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Project Stink-be-GoneJune training

Dr. Rebeccah Waterworth

Email address: rwater@umd.edu

Project website address:

http://shrewsburylab.weebly.com/

project-stink-be-gone.html

Street Address:

University of Maryland

4112 Plant Sciences Bldg

4291 Fieldhouse Dr.

College Park, MD 20742

Agenda1. Pre-quiz

2. Training with Powerpoint (Part 1)• Stink bug species

• Stink bug life cycle

• Egg mass “look-alikes”

• ID different stink bug egg masses

3. Walkabout outside to look for egg masses

4. Training with Powerpoint (Part 2)• Natural enemies of stink bug eggs

• Where to look for egg masses and on what hosts

• Field work lists

• Collecting periods

• The datasheet (site types, habitat features, wasp guarding)

• GPS coordinates

• Shipping via FedEx

5. Post-quiz

6. Evaluation of training

7. Pick-up kits for project

mailto:rwater@umd.eduhttp://shrewsburylab.weebly.com/project-stink-be-gone.html

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What are stink bugs?• Order Hemiptera

• Family Pentatomidae

• Herbivorous and predatory species

• Have a shield-shape to their body

• Named “stink” bugs because of the

odor released from glands on the

underside of their bodies

• Sometimes brightly colored

Anna Letaw Bill Johnson

Check out the stink bug look-alikes in your spiral notebook and at the training

Stink bug adultsp. 1p. 3p. 7

p. 12p. 19 p. 21

Page numbers refer to the

page in your stink bug book

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Stink Bug Life Cycle

Managing stink bugsBiological control (or biocontrol)

• Natural enemies include predators and parasitoids

• In Project Stink-be-Gone, we are interested in parasitoids

that attack the eggs of stink bugs

David R. Lance USDA APHIS PPQ David R. Lance USDA APHIS PPQElijah Talamas

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Collecting Egg Masses – Stink bugsBrown marmorated

stink bugs

(BMSB)

Harlequin stink

bugs (HQN)

Brown stink bugs

Spined soldier bug

(Podisus)

Look for the barrel shape when the eggs are viewed in profile

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Collecting Egg Masses –Stink bug egg “look-alikes”

But if you are in doubt as to whether an egg mass was laid

by a bug, beetle, or moth, please send it to me anyway!

Other bug eggsSee display at the end of the

training to see what these

bugs and their eggs look like!

• Various assassin bugs

• Including green assassin

bugs

• Leaf-footed bugs

• Squash bugs (no need to

collect and send these egg

masses)

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Other “stuff”

Braconid

wasp pupal

cases

Green

lacewing

pupal

cases

eulophid

wasp pupal

casesErineum galls

(sample is oak)

Some stink bug natural enemiesPredators Parasitoids

Chewing predator

= ground beetles

Piercing/sucking

Piercing/sucking

Other stink bugs

Spiders

See parasitoid

specimens

under the

microscope!

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Egg Masses Attacked by Natural Enemies

a: bugs hatched; b: feeding by a piercing sucking predators; c: chewing predation,

e: parasitiods emerged (note circular emergence holes and no egg burster)

Egg burster

Collecting Egg Masses – Where?

Edges are better places to search

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Collecting Egg Masses – What hosts?

Any mustards, including

sweet alyssum! Cleome, too

Pretty much everything!

Host Plants of BMSB

Trees/shrubs PerennialsAnnual

Flowering Plants

Annual,

VegetablesVines, Various

Cercis

canadensis (8)

Red raspberry

(2)Cleome (5) Tomato (1) Vitis riparia (2)

Acer rubrum (4)Wine

raspberry (2)Lantana (1)

Virginia

creeper (1)

Magnolia (1)Asclepias

syriaca (1)

Pigweed*

(Amaranthus) (1)

Tilia cordata (1)

Paulownia

tomentosa (2)

Cornus

racemosa (1)

Pyrus

calleryana (1)

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Collecting Egg Masses – What hosts?BMSB: polyphagous pest, but not known

to feed on conifers or ornamental grasses(partial list) Along wooded edges, check out tree

of heaven, sassafras, black cherry, black locust,

hackberry, catalpa, paulownia, Eastern redbud

Brown stink bug: polyphagous pest(partial list) sassafras, wineberry, Eastern

redbud, yellowwood, gray dogwood, red maple,

hackberry, sumac, butternut, boxelder, black

cherry, linden, paulownia, catalpa, raspberry,

black walnut, elm, peach

Green stink bug: polyphagous pest, BUT

not known to occur on conifers or oaks(partial list) Eastern redbud, black cherry,

red maple, sassafras, raspberry, persimmon,

Hydrangea paniculata, Washington hawthorn,

flowering dogwood, American witchhazel,

Carolina allspice, dogwood, apple, black walnut

Collecting Egg Masses – What hosts?

Rough stink bug: polyphagous pest(partial list) Eastern redbud, birch, red maple,

American sweetgum, tree of heaven, littleleaf

linden, paulownia, magnolia, dogwood,

Kentucky coffeetree,

Spined soldier bug: predator of other

insects but eggs laid on plants.(Partial list) Norway maple, littleleaf linden,

apple

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Field Work

What we supply What you should bring

Yellow cooler Clipboard

One ice pack Several datasheets

Petri dishes Pencils

Box to ship cooler Scissors

Pre-paid FedEx label Hand lens/loupe

Smart phone (for GPS coordinates)

Hand-held pruning shears or loppers

For safety and comfort:

Hat, long sleeves and pants

Sunscreen

Water

Insect/tick spray

Collecting Periods and Times• Search and collect egg masses any

time during the day

• Must be sure you can ship later that

day (or if you collect on Sundays –

first thing on Monday)

• Find one or two egg masses at a

time?

o Hold egg masses for up to one

week in the fridge

o Try to collect more eggs the

following week.

o Keep egg masses in petri dishes

inside cooler bag.

*Optional sampling period.*

Please consider searching

1 – 2 hours during this

period.

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Collecting Periods and Times

Prioritized sampling period = 6 weeks.

Please search one hour each week for

egg masses.

What are woods?

Yes!

Yes!

No!

No!

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The Datasheet• Each datasheet holds four tables or sections, one for

each of four different sites that you decide to visit.

• If you think that you might visit more than four

different sites when you sample for one hour, take

more copies of the datasheet with you.

Front Back

Site 1

Site 2

Site 3

Site 4

The Datasheet

When you have finished collecting egg masses and

filled in the datasheet, scan, copy, or take a picture of

your datasheet for your records.

Please send the original copy of the datasheet to me

IN YOUR COOLER.

If you visit a site and don’t see any egg masses, we

still would really like to know what site you visited:

approximate square area searched, a site description,

and features in that landscape. Please still fill out the

top two rows of that site section on your datasheet.

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Datasheet: Site TypesPrivate yardMultiple plant types – search equally

among herbaceous plants, shrubs, and

trees. If wooded edges are present,

search along those, too.

Park with woodsMultiple plant types – search equally

among any of the plant types present

Datasheet: Site TypesPark without woodsFairly simple landscape, but likely

multiple plant types are present –

search equally among any of the plant

types present

Wooded edge only• No ornamental plantings or

agriculture outside of the woods.

• Only search trees and shrubs that are

at the edge.

• Known hosts of BMSB are tree of

heaven, sassafras, black cherry,

hackberry, black locust, catalpa, and

paulownia

• No need to actually enter the woods.

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Datasheet: Site Types

Farm/Agriculture setting: • Corn and soybeans are some of the

dominant crops in Maryland.

• Search only along the edges of the fields, especially when corn

has developing ears and soybeans

have flowers and pods.

• Many farm fields have wooded

borders.

• If you can safely access those, please

search along those edges, too.

Feel free to collect in other

counties other than the one where

you are a Master Gardener. Just

write down the collection

information on your datasheet.

Search area measurements

160 feet

45 feet

130 feet

160+45+130 = 335 ft

335 ft x 2 ft = 670 ft2

75 feet

75 ft x 2 ft = 150 ft2

150 ft2 + 670 ft2

= 820 ft2

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How did I get those measurements?

Small ruler icon =

show ruler

Google Earth,

not Google

Maps

https://www.go

ogle.com/earth/

desktop/

How did I get those measurements?

Change ruler to

measure the

distance in feet.

https://www.google.com/earth/desktop/

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How did I get those measurements?

Read

measurement!

Habitat Features√ Paved surface

Manmade structure

? Flower trees

? Fruiting trees

√ Unmanaged trees

√ Managed trees

? Flower herb plants

? Flowering shrub

? Fruiting shrub

? Crop/vegetable

√ Managed turf

Open meadow

Stream/creek

Check all habitat features that were in

or bordering (within 5 feet) the area that

you searched

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Datasheet: Wasp Guarding?

• Try to catch the small

wasp (=parasitoid) if you

can and keep it with the

egg mass.

• If you can’t catch it,

that’s okay.

• But, please check the

box next to the egg mass

that you are collecting to

say that one was there.

Egg Mass is too Large for the Petri Dish!

Perfect fit!Some trimming

required!

Cutting is

preferred over

ripping the leaf

because the egg

mass is less

likely to pop off.

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Datasheet: GPS coordinates

Free Apps (for both iPhones and Android devices)

Google Maps

Waze

Here WeGo (Maps)

InRoute

Scout GPS

MapQuest

Please report by degrees North and degrees

West on your datasheet for each site.

Google Maps reports values in decimal form,

like this: 38.9889 (N) -76.9409 (W)

GPS Coordinates: Google Maps

Open Google Maps

so that it finds your

location

Place your finger and

hold on your location

(an address or ‘dropped

pin’ shows up)

Swipe up at the

bottom

North West

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Packing your Cooler• Shipping label is a giant sticker that is placed on the SIDE of your box.

• Your box will be sent to you with a label that is already filled out to send

samples to me.

• Remove and keep the top copy of the label (it says “Sender’s Copy” in

the top right corner.

• Feel free to use any

material to pad the

petri dishes and the

ice pack in your box.

• Balled up paper, paper

towels, and packing

peanuts are fine.

• If you hold the box

closed and shake it

(and you don’t hear

any rattling), the petri

dishes should ship

okay.

FedEx ShippingFedEx Express (orange) NOT FedEx Ground (green)

Zip code goes here

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FedEx Shipping

Green equals FedEx Ground.

Do not use any FedEx

Ground shipping times. Only use FedEx Express

(orange) shipping times.

FedEx Shipping

Mon Tues Wed Thurs Fri Sat Sun

Collect

and ship × × ×

Not

preferred

(for

shipping)

Not

preferred

(for

shipping)

Not

preferred

(for

shipping)

×(ship on

Mon.)

Arrives Wed Thurs Fri

Sat (but

no delivery

at Univ.)

Mon

(3 days

in transit-

too long)

Tues

(3 days in

transit –

too long)

Wed

FedEx Express 2day = 2 business days in transit