ProStar 325 Detector

Petro van Poppel – Field Support Specialist Friday, 21 April 2023

Overview

Introduction to PS 325 Detector

• Dual Wavelength Option• LC Verify Software• Flow cells• CommunicationsLC Verify

• Production Tests with 0x0 flow cell• Wavescan Test (Mono Options)• Diagnostics Mode (Main Tab)• Electronics Test (Input/Output)• System Details (EEPROM)• Communications (COMM)PS 335 Shipping Test

Problems

325 Optics Module Light Path

D2

Vis

325 Optics Ray Beam

Dual Wavelength Option

Dual wavelength is a firmware change that allows you to view 2 wavelengths “simultaneously”

Galaxie Software

LC Diagnostics Software

325/335 Back Panel

I/O adaptor board for the sync signals

P/N 02-101865-00

P/N 02-101875-00

Relay Adaptor board

Flow Cell Ray Beam

General design of the 9x0 flow cell General design of the 9x1 flow cell

Flow Cell Specs

Flow Cell Prep

(9 x 1)

Super Prep

(4 x 0.15)

Analytical

(9 x 0)

Micro

(4 x 0 )

Pressure rating

1000 psi 1000 psi 1000 psi 1000 psi

Flow Cell Volume

30 µl 145 µl 15 µl 1.5 µl

Material (non optical)

Ti & PEEK Ti & PEEK Ti & PEEK Ti & PEEK

Noise Performance

200 ml/min

10-500ml

1x10-4AU

800 ml/min

0.80-10 L

1x10-4AU

40 ml/min

0.1 µl-100 ml

5x10-3AU

1 ml/min

0-20 ml

7.5x10-3AU

Communications

TCP/IP communications for Detector:

•Static or Dynamic

•Networked or standalone

•Firewall

•Access on Network, OS or PC

LC Verify side

LC Diagnostics Side

Ctrl-Shift-F10 to enable Diagnostics sideCtrl-Shift-F9 to hide Diagnostics side

PS 325 Test Certificate

Test Certificate provided by factory

Parameters tested:1) Noise2) Wavelength Accuracy3) Wavelength Reproducibility4) Spectral Bandwidth

Accuracy5) AutoZero Precision Test6) Detector Linearity Test

LC Verify use in Final Testing

PS 325 Noise Test (FT)

PS 325 Wavelength Accuracy Test (FT)

PS 325 Wavelength Repeatability Test (FT)

PS 325 Spectral Bandwidth Test (FT)

PS 325 Auto Precision Test (FT)

PS 325 Detector Linearity Test (FT)

pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.

PS 325 LCVerify Wavescan

PS 325 Wavescan results

PS 325 Contributions from each Lamp

PS 325 LCVerify Diagnostics Mode

PS 325 LCVerify Input/Output Test

PS 325 Main PWB Power Supply LED’s

A: + 24 V power SupplyB: + 9V and – 9V Power SupplyC: + 5 V Power Supply

BA

C

Front of the Unit

PS 325 LCVerify System Details

PS 325 LCVerify Communications

Caffeine Shipping Test

PS 325 Caffeine Shipping Test:

Test made up of 1 components:

• Loop injection using 25 µg/ml caffeine

Test is conducted using Galaxie Software

PS 325 Lamp Intensity Test

Test 1: Lamp Intensities

PS 325/335 Caffeine Loop Injection Test

Test Calculates:Peak HeightPeak AreaNoiseS/N

Common Problems

•High Noise

•Lamp on, not ready

•SIG_CC_ZERO_ORDER_PEAK_FIND_FAILED

•SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL

•COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED

•9555 LMP_CC_D2_FILAMENT_FAILURE

•PROSTAR 325 DRIVER:  Collection not started - Acquisition time too short for the method.

•Foggy Optics

PS 325 Error Messages Table can be found:

• PS 325 Service Manual• Dealernet

Foggy Optics

What is Foggy Optics?

What is Foggy Optics?

Three types of Foggy optics

• White powdery coating

• Rust/brown spot burn

• Speckled mirror (delamination)

Foggy Optics

What is Foggy Optics?

Diagnosing Foggy Optics

Diagnosing Foggy Optics

Run a “wavescan” test,

When bad

Open the optics and inspect.

Wavescan test results

Intensity for the 254 nm lineCurrently is 10 – 50 K counts

False positive

New D2 vs Aged D2 Intensities

Foggy Optics

What is Foggy Optics?

Diagnosing Foggy Optics

Treating Foggy optics

0603 Project (Foggy Optics)

Proposed Fixes and their status:

White powder fogging: due to leaky flow cells and customer sample depositing on the mirrors

New Flow cells & fittings available for all cells except 4 x 0.15

Brown powder fogging: due to UV attack on some of the foam parts close to the UV source.

Eliminating as much of the foam as possible and shielding the rest.

Mirror delamination: due to UV attack on the thin Al coating of the mirrors

Eliminated by putting a NiCr coating on the mirrors to strengthen the Al coating.

Opening and cleaning the optics

Cleaning 325 optics

Clean area in circle

Do not adjust!!

Replacing Optics

Wavelength calibration data must be entered into the 335 EEPROM

Summary

•Single and Dual Wavelength, UV and UV/vis

•Optical path is open

•Flow cell is dual path

•Diagnostics Tests, like wavescan

•Shipping Test

•Main Problems (Foggy Optics)