Progress in Common Bean Breeding for Angular Leaf Spot ... Progress in Common Bean Breeding for...

download Progress in Common Bean Breeding for Angular Leaf Spot ... Progress in Common Bean Breeding for Angular

of 54

  • date post

    08-Jul-2020
  • Category

    Documents

  • view

    1
  • download

    0

Embed Size (px)

Transcript of Progress in Common Bean Breeding for Angular Leaf Spot ... Progress in Common Bean Breeding for...

  • Progress in Common Bean Breeding for Angular Leaf Spot Resistance

    T.L.P.O. Souza1, M.C. Gonçalves-Vidigal2, B. Raatz3, C.M. Mukankusi4, A.F.B. Abreu1, L.C. Melo1, M.A. Pastor-Corrales5

    Common Bean Disease Workshop on Angular Leaf Spot and Root Rots Protea Hotel Kruger Gate, Skukuza, South Africa

    July 20-24, 2015

    1Embrapa Arroz e Feijão, Santo Antonio de Goiás, GO, Brazil; 2Universidade Estadual de Maringá, Maringá, PR, Brazil; 3CIAT, Bean Program, Cali, Colombia; 4CIAT, Bean Program,

    Kawanda, Uganda; 5ARS-USDA, Beltsville, MD, USA.

    July 20, 2015

  • Angular Leaf Spot (ALS)

    Sources: Sami; Rava; Schwartz.

  • • ALS resistance genes - Named genes - Unnamed genes

    • Major ALS resistance QTLs

    • Molecular markers linked to resistance loci

    • Genomic localization of resistance loci

    • Breeding efforts to develop ALS resistant germplasm/cultivars - Africa (CIAT and partners) - Brazil (Embrapa/UFLA/UFV and partners)

    Contents

  • ALS resistance genes Named genes

     Three independent dominant ALS resistance genes have been named, mapped and officially accepted by the BIC Genetics Committee

    Gene symbol R source Gene pool LG Reference

    Official Original

    Phg-1 Phg-1 AND 277 Andean (A) Pv01 Carvalho et al. (1998) Queiroz et al. (2004) Gonçalves-Vidigal et al. (2011)

    Phg-2 Phg-? Mexico 54 Mesoamerican (MA) Pv08 Sartorato et al. (2000) Namayanja et al. (2006) Mahuku et al. (2011)

    Phg-3 Phg-ON Ouro Negro Mesoamerican (MA) Pv04

    Corrêa et al. (2001) Faleiro et al. (2003) Gonçalves-Vidigal et al. (2013) Sanglard et al. (2013)

  • Markers linked to ALS resistance genes Named genes

    R Gene LG Linked marker

    Marker type

    Distance (cM)

    R Source Gene pool

    Reference

    Phg-1 Pv01

    OPH13490 RAPD 5.5

    AND 277 A

    Carvalho et al. (1998) SH13520 SCAR 5.6 Queiroz et al. (2004)

    CV542014450 STS 0.7 Gonçalves-Vidigal et al. (2011) TGA1.1570 STS 1.3

    Phg-2 Pv08 OPE04650 RAPD 11.8

    Mexico 54 MA Sartorato et al. (2000), Namayanja et al. (2006)

    OPN02890 RAPD 5.9 Sartorato et al. (2000) SN02890 SCAR 5.9

    Phg-3 Pv04

    OPM02460 RAPD 5.3

    Ouro Negro MA

    Corrêa et al. (2001) OPBA16 RAPD 10.4 Faleiro et al. (2003) SBA16560 SCAR 7.1 Queiroz et al. (2004) SM02460 SCAR 5.3 SF101050 SCAR 7.8 Gonçalves-Vidigal et al. (2013) g2303350 STS 0.0

  • ALS resistance genes Named genes

     AND 277 (gene Phg-1)

    • Single dominant gene (Andean gene pool) - Carvalho et al. (1998): race 63-23 - Gonçalves-Vidigal et al. (2011): race 63-23

    • Phg-1 + three dominant and independent genes (Caixeta et al. 2005): allelism tests using the races 31-17, 63-19 and 63-23

    • Phg-1 and Co-14 are tightly linked (0.0 cM) to each other on Pv01 (Gonçalves-Vidigal et al. 2011).

    • Important resistance source used in Brazil and Southern Africa (Aggarwal et al. 2004; Ragagnin et al. 2009; Costa et al. 2010; Sanglard 2010; Rocha et al. 2012; Ddamulira 2014)

  • ALS resistance genes Named genes

    Mexico 54 (gene Phg-2)

    • Single dominant gene (Mesoamerican gene pool) - Sartorato et al. (2000): race 63-19 - Namayanja et al. (2006): race 63-39

    • Three dominant and independent genes (Caixeta et al. 2005): allelism tests using the races 31-17, 63-19, 63-23 and 63-39

    • ALS R genes in Mexico 54 and BAT 332 are located in the same locus on Pv08 - Namayanja et al. (2006): race 63-39

    • Important resistance source used in Brazil and Pan-Africa (Oliveira et al. 2005; Namayanja et al. 2006; Sanglard 2010; Ddamulira 2014)

  • ALS resistance genes Named genes

     Ouro Negro (gene Phg-3)

    • Single dominant gene (Mesoamerican gene pool) - Corrêa et al. (2001): race 63-39 - Faleiro et al. (2003): races 31-55 and 63-31 - Gonçalves-Vidigal et al. (2013): race 63-39

    • Phg-3, Co-34 and Ur-14 are tightly linked to each other on Pv04 (Faleiro et al. 2000, 2003; Souza et al. 2011; Gonçalves-Vidigal et al. 2013).

    • Important resistance source used in Brazil (Ragagnin et al. 2009; Costa et al. 2010; Sanglard 2010; Rocha et al. 2012)

  • ALS resistance genes Unnamed genes

    R source R gene Gene pool

    LG Reference

    Cornell 49-242 Phg-? MA ?

    (Pv08)a

    Nietsche et al. (2000) – Single dominant gene

    Caixeta et al. (2005) – Single dominant gene

    Mahuku et al. (2011) – Single dominant gene

    Sanglard et al. (2013) – Single dominant gene

    MAR 2 Phg-? MA ?

    (Pv08)

    Ferreira et al. (2000) – Single dominant gene

    Caixeta et al. (2005) – Two dominant and independent genes

    Sanglard et al. (2013) – Single dominant gene

    BAT 332 Phg-? (Phg-22) MA ?

    (Pv08)

    Caixeta et al. (2003) – Single dominant gene

    Namayanja et al. (2006) – Single dominant gene (Phg-22): ALS R genes in Mexico 54 and BAT 332 are located in the same locus

    Sanglard et al. (2013) – Single dominant gene

    G10474 Phg-? MA ?

    (Pv08) Mahuku et al. (2004) – Single dominant gene

    Mahuku et al. (2011) – Single dominant gene

    G5686 Phg-? (PhgG5686A) Phg-? (PhgG5686B) Phg-? (PhgG5686C)

    A Pv04 Pv04 Mahuku et al. (2009) – Three dominant and complementary genes Pv09

    G10909 Phg-? (PhgG10909A)

    Phg-? (PhgG10909B) MA

    Pv04 Mahuku et al. (2011) – Two dominant and complementary genes

    Pv08 aPhysical position inferred based on allelism tests, mapping info and markers sequences/BLAST search.

  • Markers linked to ALS resistance genes Unnamed genes

    R gene LG Linked marker Marker

    type Distance

    (cM) R source

    Gene pool

    Reference

    Phg-? ?

    (Pv08) a

    OPE04650 RAPD 12.5 Cornell 49-242 MA Nietsche et al. (2000)OPN02890 RAPD 3.2

    SN02890 SCAR 3.2

    Phg-? ?

    (Pv08) OPE04500 RAPD 5.8 MAR 2 MA Ferreira et al. (2000)

    Phg-? (Phg-22) ?

    (Pv08) OPAA07950 RAPD 5.1 BAT 332 MA Caixeta et al. (2003)

    OPAO950 RAPD 5.8

    Phg-? ?

    (Pv08) PF5330 SCAR 5.0 G10474 MA

    Mahuku et al. (2004)

    (Andean gene pool)

    Phg-? (PhgG5686A) Phg-? (PhgG5686B) Phg-? (PhgG5686C)

    Pv04 Pv04

    Pv-ag004270 Pv-ctt001120 Pv-at007150

    SSR SSR SSR

    0.0 17.1 12.1

    G5686 A Mahuku et al. (2009) Pv09

    Phg-? (PhgG10909A) Pv04 Pv-gaat001164 SSR 13.0

    G10909 MA Mahuku et al. (2011) Phg-? (PhgG10909B) Pv08

    PF13310 PF9260

    SE04709

    SCAR

    SCAR

    SCAR

    4.9

    7.4

    9.9 aPhysical position inferred based on allelism tests, mapping info and markers sequences/BLAST search.

  • ALS resistance genes Unnamed genes

     Cornell 49-242 (Phg-?)

    • Single dominant gene (Mesoamerican gene pool) - Nietsche et al. (2000): race 31-17 - Caixeta et al. (2005): races 63-23 and 31-17 - Mahuku et al. (2011): race 1-7 - Sanglard et al. (2013): race 63-23

    • OPE04650, OPN02890 and SN02890 markers linked to Phg-2 present in Mexico 54 (Sartorato et al. 2000) are also linked to the unnamed ALS R gene present in Cornell 49-242 (Nietsche et al. 2000)

    • Gene position inferred based on mapping info: Pv08

    • Resistance source used in Brazil (Nietsche et al. 2000; Sanglard 2010)

  • ALS resistance genes Unnamed genes

     Cornell 49-242 (Phg-?)

    • ALS R genes in Cornell 49-242 (Phg-?) and Mexico 54 (Phg-2) are not allelic, once they segregate independently - Caixeta et al. (2005): allelism tests using the races 63-23 and 31-17

    • ALS R genes in Cornell 49-242 (Phg-?) and Ouro Negro (Phg-3) are not allelic, once they segregate independently - Sanglard et al. (2013): allelism test using the race 63-23

    • ALS R gene in AND 277 (Phg-1) is from the Andean gene pool and located on Pv01

    • Physical position suggests that the ALS R gene present in Cornell 49-242 (Phg-?) is an allele of Phg-2

    - Fine mapping (???)

    - Additional allelism tests (???)

  • MAR 2 (Phg-?)

    • Single dominant gene (Mesoamerican gene pool) - Ferreira et al. (2000): race 63-39 - Sanglard et al. (2013): race 63-23

    • Two dominant and independent genes (Caixeta et al. 2005): allelism tests using the races 63-19, 63-23 and 63-39

    • OPE04650 marker linked to Phg-2 present in Mexico 54 (Sartorato et al. 2000) is also linked to the unnamed ALS R gene present in MAR 2 (Ferreira et al. 2000)

    • Gene position inferred based on mapping info: Pv08

    • Resistance source used in Brazil (Oliveira et al. 2005; Sanglard 2010)

    ALS resistance genes Unnamed genes

  • ALS resistance genes Unnamed genes

    • Caixeta et al. (2005) - Allelism tests using the races 63-19, 63-23 and 63-39 - ALS R genes in MAR 2 (Phg-?) and Mexico 54 (Phg-2) are allelic: races 63-19 and 63-39 - ALS resistance genes in MAR 2 (Phg-?) and Mexico 54 (Phg-2) are NOT allelic: race 63-23

    • ALS R genes in MAR 2 (Phg-?) and Ouro Negro (Phg-3) are not allelic, once they segregate independently - Sanglard et al. (2013): allelism tests using the races 63-23 and 63-39

    • ALS R gene in AND 277 (Phg-1) is from the Andean gene pool and located on Pv01

    • Physical position suggests that the ALS R gene present in MAR 2 is an allele of Phg-2

    MAR 2 (Phg-?)