Protective effect of KIR3DS1 in asymptomatic HIV-1 seroconverters towards AIDS
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POSTER PRESENTATION Open Access Protective effect of KIR3DS1 in asymptomatic HIV-1 seroconverters towards AIDS M Stalinraja 1 , N Kalyanaraman 1 , P Leishman 1 , M Suresh 2 , M Jayalakshmi 1* From First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012) Chennai, India. 20-22 January 2012 Background The extreme variability at the Killer cell Immunoglobu- lin like Receptor (KIR) expressed on the surface of nat- ural killer cells interacts with Human Leukocyte Antigen (HLA) Class I appear to play a crucial role in the outcome against viral infection. The combination of HLA/KIR gene products either individually or collec- tively have been implicated in the control of HIV-1 in various populations. Objective of this immunogenetic case-control study is to determine the association between KIR3DS1 and HIV-1 asymptomatism. Methods 31 treatment naive HIV-1 asymptomatic (>3yrs without disease progression) and 31 HIV-1 seronegative ethnic matched healthy controls were recruited for this case – control study. Genomic DNA was extracted from per- ipheral blood by salting out procedure and KIR genotyp- ing was performed for 16 KIR genes (Activating, Inhibitory and Pseudogenes) by Duplex PCR sequence- specific primer method. Results Overall, we observed 66.12 % of individuals with B KIR haplotype among case and controls group. Among the 16 KIR genes were typed KIR3DS1 (Activating KIR gene) shows significant variation among HIV asymptomatic individuals was 74.2 % whereas 54.8 % among controls. Conclusion Since, KIR3DS1 is well known for its effect on direct viral containment, presence of higher KIR3DS1 is one of the possible reasons for asymptomatism among this study cohort. Author details 1 Dept of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai - 21, India. 2 ART Centre, Govt Theni Medical College and Hospital, Theni, India. Published: 4 May 2012 doi:10.1186/1471-2334-12-S1-P69 Cite this article as: Stalinraja et al.: Protective effect of KIR3DS1 in asymptomatic HIV-1 seroconverters towards AIDS. BMC Infectious Diseases 2012 12(Suppl 1):P69. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit * Correspondence: [email protected] 1 Dept of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai - 21, India Full list of author information is available at the end of the article Stalinraja et al. BMC Infectious Diseases 2012, 12(Suppl 1):P69 http://www.biomedcentral.com/1471-2334/12/S1/P69 © 2012 Stalinraja et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Transcript of Protective effect of KIR3DS1 in asymptomatic HIV-1 seroconverters towards AIDS
POSTER PRESENTATION Open Access
Protective effect of KIR3DS1 in asymptomaticHIV-1 seroconverters towards AIDSM Stalinraja1, N Kalyanaraman1, P Leishman1, M Suresh2, M Jayalakshmi1*
From First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)Chennai, India. 20-22 January 2012
BackgroundThe extreme variability at the Killer cell Immunoglobu-lin like Receptor (KIR) expressed on the surface of nat-ural killer cells interacts with Human LeukocyteAntigen (HLA) Class I appear to play a crucial role inthe outcome against viral infection. The combination ofHLA/KIR gene products either individually or collec-tively have been implicated in the control of HIV-1 invarious populations. Objective of this immunogeneticcase-control study is to determine the associationbetween KIR3DS1 and HIV-1 asymptomatism.
Methods31 treatment naive HIV-1 asymptomatic (>3yrs withoutdisease progression) and 31 HIV-1 seronegative ethnicmatched healthy controls were recruited for this case –control study. Genomic DNA was extracted from per-ipheral blood by salting out procedure and KIR genotyp-ing was performed for 16 KIR genes (Activating,Inhibitory and Pseudogenes) by Duplex PCR sequence-specific primer method.
ResultsOverall, we observed 66.12 % of individuals with B KIRhaplotype among case and controls group. Among the 16KIR genes were typed KIR3DS1 (Activating KIR gene)shows significant variation among HIV asymptomaticindividuals was 74.2 % whereas 54.8 % among controls.
ConclusionSince, KIR3DS1 is well known for its effect on directviral containment, presence of higher KIR3DS1 is one of
the possible reasons for asymptomatism among thisstudy cohort.
Author details1Dept of Immunology, School of Biological Sciences, Madurai KamarajUniversity, Madurai - 21, India. 2ART Centre, Govt Theni Medical College andHospital, Theni, India.
Published: 4 May 2012
doi:10.1186/1471-2334-12-S1-P69Cite this article as: Stalinraja et al.: Protective effect of KIR3DS1 inasymptomatic HIV-1 seroconverters towards AIDS. BMC InfectiousDiseases 2012 12(Suppl 1):P69.
Submit your next manuscript to BioMed Centraland take full advantage of:
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at www.biomedcentral.com/submit
* Correspondence: [email protected] of Immunology, School of Biological Sciences, Madurai KamarajUniversity, Madurai - 21, IndiaFull list of author information is available at the end of the article
Stalinraja et al. BMC Infectious Diseases 2012, 12(Suppl 1):P69http://www.biomedcentral.com/1471-2334/12/S1/P69
© 2012 Stalinraja et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, andreproduction in any medium, provided the original work is properly cited.
