new user-defined motifs.