Product Guide 2009 - hplc.eu · Steroids. Tel: +44 151 336 2266 2.5µm Fortis C18 Column Length 30...

Strength in Technology

Product Guide 2009

www.fortis-technologies.com Tel: +44 151 336 2266

Fortis PhilosophyHere at Fortis Technologies we take pride in understanding the needs of our customers, levels of technical service are of the highest standard and all of our products are developed in close consultation with customers to ensure that their needs are met.We believe in making method development as simple as possible, so our innovative phase chemistries offer the ability for resolution, efficiency, speed and sensitivity; all of the variables that are needed in today’s high speed evolving world.

If you have any questions please feel free to contact me on : [email protected]

Mark Woodruff Director

Table of Contents2.1µm Fortis C18™ 3-4

2.1µm Fortis Phenyl™ 5

2.1µm Fortis C8™ 6

2.1µm Fortis Cyano™ 7

2.5µm Fortis C18™ 8

Method Development Options 9-10

Fortis C18™ 11-14

Fortis Phenyl™ 15-16

Fortis H2o™ 17

Fortis C8™ 18

Fortis Cyano™ 19

Fortis HILIC™ 20

Fortis Pace™ 21-22

Fortis Prep™ Options 23

Guards and Filters 24

QC, Reproducibility 25

Applications 26-31


NewProducts

2.1µm Fortis™

Fortis C18™

Fortis Phenyl™

New

Pro

duct

s

2

– Ideal for Ultra High Pressure LC (UHPLC)- Available C18, Di-phenyl, C8, Cyano– Operate to 15,000psi– Fully Scalable analytical to prep

– Superior Peak Shapes- Low, Mid and High pH 1-12– Based upon Ultra Pure Silica– Fully Scalable

– Unique di-phenyl selectivity- Separate Positional Isomers– Aromatic retention and selectivity– Enhanced Polar Retention

Fortis C18pH12pH6pH1


2.1µm Fortis C18 (2)™

– High Efficiency– Robust Particles– Operate to 15,000psi– Fully Scalable to analytical and prep size

Fortis 2.1µm particles are designed to provide characteristics, which will aid in increased productivity within ultra high pressure chromatography (UHPLC). Designed to be robust, reproducible and fully scalable.Fortis 2.1µm particles will operate both on traditional LC systems, providing increased efficiency, and also on the latest UHPLC systems.

Increased Efficiency

Scalability and Sensitivity

Critically important to the analyst is the ability to have a fully scalable separation. Fortis C18 can be scaled from 2.1µm all the way through analytical 3 and 5µm particles to prep size without any change in retention profile.

By moving from a 3µm particle size to 2.1µm Fortis C18 sensitivity can be increased. In this example peak height goes up by over 27%

2.1µm Fortis C18 provides increased efficiency over 3µm and 5µm particles. This gives the opportunity to increase resolution or speed of analysis.

A wider linear flow range means that more resolution and peak capacity can be achieved for a given separation. By staying above 2µm particle size, Fortis 2.1µm C18 ensures pressure is reduced whilst no loss in efficiency is seen.

– Lower back pressure– Higher Efficiency– Greater Resolution– Increased Sensitivity

Minutes0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0

90

100

110

0

100

200

300

400

0.32

51.

3913

584

0.58

51.

2044

615

0.86

51.

0960

717

1.52

00.

9485

516

2.24

30.

9388

708 5µm Fortis C18

2.1µm Fortis C18

3µm Fortis C18

N = 180,564

N = 130,191

N = 88,708

- All columns 50x2.1mm

2.1µm Fortis C18

3µm Fortis C18

Diode ArrayRange: 4.631e+1

Minutes 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0 22.0

Diode ArrayRange: 3.628e+1

4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0 22.0

Minutes

3

Fortis 2.1µm


Method Development

Increase Speed

Greater speed can be achieved by moving to smaller particles, whilst still obtaining full baseline resolution.

2.1µm Fortis C18 will operate at higher linear flow rates whilst providing lower backpressure than sub 2µm particles.

To move to smaller particles and smaller columns, without affecting selectivity, use the scaling equations to optimise your UHPLC parameters: e-mail us for the spreadsheet,[email protected]

0

1.23

4438

31.

45

2.64

9136

11.

15

4.03

8756

51.

09

7.26

9225

01.

03

10.9

987

249

1.00

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0

3.0x2.1mm 2.1µm

Minutes 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0

0.9ml/min

3.0x2.1mm 2.1µm 0.3ml/min

150x4.6mm 5µm 1.0ml/min

Increase Resolution

Greater resolution can be achieved by maintaining the column length and diameter but moving to a smaller particle size.

2.1µm Fortis C18 will provide very high efficiency and therefore peak capacity and resolution if used with longer column lengths.Columns can be coupled in series to provide extreme resolution capability

Minutes 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0

Minutes0 1 2 3 4 5 6 7 8 9 10

0 1 2 3 4 5 6 7 8 9 10

Increase ResolutionIncrease Resolution

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0Minutes

Rs - 2.14

Rs - 2.58

100x4.6mm 5µm 1.2ml/min

100x2.1mm 2.1µm 0.2ml/min1. Cefadroxil2. Cefachlor3. Cefalexin4. Cefradine

2.1µm Fortis C18 (2) Column Length

30 50 100 150

2.1 F28-020202 F28-020302 F28-020502 F28-020702

Column Diameter 3.0 F28-030202 F28-030302 F28-030502 -

4.6 F28-050202 F28-050302 - -

4

Forti

s 2.1

µm


2.1µm Fortis Phenyl– High Efficiency– Robust Particles– Operate to 15,000psi– Fully Scalable to analytical and prep size

Fortis 2.1µm particles are designed to provide characteristics which will aid in increased productivity within ultra high pressure chromatography (UHPLC). Designed to be robust, reproducible and fully scalable.Fortis 2.1µm particles will operate both on traditional LC systems, providing increased efficiency, and also on the latest UHPLC systems.

2.1µm Fortis Phenyl(2) Column Length

30 50 100 150

2.1 F2PH-020202 F2PH-020302 F2PH-020502 F2PH-020702

Column Diameter 3.0 F2PH-030202 F2PH-030302 F2PH-030502 -

4.6 F2PH-050202 F2PH-050302 - -

2.1µm Fortis Phenyl™

– High Efficiency and Alternative Selectivity– Unique di-phenyl Chemistry– Operate to 15,000psi– Fully Scalable to analytical and prep size

Providing an alternate selectivity to traditional C18 and C8 chemistries, Fortis Phenyl’s unique di-phenyl structure allows ease of separation. Now the high efficiency of small particles can be combined with selectivity to ensure that resolution is optimised in ultra high pressure chromatography (UHPLC).Fortis 2.1µm particles will operate both on traditional LC systems, providing increased efficiency, and also on the latest UHPLC systems.

5

Fortis 2.1µm

Alternative Selectivity - High Efficiency

Column: Fortis Phenyl 50x2.1mm 2.1µ

p/n: F2PH-020302

Mobile Phase: 80:20 H2O : ACN + 0.1% Formic acid

Flow: 0.8ml/min

Temp: 25°C

Wavelength: 254nm

1. Gliclazide

Particularly adept at separating closely related species Fortis Phenyl is also able to provide retention and separation of many species type whether polar or non-polar.

Where alternate selectivity is required from C18 and C8 then Fortis Phenyl is the key choice.

– Higher Efficiencies– Greater Reproducibility– Resolution enhanced

Minutes0 1 2 3 4 5 6

0

100

200

300

400

0

100

200

300

400

0.3

82

1.4

42

16

2

0.6

85

2.0

70

0.8

43

5.1

91

44

75

9

1.6

75

1.4

96

07

30

To learn more about the unique structure and separating power of Fortis Phenyl turn to page 15.

Fortis Phenyl page 15

Anti-Diabetic


2.1µm Fortis C8– High Efficiency– Robust Particles– Operate to 15,000psi– Fully Scalable to analytical and prep size

Fortis 2.1µm particles are designed to provide characteristics which will aid in increased productivity within ultra high pressure chromatography (UHPLC). Designed to be robust, reproducible and fully scalable.Fortis 2.1µm particles will operate both on traditional LC systems, providing increased efficiency, and also on the latest UHPLC systems.

2.1µm Fortis C8(2) Column Length

30 50 100 150

2.1 F208-020202 F208-020302 F208-020502 F208-020702

Column Diameter 3.0 F208-030202 F208-030302 F208-030502 -

4.6 F208-050202 F208-050302 - -

2.1µm Fortis C8™


Fortis C8 2.1µm particles will provide an advantage where the hydrophobicity of a C18 is not required. C8 is also often used as the first choice in method development. Fortis C8 2.1µm particles are designed to provide characteristics which will aid in increased productivity within ultra high pressure chromatography (UHPLC). Designed to be robust, reproducible and fully scalable.

6

Forti

s 2.1

µm

Hydrophobicity - High Efficiency

Column: Fortis C8 50x2.1mm 2.1µ

p/n: F208-020302

Mobile Phase: A - H2O + 0.1% Formic acid B - ACN + 0.1% Formic acid

Gradient: 10 - 90% in 15min

Flow: 0.4ml/min

Temp: 20°C

Wavelength: 254nm

1. Telmisartan

Whatever the compound functionality Fortis C8 provides excellent performance. The high bonding density of C8 ligands can also provide resolving power different to that of C18 ligands.

Hydrophobicity is reduced from that of a C18 ligand, allowing steroids and other lipophilic species to be analysed more quickly.

– Higher Efficiencies– Greater Reproducibility– Reduced hydrophobicity over C18

Minutes0 2 4 6 8 10 12 14

0

200

400

600

800

1000

0

200

400

600

800

1000

1.7

17

1.5

83

95

77

2.5

47

0.5

61

21

10

4

2.7

10

1.1

52

44

35

5

4.3

03

1.5

91

31

17

10

Fortis Phenyl page 15

Hypertension


2.1µm Fortis Cyano™


Fortis Cyano 2.1µm particles are designed to provide alternative selectivity. Along with the other functionalities available on the Fortis 2.1µm particles this provides selectivity as well as increased efficiency for UHPLC analysis. Designed to be robust, reproducible and fully scalable, Fortis Cyano can be operated in normal phase as well reverse phase conditions.

2.1µm Fortis Cyano(2) Column Length

30 50 100 150

2.1 F2CN-020202 F2CN-020302 F2CN-020502 F2CN-020702

Column Diameter 3.0 F2CN-030202 F2CN-030302 F2CN-030502 -

4.6 F2CN-050202 F2CN-050302 - -

7

Fortis 2.1µm

Alternative Selectivity - High Efficiency

Column: Fortis Cyano 50x2.1mm 2.1µ

p/n: F2CN-020302

Mobile Phase: Heptane : THF : MeOH 90:5:5

Flow: 1ml/minTemp: 25°C

Wavelength: 254nm

1. Progesterone

2. 17αHydroxyprogesterone

3. 11αHydroxyprogesterone

4. Hydroxy-21-acetate

5. Cortisone

6. Prednisone

7. Prednisolone

Fortis Cyano works for all types of compounds either in normal phase or reversed phase solvents. Peak shapes and efficiency will be of the highest order.

Normal phase conditions will allow the retention of very polar analytes.


Minutes0 1 2 3 4 5 6

120

140

160

180

200

120

140

160

180

200

To learn more about the separating power of Fortis Cyano turn to page 19.

Fortis Cyano page 19

Steroids


2.5µm Fortis C18 Column Length

30 50 100 150

2.1 F18-020202 F18-020302 F18-020502 F18-020702

Column Diameter 3.0 F18-030202 F18-030302 F18-030502 F18-030702

4.6 F18-050202 F18-050302 F18-050502 F18-050702

2.5µm Fortis C18™

– Over 20% more efficiency than 3µm– Lower backpressure than UHPLC columns– Operate in 400bar or 1000bar systems– Fully Scalable to analytical and prep size

Fortis 2.5µm particles are designed to be the next step in improving both resolution and speed. Allowing the analyst to move towards ultra high pressure chromatography (UHPLC) whilst still operating on traditional 400bar LC systems. Combined with the companies innovative PACE™ column design, 2.5µm particles offer speed and efficiency without compromising loadability and scalability. The optimised C18 bonding maintains the phases ability to be stable from pH 1-12.

8

Forti

s 2.5

µm

Optimised Resolution

Column: Fortis C18 100x4.6mm 2.5µ

p/n: F18-050502



Flow: 1ml/min

Temp: 20°C

Wavelength: 254nm

1. Protriptyline

2. Nortriptyline

3. Amitriptyline

4. Trimipramine

Resolution of closely related species can be achieved by the use of 2.5µm particles and the optimised peak shapes afforded by Fortis C18 stationary phase.Basic, Acidic and Neutral analyte performance is first class across the pH spectrum.


Minutes0 1 2 3 4 5 6 7 8 9 10

Fortis Cyano page 19

To learn more about the stability and peak shapes of Fortis C18 turn to page 11.

Fortis C18 page 11

1.0e6

1.5e6

2.0e6

2.5e6

3.0e6

3.5e6

4.0e6

4.5e6

5.0e6

5.5e6

0.0

Tricyclic antidepressants


Fortis C18™

- General HPLC use- Method dev. from pH 1-12- Acids, Bases and Neutral

Fortis Phenyl™

- Unique di-phenyl structure- Separate Positional Isomers- Metabolite profiling

Fortis H2o™

- Polar endcapped C18- Increased polar retention- Organic acids- Catecholamines

Fortis C8™

- Reduced Hydrophobicity- Lipids- Steroids

Fortis Cyano™

- Cyano functionality- RP or NP use- Explosives- Pesticides

Fortis HILIC™

- High Polar Retention- Highly Pure Silica- Carboxylic acids - Nucleotides

Fortis Method Development Options– Choice of Stationary phase functionality– Based on Ultra pure silica– Reversed Phase (RP) and Normal Phase (NP) options

9

Method D

ev


Fortis Phenyl™

- Unique di-phenyl structure- Separate Positional Isomers- Metabolite profiling

Fortis C8™

- Reduced Hydrophobicity- Lipids- Steroids

10

Met

hod

Dev

Minutes0 1 2 3 4 5 6 7 8 9 10

mA

U

0

50

100

150

200

250

mA

U

0

50

100

150

200

250

0.04

20

.00

0

0.1

981.

05

197

0.9

601.

323

1901

1.85

81.

046

9867

2.78

80.

986

9056

4.9

800.

967

6211

6.53

31.

271

3662

7.4

98

0.9

774

047

M inutes0 1 2 3 4 5 6 7 8 9 10

mA

U

0

50

100

150

200

250m

AU

0

50

100

150

200

250

0.88

81.

262

3337

1.59

71.

125

7634

2.4

601.

066

3404

4.1

451

.02

8057

1

6.23

81.

018

3630

M inutes0 1 2 3 4 5 6 7 8 9 10

mA

U

0

50

100

150

200

250

mA

U

0

50

100

150

200

250

0.0

43

1.0

749

9

1.0

83

1.2

630

145

1.97

51

.12

6333

0

2.5

271.

145

8531

3.40

21.

037

6994

4.3

371

.01

8026

8

Minutes0 1 2 3 4 5 6 7 8 9 10

mAU

0

50

100

150

200

250

0

50

100

150

200

250

Minutes0 1 2 3 4 5 6 7 8 9 10

mA

U

0

50

100

150

200

250

mA

U

0

50

100

150

200

250

0.9

63

1.3

324

885

1.8

701

.19

6902

5

2.70

51.

147

3634

4.3

881.

08

888

78

6.1

171

.05

8907

9

Fortis C8™

Fortis C18™

Fortis Phenyl™Fortis H2o™

Fortis Cyano™

Getting Started :Method development typically starts with a C18 or C8 column, both provide Hydrophobic retention with good peak shapes for neutral, acidic and basic analytes. Generally if retention of polar molecules is also needed then a polar endcapped stationary phase such as Fortis H2o is a good starting choice.If selectivity is insufficient then Phenyl or Cyano stationary phases are a good alternative, they will change selectivity and even elution order since they work on dipole characteristics as opposed to just hydrophobicity.Fortis Cyano is good in normal phase (NP) conditions for polar analytes with COOH, NH2, NHR2 or NR2 groups. If small polar molecules still do not retain then HILIC chromatography is a suitable alternative.

Acidic, Neutral & basic analytes- Fortis C18- Fortis C8- Fortis Phenyl

Polar basic molecules- Fortis C18 operated at high pH- Fortis Phenyl- Fortis H2o

Polar acidic molecules- Fortis H2o- Fortis HILIC- Fortis Cyano in NP mode

Alternate Selectivity- Fortis Phenyl- Fortis Cyano

Extra PolarRetention

ExtraSelectivity

LowerHydrophobicity

ChangeSelectivity


Fortis C18™

– Superior Peak Shapes– pH Range 1-12– Based on Ultra Pure Silica – Fully Scalable

Fortis C18 is a pure silica based stationary phase with unique high and low pH performance. Whether carrying out simple compound screens or complex metabolite identification Fortis C18 will provide the best in peak shape, resolution and extended pH range for method development flexibility.

Optimised Peak Shape

L1380m

2/g

17%

C18

2.5µ

3µ

5µ 1

0µ

Column: Fortis C18 100x4.6mm 3µ

p/n: F18-050503



Flow: 1ml/min

Temp: 20°C

Wavelength: 254nm

1. Protriptyline

2. Nortriptyline

3. Amitriptyline

4. Trimipramine

Whatever the compound functionality the optimised hydrophobic bonding of Fortis C18 leads to peak symmetries being near perfect whatever the analyte type.

Basic, Acidic and Neutral analyte performance is first class across the pH spectrum.

– Superior Peak Shapes- Higher Efficiencies– Greater Reproducibility

Minutes0 1 2 3 4 5 6 7 8 9 10

Extreme pH range

Fortis C18 has the ability to not only operate at low pH like other silica based phases, but also to operate at high pH to aid with basic analyte retention and performance.

The ability to quickly equilibrate from formic acid or TFA into ammonia or bicarbonate aids in method development. Mass transfer, loadability and precision of a silica matrix are all maintained.

– Higher Efficiency than Hybrids- Greater Reproducibility– Retain polar basic analytes

High pH ComparisonHigh pH Comparison

50

100

150

200

250

300

350

Minutes0 2 4 6 8 10 12 1450

100

150

200

250

300

350

Columns: 150x4.6mm 3μ Mobile Phase: 50:50 0.1% NH3 : MeCN Flow: 1.0ml/min Temp: 25°C Wavelength: 230nm

Fortis C18™

Hybrid C18

Asy - 0.97N - 112,225Rs - 2.28

Asy - 1.22N- 71,865Rs - 1.51

11

Fortis PhenylFortis C

18


Columns: 150x4.6mm 3μ Mobile Phase: 50:50 0.1% NH3 : MeCN Flow: 1.0ml/min Temp: 25°C Wavelength: 230nm

pH 1

pH 6

pH 12

0 2 4 6 8 10 12 14

-0.02

0.00

0.02

0.04

0.06

7.61

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2

Minutes

12

Forti

s Phe

nyl

Forti

s C18

Minutes0 2 4 6 8 10 12 14

Minutes0 1 2 3 4 5 6 7 8 9 10


p/n: F18-050503



Flow: 1ml/min

Temp: 20°C

Wavelength: 254nm

1. Dexamethasone

2. Neomycin Sulphate

3. Acetic acid


p/n: F18-050305

Mobile Phase: A - 50mM NH4OAc B - ACN


Flow: 1ml/min

Temp: 20°C

Wavelength: 254nm

1. Quetiapine


p/n: F18-030303

Mobile Phase: 30:70 H2O + 10mM ammonium bicarbonate : MeOH

Flow: 0.4ml/min

Temp: 25°C

Wavelength: MS Detection

Raloxifene Glucuronides

Data Courtesy of : Pharmaceutical company, USA

Extended pH RangeInflammation and Infection

Antipsychotic

Selective Estrogen Modulator


Optimised Resolution

Only by optimising all factors of stationary phase design can the analyst be assured of the best possible chromatography.

Fortis C18’s unique bonded character ensures that not only is reproducibility and robustness assured, but also that resolution is of the highest level. Only by obtaining sharp peak shapes for many analyte types can this sort of resolution be achieved.

Analysed here are 135 transitions of pesticide residues. Good LC resolution leads to excellent sensitivity in MS detection.

XIC of +MRM (135 pairs): 184.0/143.0 amu from Sample 17 (Coltest_017) of DataColtest.wiff (Turbo Spray) Max. 9.2e4 cps.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24Time, min

0.0

5.0e4

1.0e5

1.5e5

2.0e5

2.5e5

3.0e5

3.5e5

4.0e5

4.5e5

5.0e5

5.5e5

6.0e5

3.16

7.696.064.822.85

Minutes0 5 10 15 20 25

Data Courtesy of : Central Science Laboratories, UK

Column: Fortis C18 150x2.1mm 3μ

13

Fortis C18

High pH Stability

The unique bonding of Fortis C18 enables stability at extremes of pH to be maintained.

Run continuously in 0.1% ammonia Fortis C18 shows no deterioration in efficiency over a 90 day period.

Fortis C18™

135 Pesticide Transitions


Fortis C18 Guards Length

10

Column Diameter 2.1 F18-0200xxG

4.6 F18-0500xxG

Fortis C18 Column Length

50 100 150 250

2.1 F18-0203xx F18-0205xx F18-0207xx -

Column Diameter 3.0 F18-0303xx F18-0305xx F18-0307xx -

4.6 F18-0503xx F18-0505xx F18-0507xx F18-0509xx

Replace xx - 02 for 2.5µm - 03 for 3µm - 05 for 5µm - 10 for 10µm

Hydrophobicity

Fortis C18 high surface area combined with the optimised C18 ligand bonding provides high retention for compounds.This is advantageous in a number of ways:

- Higher retention of analytes, more organic modifier can be used to elute, therefore greater MS sensitivity.

- Higher retention of analytes, more organic leads to shorter ‘dry-down’ in fraction collection.

- Higher retention of analytes, more chance of resolution

9.75 10.00 10.25 10.50 10.75 11.00 11.25 11.50 11.75 12.00

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

208.15 (1.00)194.15 (1.00)180.15 (2.51)150.15 (1.00)136.15 (1.83)

MD

EA

/11.2

00

MD

MA

Ecsta

sy/1

0.6

67

MD

A/1

0.0

00

Me

tha

mpheta

min

e/1

0.9

17

Am

pheta

min

e/1

0.2

17

10 10.5 11.0 11.5 12.0

Minutes

14

Forti

s C18


p/n: F18-030703

Mobile Phase: A - H2O + 0.1% ammonia B - MeOH + 0.1% ammonia

Gradient:

Flow: 0.4ml/min

Temp: 20°C


1. MDA (3,4-Methylenedioxyamphetamine)

2. Amphetamine

3. MDMA (Ecstasy)

4. Methaphetamine

5. MDEA (3,4-Methylenedioxy-N-ethylamphetamine)

Drugs of Abuse

Data Courtesy of : Forensic Laboratory, UK

Fortis C18™


Fortis Phenyl™

– Unique Selectivity– Separate Positional Isomers– No “MS bleed”, stable hydrophobic ligand– Enhanced Polar Retention

Fortis Phenyl is designed to provide characteristics which will enhance selectivity. It provides the analyst with extra retention of compounds containing aromatic functionality. Extra selectivity and retention can be found for polar substrates, along with metabolite profiling.

Unique Functionality

Fortis Phenyl is based upon a unique di-phenyl functionality. Three controlled mechanisms of interaction can occur.

This allows for unique resolution of closely related species, and metabolites. No complex mobile phases are necessary simplifying method development.

– π-π High Selectivity– Resolution enhanced– Sharp Peak Shapes- Highly Stable phenyl ligand

L11380m

2/g

13%

Diphenyl

2.5µ

3µ

5µ 1

0µ

Separate Positional Isomers

Column: Fortis Phenyl 150x4.6mm 5µ

p/n: FPH-050705

Mobile Phase: 40:60 H2O : MeOH

Flow: 1ml/min

Temp: 20°C

Wavelength: 210nm

1. 4-Hydroxyestradiol (mw=288.38)

2. 2-Hydroxyestradiol (mw=288.38)

Selectivity of compounds normally difficult to resolve on a hydrophobic alkyl chain stationary phase is simplified by the π-π interactions provided by the phenyl functionality.

In this application two hydroxyestradiol steroids exhibit resolution from each other, which is not achievable on alkyl chain phases. No complex mobile phases are necessary.

– High Selectivity– Resolution enhanced– Sharp Peak Shapes

Minutes

50

75

100

125

150

175

200

50

75

100

125

150

175

200OH

OH

CH3

OH

H

H

H

OH

CH3

OH

H

H

H

OH

0 2 4 6 8 10 12 14

15

Fortis Phenyl

Hydrophobicity(alkyl chain)

Steric Selectivity(diphenyl arrangement)

π-π(phenyl rings)


Fortis Phenyl Guards Length

10

Column Diameter 2.1 FPH-0200xxG

4.6 FPH-0500xxG

Fortis Phenyl Column Length

50 100 150 250

2.1 FPH-0203xx FPH-0205xx FPH-0207xx -

Column Diameter 3.0 FPH-0303xx FPH-0305xx FPH-0307xx -

4.6 FPH-0503xx FPH-0505xx FPH-0507xx FPH-0509xx

Replace xx - 02 for 2.5µm - 03 for 3µm - 05 for 5µm - 10 for 10µm

PET Tracer - PK11195


p/n: FPH-050705

Mobile Phase: 40 : 60 H2O : ACN

Flow: 1ml/min

Temp: 25°C


1. Dechlorinated PK111952. PK11195

Antiarrhythmic


p/n: FPH-050705

Mobile Phase: 70 : 30 H2O + 0.1% formic acid MeOH

Flow: 1ml/min

Temp: 25°C

Wavelength: 235nm

1. Quinidine

2. Dihydroquinidine• Column : • p/n : • Mobile Phase:

• Flow : • Temp : •

0.0 2.5 5.0 7.5 10.0 12.5 15.0

319.00 (1.00)353.00 (1.00)

Minutes

• Column : • p/n : • Mobile Phase:


Minutes0 2 4 6 8 10 12 14 16 18 20

60

80

100

120

140

160

180

Minutes

0 2.5 5.0 7.5 10 12.5 15

Data Courtesy of : Wolfson Molecular Imaging Centre

353.00 (1.00)319.00 (1.00)

0 2 4 6 8 10 12 14 16 18 20

16

Forti

s Phe

nyl

2.1µm Fortis Phenyl page 5

Di-phenyl vs C18

Selectivity of the Fortis di-phenyl is radically different to that of a C18 stationary phase.

In this pharmaceutical mixture we can see an increase in retention of the parent drug, whilst the degradents are all eluted quickly, removing them from co-elution with the parent.

Selectivity such as this can be extremely useful, combined with the ability to separate closely related species such as metabolites and positional isomers.

Minutes

50

75

100

125

150

14

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fortis™ Phenyl

Fortis™ C18

Data Courtesy of : Major Pharmaceutical company, USA


0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 3.00 3.25 3.50 3.75

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

(x1,000)

73.00 (1.00)91.00 (1.00)117.00 (1.00)

0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 3.00 3.25 3.50 3.75

0.25

0.50

0.75

1.00

1.25

1.50

1.75

2.00

(x10,000)

73.00 (1.00)91.00 (1.00)117.00 (1.00)

Fortis H2o’s unique bonded character ensures that not only is reproducibility and robustness assured, but also that resolution is of the highest level.

Fortis H2o is optimised not only to help retain and resolve polar analytes, but also to be complimentary in resolution to Fortis C18.

– Hydrophilic as well as Hydrophobic interaction’s– Alternative Selectivity– No MS bleed

Fortis H2o™

– Retention of Polars– Enhanced Resolution– 100% Aqueous Compatible– Fully Scalable

Fortis H2o is designed to aid in the separation and retention of polar analytes. Complex mobile phase systems can be bypassed if sufficient retention can be provided by the stationary phase chemistry. Fortis H2o is designed to supply additional interaction with polar molecules which allows their successful retention.

Alternative Selectivity - Organic acids

Retention of Polar analytes

Column : Fortis H2o 150x4.6mm 5µ

p/n : FHO-050705

Mobile Phase: H2O + 0.1% Formic acid

Flow : 0.8ml/min

Temp : 20°C

Wavelength: 270nm

1. Dopamine

2. Serotonin

3. DOPAC

4. 5-HIAA

Column: Fortis H2o 150x2.1mm 3µ

p/n: FHO-020703

Mobile Phase: 5mM Ammonium Formate + 0.5% Formic acid

Flow: 0.4ml/min

Temp: 60°C


Minutes

L1380m

2/g

18%

3µ 5

µ 10

µ

Polar Endcapping

0 1 2 3 4 5 6 7 8 9 10

17

Fortis H2o

Methylmalonic acid

Succinic acid

Neurotransmitters


Fortis C8™

– Reduced Hydrophobicity over C18– Excellent Peak Shapes– Fully Scalable

Fortis C8 is designed to provide characteristics similar to Fortis C18 but specifically for situations where less hydrophobicity is required. The same gains in peak shape, efficiency, resolution and scalability are available providing increased productivity to the analyst.

Optimised Peak Shape

L7380m

2/g

13%

C8

2.1µ

3µ

5µ 1

0µ


p/n: F08-050705

Mobile Phase: A - H2O + 0.1% Formic acid B - MeOH + 0.1% Formic acid


Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

1. Lamotrigine

Fortis C8 is optimised to provide the best possible peak shapes and efficiency.

Basic, Acidic and Neutral analyte performance is first class.

– Higher Efficiencies– Greater Reproducibility– Symmetrical peak shapes- Lower Hydrophobicity

Minutes0 1 2 3 4 5 6 7 8 9 10

Minutes0 1 2 3 4 5 6 7 8 9 10

200

400

600

800

1000

Minutes0 1 2 3 4 5 6 7 8 9 10

100

150

200

250

300

Minutes0 1 2 3 4 5 6 7 8 9 10

Anticonvulsant

Minutes0 1 2 3 4 5 6 7 8 9 10 11 12

60

80

100

120

140

160

1.39

80.

7829

51

2.66

21.

2158

942

6.08

81.

1686

411

Minutes 0 1 2 3 4 5 6 7 8 9 10 11 12

Antiplatelet


p/n: F08-050705

Mobile Phase: 40 : 60 H2O + 0.1% formic acid ACN


Wavelength: 220nm

1. Valproate Semisodium


p/n: F08-050705

Mobile Phase: 25 : 75 H2O : ACN


Wavelength: 254nm

1. Clopidogrel

18

Forti

s C8

Bipolar disorders


Fortis Cyano™

– Retention of Polars– Alternative Selectivity– Normal Phase or Reverse Phase system– Rapid Equilibration

Fortis Cyano allows the use of aqueous reversed phase conditions to provide less retention for compounds too heavily retained on C18 functionality. However, it can also be used in normal phase solvent systems to retain and separate polar analyte species.Cyano columns are particularly useful for polar species, Fortis Cyano is now also available in 2.1µm particle size for UHPLC work.

L10380m

2/g

7%

2.1µ

3µ

5µ

Cyano

Fortis Cyano Guards Length

10

Column Diameter 2.1 FCN-0200xxG

4.6 FCN-0500xxG

Fortis Cyano Column Length

50 100 150 250

2.1 FCN-0203xx FCN-0205xx FCN-0207xx -

Column Diameter 3.0 FCN-0303xx FCN-0305xx FCN-0307xx -

4.6 FCN-0503xx FCN-0505xx FCN-0507xx F18-0509xx

Replace xx - 02 for 2.1µm - 03 for 3µm - 05 for 5µm

19

Fortis Cyano

Fortis Cyano is optimised not only to help retain and resolve polar analytes, but also to be complimentary in resolution to other Fortis phases.

– Normal phase as well as Reversed phase use– Alternative Selectivity– Rapid Equilibration

Herbicides

Column : Fortis Cyano 50x2.1mm 3µ

p/n : FCN-020303

Mobile Phase: 80:20 H2O : ACN + 0.2% Acetic acid

Flow : 0.2ml/min

Temp : 20°C

Wavelength: 280nm

1. Banvel

2. Int Std

3. 2,4-D

4. MCPA

5. PCOC

6. 2,4-DCP

7. 2,4-DP

8. CMPP

9. 2,4-DB

10. MCPB

Minutes

0

50

100

150

200

250

300

350

0

50

100

150

200

250

300

350

0.6

77

1.2

03

74

44

1.4

70

0.7

43

30

24

1.7

70

1.3

41

61

06

4

2.0

00

1.5

80

3.0

13

1.0

22

18

29

8

3.2

17

1.2

43

36

50

4

4.6

28

0.8

51

93

67

1

5.2

42

0.9

12

28

95

2

5.8

58

0.9

12

60

62

0

6.2

10

1.0

92

72

05

4

6.7

53

0.9

32

33

99

9

7.5

35

0.9

42

50

18

3

12

.9

80

0.0

02

12

99

5

13

.5

63

0.0

02

33

48

9

0 2 4 6 8 10 12 14

To see more applications on Fortis Cyano turn to page 26. To learn more about Fortis Cyano 2.1µm see page 7.

Herbicides


Fortis HILIC™

– Retention of Polars– Increased MS sensitivity– Alternate selectivity– Reduced extraction (SPE) and dry down times.

Fortis HILIC (Hydrophilic Interaction Chromatography) is designed to aid in the separation and retention of very polar analytes. Extended retention is afforded by the partitioning, ion-exchange and hydrogen bonding that can occur on a HILIC stationary phase. Fortis HILIC can increase sensitivity in MS analysis and provide alternate selectivity to that achieved with reversed phase C18.

L3380m

2/g

na

3µ, 5

µ

HILIC

Fortis HILIC Guards Length

10

Column Diameter 2.1 FHI-0200xxG

4.6 FHI-0500xxG

Fortis HILIC Column Length

50 100 150 250

2.1 FHI-0203xx FHI-0205xx FHI-0207xx -

Column Diameter 3.0 FHI-0303xx FHI-0305xx FHI-0307xx -

4.6 FHI-0503xx FHI-0505xx FHI-0507xx FHI-0509xx

Replace xx - 03 for 3µm - 05 for 5µm - 10 for 10µm

20

Forti

s HIL

IC

Fortis HILIC is optimised to help retain and resolve polar analytes. By use of high concentrations of organic solvent polar analytes partition with the stationary phase.

– Polar Retention– Alternative Selectivity– Rapid Equilibration

Nucleosides

Column : Fortis HILIC 50x4.6mm 5µ

p/n : FHI-050305

Mobile Phase: 95:5 ACN : 100mM NH3OAc

Flow : 1ml/min

Temp : 20°C

Wavelength: 254nm

1. Uracil

2. Uridine

3. Cytosine

4. Guanosine

Minutes

10

20

30

40

10

20

30

400

.6

10

.85

14

00

1.2

10

.8

52

21

7

4.8

91

.1

42

62

7

6.8

41

.18

29

85

0 1 2 3 4 5 6 7 8 9 10

Hydrophilic Interaction Chromatography (HILIC) works in a similar way to normal phase chromatography. A polar surface combined with a non-polar mobile phase, typically ACN, allows for partition of the polar analytes and hence retention and separation. Water is used in low concentration as the strong solvent in order to elute the compounds.

60 70 80 90% organic

Adenine

Theobromine

Uracil

Toluene8

6

4

2

10

k’

Nucleoside’s


Fortis – LC-MS Optimised Hardware– 20mm and 30mm lengths- High Throughput– High Efficiency and Resolution

Fortis Pace™ columns are designed with High Throughput Screening (HTS) applications in mind. Optimised for use in LC-MS to provide greatest sensitivity by achieving sharp peak shapes combined with excellent resolution and retention. Any Fortis stationary phase can be supplied in this hardware.

Optimised Hardware

Fortis stationary phases have been proven to exhibit excellent peak shapes and efficiency, packed in Pace hardware allows speed and resolution to be achieved without the need for UHPLC systems.

Providing highly retentive and selective phases allows strong retention properties, enabling high concentrations of organic modifier to be utilised optimising the MS ionisation process.

Gains are also made in:

- Reduced analysis time- Increased productivity- Lower solvent consumption

Column: Fortis Pace C18 30x2.1mm 5µ

p/n: F18-020205



Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

1. Alkyl Benzenes


p/n: F18-020205

Mobile Phase: 75:25 H2O:ACN + 0.1% Formic acid

Flow: 0.4ml/min

Temp: 25°C

Wavelength: 254nm

1. Rosuvastatin

2. Atorvastatin

Fortis Pace column hardware is specifically designed for HTS, whether isocratic or by ballistic gradients. Optimised packing density in this low volume hardware leads to ultra sharp peak shapes combined with maximum efficiency.

– Reduced peak widths– Higher Efficiency– Resolution enhanced

Minutes 0.0 0.5 1.0 1.5 2.0 2.5 3.0

Minutes0.0 0.2 0.4 0.6 0.8

Complimentary Stationary Phases


Alkyl Benzenes• Column : Fortis C18 30x2.1mm 5• p/n : F18-020205• Mobile Phase:

A – H2O + 0.1% Formic acidB – ACN + 0.1% Formic acid

• Gradient: 60 – 90% in 2min

• Flow : 1 ml/min• Temp : 25oC• : 254nm

1. Alkyl Benzenes

Minutes0.0 0.5 1.0 1.5 2.0 2.5 3.0

Vol

ts

Vol

ts

0.000

0.002

0.004

0.006

0.008ACS


Alkyl Benzenes• Column : Fortis C18 30x2.1mm 5• p/n : F18-020205• Mobile Phase:

A – H2O + 0.1% Formic acidB – ACN + 0.1% Formic acid

• Gradient: 60 – 90% in 2min

• Flow : 1 ml/min• Temp : 25oC• : 254nm

1. Alkyl Benzenes

Minutes0.0 0.5 1.0 1.5 2.0 2.5 3.0

Vol

ts

Vol

ts

0.000

0.002

0.004

0.006

0.008ACS


Statins• Column : Fortis C18 30x2.1mm 5• p/n : F18-020205• Mobile Phase:

75:25 ACN:H20+ 0.1% Formic acid

• Flow : 0.4 ml/min• Temp : 25oC• : 254nm

1. Rosuvastatin2. Atorvastatin

Minutes0.0 0.2 0.4 0.6 0.8

0.18

71.3

7671

9

Retention TimeAsymmetry (10%)Theoretical plates / meter

21

Fortis PaceStatins


Applications

SSRI Antidepressant

Column: Fortis Pace H2o 30x2.1mm 3µ

p/n: FHO-020203


Flow: 0.6ml/min

Temp: 25°C

Wavelength: 200nm

1. Paroxetine

Benzodiazepines


p/n: FHO-020203

Mobile Phase: 50:50 ACN : H2O

Flow: 0.7ml/min

Temp: 25°C

Wavelength: 220nm

1. Lorazepam

2. Temazepam

3. Diazepam

Antidepressant

1. Mirtazapine

Sulfa Drugs


p/n: FHO-020203


Flow: 0.2ml/min

Temp: 25°C

Wavelength: 254nm

1. Sulfathiazole

2. Sulfamerazine

3. Sulfamethoxazole


p/n: F18-020205

Mobile Phase: 80:20 H2O : MeOH + 0.1% Formic acid

Flow: 0.7ml/min

Temp: 25°C

Wavelength: 254nm


Paroxetine• Column : Fortis H2o 30x2.1mm 3• p/n : FHO-020203• Mobile Phase:

70:30 H2O:ACN+ 0.1% Formic acid


1. Paroxetine

Minutes0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0

0

2

4

6

0.06

70.0

00

0.66

21.4

5 473

03


Minutes


Mirtazepine• Column : Fortis C18 30x2.1mm 5• p/n : F18-020205• Mobile Phase:

80:20 H20 : MeOH+0.1% Formic acid


1. Mirtazepine

Minutes0.0 0.5 1.0 1.5 2.0 2.5 3.0

Vol

ts

0.000

Vol

ts

0.000

0.001

0.002

0.003

1.55

0 ACS

Minutes


Sulfa Drugs• Column : Fortis H2o 30x2.1mm 3• p/n : FHO-020203• Mobile Phase:

70:30 H20 : ACN+0.1% Formic acid


1. Sulfathiazole2. Sulfamerazine3. Sulfamethoxazole

Minutes0.0 0.5 1.0 1.5 2.0 2.5 3.0

Vol

ts

0.00

0.01

0.02

0.03

0.04

0.44

81.4

9 244

44

0.56

01.5

9345

01

1.06

01.3

8669

79


Minutes


Benzodiazepines• Column : Fortis H2o 30x2.1mm 3• p/n : FHO-020203• Mobile Phase:

50:50 ACN:H20


1. Lorazepam2. Temazepam3. Diazepam

Minutes0.2 0.6 1.0 1.4 1.8

Vol

ts

0.00

0.01

0.02

0.03

0.04

2049

5052

2.6

10.2

ACS30x2-1 3um 07ml min Run3

Area

Minutes 0.2 0.6 1.0 1.4 1.8 0.2 0.6 1.0 1.4 1.8

0.0 0.5 1.0 1.5 2.0 2.5 3.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0

Fortis Pace 3µ Column Length

20 30

2.1 Fxx-020103 Fxx-020203

Column Diameter 3.0 - Fxx-030203

4.6 Fxx-050203

Fortis Pace 5µ Column Length

20 30

2.1 Fxx-020105 Fxx-020205

Column Diameter 3.0 - Fxx-030205

4.6 - Fxx-050205

Replace xx with: 18 for Fortis C18; OH for Fortis H2o; PH for Fortis Phenyl; 08 for Fortis C8; CN for Fortis Cyano;

22

Forti

s Pac

e


Fortis Prep

Fortis Prep™

– 5µm and 10µm particles– High Loadability– Optimised Packing Efficiency– Narrow peak profile, High Efficiency and Resolution

Fortis Prep columns are designed for high sample loading, high throughput applications. The optimised packed bed (OPB) process ensures excellent peak shapes and efficiency, whilst the lifetime of the column is increased.

Columns & BulkFortis Prep columns come in sizes from 30mm to 250mm in length and from 10mm in diameter all the way up to 50mm (2” i.d.).Pre-packed columns are advised for < 2” i.d. after this Bulk material can be supplied for those wishing to pack DAC (Dynamic Axial Compression) columns.

If preparative columns are packed with the identical media to their analytical counterpart then the ability to scale up with the theoretical calculations will be accurate.

- 10mm, 21.2mm, 30mm and 50mm i.d.- 5µm and 10µm particles- 100g to multi Kg bulk available

Contact us for more information on availability of prep options/bulk packings, or to discuss your application and the ability to scale up. Our technical experts will be happy to discuss your needs with you.

www.fortis-technologies.com Tel: +44 151 336 2266 24

Forti

s Gua

rds

Filter & Guard Options– Guard system for all 3µm, 5µm and 10µm phases– Low volume in-line filters for LC and UHPLC– Maintain chromatographic integrity

Fortis Guards and filters are designed to ensure that erroneous materials do not find there way onto the more important and expensive analytical column. Guards are available in sizes to match all analytical and preparative column dimensions. Filters are particularly suitable for short fast LC/MS (Pace™) columns and UHPLC columns.

– Guard system for all 3µm, 5µm and 10µm phases– Quick replacement cartridges- Highly Cost Effective

– Preparative guard system 10mm & 21.2mm– Quick replacement cartridges- Highly Cost Effective

– Filter for all LC columns– Low volume in-line filters– Change over time - seconds not minutes

Fortis in-line filters are fingertight direct connect design, fitting in between the column and the conventional peek fitting to filter out particulate matter, it contains low dead volume and pressure. In-line filters are ideal for very short fast columns such as Fortis Pace™ LC/MS columns where extra packed bed from a guard would be detrimental. In-line filters are also available in UHPLC format, capable of withstanding the elevated pressures involved.

3µm Fortis C18 Cartridges

F18-040003G/2 10x4mm Fortis C18 3µm Guard pk 2




5µm Fortis C18 Cartridges





In-line Filters

2-SAV5 2µm In-line filter pk 5

2-SAV10 2µm In-line filter pk 10

UHPSAV2 UHPLC In-line filter pk 2

UHPSAV4 UHPLC In-line filter pk 4


Fortis QC

QC Reproducibility– Robust column bondings– Assured Peak shapes– 20% lower asymmetry specification– 10% Higher Efficiency

Fortis HPLC columns are tested using the industries most rigorous QC test, utilising basic analyte probes as well as neutral efficiency markers ensures that the column reproducibility is first class. Fortis columns are also subject to a 20% lower peak shape specification than other manufacturers columns.

Fortis stationary phases have been proven to exhibit excellent peak shapes and efficiency for the full range of analyte species.

By employing a QC mix that accurately probes silanol activity (the measure of good peak shape) the analyst can be assured of quality time and time again.

Gains are also made in:

- Sample carry over- Increased Resolution- Increased Sensitivity


p/n: F18-050505

Mobile Phase: 60:40 ACN:H2O

Flow: 1.0ml/min

Temp: 25°C

Wavelength: 254nm

1. Uracil

2. Phenol

3. 4-Ethylaniline

4. N,N-Dimethylaniline

5. Napthalene

Minutes0 1 2 3 4 5 6 7 8 9 10

QC Test

0 1 2 3 4 5 6 7 8 9 100.0

0.1

0.2

0.3

0.4

0.5

0.0

0.1

0.2

0.3

0.4

0.5

0.84

813

618

1.10

1.67

878

110

1.20

2.54

591

605

1.11

4.63

010

7875

1.04

7.00

210

4571

1.03

QC HP1050

Fortis columns are subject to tight specification using basic analytes in an unbuffered mobile phase system.If there were residual uncovered hydroxyl groups present then these basic probes would highlight this fact.

Fortis Technologies unique bondings combined with the ultra pure silica matrix ensure that the peak shapes achieved are first class.

Column Asymmetry Reproducibility

Asymmetry

500+ columns


Casein Tryptic Digest

Minutes0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30

Column: 2.1µm Fortis C18(2) 100x2.1mm

p/n: F28-020502


Gradient: 0min 5% B

5min 5% B

30min 20% B

35min 50% B

Flow: 0.25 ml/min

Temp: 35°C

Wavelength: 200nm

1. Casein

Antipsychotic


p/n: FPH-050705

Mobile Phase: 35:65 H2O + 0.1% Formic acid : MeOH

Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

1. Prochlorperazine Maleate

Antimalarial


p/n: FPH-050305

Mobile Phase: A: H2O + 0.1% formic acid B: MeOH + 0.1% formic acidGradient: 10 - 90% in 10mins

Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

1. Proguanil

Stemetil• Column : • p/n : • Mobile Phase:


Minutes0 1 2 3 4 5 6 7 8 9 10

50

60

70

80

90

Minutes

Paludrine• Column : • p/n : • Mobile Phase:

• Gradient:


Minutes1 2 3 4 5 6 7 8 9 10

100

200

300

400

500

600

700

800

Minutes0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10

Applications

26

App

licat

ions


Applications

Opioid analgesic


p/n: F18-020305

Mobile Phase: A: H2O + 0.1% NH3

B: ACN + 0.1% NH3

Gradient: 10 - 100% in 5minsFlow: 0.2ml/min

Temp: Ambient

Wavelength: 220nm

1. Tramadol

Antibiotic


p/n: F18-050305

Mobile Phase: A: H2O + 0.1% formic acid B: ACN + 0.1% formic acidGradient: 10 - 100% in 10mins

Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

1. Phenoxymethylpenicilin

Vitamin

1. Vitamin C

Antibiotic


p/n: FPH-050705


Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

1. Ciprofloxacin


p/n: FPH-050705

Mobile Phase: 100% H2O + 0.1% Formic acid

Flow: 0.8ml/min

Temp: 25°C

Wavelength: 254nm

0.33

0

ACS1050 10-2-06 Run 2

Retention Time

Tramadol

Minutes

Vitamin C• Column : • p/n : • Mobile Phase:


Minutes0 1 2 3 4 5 6 7 8 9 10

0

2

4

6

8

10

12

14

Minutes

Ciprofloxacin• Column : • p/n : • Mobile Phase:

• Gradient:


Minutes0 1 2 3 4 5 6 7 8 9 10

200

400

600

800

1000

1200

6.84

3411

881.

30

8.41

00.

00

8.74

00.

00

9.06

00.

00

9.77

00.

00

Minutes

0 1 2 3 4 5 6 7 8 9 10Minutes

0 1 2 3 4 5 6 7 8 9 10 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0

0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10

27

Applications


Applications

Adrenergic alpha-antagonist


p/n: FPH-050705

Mobile Phase: 30:60 H2O + 0.1% Formic acid : MeOH

Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

1. Doxazosin

Explosives

Column: Fortis Cyano 50x2.1mm 3µ

p/n: FCN-020303

Mobile Phase: A: 80:20 H2O : MeOH B: ACN 90 : 10 Isocratic

Flow: 0.2ml/min

Temp: Ambient

Wavelength: 254nm

1. 4-Nitroaniline

2. 2-Nitroaniline

3. Nitrobenzene

4. 2,6-Dinitrotoluene

Stereoisomers

1. Isoascorbic acid

2. Ascorbic acid

Decongestant


p/n: F18-050503

Mobile Phase: 90 : 10 H2O : MeOH + 0.1% NH3

Flow: 1.2ml/min

Temp: 25°C

Wavelength: 210nm

1. Phenylephrine

Column: Fortis HILIC 50x4.6mm 5µ

p/n: FHI-050305

Mobile Phase: 90 : 10 ACN : NH3OAc

Flow: 1ml/min

Temp: 25°C

Wavelength: 254nm

• Column : • p/n : • Mobile Phase:


Minutes0.0 1.0 2.0 3.0 4.0 5.0 6.0

60

80

100

120

140

Minutes

0

2

4

6

8

0

2

4

6

8

0.2

31

.79

22

9

3.3

51

.08

28

80

Minutes

50

Minutes

0

10

20

30

40

0

10

20

30

40

1.0

80

1.4

16

86

78

2.6

05

1.1

61

97

75

8

2.8

97

1.1

92

22

03

9

3.2

08

1.2

72

29

54

4

4.9

38

1.1

52

61

38

0

Minutes0 1 2 3 4 5 6 7 8 9 10

0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9

28

App

licat

ions

0 1.0 2.0 3.0 4.0 5.0 6.0


Applications

Proton Pump Inhibitor


p/n: F18-050503

Mobile Phase: A: H2O + 0.1% Formic acid B: ACN + 0.1% Formic acidGradient: 10 - 100% in 10minsFlow: 1.0ml/min

Temp: 25°C

Wavelength: 254nm

1. Omeprazole

Antiretroviral - HIV


p/n: F18-050705

Mobile Phase: A: H2O + Buffer B: MeOH + ACN

Flow: 1.0ml/min

Temp: 25°C

Wavelength: 215nm

Amprenavir

Non Steroid Anti-Inflammatory

1. Diclofenac Sodium

Anticonvulsant


p/n: F08-050705


Flow: 1.0ml/min

Temp: 25°C

Wavelength: 254nm

1. Lamotrigine


p/n: F18-050505


Temp: Ambient

Wavelength: 254nm

0.04

0.06

0.08

0.10

0.12

0.14

0.04

Minutes

Minutes0 1 2 3 4 5 6 7 8 9 10

200

400

600

800

1000

Minutes

Minutes0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10

0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10

29

Applications

Saquinavir

Nelfinavir

Atazanavir

Ritonavir

Lopinvir

IS


Applications

Antihistamine


p/n: F18-050505


Temp: 22°C

Wavelength: 254nm

1. Loratadine

Antipsychotic


p/n: F18-050305


Flow: 1.0ml/min

Temp: 25°C

Wavelength: 254nm

1. Haloperidol

Hypertension

1. Diltiazem

Antidepressant


p/n: F18-050303

Mobile Phase: A: H2O + 0.1% formic acid

B: ACN + 0.1% formic acidGradient: 10 - 100% in 10mins

Flow: 1.0ml/min

Temp: 20°C

Wavelength: 254nm

1. Fluoxetine

Column: Fortis H2o 50x4.6mm 5µ

p/n: FHO-050305


Flow: 1.0ml/min

Temp: 25°C

Wavelength: 254nm

0.05

0.10

0.15

0.20

0.25

0.05

0.10

0.15

0.20

0.25

Minutes

0.01

0.02

0.03

0.04

0.05

0.01

0.02

0.03

Minutes Minutes

0 1 2 3 4 5 6 7 8 9 10

0.05

0.06

0.07

0.08

0.09

0.05

Minutes0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10

0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 10

30

App

licat

ions

45 Coalbrookdale RoadClayhill Industrial ParkNeston Cheshire, UKCH64 3UG

t: +44 151 336 2266f: +44 151 336 2669 www.fortis-technologies.com e: [email protected]

Applications Index

For technical support or applications contact :

[email protected]

For more information VISIT :

www.fortis-technologies.com

11αHydroxyprogesterone 817αHydroxyprogesterone 82,4-D 202,4-DB 202,4-DCP 202,4-DP 202,6-Dinitrotoluene 292-Hydroxyestradiol 162-Nitroaniline 294-Ethylaniline 264-Hydroxyestradiol 164-Nitroaniline 295-HIAA 18Acetic acid 13Adenine 21Alkyl Benzenes 22Amitriptyline 9, 12, 32Amphetamine 15Amprenavir 30Ascorbic acid 29Atazanavir 30Atorvastatin 22Banvel 20Casein Tryptic Digest 27Cefachlor 5Cefadroxil 5Cefalexin 5Cefradine 5Ciprofloxacin 28Clopidogrel 19CMPP 20Cortisone 8Cytosine 21Dexamethasone 13Diazepam 23

Diclofenac Sodium 30Dihydroquinidine 17Diltiazem 31DOPAC 18Dopamine 18Doxazosin 29Ecstasy 15Fluoxetine 31Gliclazide 6Guanosine 21Haloperidol 31Hydroxy-21-acetate 8Isoascorbic acid 29Lamotrigine 19, 30Lopinavir 30Loratadine 31Lorazepam 23MCPA 20MCPB 20MDA (3,4-Methylenedioxyamphetamine) 15MDEA (3,4-Methylenedioxy-N-ethylamphetamine) 15MDMA (Ecstasy) 15Methaphetamine 15Methylmalonic acid 18Mirtazapine 23N,N-Dimethylaniline 26Napthalene 26Nelfinavir 30Neomycin Sulphate 13Nitrobenzene 29Nortriptyline 9, 12Omeprazole 30Organic acids 18Paroxetine 23PCOC 20

Pesticide 14PET Tracer 17Phenol 26Phenoxymethylpenicillin 28Phenylephrine 29PK11195 17Prednisolone 8Prednisone 8Prochlorperazine Maleate 27Progesterone 8Proguanil 27Protriptyline 9, 12Quetiapine 13Quinidine 17Raloxifene Glucuronides 13Ritonavir 30Rosuvastatin 22Saquinavir 30Serotonin 18Succinic acid 18Sulfamerazine 23Sulfamethoxazole 23Sulfathiazole 23Telmisartan 7Temazepam 23Theobromine 21Toluene 21Tramadol 28Trimipramine 9, 12Uracil 21, 26Uridine 21Valproate Semisodium 19Vitamin C 28

Fortis C18™, Fortis Phenyl™, Fortis H2o™, Fortis C8™, Fortis Cyano™, Fortis HILIC™, Fortis Pace™ are trademarks of Fortis™ Technologies Ltd.Sunfire® and Xterra® are registered trademarks of Waters Corporation. Luna®, Gemini® and Synergi® are registered trademarks of Phenomenex. Zorbax® Eclipse XDB® is a registered trademark of Agilent Technologies. ACE® is a registered trademark of ACT. Fortis is not associated with these companies. Comparative separations may not be representative of all applications. All columns are original manufacturers own. ©2008-2009 Fortis Technologies Ltd. All rights reserved.

Product Guide 2009 - hplc.eu · Steroids. Tel: +44 151 336 2266 2.5µm Fortis C18 Column Length 30...

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Transcript of Product Guide 2009 - hplc.eu · Steroids. Tel: +44 151 336 2266 2.5µm Fortis C18 Column Length 30...