Preliminary Results of the Soybean Cyst Nematode Survey · SCN Survey of Manitoba (2012/2013) •...
Transcript of Preliminary Results of the Soybean Cyst Nematode Survey · SCN Survey of Manitoba (2012/2013) •...
Preliminary Results of the 2012/2013 Manitoba Soybean Cyst Nematode SurveySoybean Cyst Nematode Survey
Mario Tenuta and Mehrdad MadaniDepartment of Soil Science
Dennis LangeDennis LangeMAFRD
CPS Manitoba Meeting Nov. 20, 2013
Life Cycle of a ( d l ) lSCN (Heterodera glycines) Female
SoilSource: Swiss Federal Research Station for Agroecology and Agriculture
Source: Swiss Federal Research Station for Agroecology and Agriculture
Root
Source: Sandra Sardanelli Univ. Maryland
Source: Albert Tenuta OMAFRA
Female Becomes Cyst Eventually d lRupturing and Releasing Eggs
Source: Kris Lambert Univ. Illinois
Source: Edward McGawley, Louisiana State University
Soil
Source: Edward McGawley, Louisiana State University
SCN in Minnesota (2009)SCN in Minnesota (2009)
SCN in North Dakota (2011)SCN in North Dakota (2011)
2011
Sources:NDSU/NASS/NDSC Survey
2010
20112011
Crop ConsultantsDiagnostic Lab SubmissionsAGVISE + Submitted samples
2007
2011
2010
2011
2010 20032010
201120092011
Soil Processing
SCN Survey of Manitoba (2012/2013)SCN Survey of Manitoba (2012/2013)
• 48 soybean fields sampled48 soybean fields sampled• > 3000 soil samples282 i l f i• 282 composite samples for processing
• Priority fields based on– Proximity to water courses from U.S. that flood– Number of soybean years– History of dry beans– Sampled prone areas of fieldsp p
35 fields sampled Oct/Nov 2012
13 fields sampled July/Aug 2013
Collection Areas for Two FieldsCollection Areas for Two Fields
ResultsResults
• 37 composite samples from 22 fields had cysts37 composite samples from 22 fields had cysts• Total of 60 cysts recovered26 d d• 26 cysts were not damaged
• 23 cysts had circumfenestrate vulval cone structures – Cactodera, Punctodera, Betulodera
• 3 cysts were bifenestrate – Heterodera
ResultsResults
• 15 circumfenestrate cysts had eggs or juveniles15 circumfenestrate cysts had eggs or juveniles• 1 bifenestrate cyst had eggs and juveniles
S i i ifi C• ITS sequencing, species‐specific PCR• Circumfenestrate cysts ITS matched Cactodera• Bifenestrate cyst ambiguous – Heterodera by morphology, SCN by 2/3 primer sets, Cactoderap gy, y / p ,by ITS sequencing
Juveniles and CystsJuveniles and Cysts
Circumfenestrate
Bifenestrate
ConclusionsConclusions
• Soybean fields primarily along Red River were Soybea e ds p a y a o g ed e e esampled
• SCN not confirmed in the fields• Cactodera present in extremely low abundance in about half of fields samples
• Cactodera poses no known pest threat• Resampled 6 fields Oct 2013 to obtain more undamaged bifenestrate and circumfenestrate cysts
• Propose to conduct a survey on light soils in i klWinkler‐Portage area
Acknowledgements• Soybean farmers• William Shaw Brad Sparling Patrick Finnsson• William Shaw, Brad Sparling, Patrick Finnsson, Megan Westphal for samplingF d MPGA MRAC A i lt d A i• Funders: MPGA, MRAC‐Agriculture and Agri‐Food Canada (CAAP program)
• Canada Research Chair Program in Applied Soil Ecology
Three Things Are ForeverThree Things Are Forever
• DiamondsDiamonds• TaxesSC• SCN