Products: Adipose-Derived Stem Cells
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www.cellider.com C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected] Adipose-Derived Stem Cells (ADSCs)
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Adipose-derived stem cells (ADCSs) are isolated from subcutaneous adipose tissue (a suitable reservoir of stem cells). These cells have the potential to differentiate in vitro into chondrocytes, osteoblasts and adipocytes and have shown their ability to heal wounds and regenerate tissues in clinical trials. ADSCs are isolated from a single animal and cultured until passage 1, and then cryopreserved. Post-thaw viable cells are tested for proliferation and differentiation capacity into adipocytes, osteocytes and chondrocytes.
Transcript of Products: Adipose-Derived Stem Cells
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Adipose-Derived Stem Cells (ADSCs)
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Bovine Adipose-Derived Stem Cells (bADSCs)
PRODUCT NAME PRODUCT SIZE CATALOG NUMBER
Bovine ADSCs 1 vial, 106 cells, cryopreserved BAD001F
Bovine ADSCs 1 vial, 106 cells, proliferating BAD001C
Bovine ADSCs 1 vial, 106 cells, pellet in RNA later BAD001P
Bovine ADSCs 4 µg total RNA BAD001R
Bovine ADSCs cDNA for 10 PCR rxns BAD001D
Description
Adipose-derived stem cells (ADCSs) are isolated
from subcutaneous adipose tissue (a suitable reservoir
of stem cells). These cells have the potential to
differentiate in vitro into adipocytes, chondrocytes,
and osteocytes and have shown their ability to heal
wounds and regenerate tissues in clinical trials.
ADSCs are isolated from a single animal and cultured
until passage 1 (Fig. 1), and then cryopreserved. Post-
thaw viable cells are tested for proliferation and
differentiation capacity into chondrocytes, osteocytes,
and adipocytes (Fig. 2).
Figure 2. Analysis of differentiation to chondrogenic (Alcian Blue), osteogenic (Von Kossa),
and adipogenic (Oil Red O) lineages of bovine adipose-‐derived stem cells.
Figure 1. Bovine adipose-‐derived stem cells in culture
at passage 1.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Specifications
Cell Type: adipose-derived stem cells Age: juvenile Specie: bovine Tissue: adipose tissue from subcutaneous fat Form: cryopreserved Viability: >70% Test performed: negative for mycoplasma, bacteria, and fungi Shipping Condition: dry ice Storage: liquid nitrogen temperature
Recommended thaw/culture protocol for cryopreserved Bovine Adipose-Derived Stem Cells (BAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous.
Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid
any possible contamination.
Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood.
1. Once the product is delivered, keep it in liquid nitrogen for long storage.
2. If you want to use cells immediately, pre-warm media and wipe the cryovial
with 70% ethanol prior to introduce in a laminar flow hood. Then, open the
cap carefully to remove the excess of pressure and then close it again.
3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2
minutes.
4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL
polypropylene sterile tube with 5-10 mL of pre-warmed media.
We recommend using α-MEM or DMEM-low glucose supplemented with 10%
(v/v) FBS and 1% (v/v) antibiotics.
5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to
eliminate DMSO traces.
6. Discard supernatant and resuspend the pellet in complete medium.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
7. Count cells to see cell viability by Trypan Blue staining or similar
methodology.
Bovine adipose-derived stem cells (BAD001F) contain at least 1 x 106 viable
cells.
8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and
5% CO2.
Recommended initial cell seeding is 10,000 cells/cm2.
9. To remove dead cells, replace media after 24-48 hours of plating and since
then, every 2-3 days.
10. When cells reached 80-90% of confluence, harvest cells using trypsin or
similar technique to detach them from the flask.
We recommend not using these cells for experiments and waiting until next
passage.
11. Culture into a new flask for expansion at a cell seeding density of 2,000
cells/cm2.
12. Repeat steps 10 and 11 until you use them for your experiments.
After 10-15 passages, cells can reduce their proliferation rate.
For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic,
therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Canine Adipose-Derived Stem Cells (cADSCs)
PRODUCT NAME PRODUCT SIZE CATALOG NUMBER
Canine ADSCs 1 vial, 106 cells, cryopreserved CAD001F
Canine ADSCs 1 vial, 106 cells, proliferating CAD001C
Canine ADSCs 1 vial, 106 cells, pellet in RNA later CAD001P
Canine ADSCs 4 µg total RNA CAD001R
Canine ADSCs cDNA for 10 PCR rxns CAD001D
Description
Adipose-derived stem cells (ADCSs) are isolated from
subcutaneous adipose tissue (a suitable reservoir of
stem cells). These cells have the potential to
differentiate in vitro into adipocytes, chondrocytes, and
osteocytes and have shown their ability to heal wounds
and regenerate tissues in clinical trials. ADSCs are
isolated from a single animal and cultured until passage
1 (Fig. 1), and then cryopreserved. Post-thaw viable
cells are tested for proliferation and differentiation
capacity into chondrocytes, osteocytes, and adipocytes
(Fig. 2).
Figure 4. Analysis of differentiation to chondrogenic (Alcian Blue), osteogenic (Von Kossa),
and adipogenic (Oil Red O) lineages of canine adipose-‐derived stem cells.
Figure 3. Canine adipose-‐derived stem cells in culture
at passage 1.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Specifications
Cell Type: adipose-derived stem cells Age: adult Specie: canine Tissue: adipose tissue from subcutaneous fat Form: cryopreserved Viability: >70% Test performed: negative for mycoplasma, bacteria, and fungi Shipping Condition: dry ice Storage: liquid nitrogen temperature
Recommended thaw/culture protocol for cryopreserved Canine Adipose-Derived Stem Cells (CAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous.
Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid
any possible contamination.
Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood.
1. Once the product is delivered, keep it in liquid nitrogen for long storage.
2. If you want to use cells immediately, pre-warm media and wipe the cryovial
with 70% ethanol prior to introduce in a laminar flow hood. Then, open the
cap carefully to remove the excess of pressure and then close it again.
3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2
minutes.
4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL
polypropylene sterile tube with 5-10 mL of pre-warmed media.
We recommend using α-MEM or DMEM-low glucose supplemented with 10%
(v/v) FBS and 1% (v/v) antibiotics.
5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to
eliminate DMSO traces.
6. Discard supernatant and resuspend the pellet in complete medium.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
7. Count cells to see cell viability by Trypan Blue staining or similar
methodology.
Canine adipose-derived stem cells (CAD001F) contain at least 1 x 106 viable
cells.
8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and
5% CO2.
Recommended initial cell seeding is 10,000 cells/cm2.
9. To remove dead cells, replace media after 24-48 hours of plating and since
then, every 2-3 days.
10. When cells reached 80-90% of confluence, harvest cells using trypsin or
similar technique to detach them from the flask.
We recommend not using these cells for experiments and waiting until next
passage.
11. Culture into a new flask for expansion at a cell seeding density of 2,000
cells/cm2.
12. Repeat steps 10 and 11 until you use them for your experiments.
After 10-15 passages, cells can reduce their proliferation rate.
For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic,
therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Equine Adipose-Derived Stem Cells (eADSCs)
PRODUCT NAME PRODUCT SIZE CATALOG NUMBER
Equine ADSCs 1 vial, 106 cells, cryopreserved EAD001F
Equine ADSCs 1 vial, 106 cells, proliferating EAD001C
Equine ADSCs 1 vial, 106 cells, pellet in RNA later EAD001P
Equine ADSCs 4 µg total RNA EAD001R
Equine ADSCs cDNA for 10 PCR rxns EAD001D
Description
Adipose-derived stem cells (ADCSs) are isolated from
subcutaneous adipose tissue (a suitable reservoir of
stem cells). These cells have the potential to
differentiate in vitro into adipocytes, chondrocytes, and
osteocytes and have shown their ability to heal wounds
and regenerate tissues in clinical trials. ADSCs are
isolated from a single animal and cultured until passage
1 (Fig. 1), and then cryopreserved. Post-thaw viable
cells are tested for proliferation and differentiation
capacity into chondrocytes, osteocytes, and adipocytes
(Fig. 2).
Figure 6. Analysis of differentiation to chondrogenic (Alcian Blue), osteogenic (Von Kossa),
and adipogenic (Oil Red O) lineages of equine adipose-‐derived stem cells.
Figure 5. Equine adipose-‐derived stem cells in culture
at passage 1.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Specifications
Cell Type: adipose-derived stem cells Age: juvenile Specie: equine Tissue: adipose tissue from subcutaneous fat Form: cryopreserved Viability: >70% Test performed: negative for mycoplasma, bacteria, and fungi Shipping Condition: dry ice Storage: liquid nitrogen temperature
Recommended thaw/culture protocol for cryopreserved Equine Adipose-Derived Stem Cells (EAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous.
Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid
any possible contamination.
Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood.
1. Once the product is delivered, keep it in liquid nitrogen for long storage.
2. If you want to use cells immediately, pre-warm media and wipe the cryovial
with 70% ethanol prior to introduce in a laminar flow hood. Then, open the
cap carefully to remove the excess of pressure and then close it again.
3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2
minutes.
4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL
polypropylene sterile tube with 5-10 mL of pre-warmed media.
We recommend using α-MEM or DMEM-low glucose supplemented with 10%
(v/v) FBS and 1% (v/v) antibiotics.
5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to
eliminate DMSO traces.
6. Discard supernatant and resuspend the pellet in complete medium.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
7. Count cells to see cell viability by Trypan Blue staining or similar
methodology.
Equine adipose-derived stem cells (EAD001F) contain at least 1 x 106 viable
cells.
8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and
5% CO2.
Recommended initial cell seeding is 10,000 cells/cm2.
9. To remove dead cells, replace media after 24-48 hours of plating and since
then, every 2-3 days.
10. When cells reached 80-90% of confluence, harvest cells using trypsin or
similar technique to detach them from the flask.
We recommend not using these cells for experiments and waiting until next
passage.
11. Culture into a new flask for expansion at a cell seeding density of 2,000
cells/cm2.
12. Repeat steps 10 and 11 until you use them for your experiments.
After 10-15 passages, cells can reduce their proliferation rate.
For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic,
therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Feline Adipose-Derived Stem Cells (fADSCs)
PRODUCT NAME PRODUCT SIZE CATALOG NUMBER
Feline ADSCs 1 vial, 106 cells, cryopreserved FAD001F
Feline ADSCs 1 vial, 106 cells, proliferating FAD001C
Feline ADSCs 1 vial, 106 cells, pellet in RNA later FAD001P
Feline ADSCs 4 µg total RNA FAD001R
Feline ADSCs cDNA for 10 PCR rxns FAD001D
Description
Adipose-derived stem cells (ADCSs) are isolated from
subcutaneous adipose tissue (a suitable reservoir of
stem cells). These cells have the potential to
differentiate in vitro into adipocytes, chondrocytes, and
osteocytes and have shown their ability to heal wounds
and regenerate tissues in clinical trials. ADSCs are
isolated from a single animal and cultured until passage
1 (Fig. 1), and then cryopreserved. Post-thaw viable
cells are tested for proliferation and differentiation
capacity into chondrocytes, osteocytes, and adipocytes
(Fig. 2).
Figure 8. Analysis of differentiation to chondrogenic (Alcian Blue), osteogenic (Von Kossa),
and adipogenic (Oil Red O) lineages of feline adipose-‐derived stem cells.
Figure 7. Feline adipose-‐derived stem cells in culture
at passage 1.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Specifications
Cell Type: adipose-derived stem cells Age: adult Specie: feline Tissue: adipose tissue from subcutaneous fat Form: cryopreserved Viability: >70% Test performed: negative for mycoplasma, bacteria, and fungi Shipping Condition: dry ice Storage: liquid nitrogen temperature
Recommended thaw/culture protocol for cryopreserved Feline Adipose-Derived Stem Cells (FAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous.
Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid
any possible contamination.
Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood.
1. Once the product is delivered, keep it in liquid nitrogen for long storage.
2. If you want to use cells immediately, pre-warm media and wipe the cryovial
with 70% ethanol prior to introduce in a laminar flow hood. Then, open the
cap carefully to remove the excess of pressure and then close it again.
3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2
minutes.
4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL
polypropylene sterile tube with 5-10 mL of pre-warmed media.
We recommend using α-MEM or DMEM-low glucose supplemented with 10%
(v/v) FBS and 1% (v/v) antibiotics.
5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to
eliminate DMSO traces.
6. Discard supernatant and resuspend the pellet in complete medium.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
7. Count cells to see cell viability by Trypan Blue staining or similar
methodology.
Feline adipose-derived stem cells (FAD001F) contain at least 1 x 106 viable
cells.
8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and
5% CO2.
Recommended initial cell seeding is 10,000 cells/cm2.
9. To remove dead cells, replace media after 24-48 hours of plating and since
then, every 2-3 days.
10. When cells reached 80-90% of confluence, harvest cells using trypsin or
similar technique to detach them from the flask.
We recommend not using these cells for experiments and waiting until next
passage.
11. Culture into a new flask for expansion at a cell seeding density of 2,000
cells/cm2.
12. Repeat steps 10 and 11 until you use them for your experiments.
After 10-15 passages, cells can reduce their proliferation rate.
For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic,
therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Ovine Adipose-Derived Stem Cells (oADSCs)
PRODUCT NAME PRODUCT SIZE CATALOG NUMBER
Ovine ADSCs 1 vial, 106 cells, cryopreserved OAD001F
Ovine ADSCs 1 vial, 106 cells, proliferating OAD001C
Ovine ADSCs 1 vial, 106 cells, pellet in RNA later OAD001P
Ovine ADSCs 4 µg total RNA OAD001R
Ovine ADSCs cDNA for 10 PCR rxns OAD001D
Description
Adipose-derived stem cells (ADCSs) are isolated from
subcutaneous adipose tissue (a suitable reservoir of
stem cells). These cells have the potential to
differentiate in vitro into adipocytes, chondrocytes, and
osteocytes and have shown their ability to heal wounds
and regenerate tissues in clinical trials. ADSCs are
isolated from a single animal and cultured until passage
1 (Fig. 1), and then cryopreserved. Post-thaw viable
cells are tested for proliferation and differentiation
capacity into chondrocytes, osteocytes, and adipocytes
(Fig. 2).
Figure 10. Analysis of differentiation to chondrogenic (Alcian Blue), osteogenic (Von Kossa), and adipogenic (Oil Red O) lineages of ovine adipose-‐derived stem cells.
Figure 9. Ovine adipose-‐derived stem cells in culture
at passage 1.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Specifications
Cell Type: adipose-derived stem cells Age: juvenile Specie: ovine Tissue: adipose tissue from subcutaneous fat Form: cryopreserved Viability: >70% Test performed: negative for mycoplasma, bacteria, and fungi Shipping Condition: dry ice Storage: liquid nitrogen temperature
Recommended thaw/culture protocol for cryopreserved Ovine Adipose-Derived Stem Cells (OAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous.
Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid
any possible contamination.
Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood.
1. Once the product is delivered, keep it in liquid nitrogen for long storage.
2. If you want to use cells immediately, pre-warm media and wipe the cryovial
with 70% ethanol prior to introduce in a laminar flow hood. Then, open the
cap carefully to remove the excess of pressure and then close it again.
3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2
minutes.
4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL
polypropylene sterile tube with 5-10 mL of pre-warmed media.
We recommend using α-MEM or DMEM-low glucose supplemented with 10%
(v/v) FBS and 1% (v/v) antibiotics.
5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to
eliminate DMSO traces.
6. Discard supernatant and resuspend the pellet in complete medium.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
7. Count cells to see cell viability by Trypan Blue staining or similar
methodology.
Ovine adipose-derived stem cells (OAD001F) contain at least 1 x 106 viable
cells.
8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and
5% CO2.
Recommended initial cell seeding is 10,000 cells/cm2.
9. To remove dead cells, replace media after 24-48 hours of plating and since
then, every 2-3 days.
10. When cells reached 80-90% of confluence, harvest cells using trypsin or
similar technique to detach them from the flask.
We recommend not using these cells for experiments and waiting until next
passage.
11. Culture into a new flask for expansion at a cell seeding density of 2,000
cells/cm2.
12. Repeat steps 10 and 11 until you use them for your experiments.
After 10-15 passages, cells can reduce their proliferation rate.
For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic,
therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Swine Adipose-Derived Stem Cells (sADSCs)
PRODUCT NAME PRODUCT SIZE CATALOG NUMBER
Swine ADSCs 1 vial, 106 cells, cryopreserved SAD001F
Swine ADSCs 1 vial, 106 cells, proliferating SAD001C
Swine ADSCs 1 vial, 106 cells, pellet in RNA later SAD001P
Swine ADSCs 4 µg total RNA SAD001R
Swine ADSCs cDNA for 10 PCR rxns SAD001D
Description
Adipose-derived stem cells (ADCSs) are isolated from
subcutaneous adipose tissue (a suitable reservoir of
stem cells). These cells have the potential to
differentiate in vitro into adipocytes, chondrocytes,
and osteocytes and have shown their ability to heal
wounds and regenerate tissues in clinical trials.
ADSCs are isolated from a single animal and cultured
until passage 1 (Fig. 1), and then cryopreserved. Post-
thaw viable cells are tested for proliferation and
differentiation capacity into chondrocytes, osteocytes,
and adipocytes (Fig. 2).
Figure 12. Analysis of differentiation to chondrogenic (Alcian Blue), osteogenic (Von Kossa), and adipogenic (Oil Red O) lineages of swine adipose-‐derived stem cells.
Figure 11. Swine adipose-‐derived stem cells in culture
at passage 1.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
Specifications
Cell Type: adipose-derived stem cells Age: juvenile Specie: swine Tissue: adipose tissue from subcutaneous fat Form: cryopreserved Viability: >70% Test performed: negative for mycoplasma, bacteria, and fungi Shipping Condition: dry ice Storage: liquid nitrogen temperature
Recommended thaw/culture protocol for cryopreserved Swine Adipose-Derived Stem Cells (SAD001F) CAUTION: cellular products should be handled with care. Animal derived products are potentially biohazardous.
Please, when working with this kind of products, always wear gloves and work behind a protective glass to avoid
any possible contamination.
Guidelines: to prevent contamination from any pathogen in your cultures, always use materials and solutions in sterile conditions and work in a laminar flow hood.
1. Once the product is delivered, keep it in liquid nitrogen for long storage.
2. If you want to use cells immediately, pre-warm media and wipe the cryovial
with 70% ethanol prior to introduce in a laminar flow hood. Then, open the
cap carefully to remove the excess of pressure and then close it again.
3. Introduce the bottom of the cryovial in the waterbath at 37°C during 1-2
minutes.
4. Once cells start to thaw, in a laminar flow cabinet, add vial content to a 15 mL
polypropylene sterile tube with 5-10 mL of pre-warmed media.
We recommend using α-MEM or DMEM-low glucose supplemented with 10%
(v/v) FBS and 1% (v/v) antibiotics.
5. Centrifuge cell suspension (300 x g during 5-7 min at room temperature) to
eliminate DMSO traces.
6. Discard supernatant and resuspend the pellet in complete medium.
www.cellider.com
C/Sanclemente 25, 4º. 50001 Zaragoza (Spain) - Telephone: +34 976 796 294. [email protected]
7. Count cells to see cell viability by Trypan Blue staining or similar
methodology.
Swine adipose-derived stem cells (SAD001F) contain at least 1 x 106 viable
cells.
8. Plate cells in one T25 flask and place in a humidified incubator at 37oC and
5% CO2.
Recommended initial cell seeding is 10,000 cells/cm2.
9. To remove dead cells, replace media after 24-48 hours of plating and since
then, every 2-3 days.
10. When cells reached 80-90% of confluence, harvest cells using trypsin or
similar technique to detach them from the flask.
We recommend not using these cells for experiments and waiting until next
passage.
11. Culture into a new flask for expansion at a cell seeding density of 2,000
cells/cm2.
12. Repeat steps 10 and 11 until you use them for your experiments.
After 10-15 passages, cells can reduce their proliferation rate.
For in vitro research use only. Not approved for human or veterinary use in vivo. Not approved for diagnostic,
therapeutic, or clinical applications. Please, check the certificate of analysis for detailed information about this product.
