Presentatie support documentMediqSuomiOy.pdf · Presentatie support document Author: Versie 1.0...
Transcript of Presentatie support documentMediqSuomiOy.pdf · Presentatie support document Author: Versie 1.0...
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Allergeenitestaus
Maria Nyqvist 10.5.2017
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PCR
ELISA
Lateral Flow
LC/MS
Available technologies for food allergen testing (I)
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Technologies for Allergen Analysis
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PCR
ELISA
Lateral Flow
LC/MS
Available technologies for food allergen testing (II)
Industrial laboratories, food & beverage
producers, ingredient suppliers, inspection
services, canteens, convenience stores,
consultants
Commercial & official laboratories, research
units, central industrial labs
Commercial & official laboratories, research
units
On-site testing: simple – reliable – rapid
Ready-to-use kits for 14 allergens
No toxic or allergenic substances
No analytical machines required
Technologies for Allergen Analysis
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RIDASCREEN® and RIDA®QUICK Gliadin products for gluten analysis:
Gluten
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AOAC-RI AOAC-OMA
RIDASCREEN® Gliadin – Official certificates
Gluten
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International Organizations recommend RIDASCREEN® R5 ELISAs (I)
AOECS
Association of
European Coeliac
Societies
recommends
R5 Sandwich ELISA
(Mendez method)
for natural and
heat-processed
foods
R5 ELISA
(competitive ELISA)
for hydrolyzed
gluten
TTB
Alcohol and Tobacco
Tax and Trade
Bureau / USA
TTB Ruling Number
2012-2:
Beer may only be
labeled as gluten-
free if the ‘R5
Mendez
competitive ELISA’
has been used
AOAC
publishes Official
Method of Analysis
Official MethodSM
number 2012.01:
RIDASCREEN®
Gliadin is the
only test kit
fulfilling the
requirements
MEBAK
Central European
Commission for
Brewing Analysis
recommends for
beer
RIDASCREEN®
Gliadin
competitive to
measure the gliadin
concentration
Gluten
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International Organizations recommend RIDASCREEN® R5 ELISAs (II)
AACCI
American Association of Cereal Chemists International
Method 38-50.01 (in process)
R5 Sandwich
RIDASCREEN® Gliadin
Method 38-55.01 (proposed)
R5 competitive
RIDASCREEN® Gliadin competitive
Gluten
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RIDA®QUICK
Gliadin
R5 antibody detects prolamin of
wheat, rye, barley
No cross reactivity to soya,
buckwheat or other gluten free
cereals
No hook effect
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RIDA®QUIK Gliadin – menetelmäkuvaus
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ELISA –menetelmän hyödyt ja haitat
• Tarkka, rinnakkaismittaukset kaikista näytteistä
• Todellinen kvantitatiivinen tulos
• Monta näytettä yhdellä mittauksella 18/42
• Isoissa näytemäärissä nopeampi kuin pikatestit
• Hitaampi (inkubaatioita väh. 30 min)
• Korkeammat pystytyskustannukset
• Vaatii näyteanalysoinnin uudelleenorganisointia
• Teknisesti haastavampi
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ELISA-määritys
4. Lisää 100µl näyttettä/standardia ja
inkuboi pöydällä 10 min
5. Pese kaivot 3 kertaa 250 µl
pesupuskuria/kaivo.
Lisää 100 µl laimennettua Conjugate-
liuosta ja inkuboi pöydällä 10 min
6. Pese kaivot 3 kertaa 250 µl
pesupuskuria/kaivo.
Lisää 100 µl Chromogen/Substrate-
liuosta ja inkuboi pimeässä 10 min
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7. Lisää 100 µl stop-liuosta
8. Mittaa absorbanssi 450
nm 10 min sisään.
Määrityksen herkkyys vaihtelee eri näytemateriaalien välillä.
Myös eristystapa vaikuttaa herkkyyteen.
ELISA-määritys
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Huomioitavia vaiheita
Näytevalmistus
Näytteiden ja standardien pipetointi
Pesut
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• Hyvin spesifinen, suora määritys allergisoivasta proteiinista
• Vähäiset ristireaktiot
• Soveltuu myös paljon prosessoidun elintarvikkeen analysointiin
• Tehokas eristys erilaisille näytteille ja allergeeneille
• Useamman näytteen seulonta mahdollista
• kvantitatiivinen
• Kaikki parametrit validoitu.
ELISAn edut
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Analytical test kits to ensure gluten-free food
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Kiitos!