Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143 S131

Producing extra-seasonal bulbous flowering plants of the genusHyacinths in the greenhouses

Bala Maria, Herea Narcisa, Tota Cristina, Petrescu Irina

Banat’s University of Agricultural Science and Veterinary Medicinefrom Timisoara, RomaniaE-mail address: hereanarcisa@yahoo.com (H. Narcisa).

Hyacinths are rustic bulbous plants much appreciated in the springlandscape together with other rustic bulbous plats such as tulip,narcissus, crocus and others, but also together with biennial plantssuch as Myosotis, Belis perennis and Viola Witrockiana. They arecultivated to produce either cut flowers or potted flowers for indoordecoration. In forced culture, they bloom in winter. In this paper,we present the procedure of obtaining flowering plants in winter.To obtain flowering plants for March 1st or 8th, we studied ninehyacinth varieties that we planted on three different dates, that is,January 9, January 16, and January 23, 2013. Trial results show thatplant height in the hyacinth variety Gipsy Queen had very signif-icantly positive differences on all three planting dates comparedto the trial mean. As for the length of the floral stem, the hyacinthvariety Woodstock had very significantly positive differences onplanting dates January 16 and January 23, 2013, compared to thecontrol variant.

http://dx.doi.org/10.1016/j.copbio.2013.05.417

Effects of cadmium contamination on the growth parametersof Capsicum sp.

Petrescu Irina, Dobosan Iulia, Madosa Emilian, Banu Constantin,Bala Maria

Banat’s University of Agricultural Science and Veterinary Medicinefrom Timisoara, RomaniaE-mail address: dobosan.iulia@yahoo.com (D. Iulia).

Cadmium (Cd) is a toxic heavy metal, causing phytotoxicity, and itsuptake and accumulation in plants pose a potential threat to humanhealth. The responses of plants to cadmium stress, depending onthe growth stages at which they were exposed to a stress factor.The current study is aimed mainly at determining the relationshipbetween Cd and growth parameters, and the possible difference inthe response to Cd toxicity between capsicum cultivars with dif-ferent Cd tolerance. We selected three paprika peppers genotypesvarieties were treated with 10 ppm Cd, 50 ppm Cd, and 100 ppmCd. Almost all selected growth parameters were sensitive to all orat least high doses of Cd. The plant height (cm), number of leavesand leaf area were negatively correlated with the cadmium con-centration. Based on the results we concluded that, these traits ofpaprika peppers plant are seriously affected by Cd treatment. Mostsensitive genotype was Cermei and most tolerant was Szegedy 180genotype.

Acknowledgement: The authors would like to thank theEuropean regional development fund (ERDF) to finance project“Biosensing of heavy metal pollution at both sides of Romanianand Hungarian border by agriculturally important capsicum plant”acronym BIOSENSOR No. HURO/0901/270/2.2.2.

http://dx.doi.org/10.1016/j.copbio.2013.05.418

Certification of wheat varieties using retrotransposons

Oxana Hapilina, Olesya Raiser, Damelya Tagimanova, AlmagulKakimzhanova

National Center for Biotechnology, Astana, KazakhstanE-mail address: oksfur@mail.ru (O. Hapilina).

Retrotransposons as molecular markers are widely used inthe different methods of the analysis: IRAP (Inter Retro-transposon Amplified Polymorphism), REMAP (Retrotransposon-Microsatellite Amplified Polymorphism). IRAP and REMAP aremarker systems based on long terminal repeats retrotransposons,developed for plants, that have been widely used for evolution,genetic diversity, DNA fingerprinting of cultivars and varieties,genetic mapping linkage. The purpose of research was an attemptto develop molecular-genetic passports for sorts and lines of wheatwith the use of both IRAP and REMAP markers. For detectionof genetic originality of 27 varieties of the soft wheat culti-vated in Kazakhstan, we used previously reported primers. Fourmost informative primers — 2 IRAP (Sukkula and Nikita), andalso two combinations of REMAP primers of (Sukkula + 8081,Sukkula + 8082) whose spectra were maximally saturated withhigh level of polymorphism (63.2–82.4%) were as a result selected.The sizes of the amplified fragments varied from 150 to 4000 bp.Selected primers were used to assess genomic polymorphism ofwheat varieties. The result of amplification was presented as a for-mula that characterizes polymorphism of every sort on the studiedIRAP-marker and REMAP-marker. Each primer was given the lettercoding. The amplified loci have been indexed in accordance withthe size of the fragments. These formulas can be used for develop-ment of molecular-genetic passports of lines and sorts of wheat.

http://dx.doi.org/10.1016/j.copbio.2013.05.419

NPR1 expression in sunflower infected with downy mildew

Tatiana Sestacova 1, Ion Gisca 2, Aliona Cucereavii 2, Angela Port 1,Maria Duca 1

1 University of the Academy of Sciences of Moldova, University Centerof Molecular Biology, Republic of Moldova2 Research Center AMG-Agroselect, Republic of MoldovaE-mail address: tatiana.shestacova@gmail.com (T. Sestacova).

NPR1 (Non-Expressor of Pathogenesis-Related 1) protein is acentral positive regulator of systemic acquired resistance (SAR),that plays an essential role in salicylic acid (SA)-mediated SAR,also being involved in crosstalk inhibition of jasmonic acid (JA)-mediated defense responses. Activation of NPR1 occurs under thereducing conditions caused by initial oxidative burst after theinduction of defense responses to different pathogens. Thus, theaim of this study was estimation of NPR1 expression levels forthree sunflower genotypes (parental lines of Drofa F1 combina-tion and Drofa F1 hybrid) in normal and infected with downymildew plants grown in field. Expression levels of NPR1 wereestimated with RT qPCR using Maxima SYBR Green/ROX qPCRMaster Mix (Fermentas). The NPR1 RNA primers were designedthrough Primer3Web v. 3.0.0. software from the Helianthus annuusmRNA partial coding sequence [GenBank: AY667500.1]. In the mostinfected genotype Drofa CMS was observed the highest expressionlevel of NPR1 gene. In systemic infected plants it was 5.56-fold,in medium infected plants 3.99-fold and in weakly infected plants1.72-fold higher than in control. The most resistant genotype wasDrofa Rf, that did not show plants with high or medium level ofattack. It was demonstrated that in heavy affected plants levels ofNPR1 expression are significantly higher than in healthy or weakly